LOCUS       MR226319                7740 bp    DNA     circular  SYN 
DEFINITION  Gaa (Myc-DDK-tagged) - Mouse glucosidase, alpha, acid (Gaa), transcript variant 1
ACCESSION   NM_008064
VERSION     NM_008064.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3897..3988 
                     /label="Myc-DDK"  
     misc_feature    4018..4603 
                     /label="PolyA signal"  
     misc_feature    4075..4094 
                     /label="Restriction Enzyme"  
     misc_feature    4879..5551 
                     /label="ColE1"  
     misc_feature    5981..6775 
                     /label="Kan/Neo"  
     misc_feature    6810..7112 
                     /label="SV40 ori"  
     misc_feature    7198..7675 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3879..3885 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3879 
                     /label="Gaa"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATATACGG AAGCCCCTCT GTTCGAACTC CGTGGTTGGG GCCTGCACCC 
     1081 TTATCTCTCT GACTACAGCG GTCATCCTGG GTCATCTCAT GCTTCGGGAG TTAATGCTGC 
     1141 TTCCCCAAGA CCTTCATGAG TCCTCTTCAG GACTGTGGAA GACGTACCGA CCTCACCACC 
     1201 AGGAAGGTTA CAAGCCAGGG CCTCTGCACA TCCAGGAGCA GACTGAACAG CCCAAAGAAG 
     1261 CACCCACACA GTGTGATGTG CCCCCCAGCA GCCGCTTTGA CTGTGCCCCC GACAAAGGCA 
     1321 TCTCACAGGA GCAATGCGAG GCCCGCGGCT GCTGCTATGT CCCAGCAGGG CAGGTGCTGA 
     1381 AGGAGCCGCA GATAGGGCAG CCCTGGTGTT TCTTCCCTCC CAGCTACCCA AGCTACCGTC 
     1441 TAGAGAACCT GAGCTCTACA GAGTCGGGGT ACACAGCCAC CCTGACCCGT ACCAGCCCGA 
     1501 CCTTCTTCCC AAAGGATGTG CTGACCTTAC AGCTGGAGGT GCTGATGGAG ACAGACAGCC 
     1561 GCCTCCACTT CAAGATCAAA GATCCTGCTA GTAAGCGCTA CGAAGTGCCC CTGGAGACCC 
     1621 CACGTGTGCT GAGCCAGGCA CCATCCCCAC TTTACAGCGT GGAATTCTCA GAGGAACCCT 
     1681 TTGGAGTGAT CGTTCGTAGG AAGCTTGGTG GCCGAGTGTT GCTGAACACA ACCGTGGCCC 
     1741 CCCTGTTCTT CGCTGACCAG TTCCTGCAGC TGTCCACTTC CCTGCCCTCC CAGCACATCA 
     1801 CAGGCCTGGG GGAACACCTC AGCCCACTCA TGCTCAGCAC CGACTGGGCT CGTATCACCC 
     1861 TCTGGAACCG GGACACACCA CCCTCGCAAG GTACCAACCT CTACGGGTCA CATCCTTTCT 
     1921 ACCTGGCACT GGAGGACGGT GGCTTGGCTC ACGGTGTCTT CTTGCTAAAC AGCAATGCCA 
     1981 TGGATGTCAT CCTGCAACCC AGCCCAGCCC TAACCTGGAG GTCAACGGGC GGGATCCTGG 
     2041 ATGTGTATGT GTTCCTAGGC CCAGAGCCCA AGAGCGTTGT GCAACAATAC CTGGATGTTG 
     2101 TGGGATACCC CTTCATGCCT CCATACTGGG GCCTCGGCTT CCACCTCTGC CGCTGGGGCT 
     2161 ACTCCTCGAC CGCCATTGTC CGCCAGGTAG TGGAGAACAT GACCAGGACA CACTTCCCGC 
     2221 TGGATGTGCA ATGGAATGAC CTGGACTACA TGGACGCCCG AAGAGACTTC ACCTTCAACC 
     2281 AGGACAGCTT TGCCGACTTC CCAGACATGG TGCGGGAGCT GCACCAGGGT GGCCGGCGCT 
     2341 ACATGATGAT CGTGGACCCT GCCATCAGCA GCGCAGGCCC TGCTGGGAGT TACAGGCCCT 
     2401 ACGACGAGGG TCTGCGGAGG GGTGTGTTCA TCACCAACGA GACTGGGCAG CCGCTGATTG 
     2461 GGAAGGTTTG GCCCGGAACC ACCGCCTTCC CTGATTTCAC CAACCCTGAG ACCCTTGACT 
     2521 GGTGGCAGGA CATGGTGTCT GAGTTCCACG CCCAGGTGCC CTTCGATGGC ATGTGGCTCG 
     2581 ACATGAACGA ACCGTCCAAC TTCGTTAGAG GCTCTCAGCA GGGCTGCCCC AACAATGAAC 
     2641 TGGAGAACCC CCCCTATGTG CCCGGGGTGG TTGGCGGGAT CTTGCAGGCA GCCACCATCT 
     2701 GTGCCTCCAG CCACCAATTC CTCTCCACAC ACTACAACCT CCACAACCTG TACGGCCTCA 
     2761 CTGAAGCTAT CGCCTCCAGC AGGGCCCTGG TCAAGACTCG GGGAACACGA CCCTTTGTGA 
     2821 TCTCCCGCTC AACCTTCTCG GGCCACGGCC GGTACGCTGG TCACTGGACA GGGGATGTGC 
     2881 GGAGCTCTTG GGAGCATCTT GCATACTCTG TGCCAGACAT CCTGCAGTTC AACCTGCTGG 
     2941 GCGTGCCCCT GGTCGGGGCG GACATCTGCG GCTTCATAGG AGACACGTCA GAAGAGCTGT 
     3001 GTGTGCGCTG GACCCAGTTG GGGGCCTTCT ACCCCTTCAT GCGGAACCAC AATGACCTGA 
     3061 ATAGCGTGCC TCAGGAGCCG TACAGGTTCA GCGAGACGGC GCAGCAGGCC ATGAGGAAGG 
     3121 CCTTCGCCTT ACGCTATGCC CTTCTGCCCT ACCTGTACAC TCTCTTCCAC CGCGCCCACG 
     3181 TCAGAGGAGA CACGGTGGCC CGGCCCCTCT TCCTGGAGTT CCCTGAGGAT CCCAGCACCT 
     3241 GGTCTGTGGA CCGCCAGCTC TTGTGGGGGC CGGCCCTGCT CATCACACCT GTGCTTGAGC 
     3301 CTGGGAAAAC TGAAGTGACG GGCTACTTCC CCAAGGGCAC GTGGTACAAC ATGCAGGTGG 
     3361 TGTCAGTGGA TTCCCTCGGT ACTCTCCCTT CTCCATCATC GGCTTCATCC TTCAGATCTG 
     3421 CTGTCCAGAG CAAGGGGCAG TGGCTGACAC TGGAAGCCCC ACTGGATACC ATCAACGTGC 
     3481 ACCTGAGGGA GGGGTACATC ATACCGCTGC AGGGTCCCAG CCTCACAACC ACGGAGTCCC 
     3541 GAAAGCAGCC CATGGCTCTG GCTGTGGCAT TAACAGCAAG CGGCGAGGCC GATGGGGAGC 
     3601 TGTTCTGGGA CGACGGGGAG AGCCTTGCAG TTCTGGAGCG TGGGGCCTAC ACACTGGTCA 
     3661 CCTTCTCAGC CAAGAACAAT ACCATTGTGA ACAAGTTAGT GCGTGTGACC AAGGAGGGAG 
     3721 CTGAGCTACA ACTGAGGGAG GTGACCGTCT TGGGAGTGGC CACAGCTCCT ACCCAGGTCC 
     3781 TTTCCAACGG CATCCCTGTC TCCAATTTCA CCTACAGCCC TGACAACAAG AGCCTGGCCA 
     3841 TCCCTGTCTC ACTGCTGATG GGAGAGCTGT TTCAAATCAG CTGGTCCACG CGTACGCGGC 
     3901 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3961 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4021 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4081 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4141 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4201 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4261 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4321 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4381 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4441 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4501 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4561 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4621 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4681 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4741 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4801 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4861 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4921 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4981 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5041 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5101 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5161 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5221 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5281 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5341 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5401 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5461 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5521 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5581 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5641 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5701 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5761 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5821 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5881 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5941 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6001 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6061 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6121 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6181 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6241 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6301 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6361 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6421 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6481 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6541 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6601 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6661 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6721 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6781 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6841 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6901 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6961 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7021 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7081 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7141 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7201 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7261 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7321 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7381 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7441 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7501 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7561 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7621 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7681 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//