LOCUS       MR226241                8178 bp    DNA     circular  SYN 
DEFINITION  Slc12a6 (Myc-DDK-tagged) - Mouse solute carrier family 12, member 6 (Slc12a6), transcript variant 1
ACCESSION   NM_133648
VERSION     NM_133648.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4335..4426 
                     /label="Myc-DDK"  
     misc_feature    4456..5041 
                     /label="PolyA signal"  
     misc_feature    4513..4532 
                     /label="Restriction Enzyme"  
     misc_feature    5317..5989 
                     /label="ColE1"  
     misc_feature    6419..7213 
                     /label="Kan/Neo"  
     misc_feature    7248..7550 
                     /label="SV40 ori"  
     misc_feature    7636..8113 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4317..4323 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4317 
                     /label="Slc12a6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGCACTTC ACTGTGACCA AGGTAGAAGA CCCAGAGGAG GGGGCAGCTG 
     1081 GCCCCCTCTC TCCTGAGCCC AGCTCAGCAG AAGTAAAAGC CCGGATTCAG GATCCCCAAG 
     1141 AACCAGACCC GAGTCAGAAC TCCATCACAG GGGAGCACAG CCAGCTGTTA GATGACGGCC 
     1201 ATAAAAAAGC CCGAAATGCT TATCTCAATA ATTCCAACTA TGAAGAAGGA GACGAATATT 
     1261 TTGATAAAAA TTTGGCACTC TTTGAGGAAG AAATGGACAC CAGACCAAAG GTGTCTTCTC 
     1321 TCCTCAACCG CATGGCCAAC TATACAAATC TGACACAAGG AGCAAAGGAA CATGAAGAGG 
     1381 CAGAGAACAT CACTGAAGGG AAAAAGAAGC CTACCAAGAG CCCCCAAATG GGTACTTTCA 
     1441 TGGGTGTCTA CCTCCCATGT CTACAGAACA TCTTTGGAGT GATCCTCTTC CTGCGTCTTA 
     1501 CCTGGGTAGT GGGAACAGCT GGAATCCTTC AGGCCTTTGC AATTGTCCTC ATCTGCTGCT 
     1561 GCTGTACAAT GTTAACTGCC ATCTCCATGA GCGCCATCGC CACTAACGGA GTGGTGCCAG 
     1621 CTGGGGGCTC ATACTTCATG ATTTCCAGAG CCCTGGGCCC AGAGTTTGGC GGGGCTGTAG 
     1681 GCCTCTGCTT TTATCTTGGC ACCACATTTG CAGCAGCCAT GTATATTCTT GGTGCCATTG 
     1741 AAATCTTTCT GGTATACATT GTCCCCCGAG CTGCCATCTT TCGGAGTGAC GATGCACTCA 
     1801 AGGAGTCAGC AGCTATGCTG AACAACATGC GCGTCTATGG TACAGCCTTC TTGGTCCTCA 
     1861 TGGTCTTGGT GGTATTCATC GGCGTACGCT ATGTGAATAA GTTTGCCTCA CTCTTCCTGG 
     1921 CCTGTGTAAT TGTGTCGATC TTGGCTATCT ATGCTGGTGC CATCAAGTCT TCCTTTGCTC 
     1981 CACCACACTT CCCGGTCTGT ATGCTGGGCA ACCGTACCCT GTCATCAAGA CACCTTGACA 
     2041 TTTGCTCTAA GACCAAGGAG GTTGACAACA TGACAGTACC ATCAAAGTTA TGGGGATTCT 
     2101 TCTGCAACTC GAGTCAGTTC TTTAATGCCA CCTGTGATGA GTACTTTGTT CACAATAACG 
     2161 TCATCTCAAT CCAAGGCATT CCAGGGTTGG CTAGTGGTAT CATTACTGAA AATCTTTGGA 
     2221 GTAATTATTT ACCAAAGGGT GAGATAATTG AAAAGCCATC AGCCAAGTCA TCTGATGTCT 
     2281 TAGGCAACTT AAACCATGAA TATGTTCTTG CTGATATCAC CACCTCCTTC ACTCTGCTGG 
     2341 TGGGGATCTT CTTTCCCTCG GTCACAGGTA TCATGGCTGG GTCAAACAGA TCTGGAGATC 
     2401 TGAAAGATGC CCAGAAGTCT ATTCCCATTG GGACCATCCT TGCCATCCTG ACCACATCCT 
     2461 TTGTGTATTT AAGCAATGTT GTCCTTTTTG GTGCATGTAT TGAAGGAGTC GTTCTCAGAG 
     2521 ACAAATTTGG GGATGCTGTA AAAGGGAATT TGGTTGTAGG CACCTTATCC TGGCCATCCC 
     2581 CGTGGGTGAT CGTTATTGGC TCCTTCTTTT CAACATGTGG GGCTGGGCTG CAGAGCCTCA 
     2641 CAGGTGCGCC TCGGCTGCTG CAGGCTATCG CCAAGGATAA CATCATACCT TTCCTTAGGG 
     2701 TTTTTGGTCA CAGCAAAGCT AATGGGGAAC CTACCTGGGC TTTACTTCTA ACTGCTGCCA 
     2761 TAGCAGAGCT GGGAATTCTC ATCGCCTCCC TGGATCTCGT GGCCCCAATT CTTTCCATGT 
     2821 TTTTTCTCAT GTGTTACCTC TTTGTGAACT TGGCTTGTGC CTTGCAAACA TTGCTGCGAA 
     2881 CCCCCAACTG GAGGCCTCGA TTCCGCTATT ATCACTGGGC CCTCTCTTTC ATGGGAATGA 
     2941 GTATCTGTCT AGCTCTGATG TTCATTTCTT CTTGGTATTA TGCCATTGTA GCTATGGTAA 
     3001 TAGCTGGCAT GATCTACAAG TACATTGAAT ATCAAGGGGC TGAGAAAGAA TGGGGGGATG 
     3061 GTATCCGTGG GCTGTCGCTC AGTGCAGCCC GCTTCGCTTT GCTCCGTCTA GAGGAAGGAC 
     3121 CTCCTCACAC TAAAAACTGG AGGCCTCAGC TGCTCGTCCT ACTGAAGCTG GATGAAGATT 
     3181 TACACGTCAA GCACCCTCGC CTCCTCACCT TTGCCTCCCA GCTCAAGGCA GGAAAGGGAC 
     3241 TCACGATTGT GGGCTCTGTC ATCGTGGGGA ACTTCTTGGA GAACTATGGT GACGCGCTCG 
     3301 CGGCAGAGCA GACCATTAAG CACCTAATGG AGGCAGAAAA GGTAAAAGGA TTCTGCCAAT 
     3361 TGGTGGTGGC TGCCAAGCTG AAAGAGGGCA TTTCACACCT CATCCAGTCC TGTGGCCTCG 
     3421 GAGGCATGAA ACACAACACA GTGGTGATGG GCTGGCCCAA TGGCTGGCGT CAGAGTGAAG 
     3481 ATGCTCGCGC TTGGAAGACT TTCATTGGCA CAGTACGAGT GACAACTGCT GCCCATCTAG 
     3541 CCCTGCTGGT GGCTAAAAAT GTCTCCTTCT TTCCCAGCAA TGTGGAGCAG TTTTCTGAGG 
     3601 GCAACATTGA TGTGTGGTGG ATTGTGCATG ATGGGGGCAT GCTCATGCTA TTACCGTTCC 
     3661 TGCTGAAACA GCACAAGGTT TGGCGGAAAT GCAGCATACG GATCTTCACA GTAGCCCAAC 
     3721 TAGAAGACAA CAGTATCCAG ATGAAGAAGG ATCTGGCCAC CTTTCTGTAC CACCTGCGCA 
     3781 TTGAGGCAGA AGTGGAAGTG GTGGAGATGC ACGACAGTGA CATATCTGCC TATACATATG 
     3841 AGCGCACCCT GATGATGGAG CAGAGGTCCC AGATGCTTCG GCATATGCGG CTGTCCAAAA 
     3901 CAGAGCGAGA CAGGGAGGCA CAGCTGGTGA AAGATCGAAA CTCAATGCTA CGCTTGACCA 
     3961 GCATTGGCTC TGATGAGGAC GAAGAGACAG AAACGTACCA GGAGAAGGTG CACATGACTT 
     4021 GGACCAAGGA TAAATACATG GCATCCCGGG GGCAAAAGGT CAAGTCAATG GAAGGATTCC 
     4081 AGGACCTACT TAATATGCGT CCGGACCAGT CCAACGTGAG ACGGATGCAT ACAGCAGTGA 
     4141 AGCTCAATGA AGTTATAGTC AACAAGTCTC ATGAAGCAAA GCTGGTTTTG TTGAATATGC 
     4201 CAGGACCACC CCGGAACCCT GAAGGTGATG AAAACTACAT GGAATTTCTA GAAGTGCTCA 
     4261 CTGAGGGATT AGAACGAGTC CTTCTTGTCC GGGGTGGTGG CAGTGAGGTC ATCACCATTT 
     4321 ACTCAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4381 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4441 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4501 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4561 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4621 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4681 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4741 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4801 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4861 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4921 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4981 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5041 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5101 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5161 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5221 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5281 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5341 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5401 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5461 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5521 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5581 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5641 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5701 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5761 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5821 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5881 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5941 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6001 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6061 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6121 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6181 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6241 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6301 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6361 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6421 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6481 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6541 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6601 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6661 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6721 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6781 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6841 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6901 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6961 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7021 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7081 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7141 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7201 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7261 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7321 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7381 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7441 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7501 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7561 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7621 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7681 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7741 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7801 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7861 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7921 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7981 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8041 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8101 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8161 AAATTTAACG CGAATTTT
//