LOCUS       MR226432                8319 bp    DNA     circular  SYN 
DEFINITION  Usp40 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 40 (Usp40), transcript variant 2
ACCESSION   NM_001033291
VERSION     NM_001033291.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4476..4567 
                     /label="Myc-DDK"  
     misc_feature    4597..5182 
                     /label="PolyA signal"  
     misc_feature    4654..4673 
                     /label="Restriction Enzyme"  
     misc_feature    5458..6130 
                     /label="ColE1"  
     misc_feature    6560..7354 
                     /label="Kan/Neo"  
     misc_feature    7389..7691 
                     /label="SV40 ori"  
     misc_feature    7777..8254 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4458..4464 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4458 
                     /label="Usp40"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTTGGGAAC CTATTTGAAG AGGATTATTC CAGTGTGTCA AGTAGTCAGT 
     1081 ATGGAAGAGG GAAGAAATTA AAGACTAAAG GTTTGGAGCC GCCTGCCCCT AGAGAATTCA 
     1141 CCAATTTAAG TGGAATCAGA AATCAGGGTG GAACCTGTTA CCTCAGCTCC CTTCTTCAGA 
     1201 CGCTTCATTT CACACCTGAA TTCAGAGAAG CTTTATTTTC TCTTGGTCCG GAAGAGCTGG 
     1261 GTTCTTTAGA GGATAAAGAT AAACCTGATG CAAAGGTTCG GATCATACCT TTGCAGTTGC 
     1321 AGCGCTTGTT TGCCCAGCTT CTGCTCTTGG ACCAGGAGGC TGCATCTACC ATAGACCTCA 
     1381 CCGACAGCTT CGGGTGGACC AACGATGAGG AAATGAGGCA ACATGATGTG CAGGAACTCA 
     1441 ACCGAATTCT CTTCAGTGCT TTGGAAACTT CTTTAGTTGG GACCTCTGGC CATGACCTCA 
     1501 TTCATCGTCT ATACCATGGC ACCATTGTGA ACCAGATTGT TTGCAAAGAG TGTAAGAACA 
     1561 TCAGTGAGAG GCAGGAAGAC TTCTTGGATT TGACAGTAGC AGTCAAAAAT GTATCTGGTT 
     1621 TAGAAGATGA ACTCTGCAAC ATGTATGTGG AGGAAGAAAT TTTTGACTAT GACAACCTGT 
     1681 ACCACTGTGG AACCTGTGAC CGACTGGTTA AAGCAGCAAA GTCTGCCAAA TTGCGTAAGC 
     1741 TGCCTCCTTT TCTTACTATT TCATTACTAA GATTTAATTT TGATTTTGTG AAATGTGAAC 
     1801 GCTACAAGGA TACTAGCTGT TATACATTCC CCCTCCGGAT CAATCTCAAG CCTTTCTGTG 
     1861 AACAGAGTGA ACTGGATGAC ATGGAATATA TGTATGACCT CTTCTCCGTC ATTATCCACA 
     1921 AAGGTGGCTG CTATGGAGGT CATTACCATG TGTACATTAA AGATGTTGAT CATCTTGGCA 
     1981 ACTGGCAATG TCAAGAGGAG ATAAGTGATA CAAATGTGAA TGTGAAAGCT CCTCAGAGTG 
     2041 AAGAAGAGGC TAATGATCCA TTGGTGGTTC TAAAAACAAT CTTATTGCAG GAGGAGGCCA 
     2101 ATCAAATTCC TGTTGATCAA CTGGGCCAGA AACTCTTGAA AAAAACAGGA ATATCTTGGA 
     2161 ACAAGAAGTA CAGGAAACAG CATGGACCAC TGCGAAAGTT CTTACAGCTC CATCCTCAGA 
     2221 TATTTCTGCT CAGCACAGAT GAAAGTACAG TTAGTCTCCT GAGGAACCAT CTTACCCAGG 
     2281 CCCCATCTGA CCTACAGAGC TGTGAACAGA TTCTACATAC ACTTGCTTCA GAATCCCCAG 
     2341 GCTTGAATGA TGACACCAGT TGCCCACACT GGTTTGATAT CAATGATTCC AAAGTCCATC 
     2401 CAATCAGAGA GAAAGATATC ACACAGCAGT TCCAGGGTAA AGAAAGTGCC TACATGCTGT 
     2461 TTTATCGGAA AGCCACGTTA CAAAGGCCCC CTGAAGCTCG AGCCAATCCA AGATACAGAG 
     2521 TTCCATGTCA CTTGCTGAAG GAAATGGATG CAGCCAACAT TCTACTGCAG ATGAGAAGGG 
     2581 CAGAATGTGA CTCTGCAAAC AGTACTTTTG AGTTGCATCT TCATTTGGGT CCTCACTACC 
     2641 GTTTCTTCAA TGGGGCTCTT CACCCTGCAG TCTCTGAGAC AGAAAGCGTG TGGGACTTAA 
     2701 CGTTTGATAA AAGAAAAACT TTAGGAGATC TCCGTCAGTC AATATTTCAG CTATTGGAAT 
     2761 GTTGGGAAGG AGACATGGTT CTTAGTGTTG CGAAGCGTGT GCCAGCAGGA CTTCATGTGT 
     2821 ACCACACCCT CGATGGAGAT GACTTGACAC TGTGTGAAGC TGAGGTTGCT GATGGGGAAG 
     2881 ACATCTTTGT ATGGAATGGG GTAGAGGTTG GTGGAGTCCA AATTCAGACT GGTTTTGATT 
     2941 GTGAACCTCT GCTGTTAAAC ATTCTTCATC TAGAACTAAG TGGTGAAGGC TCAAAATGTG 
     3001 AGCAGTTGGT GGAGTCACCA CATGTCTTTC CTGCTAATGC AGAAGTGGGC GCTGTGTTCA 
     3061 CAGCCTTGGG CACCCCAGCA GGTGCCATCC TCATGAACAG TGTGGAGTCT GCAGATGGAG 
     3121 AGTGCTGGAC TGCTGTTCCC AAAGAAGACA TGAAGAAGAC GTTCAGAGAA CAAGGTCTCA 
     3181 GAAATGGAAG CTTAATTTTG GTTCAAGATT CAGACAGTGA TAATAACAGT CTGTTGCCCA 
     3241 AACAAGGGAG ATGGACCAAC AGCATGAATG AGCTCAACTG GCTCCAAGTG AAAAATTTCT 
     3301 GCCAGTCAGA ATCTGAAGAG AAGCAGGTTC AGATAGCCGT GACCATGCAC ACAGTGGTGT 
     3361 TTGATATTCG AATTAAAGCC ATAAAGGAAT TAAAATTAAT GAAGGAACTA GGAGAGATTT 
     3421 GGCACTTAAG GAAAATGGAT TGGTGCTATG AAGCTGGTGA ACCTTTATGT GAAGAGGATG 
     3481 CAACACTGAA AGAGCTTATG ATACGTTCTG GGGATACTTT GCTTTTAACT GAAGGGAAAC 
     3541 TTCCTCCTCC GGGTCACTTG AAGATGCCCA TCTGGTGGTA CCAGCCTGCA AGGCTGTCAG 
     3601 GACACTGTGA GAGTCGGGAC CACTTAAACT GTGCCTTTTC TCAAGATAGC ACCTGGAGGG 
     3661 CTGCTCCCAC CCAAGGTGCT CCTGGCCCCG AGCCTGCAGA AGTCTCTCTC CTCTACTTGG 
     3721 GGGATATGGA GATCTCAGAA GAGGCCACAT TGGTGGAACT GAAGTCTCAG GCCTTGGCCT 
     3781 TGCCTTCTGT TTCGAAGCTC GCTGTCCAGT CCACAGCCCT GCTCAGAGTC TGGACAGTGG 
     3841 AGAGCAAGCG CCCCAGCAGA CTCTTGCGCA CTAACTGGCG GCAGCTCAAG GAGTACAGAC 
     3901 TGGGCCGGAG AGCAGAGCTC TGCCTAGAGC TCCTTCAAAA AGAGGAGGAC TTGGGGCCCC 
     3961 GAGATGTACT GCTGAGGACA CAGCTACGCA TCCCTGGTGA GAGAGCCTAC TCCCTGGCGA 
     4021 CAGACCTGAT ATGGGACACC ACCAGAGGAT GGACTGCTGG CTCCTTGAGG CAGAGAGTAG 
     4081 CTGACTTCTA TTCTCTTCCT GTGGAGAAAA TTGAAATTGC CAAATATTTT CCTGAAAAGT 
     4141 TTGAGTGGCT TCCAATATCT AGCTGGAATC AGCAAGTTGC CAAAAGAAAA AAGAAGAAAA 
     4201 ACCAAGATAC TTTGCAAGGG GGACCATATT ACTTGAAAGA TGGAGACACT ATTGGCATTA 
     4261 AGAATCTCCT GTTTGATGAC AATGATGATT TCAGCACAAT CAGAGATGAC ATTGGAAAGG 
     4321 AGAACCAGAA GCGGCTAGCT TTGGAAAAAA AGAAAAGCCG AGAAGTCCAC CGTGCACAGA 
     4381 GCAGCGATTT GTTCTCCAAT GCAGGGGTGC CCGCTAGGTT CCGGGGACCA GAAGCTTCTC 
     4441 TGTCCATCCA CGTTGCAAGC TTCAGAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4501 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4561 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4621 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4681 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4741 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4801 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4861 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4921 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4981 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5041 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5101 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5161 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5221 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5281 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5341 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5401 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5461 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5521 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5581 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5641 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5701 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5761 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5821 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5881 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5941 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6001 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6061 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6121 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6181 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6241 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6301 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6361 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6421 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6481 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6541 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6601 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6661 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6721 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6781 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6841 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6901 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6961 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7021 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7081 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7141 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7201 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7261 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7321 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7381 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7441 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7501 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7561 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7621 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7681 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7741 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7801 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7861 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7921 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7981 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8041 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8101 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8161 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8221 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8281 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//