LOCUS       MR226452                8175 bp    DNA     circular  SYN 
DEFINITION  Usp36 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 36 (Usp36)
ACCESSION   NM_001033528
VERSION     NM_001033528.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4332..4423 
                     /label="Myc-DDK"  
     misc_feature    4453..5038 
                     /label="PolyA signal"  
     misc_feature    4510..4529 
                     /label="Restriction Enzyme"  
     misc_feature    5314..5986 
                     /label="ColE1"  
     misc_feature    6416..7210 
                     /label="Kan/Neo"  
     misc_feature    7245..7547 
                     /label="SV40 ori"  
     misc_feature    7633..8110 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4314..4320 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4314 
                     /label="Usp36"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTATAGTC GATAAGCTGA AGGAGGCTCT CAAGCCTGGC CGCAAGGACT 
     1081 CGGCCGAGGA TGGGGACCTG GGCAGGCTGC TGGCAGCCTC TGCCAAGAAG GTGCTTCTGC 
     1141 AGAGGATTGA GTTTGAGCCG GCCAGCAAGA GCTTCTCCTA CCAGCTGGAG TCCTTGAAGA 
     1201 GCAAATACGT ACTGTTGAGC GCCAGGGCTG AGGGAGCCAG TCGCCACAGG AGCGGAGATG 
     1261 AACTCCAGGC CAGGAAACCA GGCACCGAGC GTGTGTCTGG AAGTGGAGGC GACGGAGTCC 
     1321 CTGCCCCGCA GAAAGTGCTC TTCCCTGTGG AGCGTCTGTC CCTGAGGTGG GAACGTGTTT 
     1381 TCCGAGTTGG CGCAGGGCTG CACAACCTGG GTAACACCTG CTTCCTCAAC TCCACCATCC 
     1441 AGTGCTTGAC CTACACACCA CCTCTGGCCA ACTACTTGCT CTCCAAGGAG CACGCACGGA 
     1501 GCTGTCACCA AGGGGGCTTC TGCATGCTGT GTCTCATGCA AAACCACATG GTCCAGGCCT 
     1561 TTGCCAACAG CGGCAATGCT ATCAAGCCCG TCTCCTTCAT CAGAGACCTG AAAAAGATTG 
     1621 CCCGGCACTT CCGGTTCGGG AACCAAGAGG ATGCACACGA ATTCCTGCGG TACACCATTG 
     1681 ATGCGATGCA GAAGGCCTGC TTGAATGGCT ACGCTAAGTT GGATCGGCAG ACACAGGCTA 
     1741 CTACCCTGGT GCATCAGATC TTTGGAGGCT ATCTCAGGTC CCGAGTGAAG TGCTCTGTGT 
     1801 GCAAGAGTGT CTCAGACACA TACGATCCCT ACTTGGACAT AGCACTGGAG ATCCGGCAAG 
     1861 CTGCAAATAT TGTGCGCGCT CTGGAACTTT TTGTGAAGTC AGATGTCCTG AGTGGAGAGA 
     1921 ACGCCTATAT GTGTGCTAAG TGTAAGAAGA AGGTTCCAGC CAGCAAGCGC TTCACCATCC 
     1981 ATAGAACATC CAATGTCCTG ACTCTGTCCC TCAAGCGCTT CGCCAACTTT AGTGGGGGGA 
     2041 AGATCACCAA GGATGTTGGC TATCCAGAGT TCCTGAACAT CCGTCCATAC ATGTCGCAGA 
     2101 GCAGCGGTGA TCCTGTCATG TATGGGCTCT ATGCTGTCCT GGTGCACTCA GGCTACAGCT 
     2161 GTCACGCTGG GCACTACTAC TGCTATGTGA AGGCCAGCAA TGGACAGTGG TACCAGATGA 
     2221 ACGACTCCTT GGTCCATTCC AGCAATGTCA AGGTGGTTCT GAACCAGCAG GCCTACGTGC 
     2281 TCTTCTATCT GCGGATTCCA GGCTCTAAGA AAAGTCCTGA GGGCCCTGTC TCCAGAGTGG 
     2341 GCGCCACTCT CCCTAGCCGT CCCAAAGTTG TTCCAGAGCA CTCCAAGAAG AGCCCTGGCA 
     2401 ATGGGGTTGT TCCCTCCCCG CTGATGGCAA AGCGACAAGA CTCTGTAATG ATGAGAAAGC 
     2461 TCCCAGCCCC TGAGGAGGTT GGCGTACCTG TGTCCAGAAA TGGCTCCCTT CCTGGCCTGA 
     2521 AGTTACAGAA CGGATGTGCT CCTGCCAAGA CACCTGCCGG GTCCCCGTCC CCAAGACTCA 
     2581 CGCCCACGCC CACTCATATG CCCACCATTC TGGATGAGCC CGGAAAGAAG GTGAAGAAGT 
     2641 CAGCCCCACT GCAGTCTCTC ACCACATCTC CAACCACTTC TCAGGGCTCT CCTGGGACCG 
     2701 GCGAGTCCCG GAGCCAGCGG CCCGGCTCCT GGGCAAGCAG AGACACCATC TTCTCTACCT 
     2761 CCCCGAAGCT CCTGGCCAGA GCCATTACTA ACGGGCACAG GCTGAAGGGC GAAGGCAGTG 
     2821 GTGTGGACCT GGAGAAGGGG GATTCAAGCA GCTCCAGCCC CGAGCACTCT GCCAGCAGTG 
     2881 ACCCTGCCAA GGCCCCGCAG ACCGCAGAGA GCAGAGCTGC GCATGCCTGT GATTCTCAGG 
     2941 GAACAAACTG TCCCACCGCT GGCCATCCCA AAGCGCTGTT GAATGGAGTA GATGCCAAGA 
     3001 TGGTGAAGTT GAAGTCCCCG GCCCTGAGCA GCACCACAAC CGAGCCCACA AGCCTCATGT 
     3061 CTCCTCCACC AGCCAAAAAA CTGGCCCTGT CAGCCAAGAA GGCCAGCACC CTGCGGAGGG 
     3121 CGACCGGCAA TGACATCGGT TCACCTTCCC CCTCAGCATT CTGCGACCTC ACCTCCCCCA 
     3181 TGAAAGCCAC CCACCCCGTC GTTGCCTCCA CTGGGCCTGT CAGTAAGACC AGGACTGCTG 
     3241 CACCTGCCCC CCGACCGTCC ACCCACCCCC ACTCTGCATC TCTGTCCAGT AGTAGTGCCA 
     3301 AGCCCTTAGG GACATCAGAG CCACAGAGCT GCCGCCCCTC TGCCTGGACA CCCCTGCCTC 
     3361 AGGTCAATGG ACACTTCACG TCCCACTTAC ACCAACTGCC AGAGGCCAGC GAGGCCCTAC 
     3421 ACAGCCCCTC CAAAAAGAGG AAAAAGACCC CTAATGGAGA TCCCCAGAGA CTGGGCATCG 
     3481 ACACGCTCCT CCCACAGTGC CTCAGGGGAG CACCTGCAGC AGCACGCAGG AAGAGAAAGA 
     3541 AGAGGTGCTC GGAGGGTGAG GGTGCCACAG CTCCCAAGCA GGAAGGCCAA TTTCAGGACC 
     3601 AGTCTTGGAG TTCCGGGAGC CAGAAGGAAG AGGGTACGCA GCCCCAGGTA AATGGCCATC 
     3661 AAGTGAGTCA CATTCTGGAC AGTTACCATG TGAGCAGCAG GAAAAGGAGG AAGAGGAAAA 
     3721 GATCGGAAGG ACTCAGCCAG GAAGCCACCC CGTCGCAGGA CCTAATTCAG CACAGCTGCT 
     3781 CCCCTGTAGA CCACAGTGAG CCTGAGGCCA GGACAGAGTT GCAGAAGAAG AAGAAGAAGA 
     3841 AAAGAAGGAA AAGAAAGCCC GAGCCACAGC AGGATGAAGA GAGCAAGCAC CCAGGAGACC 
     3901 AGAGGAGCCC AAGGCCCAGT GTCACCCCAG TCCCTGCGTT GAGTGTGAAT GGCCATCTTC 
     3961 CTAGTGACTG CTTGGGGCTG GGACAAGCAC CTCTCGTTAC CTGGAACAGA GATCAGGAAC 
     4021 CCGATGTGGT CCAGGCGTTG CTTCAGGACT CATCTGATAA GGCTTATGGG AAAAAAGTTT 
     4081 TGACCTGGGA TGGCGAGCCT TCAGCCATCA GTCAGGACGC CATCAAAGAC AGCAGACTAG 
     4141 CCCGGACCCA GACCGTGGTT GACGACTGGG ATGAAGAGTT TGATCGAGGG AAGGAAAAGA 
     4201 AAATTAAAAA GTTCAAGAGA GAAAAGAAGA GAAACTTCAA TGCCTTCCAG AAGCTTCAGA 
     4261 GTAGGCGCAA CTTTTGGTCT GTGACGCATC CTGCTAAGGT GGCCAGCCTC AGCTATCGCC 
     4321 GCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4381 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4441 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4501 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4561 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4621 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4681 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4741 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4801 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4861 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4921 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4981 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5041 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5101 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5161 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5221 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5281 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5341 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5401 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5461 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5521 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5581 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5641 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5701 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5761 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5821 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5881 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5941 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6001 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6061 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6121 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6181 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6241 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6301 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6361 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6421 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6481 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6541 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6601 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6661 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6721 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6781 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6841 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6901 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6961 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7021 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7081 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7141 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7201 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7261 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7321 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7381 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7441 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7501 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7561 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7621 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7681 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7741 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7801 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7861 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7921 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7981 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8041 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8101 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8161 TTTAACGCGA ATTTT
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