LOCUS MR226229L4 8858 bp DNA circular SYN DEFINITION Sphk1 (Myc-DDK-tagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 2 ACCESSION NM_025367 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 7149..7329 /label="3-LTR" misc_feature 833..1013 /label="5-LTR" misc_feature 8057..8703 /label="CAT(Cam-r)" misc_feature 2620..3234 /label="CMV" misc_feature 6024..6044 /label="LR50" misc_feature 4487..5215 /label="mGFP" misc_feature 5222..5287 /label="P2A" misc_feature 7377..7996 /label="pBR322 Ori" misc_feature 5288..5887 /label="Puro" misc_feature 3252..3271 /label="T7" misc_feature 3221..3237 /label="V2" misc_feature 3320..3328 /label="Restriction Enzyme" misc_feature 4466..4472 /label="Restriction Enzyme" misc_feature 3320..4466 /label="Sphk1" ORIGIN 1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAACCAGTA GAATGCCCTC GAGGACTGCT 3361 CCCACGGCCA TGCAGAGTGC TGGTGCTGCT GAACCCCCAG GGTGGCAAGG GCAAGGCTCT 3421 GCAGCTCTTC CAGAGCCGTG TGCAGCCCTT CCTGGAGGAG GCAGAGATAA CCTTTAAACT 3481 GATACTCACC GAACGGAAGA ACCATGCCAG GGAGCTGGTG TGTGCAGAGG AGTTGGGTCA 3541 CTGGGACGCC CTGGCAGTCA TGTCCGGTGA TGGTCTGATG CATGAGGTGG TGAATGGGCT 3601 AATGGAACGG CCCGACTGGG AGACTGCCAT CCAGAAACCC CTGTGTAGCC TCCCTGGAGG 3661 CTCCGGCAAT GCGCTGGCAG CTTCTGTGAA CCACTATGCT GGGTACGAAC AGGTGACTAA 3721 TGAAGACCTG CTCATCAACT GCACACTGCT GTTGTGCCGC CGGCGCCTGT CACCCATGAA 3781 CCTGCTGTCC CTGCACACTG CTTCTGGGCT GCGGCTCTAT TCTGTGCTCA GTCTGTCCTG 3841 GGGCTTTGTT GCTGACGTGG ACCTCGAGAG TGAGAAGTAC AGGCGCTTGG GGGAGATTCG 3901 TTTCACAGTG GGCACCTTCT TTCGCCTAGC AAGCCTGCGC ATCTACCAAG GCCAACTGGC 3961 CTACCTTCCT GTAGGAACTG TGGCCTCTAA GAGACCCGCC TCTACACTGG TGCAGAAGGG 4021 CCCCGTCGAC ACACACCTTG TTCCTCTGGA GGAGCCAGTG CCTTCTCATT GGACTGTGGT 4081 ACCAGAACAG GACTTTGTCC TGGTGCTGGT GCTGCTACAC ACCCACCTGA GCTCCGAGCT 4141 GTTTGCAGCA CCCATGGGCC GCTGTGAGGC TGGTGTTATG CATCTGTTCT ACGTACGTGC 4201 GGGGGTGTCA AGGGCTGCGC TGCTGCGCCT CTTCCTGGCC ATGCAGAAGG GCAAGCATAT 4261 GGAACTTGAC TGTCCATACC TGGTTCATGT GCCCGTGGTT GCTTTCCGCC TGGAGCCCAG 4321 GAGCCAGAGG GGCGTGTTTT CTGTGGATGG AGAGCTGATG GTATGTGAAG CTGTGCAGGG 4381 CCAAGTGCAC CCAAACTACC TTTGGATGGT CTGTGGCAGC AGAGATGCCC CATCCGGCCG 4441 GGACTCCCGG CGGGGGCCAC CTCCAGAAGA ACCAACGCGT ACGCGGCCGC TCGAGATGAG 4501 CGGGGGCGAG GAGCTGTTCG CCGGCATCGT GCCCGTGCTG ATCGAGCTGG ACGGCGACGT 4561 GCACGGCCAC AAGTTCAGCG TGCGCGGCGA GGGCGAGGGC GACGCCGACT ACGGCAAGCT 4621 GGAGATCAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTGGTGAC 4681 CACCCTCTGC TACGGCATCC AGTGCTTCGC CCGCTACCCC GAGCACATGA AGATGAACGA 4741 CTTCTTCAAG AGCGCCATGC CCGAGGGCTA CATCCAGGAG CGCACCATCC AGTTCCAGGA 4801 CGACGGCAAG TACAAGACCC GCGGCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG 4861 CATCGAGCTG AAGGGCAAGG ACTTCAAGGA GGACGGCAAC ATCCTGGGCC ACAAGCTGGA 4921 GTACAGCTTC AACAGCCACA ACGTGTACAT CCGCCCCGAC AAGGCCAACA ACGGCCTGGA 4981 GGCTAACTTC AAGACCCGCC ACAACATCGA GGGCGGCGGC GTGCAGCTGG CCGACCACTA 5041 CCAGACCAAC GTGCCCCTGG GCGACGGCCC CGTGCTGATC CCCATCAACC ACTACCTGAG 5101 CACTCAGACC AAGATCAGCA AGGACCGCAA CGAGGCCCGC GACCACATGG TGCTCCTGGA 5161 GTCCTTCAGC GCCTGCTGCC ACACCCACGG CATGGACGAG CTGTACAGGT CCGGACTCAG 5221 AGTTTGGGTA GGAAGCGGAG CTACTAACTT CAGCCTGCTG AAGCAGGCTG GAGACGTGGA 5281 GGAGAACCCT GGACCTATGA CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA 5341 CGTCCCCAGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA 5401 CACCGTCGAT CCGGATCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC 5461 GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT 5521 CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT 5581 GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC 5641 GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA 5701 GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG 5761 GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG 5821 CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG 5881 CAAGCCCGGT GCCTGATGTA CACCCAAACG GCCGGCCGCG GTCTGTACAA GTAGGATTCG 5941 TCGAGGGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATACAT GTTTAAGGGT 6001 TCCGGTTCCA CTAGGTACAA TTCGATATCA AGCTTATCGA TAATCAACCT CTGGATTACA 6061 AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT 6121 ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT 6181 CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC 6241 GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA 6301 CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA 6361 TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG 6421 TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT GCCACCTGGA 6481 TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT 6541 CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA 6601 GTCGGATCTC CCTTTGGGCC GCCTCCCCGC ATCGATACCG TCGACCTCGA TCGAGACCTA 6661 GAAAAACATG GAGCAATCAC AAGTAGCAAT ACAGCAGCTA CCAATGCTGA TTGTGCCTGG 6721 CTAGAAGCAC AAGAGGAGGA GGAGGTGGGT TTTCCAGTCA CACCTCAGGT ACCTTTAAGA 6781 CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG 6841 GAAGGGCTAA TTCACTCCCA ACGAAGACAA GATATCCTTG ATCTGTGGAT CTACCACACA 6901 CAAGGCTACT TCCCTGATTG GCAGAACTAC ACACCAGGGC CAGGGATCAG ATATCCACTG 6961 ACCTTTGGAT GGTGCTACAA GCTAGTACCA GTTGAGCAAG AGAAGGTAGA AGAAGCCAAT 7021 GAAGGAGAGA ACACCCGCTT GTTACACCCT GTGAGCCTGC ATGGGATGGA TGACCCGGAG 7081 AGAGAAGTAT TAGAGTGGAG GTTTGACAGC CGCCTAGCAT TTCATCACAT GGCCCGAGAG 7141 CTGCATCCGG ACTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT 7201 GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA 7261 GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA 7321 GTGTGGAAAA TCTCTAGCAG CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA 7381 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT 7441 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC 7501 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC 7561 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT 7621 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC 7681 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG 7741 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA 7801 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC 7861 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA 7921 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA 7981 GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC 8041 TCACGTTAAG GGATTTTGGT CATGATTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT 8101 GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA AACGGCATGA TGAACCTGAA 8161 TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG 8221 GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA CTCACCCAGG 8281 GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT 8341 CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT 8401 ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT 8461 GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC CATACGGAAC TCCGGATGAG 8521 CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT 8581 TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT CTGGTTATAG GTACATTGAG 8641 CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG 8701 TATATCCAGT GATTTTTTTC TCCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 8761 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 8821 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGAC //