LOCUS       MR226425                8853 bp    DNA     circular  SYN 
DEFINITION  Usp19 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 19 (Usp19), transcript variant 2
ACCESSION   NM_001168371
VERSION     NM_001168371.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5010..5101 
                     /label="Myc-DDK"  
     misc_feature    5131..5716 
                     /label="PolyA signal"  
     misc_feature    5188..5207 
                     /label="Restriction Enzyme"  
     misc_feature    5992..6664 
                     /label="ColE1"  
     misc_feature    7094..7888 
                     /label="Kan/Neo"  
     misc_feature    7923..8225 
                     /label="SV40 ori"  
     misc_feature    8311..8788 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4992..4998 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4992 
                     /label="Usp19"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGCAGGG GCCAGTGCTA CAGGGCCCAG GAGGGGGCCG CCAGGACTGG 
     1081 AAGAGGCCAC TAGTAAGAAG AAACAGAAGG ATCGAGCAAA CCTGGAAAGT AAAGATGGAG 
     1141 ATGCTAGGAG AGTGTCCCTT CCTCGAAAGG AACCAACCAA AGATGAATTG TTGCTCGATT 
     1201 GGAGGCAGAG TGCAGATGAG GTGATTGTTA AGCTGCGCGT GGGAACAGGT CCCGTACGTC 
     1261 TGGAGGATGT AGATGCTGCG TTCACAGACA CGGACTGTGT GGTGAGGCTT CCAGATGGTC 
     1321 GGCAGTGGGG TGGTGTCTTC TTTGCTGAAA TACAAAGTTC TTGCACCAAA GTGCAGGCTC 
     1381 GCAAGGGTGG TCTTCTACAG CTAGTACTAC CCAAGAAGGT GCCTCTGCTC ACGTGGCCCT 
     1441 CTCTCCTGAA GAAACCTCTG GGAACCCAAG AGCTGGTGCC AGGTTTGCAG TGCCAGGAGA 
     1501 ACGGGCAAGA GCTGTCTCCC ATTGCCCTGG AGCCAGGCTC TGAGCCCCGC AGAGCTAAAC 
     1561 AGGAAGCCCG AAACCAGAAG CGGGCCCAGG GCCGTGGTGA GGTAGGCTCG GGGGCTGGCC 
     1621 CTGGGACACA GGCAGGGCCC AGCGCCAAGA GGGCTGTTCA CCTCCGCAGA GGGCCAGAAG 
     1681 GGGAAGGGTC CATGGATGGC CCCGGCCCCC AGGGTGATGC CCCGTCTTTC CTGTCTGACT 
     1741 CAGCTACCCA GGTTGAGGCT GAGGAGAAGC TCTGTGCTCC ACCAATGAAC ACTCAAACAA 
     1801 GTCTCTTGAG CTCAGAGAAG AGTTTAGCCC TTCTGACAGT AGAGAAGACA GTGTCCCCCA 
     1861 GGAATGACCC AGTCGCCCCG GTTATGGTCC AGGACAGAGA CCCTGAGCCT GAGCAAGAAG 
     1921 ACCAAGTCAA AGAGGAGATG GCACTTGGGG CTGATCCTAC AGCCTTGGTG GAGGAACCAG 
     1981 AGTCTATGGT GAACCTGGCA TTTGTCAAGA ACGACTCGTA TGAGAAGGGC CCGGATTCGG 
     2041 TGGTGGTGCA CGTGTACGTG AAGGAGATCC GCAGGGACAG CTCCCGAGTG CTCTTCCGAG 
     2101 AGCAGGACTT CACACTGATC TTCCAGACCA GGGACGGAAA CTTTCTGAGG CTGCATCCGG 
     2161 GCTGTGGGCC CCACACCATC TTCCGATGGC AGGTGAAGCT CAGAAACTTG ATTGAACCAG 
     2221 AGCAGTGTAC GTTCTGTTTC ACGGCCTCTC GAATCGATAT CTGCCTCCGG AAGCGGCAGA 
     2281 GTCAGCGCTG GGGGGGACTG GAGGCCCCTG CTACACGAGG TGCAGTGGGT GGTGCAAAGG 
     2341 TTGCCGTGCC GACAGGCCCA ACCCCTTTGG ATTCAACCCC TCCAGGAGGT GGCCCCCACC 
     2401 CCCTGACAGG CCAGGAGGAA GCCAGGGCTG TGGAGAAGGA AAAACCCAAG GCTCGATCAG 
     2461 AGGACTCAGG GCTGGATGGT GTGGTGGCCC GCACCCCCTT GGAGCATGTA GCCCCAAAGC 
     2521 CAGACCCACA CTTGGCCTCG CCCAAACCCA CGTGTATGGT GCCTCCAATG CCGCACAGTC 
     2581 CAGTTAGTGG GGATAGTGTG GAGGAGGACG AAGAGGAAGA GAAGAAGGTG TGCCTGCCAG 
     2641 GCTTCACTGG CCTTGTCAAC TTAGGGAACA CCTGCTTCAT GAATAGCGTC ATTCAGTCTT 
     2701 TGTCCAACAC TCGGGAACTT CGTGACTTCT TTCACGACCG ATCCTTTGAA GCTGAGATTA 
     2761 ACTACAATAA CCCATTGGGG ACTGGTGGGC GCCTCGCCAT TGGCTTTGCT GTGCTGCTCC 
     2821 GGGCCCTATG GAAGGGTACT CACCAAGCCT TTCAGCCCTC CAAGCTAAAG GCCATTGTGG 
     2881 CAAGCAAGGC CAGCCAGTTC ACAGGCTATG CACAGCATGA TGCTCAAGAG TTCATGGCTT 
     2941 TCTTGTTGGA TGGGCTACAT GAAGACCTCA ATCGAATCCA AAACAAACCC TACACAGAGA 
     3001 CTGTGGACTC GGACGGGCGG CCCGATGAGG TGGTAGCCGA GGAAGCATGG CAGCGGCACA 
     3061 AGATGAGAAA TGATTCATTC ATTGTGGACC TGTTTCAGGG CCAGTACAAG TCAAAGCTGG 
     3121 TGTGCCCTGT GTGTGCCAAG GTCTCCATCA CTTTTGACCC GTTCCTTTAT CTGCCGGTAC 
     3181 CCTTGCCACA AAAGCAAAAG GTTCTCCCCA TATTTTATTT TGCCAGGGAG CCCCACAGCA 
     3241 AGCCCATCAA GTTCCTGGTG AGTGTCAGCA AGGAGAACTC CAGCGCGAGT GAAGTGTTGG 
     3301 ACTCCCTCTC TCAGAGTGTC CACGTGAAGC CTGAGAACCT GCGCCTAGCC GAGGTAATTA 
     3361 AGAACCGTTT CCACCGTGTT TTCTTGCCCT CCCACTCACT GGACGCTGTG TCCCCCACGG 
     3421 ACGTGCTCCT CTGCTTTGAG CTGCTCTCCC CAGAACTGGC TAAGGAGCGG GTAGTAGTGC 
     3481 TGGAGGTGCA GCAGCGCCCC CAGGTACCCA GCATCCCTAT CTCCAAGTGC GCAGCCTGCC 
     3541 AGCGGAAGCA GCAATCAGAA GAAGAAAAGC TGAAGCGCTG TACCCGTTGC TACCGTGTGG 
     3601 GCTACTGCAA CCAGTTCTGC CAGAAAACCC ATTGGCCTGA CCACAAAGGC CTCTGCCGCC 
     3661 CTGAGAACAT TGGCTACCCC TTCCTGGTCA GTGTGCCTGC TTCACGCCTC ACTTATGCCC 
     3721 GTCTTGCTCA GCTACTAGAA GGTTATGCCC GGTACTCTGT GAGTGTATTC CAACCGCCCT 
     3781 TCCAGCCTGG CCGCATGGCT TTGGAATCGC AGAGCCCTGG CTGTACCACG TTGCTTTCAA 
     3841 CCAGCTCTCT GGAGGCTGGG GACAGTGAAA GAGAACCCAT TCAGCCTTCT GAGCTCCAGC 
     3901 TGGTGACCCC TGTGGCTGAA GGGGATACAG GGGCTCACCG AGTATGGCCG CCTGCTGATA 
     3961 GGGGTCCTGT GCCTAGCACC AGTGGACTCT CTTCTGAGAT GCTGGCCAGT GGGCCTATCG 
     4021 AAGGTTGTCC CTTGCTTGCT GGTGAGAGGG TATCTCGGCC TGAAGCTGCT GTGCCTGGGT 
     4081 ACCAACACTC AAGTGAATCT GTGAATACCC ACACGCCCCA GTTCTTCATC TATAAAATTG 
     4141 ATGCATCAAA CCGTGAGCAG CGGCTGGAGG ACAAAGGGGA GACACCATTG GAGCTAGGTG 
     4201 ATGACTGTAG CCTGGCTCTG GTGTGGCGGA ACAATGAACG CCTGCAGGAG TTTGTGTTGG 
     4261 TAGCCTCCAA GGAGCTGGAA TGTGCTGAAG ATCCAGGCTC TGCTGGTGAG GCTGCCCGTG 
     4321 CTGGCCACTT CACCCTGGAC CAGTGCCTCA ACCTCTTTAC ACGGCCTGAA GTGCTGGCAC 
     4381 CTGAGGAGGC CTGGTACTGC CCACAGTGCA AACAGCATCG TGAGGCCTCC AAACAGCTGC 
     4441 TGTTGTGGCG CCTACCGAAC GTGCTGATTG TGCAGCTCAA GCGCTTCTCC TTTCGTAGTT 
     4501 TCATTTGGCG AGACAAGATC AATGACTTGG TGGAGTTTCC TGTTCGGAAC CTGGACTTGA 
     4561 GCAAGTTCTG TATCGGTCAG AAAGAGGAGC AGCTGCCTAG CTATGACCTG TATGCTGTCA 
     4621 TCAACCACTA CGGAGGCATG ATCGGTGGCC ACTATACTGC TTGTGCACGG CTGCCCAATG 
     4681 ATCGCAGTAG CCAGCGCAGT GACGTGGGCT GGCGCTTGTT TGATGACAGC ACGGTGACAA 
     4741 CAGTAGACGA AAGCCAGGTC GTGACGCGCT ATGCCTATGT TCTCTTCTAC CGTCGTCGGA 
     4801 ACTCTCCTGT GGAGAGACCC CCCAGGGCAA GTCACTCTGA ACACCACCCA GACCTAGGCC 
     4861 CTGCAGCTGA GGCTGCTGCC AGCCAGGGAC TAGGCCCTGG CCAGGCCCCC GAGGTGGCCC 
     4921 CCACGCGGAC AGCCCCTGAA CGCTTCGCCC CCCCTGTGGA CCGCCCAGCC CCCACGTACA 
     4981 GCAACATGGA GGAGGTCGAT ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5041 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5101 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5161 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5221 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5281 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5341 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5401 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5461 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5521 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5581 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5641 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5701 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5761 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5821 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5881 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5941 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6001 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6061 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6121 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6181 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6241 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6301 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6361 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6421 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6481 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6541 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6601 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6661 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6721 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6781 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6841 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6901 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6961 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7021 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7081 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7141 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7201 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7261 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7321 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7381 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7441 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7501 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7561 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7621 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7681 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7741 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7801 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7861 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7921 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7981 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8041 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8101 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8161 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8221 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8281 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8341 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8401 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8461 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8521 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8581 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8641 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8701 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8761 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8821 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//