LOCUS       MR226235                9357 bp    DNA     circular  SYN 
DEFINITION  Neo1 (Myc-DDK-tagged) - Mouse neogenin (Neo1), transcript variant 1
ACCESSION   NM_008684
VERSION     NM_008684.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5514..5605 
                     /label="Myc-DDK"  
     misc_feature    5635..6220 
                     /label="PolyA signal"  
     misc_feature    5692..5711 
                     /label="Restriction Enzyme"  
     misc_feature    6496..7168 
                     /label="ColE1"  
     misc_feature    7598..8392 
                     /label="Kan/Neo"  
     misc_feature    8427..8729 
                     /label="SV40 ori"  
     misc_feature    8815..9292 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5496..5502 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5496 
                     /label="Neo1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGAG CGCGAAGCCG GGCGACTCCT CTGCACCTCC TCCTCCCGGC 
     1081 GCTGCTGTCC GCCACCGCCG CTGCTGCTGT TGCTGCCGCT GCTGCTGCTG CTCGGACGCC 
     1141 CGGCGTCCGG CGCCGCGGCC ACGAAGAGCG GCTCCCCGCC GCAGTCCGCA GGAGCCAGTG 
     1201 TTCGAACATT CACTCCGTTT TATTTTCTGG TGGAGCCAGT AGACACCCTC TCAGTTAGAG 
     1261 GCTCTTCTGT TATATTAAAT TGCTCGGCAT ATTCTGAGCC CTCTCCAAAC ATTGAATGGA 
     1321 AGAAAGATGG GACTTTTTTA AACTTAGAAT CAGATGATCG ACGCCAGCTA CTCCCAGATG 
     1381 GATCTTTATT CATCAGCAAC GTGGTGCATT CCAAACACAA TAAGCCTGAC GAAGGTTTCT 
     1441 ATCAGTGTGT AGCCACTGTG GATAATCTTG GAACCATTGT CAGCAGAACA GCCAAGCTCA 
     1501 CAGTAGCAGG TCTTCCAAGA TTTACCAGCC AACCAGAACC TTCTTCAGTC TATGTTGGAA 
     1561 ACAGTGCAAT TCTGAATTGT GAAGTTAATG CAGATTTGGT CCCATTTGTT AGGTGGGAAC 
     1621 AGAATCGACA GCCCCTTCTT CTAGATGACA GGATTGTCAA ACTTCCAAGT GGAACACTGG 
     1681 TTATCAGCAA TGCTACTGAA GGAGATGGGG GACTCTACCG CTGCATTGTT GAAAGTGGTG 
     1741 GGCCACCAAA GTTTAGTGAC GAAGCTGAAT TGAAAGTTCT TCAAGATCCT GAGGAAATTG 
     1801 TAGACTTGGT ATTTCTGATG CGACCATCTT CTATGATGAA AGTCACTGGT CAGAGTGCAG 
     1861 TGTTGCCATG TGTTGTCTCA GGGCTTCCTG CTCCAGTTGT TAGATGGATG AAAAACGAAG 
     1921 AAGTGCTTGA CACAGAAAGC TCTGGCAGGT TGGTCTTGCT AGCAGGAGGT TGCTTGGAGA 
     1981 TCAGTGATGT CACTGAGGAT GATGCTGGGA CTTATTTTTG CATAGCTGAT AATGGAAATA 
     2041 AGACAGTTGA AGCTCAGGCG GAGCTTACTG TGCAAGTGCC ACCTGGATTC CTGAAACAAC 
     2101 CTGCTAACAT ATATGCTCAC GAATCCATGG ACATTGTATT TGAATGTGAA GTCACTGGGA 
     2161 AGCCAACTCC AACTGTGAAG TGGGTCAAGA ATGGGGATGT GGTTATCCCC AGTGATTACT 
     2221 TTAAAATTGT AAAGGAACAT AATCTTCAAG TTTTGGGTCT GGTGAAATCA GATGAAGGGT 
     2281 TCTATCAATG CATTGCTGAG AATGATGTTG GAAATGCACA AGCTGGAGCC CAGCTGATAA 
     2341 TCCTTGAGCA TGATGTTGCC ATCCCAACAT TACCTCCCAC TTCACTGACC AGTGCCACTA 
     2401 CTGACCATCT AGCACCAGCC ACAACGGGAC CATTACCTTC AGCTCCTCGA GACGTCGTGG 
     2461 CCTCCCTGGT CTCTACTCGC TTCATTAAAT TGACATGGCG TACACCTGCA TCAGACCCTC 
     2521 ATGGAGACAA TCTCACCTAC TCTGTGTTCT ACACCAAGGA AGGGGTTGCT AGGGAGCGTG 
     2581 TTGAGAATAC CAGCCAGCCA GGAGAGATGC AGGTGACTAT TCAAAACTTG ATGCCAGCAA 
     2641 CTGTGTACAT CTTCAAAGTT ATGGCTCAAA ATAAGCATGG CTCTGGAGAA AGTTCAGCTC 
     2701 CTCTTCGAGT AGAGACACAG CCTGAGGTTC AGCTCCCTGG CCCAGCACCT AATATCCGTG 
     2761 CTTATGCAAC GTCACCTACT TCTATCACTG TCACCTGGGA AACACCGTTA TCTGGCAATG 
     2821 GGGAAATTCA AAATTACAAA TTGTACTACA TGGAAAAAGG AACTGATAAA GAACAGGATA 
     2881 TTGATGTTTC AAGTCACTCC TACACCATTA ATGGACTGAA GAAATACACA GAATACAGTT 
     2941 TCCGAGTGGT GGCCTACAAT AAACATGGTC CTGGAGTTTC TACACAAGAT GTTGCTGTTC 
     3001 GAACATTATC AGATGTTCCC AGTGCTGCTC CTCAGAATCT GTCCTTAGAA GTGAGAAATT 
     3061 CAAAGAGTAT AGTGATCCAC TGGCAGCCCC CTTCCTCAAC CACACAAAAT GGGCAGATAA 
     3121 CTGGCTACAA GATTCGATAT CGAAAGGCCT CCCGAAAAAG TGATGTCACT GAGACCTTGG 
     3181 TAACTGGGAC ACAGCTGTCT CAGCTGATTG AAGGTCTTGA TCGGGGGACA GAATATAACT 
     3241 TCCGAGTCGC TGCTCTCACA GTCAATGGTA CAGGTCCAGC AACTGATTGG CTGTCTGCTG 
     3301 AAACTTTTGA AAGCGACCTA GATGAAACTC GTGTTCCTGA AGTGCCCAGC TCTCTTCATG 
     3361 TCCGTCCGCT CGTCACTAGC ATTGTAGTGA GCTGGACTCC TCCAGAGAAC CAGAACATTG 
     3421 TGGTCCGAGG TTATGCCATC GGTTACGGCA TTGGCAGCCC TCATGCCCAG ACCATCAAAG 
     3481 TGGACTATAA ACAACGTTAT TACACCATCG AAAACTTGGA TCCAAGCTCT CATTACGTGA 
     3541 TTACCTTGAA AGCATTTAAC AATGTTGGCG AAGGCATCCC CCTTTATGAG AGTGCTGTGA 
     3601 CCAGACCTCA CACAGACACT TCTGAAGTTG ATTTATTTGT TATTAATGCT CCATACACTC 
     3661 CAGTGCCAGA TCCCACTCCC ATGATGCCAC CAGTGGGAGT TCAGGCTTCC ATTCTGAGTC 
     3721 ACGACACCAT AAGGATTACC TGGGCAGACA ACTCCCTGCC CAAACACCAG AAGATTACAG 
     3781 ACTCCCGCTA CTACACAGTC CGGTGGAAGA CCAACATCCC AGCAAACACG AAGTACAAGA 
     3841 ATGCAAATGC AACGACGTTA AGCTATTTGG TTACTGGTTT AAAGCCAAAT ACGCTCTATG 
     3901 AGTTCTCTGT GATGGTGACC AAAGGCAGAA GGTCAAGCAC GTGGAGTATG ACAGCTCATG 
     3961 GCGCTACCTT TGAATTAGTT CCTACTTCTC CACCTAAGGA TGTGACAGTT GTGAGTAAGG 
     4021 AAGGAAAACC TAGAACCATC ATAGTGAATT GGCAGCCTCC CTCTGAAGCT AACGGCAAGA 
     4081 TTACAGGTTA CATCATCTAT TACAGCACGG ATGTGAATGC AGAGATACAT GACTGGGTTA 
     4141 TTGAACCAGT TGTGGGAAAC AGACTGACTC ACCAGATTCA AGAGTTAACA CTTGATACGC 
     4201 CATACTACTT CAAAATCCAG GCCCGGAACT CAAAGGGCAT GGGGCCCATG TCTGAAGCTG 
     4261 TACAGTTCAG AACACCTAAA GCGGACTCCT CTGATAAAAT GCCTAATGAC CAAGCCTTAG 
     4321 GGTCAGCAGG AAAAGGAAGC CGACTACCAG ACCTGGGATC TGACTACAAA CCTCCAATGA 
     4381 GTGGCAGCAA CAGCCCTCAC GGGAGCCCCA CCTCCCCTCT GGACAGCAAC ATGCTGCTGG 
     4441 TCATCATTGT CTCTGTTGGC GTCATCACTA TCGTGGTGGT TGTGGTCATT GCTGTCTTTT 
     4501 GTACCCGGCG CACCACCTCT CACCAGAAGA AGAAACGAGC TGCGTGCAAA TCAGTGAATG 
     4561 GCTCCCATAA GTACAAGGGC AATTGCAAAG ATGTGAAGCC TCCAGACCTA TGGATCCATC 
     4621 ACGAGAGACT AGAGTTGAAG CCTATTGACA AGTCTCCAGA TCCTAACCCT GTCATGACTG 
     4681 ATACTCCAAT CCCTCGAAAC TCTCAAGATA TCACACCAGT GGACAATTCC ATGGATAGCA 
     4741 ATATCCATCA AAGGCGGAAT TCATACAGAG GGCATGAGTC AGAGGACAGC ATGTCTACAC 
     4801 TGGCTGGAAG GAGGGGAATG AGACCAAAAA TGATGATGCC CTTTGACTCT CAGCCACCTC 
     4861 AGCCTGTGAT TAGTGCCCAT CCCATCCATT CCCTCGATAA CCCTCACCAT CATTTCCACT 
     4921 CCAGCAGCCT CGCTTCTCCA GCCCGCAGTC ATCTCTACCA CCCAAGCAGC CCATGGCCCA 
     4981 TTGGCACATC CATGTCCCTT TCAGACAGGG CCAATTCCAC AGAATCTGTT CGAAATACCC 
     5041 CCAGCACGGA CACCATGCCA GCGTCCTCGT CTCAGACGTG CTGCACTGAC CATCAGGACC 
     5101 CTGAGGGTGC TACTAGCTCC TCTTACTTGG CCAGCTCCCA AGAGGAAGAC TCAGGCCAGA 
     5161 GTCTTCCCAC AGCCCATGTC CGCCCTTCCC ACCCTCTGAA GAGCTTCGCT GTGCCAGCAA 
     5221 TCCCACCCCC AGGACCTCCT CTCTATGATC CTGCACTGCC AAGCACACCA TTACTGTCCC 
     5281 AGCAAGCTCT GAACCATCAC ATTCACTCAG TGAAAACAGC CTCCATCGGG ACGTTAGGAA 
     5341 GGAGCCGGCC TCCTATGCCA GTGGTTGTTC CGAGTGCCCC TGAAGTACAG GAGACCACCA 
     5401 GGATGCTGGA AGACTCCGAG AGTAGCTATG AACCAGATGA GCTGACCAAA GAGATGGCCC 
     5461 ACCTGGAAGG ACTAATGAAG GACCTAAATG CCATCACAAC AGCCACGCGT ACGCGGCCGC 
     5521 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5581 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5641 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5701 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5761 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5821 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5881 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5941 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     6001 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     6061 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     6121 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     6181 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     6241 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     6301 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     6361 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     6421 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6481 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6541 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6601 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6661 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6721 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6781 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6841 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6901 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6961 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     7021 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     7081 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     7141 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     7201 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     7261 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     7321 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     7381 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     7441 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7501 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7561 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7621 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7681 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7741 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7801 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7861 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7921 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7981 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     8041 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     8101 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     8161 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     8221 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     8281 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     8341 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     8401 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     8461 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8521 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8581 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8641 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8701 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8761 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8821 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8881 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8941 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     9001 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     9061 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     9121 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     9181 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     9241 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     9301 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//