LOCUS       MR226331                9087 bp    DNA     circular  SYN 
DEFINITION  Robo3 (Myc-DDK-tagged) - Mouse roundabout homolog 3 (Drosophila) (Robo3)
ACCESSION   NM_001164767
VERSION     NM_001164767.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5244..5335 
                     /label="Myc-DDK"  
     misc_feature    5365..5950 
                     /label="PolyA signal"  
     misc_feature    5422..5441 
                     /label="Restriction Enzyme"  
     misc_feature    6226..6898 
                     /label="ColE1"  
     misc_feature    7328..8122 
                     /label="Kan/Neo"  
     misc_feature    8157..8459 
                     /label="SV40 ori"  
     misc_feature    8545..9022 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5226..5232 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5226 
                     /label="Robo3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGCGCTAC CTGCTTAAAA CACTACTACA GATGAACTTG TTCGCGGACT 
     1081 CCCTGGCCAG GGACATCTCC AACTCCAGCG AGCTACTCTT CGGCTTCAAC TCCTCGCTGG 
     1141 CAGCGCTCAA CCCTAGTCTG CTTCCTCCTG GAGATCCCTC TCTCAATGGG TCAAGAGTTG 
     1201 GGCCAGAAGA TGCCATGCCA CGCATCGTGG AGCAGCCGCC AGATCTGGTG GTTTCCAGGG 
     1261 GCGAGCCGGC TACTCTGCCC TGTCGTGCAG AAGGCCGGCC TCGACCCAAC ATCGAGTGGT 
     1321 ACAAGAATGG GGCGCGTGTG GCGACTGCAC GGGAGGATCC GCGTGCTCAC CGTCTGCTGC 
     1381 TGCCCAGCGG CGCCCTCTTC TTTCCCCGCA TTGTGCACGG GCGTCGCTCT CGGCCTGACG 
     1441 AGGGTGTCTA CACCTGTGTG GCTCGCAACT ACCTGGGAGC AGCGGCTAGC AGAAACGCCT 
     1501 CTCTGGAAGT AGCTGTCCTC CGTGATGATT TCCGGCAATC GCCTGGGAAC GTGGTGGTAG 
     1561 CGGTGGGGGA GCCAGCTGTA ATGGAATGTG TGCCCCCTAA GGGTCACCCA GAGCCTTTGG 
     1621 TGACCTGGAA GAAAGGCAAA ATAAAGCTTA AGGAAGAGGA GGGAAGGATC ACCATACGTG 
     1681 GAGGAAAGCT GATGATGTCA CACACGTTCA AGAGTGATGC TGGCATGTAT ATGTGCGTAG 
     1741 CCTCCAACAT GGCTGGAGAA CGAGAGAGTG GGGCAGCTGA ACTTGTGGTA CTGGAGCGTC 
     1801 CCTCATTTCT GCGTAGACCA ATAAACCAGG TTGTCCTGGC TGATGCCCCT GTGAATTTCC 
     1861 TGTGTGAGGT GCAGGGAGAT CCCCAGCCCA ATCTACACTG GCGCAAGGAT GATGGGGAGC 
     1921 TGCCCGCGGG CAGGTATGAG ATTCGGAGTG ACCACAGCCT TTGGATCGAC CAAGTGAGCT 
     1981 CTGAAGACGA GGGGACGTAC ACGTGTGTAG CAGAGAACAG TGTGGGCCGC GCAGAGGCAT 
     2041 CTGGCTCCCT CAGTGTTCAC GTCCCACCAC AATTTGTGAC TAAGCCCCAG AACCAGACAG 
     2101 TAGCTCCCGG AGCAAACGTG TCATTCCAAT GTGAGACCAA AGGAAACCCT CCACCTGCCA 
     2161 TCTTCTGGCA GAAGGAAGGG AGTCAAGTCC TGCTTTTCCC TAGTCAGTCA CTCCAACCCA 
     2221 TGGGACGCCT CTTAGTCTCT CCAAGAGGCC AGCTCAACAT CACTGAAGTA AAGATCGGGG 
     2281 ATGGTGGCTA CTACGTGTGC CAGGCTGTCA GTGTGGCTGG TAGCATCCTA GCAAAGGCCC 
     2341 TATTAGAGAT AAAAGGCGCC TCCATAGATG GGCTACCTCC CATCATTCTC CAGGGACCAG 
     2401 CCAATCAGAC ACTGGTACTT GGCTCTTCTG TGTGGCTGCC ATGCAGAGTG ATTGGAAACC 
     2461 CCCAGCCCAA CATCCAGTGG AAGAAGGATG AGAGGTGGCT GCAGGGGGAT GACTCACAGT 
     2521 TCAACTTAAT GGACAATGGC ACTCTACACA TTGCCAGCAT ACAGGAGATG GACATGGGTT 
     2581 TCTACAGCTG TGTGGCCAAG AGTTCCATAG GGGAGGCCAC ATGGAATAGC TGGCTTAGGA 
     2641 AGCAAGAAGA TTGGGGAGCA TCACCAGGTC CAGCAACAGG ACCCAGTAAT CCTCCAGGGC 
     2701 CTCCCTCTCA GCCAATAGTC ACAGAGGTAA CCGCAAACAG CATTACCTTG ACCTGGAAGC 
     2761 CCAATCCACA GTCTGGGGCC ACAGCTACCT CCTATGTGAT AGAGGCCTTC AGCCAAGCAG 
     2821 CTGGCAACAC GTGGCGGACG GTGGCCGATG GGGTGCAGCT GGAAACTTAC ACCATCAGTG 
     2881 GCCTGCAGCC CAACACCATC TACTTATTCC TAGTGAGAGC AGTTGGAGCC TGGGGCCTCA 
     2941 GTGAACCCAG TCCTGTCTCT GAGCCTGTTC AAACCCAGGA CAGCAGCCTA TCTAGGCCAG 
     3001 CGGAGGACCC GTGGAAAGGC CAGCGAGGGC TAGCGGAAGT GGCCGTGCGA ATGCAGGAGC 
     3061 CCACTGTCCT TGGGCCCAGA ACTCTGCAGG TTTCCTGGAC TGTGGATGGT CCAGTCCAGC 
     3121 TGGTGCAAGG ATTCCGTGTG TCTTGGAGGA TTGCAGGCCT TGACCAGGGA AGCTGGACAA 
     3181 TGCTAGACCT ACAGAGTCCA CACAAGCAAA GTACTGTGCT AAGAGGACTG CCCCCAGGGG 
     3241 CCCAAATCCA GATCAAGGTG CAAGTCCAAG GCCAGGAGGG GCTGGGGGCT GAAAGCCCTT 
     3301 TTGTGACCAG GAGCATTCCT GAGGAGGCCC CCAGTGGCCC CCCTCAGGGA GTGGCTGTGG 
     3361 CCTTGGGGGG TGATCGCAAC AGCAGTGTCA CTGTATCCTG GGAGCCTCCA CTTCCCTCCC 
     3421 AGCGAAATGG GGTCATCACT GAATACCAGA TTTGGTGCCT GGGTAATGAA AGCCGATTCC 
     3481 ACCTCAATCG ATCTGCAGCA GGTTGGGCAC GCTCTGTGAC GTTCAGCGGA CTGCTACCAG 
     3541 GCCAGATCTA TCGAGCCTTG GTGGCAGCAG CTACCAGTGC TGGCGTGGGC GTGGCCAGTG 
     3601 CTCCAGTGCT AGTGCAGCTG CCATTCCCTC CGGCGGCGGA GCCGGGGCCT GAGGTCAGCG 
     3661 AGGGGCTGGC AGAACGGTTG GCTAAGGTGC TGCGGAAGCC TGCTTTCCTA GCTGGCAGCA 
     3721 GCGCAGCCTG CGGGGCGCTG CTACTCGGGT TCTGCGCCGC CCTCTACCGG CGTCAGAAGC 
     3781 AGCGCAAAGA ACTCAGTCAC TATACGGCCT CCTTTGCCTA CACACCTGCA GTGTCCTTTC 
     3841 CACACTCAGA GGGCCTTTCT GGATCCAGTT CCAGGCCACC CATGGGCCTT GGCCCTGCTG 
     3901 CCTACCCATG GCTGGCAGAC TCCTGGCCCC ACCCACCTCG GAGTCCCTCA GCTCAGGAGC 
     3961 CCCGGGGGAG CTGCTGCCCC AGCAATCCTG ATCCAGATGA TAGATACTAC AATGAGGCAG 
     4021 GAATCTCCCT GTACTTGGCT CAGACTGCAC GTGGTGCTAA TGCCTCTGGT GAGGGTCCTG 
     4081 TCTACAGCAC CATTGACCCA GTAGGGGAAG AGCTACAGAC CTTCCACGGA GGGTTCCCTC 
     4141 AACACTCTTC TGGGGACCCA AGCACCTGGA GCCAGTATGC TCCCCCGGAA TGGAGCGAGG 
     4201 GGGACAGTGG AGCCAGGGGA GGCCAGGGGA AGCTTCTGGG CAAGCCTGTA CAAATGCCAT 
     4261 CTTTGAGCTG GCCAGAAGCC CTGCCACCGC CTCCCCCTTC CTGTGAGCTG AGCTGTCCGG 
     4321 AGGGGCCTGA GGAGGAGCTG AAGGGCAGTT CAGATCTGGA AGAGTGGTGC CCACCAGTGC 
     4381 CTGAGAAAAG CCACTTGGTT GGGTCAAGCT CCAGTGGTGC GTGCATGGTG GCTCCAGCCC 
     4441 CAAGGGACAC ACCCTCCCCC ACTTCTTCTT ATGGACAGCA GTCCACAGCT ACTCTTACCC 
     4501 CCTCACCTCC TGATCCTCCC CAACCCCCTA CTGACATCCC ACATCTCCAT CAGATGCCCA 
     4561 GGAGAGTACC CCTCGGGCCA AGTTCTCCTC TCAGCGTATC CCAGCCCGCT CTGAGTAGCC 
     4621 ATGACGGACG GCCTGTTGGC CTGGGTGCTG GCCCCGTACT CTCCTATCAC GCTAGCCCCA 
     4681 GTCCTGTCCC TAGTACAGCC AGCAGTGCAC CGGGGAGAAC CCGTCAGGTA ACTGGAGAGA 
     4741 TGACTCCCCC ACTCCATGGA CACCGTGCTC GAATCCGGAA GAAACCCAAG GCTCTTCCCT 
     4801 ACAGAAGGGA GCACAGTCCC GGGGACTTGC CCCCACCACC CTTGCCACCA CCAGAGGAAG 
     4861 AGACAAGCTG GCCCTTGGGT CTGCGGGCAG CAGGCAGCAT GTCTTCCCTA GAGCGAGAGC 
     4921 GCAGTGGGGA GAGGAGAGTG GTGCAGGCAG TGCCTCTAGG GGCCCAACCT CTAGGGGCCC 
     4981 AACGTGGGCC CCACCCAGAT GCTGCACTTC TGGGTTGTGC TGCGGAAGAG GCCTGGCTTC 
     5041 CATACGGCAG ACCAAGCTTC CTGAGCCATG GCCAGGGCAC CAGCACCTGC TCTACCGCTG 
     5101 GTAGCAATTC TTCTAGGGGT TCCAACAGCT CTCGAGGTTC TCGGGGCTCT CGGGGCCCTG 
     5161 GACGCAGCCG AAGTCGAAGC CGGAGCCAGA GCCAGAGCCA GAGGCCAGGA CGGAATCGCC 
     5221 GAGAGGAACC AAGAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5281 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5341 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5401 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5461 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5521 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5581 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5641 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5701 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5761 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5821 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5881 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5941 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     6001 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6061 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6121 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6181 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6241 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6301 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6361 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6421 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6481 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6541 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6601 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6661 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6721 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6781 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6841 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6901 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6961 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     7021 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     7081 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7141 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7201 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7261 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7321 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7381 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7441 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7501 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7561 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7621 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7681 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7741 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7801 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7861 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7921 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7981 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     8041 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     8101 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8161 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8221 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8281 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8341 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8401 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8461 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8521 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8581 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8641 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8701 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8761 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8821 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8881 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8941 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     9001 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     9061 ATTTAACAAA AATTTAACGC GAATTTT
//