LOCUS       MR226445                7608 bp    DNA     circular  SYN 
DEFINITION  Usp33 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 33 (Usp33), transcript variant 1
ACCESSION   NM_133247
VERSION     NM_133247.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3765..3856 
                     /label="Myc-DDK"  
     misc_feature    3886..4471 
                     /label="PolyA signal"  
     misc_feature    3943..3962 
                     /label="Restriction Enzyme"  
     misc_feature    4747..5419 
                     /label="ColE1"  
     misc_feature    5849..6643 
                     /label="Kan/Neo"  
     misc_feature    6678..6980 
                     /label="SV40 ori"  
     misc_feature    7066..7543 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3747..3753 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3747 
                     /label="Usp33"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGACTTTT CGAAATCATT GTCCACATTT GGATTCAGTT GGTGAAATAA 
     1081 CGAAAGAGGA CTTGATACAG AAATCTCTCG GCGCTTGTCA GGACTGTAAA GTCAGAGGAC 
     1141 CAAACCTGTG GGCGTGCCTG GAGAATAGGT GCTCCTATGT TGGCTGCGGG GAGTCGCAAG 
     1201 TGGACCACAG CACCATACAC TCGCAGGAGA CAAAGCATTA TCTAACTGTG AACCTCACCA 
     1261 CTCTTCGAGT ATGGTGTTAT GCTTGCAGCA AAGAAGTATT TTTGGATAGA AAATTAGGAA 
     1321 CTCCTCCTTC ATTACCTCAT GTAAGACAGC CTCAACAAAC ACAAGAAAAC AGTGTCCAGG 
     1381 ATTTTAAAAT TCCCAGTAAT CCAGCATTGA AAACCCCCAT GGTTGCTGTG TCTGAAGATC 
     1441 TGGATATAGA AGTGGAAGAG GAAGACGAGC TGAAGGCTAG AGGCCTGACA GGTTTGAAAA 
     1501 ACATTGGAAA TACTTGTTAT ATGAACGCGG CGCTGCAGGC TCTTTCTAAC TGCCCACCTT 
     1561 TGACACAGTT CTTTCTTGAT TGTGGAGGAC TGGCTAGAAC AGATAAGAAA CCAGCAATTT 
     1621 GTAAAAGTTA TCTCAAACTA ATGACCGAGT TGTGGCACAA GAGCAGGCCA GGATCTGTTG 
     1681 TGCCGGCTAA TCTGTTCCAA GGAATTAAAA CTGTAAATCC AACTTTTCGA GGTTATTCTC 
     1741 AGCAGGATGC CCAGGAATTC CTTCGCTGTC TAATGGACCT GCTTCATGAG GAGCTGAAGG 
     1801 AGCAGGTCAT GGAAATGGAG GAAGAGCCTC AAACACTAAC TTCTGAGGAG ACGGTGGAGG 
     1861 AAGAGAAGAG CCAGTCAGAT GTGGATTTTC AGTCGTGCGA GTCTTGTAGC AGCAGCGAGA 
     1921 AAGCAGAAAA TGAGAGTGGC TCCAAAGGCT TTCCTGAGGA CAGCAATGAG ACCACCATGC 
     1981 TCATCCAGGA CGAGGATGAC CTGGAGATGG CCAAAGACTG GCAGAAAGAG AAGGTGTGCA 
     2041 ATAAGATCAA CAAGGCAAAT GCCGATGTAG AACTGGACAA AGACAGGGAC ACAGTGTGTG 
     2101 AAACAGTTGA CCTAAACAGC CAGGAGACCG TCAAAGTGCA GATACACGGC AGAGCCTCAG 
     2161 AATCTATCAC TGATGTCCAT CTGAATGACC TAGCTACGTC ACAGATCCTT CCTTCAAATG 
     2221 AAAGTGTTAG TCCACGGTTA TCAGCAAGCC CTCCTAAGCT AGGCAGTCTG TGGCCAGGAC 
     2281 TGTCGCCTCC ACACAAGAAA GCTCAGTCTA CATCTGCAAA GAGGAAAAAG CAGCATAAGA 
     2341 AATACAGAAG TGTCATCTCC GACATATTCG ATGGAACAGT CATTAGCTCG GTACAGTGTC 
     2401 TGACGTGCGA TAGGGTGTCT ATAACCCTCG AGACCTTTCA GGATCTGTCC TTGCCGATTC 
     2461 CTGGCAAGGA GGACCTGGCT AAGCTGCACT CCTCCAGTCA CCCAACTATA GTCAAAGCAG 
     2521 GGTCATGTGG TGAAGCGTAC GCCCCGCAGG GGTGGATAGC TTTCTTCATG GAGTATGTGA 
     2581 AGAGGTTTGT TGTCTCATGT GTCCCTAGCT GGTTTTGGGG TCCAGTAGTT ACCTTGCAAG 
     2641 ATTGTCTTGC TGCCTTCTTC GCCAGAGATG AACTTAAAGG TGACAACATG TACAGTTGTG 
     2701 AAAAATGCAA AAAGTTGAGG AATGGAGTAA AGTTTTGTAA AGTACAGAAG TTTCCTGAGA 
     2761 TTTTGTGTAT CCACCTTAAA AGATTTCGAC ATGAACTGAT GTTTTCCACC AAAATTAGCA 
     2821 CCCATGTTTC CTTCCCCCTG GAAGGCCTCG ATCTTCAGCC ATTTCTTGCA AAGGACAGCC 
     2881 CAGCTCAGAT TGTGACATAT GATCTCCTGT CAGTCATCTG TCACCATGGG ACTGCAAGTA 
     2941 GTGGGCACTA CATTGCCTAC TGCCGAAACA ATTTAAATAA CCTGTGGTAT GAGTTTGATG 
     3001 ACCAGAGCGT CACTGAAGTT TCAGAGTCCA CTGTACAGAA TGCCGAGGCC TACGTCCTTT 
     3061 TCTACAGGAA GAGCAGTGAA GAGGCACAAA AGGAGAGGCG GAGGATATCA AATTTGTTGA 
     3121 ACATCATGGA ACCTAGCCTC CTTCAGTTCT ACATATCTCG ACAGTGGTTG AATAAATTTA 
     3181 AGACCTTTGC TGAACCTGGC CCTATTTCAA ATAATGATTT TCTCTGTATC CATGGAGGTA 
     3241 TTCCTCCACG AAAAGCGAGT TATATTGAAG ACTTAGTTTT GATGCTGCCT CAGAACATTT 
     3301 GGGATAACCT CTATAGCAGG TATGGAGGAG GGCCTGCTGT CAACCATCTC TACATCTGCC 
     3361 ACACCTGCCA AATTGAGTTA GAGAAGATTG AAAAACGAAG AAAAACCGAA TTGGAAATTT 
     3421 TTATTCGGCT CAACAGAGCA TTTCAAGAGG AGGACTCCCC AGCTACTTTT TACTGTATCA 
     3481 GCATGCAGTG GTTTAGAGAA TGGGAGAGTT TTGTAAAGGG TAAGGATGGA GATCCCCCAG 
     3541 GTCCAATCGA CAACACTAAA ATTGCGGTTA CTAAATGTGG CAGTGTGATG CTCAAGCAAG 
     3601 GAGCAGACTC TGGTCAAATT TCAGAAGAAA CATGGAATTT CCTGCAGTCT ATATATGGTG 
     3661 GGGGGCCTGA AGTTATCCTC CGACCTCCAG TTGTTCATGT TGACCCTGAT GTACTCCAAG 
     3721 CAGAGGAAAA GATTGAAGTA GAAACTCGCT CTTTGACGCG TACGCGGCCG CTCGAGCAGA 
     3781 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3841 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3901 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3961 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4021 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4081 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4141 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4201 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4261 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4321 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4381 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4441 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4501 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4561 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4621 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4681 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4741 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4801 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4861 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4921 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4981 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5041 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5101 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5161 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5221 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5281 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5341 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5401 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5461 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5521 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5581 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5641 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5701 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5761 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5821 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5881 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5941 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6001 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6061 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6121 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6181 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6241 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6301 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6361 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6421 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6481 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6541 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6601 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6661 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6721 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6781 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6841 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6901 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6961 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7021 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7081 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7141 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7201 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7261 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7321 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7381 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7441 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7501 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7561 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//