LOCUS       MR226440                8037 bp    DNA     circular  SYN 
DEFINITION  Usp48 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 48 (Usp48)
ACCESSION   NM_130879
VERSION     NM_130879.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4194..4285 
                     /label="Myc-DDK"  
     misc_feature    4315..4900 
                     /label="PolyA signal"  
     misc_feature    4372..4391 
                     /label="Restriction Enzyme"  
     misc_feature    5176..5848 
                     /label="ColE1"  
     misc_feature    6278..7072 
                     /label="Kan/Neo"  
     misc_feature    7107..7409 
                     /label="SV40 ori"  
     misc_feature    7495..7972 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4176..4182 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4176 
                     /label="Usp48"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCCGCGG CTGCAGCTGG AGAAGGCGGC CTGGCGCTGG GCGGAGACGG 
     1081 TGCGGCCCGA GGAGGTGTCG CAGGAGCACA TCGAGACCGC CTACCGCATC TGGCTGGAGC 
     1141 CTTGTATCCG AGGCGTGTGC AGAAGAAACT GCAGAGGAAA CCCGAATTGC TTGGTTGGCA 
     1201 TTGGCGAGCA CATTTGGTTA GGAGAGATAG ATGAGAATAG TTTTCATAGC ATTGATGATC 
     1261 CCAACTGTGA GAGGAGGAAA AAGAATTCCT TTGTGGGCTT GACTAACCTG GGAGCCACCT 
     1321 GCTACGTCAA CACCTTTCTC CAGGTGTGGT TCCTCAACTT GGAGCTTCGG CAGGCGCTGT 
     1381 ACTTATGCCC CAGCACCTGC AGTGATTACA CGAAGGGAGA TGGGATCCAT GGGGGAAAAG 
     1441 ATTATGAGCC TCAGACAATT TGTGAGCATC TTCAGTACTT GTTTGCTTTG TTGCAAAACA 
     1501 GTAACAGACG ATACATCGAT CCTTCAGGAT TTGTTAAAGC CTTGGGCTTA GATACTGGGC 
     1561 AGCAGCAGGA TGCACAAGAG TTTTCCAAGC TCTTTATGTC GCTGTTGGAA GACACTTTGT 
     1621 CTAAACAGAA GAACCCGGAT GTTCGGAATG TTGTGCAGCA GCAGTTCTGT GGAGAATATG 
     1681 CCTATGTGAC TGTGTGCAAT CAGTGTGGCC GAGAGTCTAA GCTTGTATCG AAGTTCTATG 
     1741 AACTGGAGTT AAACATCCAA GGCCACAAGC AGTTAACAGA CTGCATCTCA GAGTTCCTGA 
     1801 AGGAAGAACG ATTAGAAGGA GACAATCGAT ACTTCTGTGA AAACTGTCAG AGCAAACAGA 
     1861 ATGCTACACG GAAAATCCGG CTTCTGAGCC TCCCTTGCAC TCTGAACTTG CAGCTGATGC 
     1921 GTTTCGTGTT TGACAGGCAA ACTGGACACA AGAAGAAGCT CAATGCATAC ATCGGCTTCT 
     1981 CAGAGAGTCT AGATATGGAG CCTTACGTGG AGCACAAAGG CGGGTCCTTC GTGTACGAGC 
     2041 TCAGTGCCGT CCTCATCCAC AGAGGAGTCA GCGCCTATTC TGGCCACTAC ATCGCACACG 
     2101 TGAAAGACCC TCAGTCTGGA GAATGGTACA AGTTCAATGA CGAGGACATA GAGAAGATGG 
     2161 AGGGCAAGAA GCTACAGCTA GGGATTGAGG AAGATCTAGA ACCTTCTAAA TCCCAGACCC 
     2221 GTAAACCCAA GTGTGGCAAA GGAACTCACT GCTCCCGAAA TGCATACATG CTGGTTTATA 
     2281 GGCTGCAAGC CCAGGAAAAG AACCACACAA TGGTTCAAGT CCCAGCCTTT CTTCAAGAGC 
     2341 TGGTAGATCG TGATAATTCC AAATTCGAGG AGTGGTGTGT AGAAATGGCC GAGATGCGCA 
     2401 AGCAGAGTGT GGACAAAGGC AAAGCGAAGC ACGAGGAGGT GAAGGAGCTG TACCAAAGGT 
     2461 TGCCTGCTGG AGCTGAGCCC TATGAGTTTG TCTCTCTTGA ATGGCTGCAG AAGTGGTTGG 
     2521 ACGAGTCAAC ACCCACCAAA CCGATTGATA ATAATGCTTG CCTGTGTTCC CACGACAAGC 
     2581 TTCATCCTGA TAAAATATCA ATTATGAAGA GAATATCGGA ATATGCGGCT AACATTTTCT 
     2641 ATAGTCGATA TGGAGGAGGT CCAAGACTAA CTGTGAAAGC CCTTTGTAAG GACTGCGTCG 
     2701 TGGAACGCTG CCGTGTCCTG CGCCTGAAGA ATCAGCTGAA TGAAGATTAC AAAGCTGTCA 
     2761 ACAATTTGCT GAAATCGACA ATGAAGGGTG ATGGATTTTG GGTAGGAAAG TCTTCCTTGC 
     2821 GTAGCTGGCG CCAGCTGGCT CTTGAACAAC TAGATGAGCA AGATGGTGAG GCGGAGCAAA 
     2881 GCAACGGGAA GATAAACGGG AGCACCTTCA ATAAAGATGA ATCAAAGGAA GAAAAGAAAG 
     2941 AAGAGGAGGA GGAATTAAAT TTTAATGAAG ACATTCTGTG TCCTCATGGA GAGTTAAGCA 
     3001 TATCTGAAAA TGAAAGAAGG CTGGTTTCTC AGGAGGCTTG GAGCAAGCTG CAGCAGTACT 
     3061 TCCCAAAGGC TCCCGAGTTC CCAAGTTACA GAGAGTGCTG CTCGCAGTGC AAGATACTAG 
     3121 AACGAGAAGG TGAAGAAAAC GAAGCCTTGC ATAAGATGAT AGCAAACGAG CAGAAGACCT 
     3181 CACTTCCAAA CTTGTTCCAG GACAAAAACA GACCATGTCT CAGTAACTGG CCAGAGGACA 
     3241 CGGATGCTCT CTATATTGTG TCGCACTTCT TTTTAGATGA ATGGCGGAAA TTTGTTAGAA 
     3301 AGCCTGCAAG GTCTGCTCCT GTGTCGTCAA TTGGGAATGC TGCCCTTCTG TGTCCACATG 
     3361 GAGGACTCAT GTTTACATTT CCTTCCCTCA CCAAAGAAGA CTCGAAACTC TGTCAACCTT 
     3421 TAGAGAGAAG AGAAGGAGGC TGGCCCACTG CAGCACATAA TAATATAGCT CTCATATGGC 
     3481 CCAGCGAATG GCAAATGATT CAAAAGCTCT TTGTTGTGGA TAAAGTAATT AAAATCACAA 
     3541 GAATTGAAGT GGGAGACGTG AATCCTTCTC AAACACAGTA TATTTCTGAA CCTAGTCTCT 
     3601 GTCCAGATTG CAGAGAAGGT CTGCTGTGCC AGCAGCAGAG GGACCTTCGA GAGTATACCC 
     3661 AAGCCACCAT CTATGTCCAC AAGGTTGTGG ATAACAAAAA GGTGATGAAG GACTCGGCCC 
     3721 CCGAGTTGAA TGTGAGTAGT TCTGAGACGG AAGAAGACAA GGAGGAGGCT AAGCCAGATG 
     3781 GGGAGAAGGA TCCTGACTTT AATCAAAGCA ATGGAAGTAC CAAGCGACAG AAGACATCCC 
     3841 AACAGGGCTA TGTGGCCTAC CAGAAGCAGG TCATCCGGAG GAGCACGAGG CACAGGAAGG 
     3901 TTCGGGGAGA GAAGGCACTG TTGGTGTCTG CCAACCAGAC GTTAAAAGAA TTGAAGATTC 
     3961 AGATCATGCA CGCATTTTCA GTTGCTCCAT TTGACCAGAA TTTGTCAATT GATGGAAAGA 
     4021 TTTTAAACGA CGACTGTGCC ACCCTTGGTA CCCTCGGCGT CATTCCTGAG TCTGTCATTT 
     4081 TGTTAAAGGC TGATGAACCA ATTGCAGACT ACGCTGCAAT GGATGATGTC ATGCAAGTTT 
     4141 GTATGCCGGA AGAGGGCTTT AAAGGTACGG GCCTGCTCGG ACATACGCGT ACGCGGCCGC 
     4201 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4261 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4321 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4381 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4441 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4501 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4561 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4621 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4681 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4741 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4801 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4861 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4921 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4981 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5041 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5101 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5161 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5221 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5281 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5341 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5401 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5461 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5521 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5581 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5641 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5701 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5761 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5821 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5881 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5941 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6001 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6061 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6121 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6181 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6241 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6301 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6361 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6421 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6481 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6541 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6601 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6661 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6721 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6781 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6841 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6901 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6961 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7021 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7081 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7141 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7201 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7261 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7321 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7381 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7441 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7501 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7561 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7621 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7681 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7741 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7801 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7861 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7921 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7981 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//