LOCUS       MR226225                8400 bp    DNA     circular  SYN 
DEFINITION  Plcb1 (Myc-DDK-tagged) - Mouse phospholipase C, beta 1 (Plcb1), transcript variant 2
ACCESSION   NM_019677
VERSION     NM_019677.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4557..4648 
                     /label="Myc-DDK"  
     misc_feature    4678..5263 
                     /label="PolyA signal"  
     misc_feature    4735..4754 
                     /label="Restriction Enzyme"  
     misc_feature    5539..6211 
                     /label="ColE1"  
     misc_feature    6641..7435 
                     /label="Kan/Neo"  
     misc_feature    7470..7772 
                     /label="SV40 ori"  
     misc_feature    7858..8335 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4539..4545 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4539 
                     /label="Plcb1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGGGGCT CAGCCCGGAG TGCACGCCTT GCAACTCAAG CCCGTGTGCG 
     1081 TGTCCGACAG CCTCAAGAAG GGCACCAAAT TCGTCAAGTG GGATGATGAC TCCACTATTG 
     1141 TTACTCCAAT TATTTTGAGG ACTGATCCTC AGGGATTTTT CTTTTACTGG ACAGACCAGA 
     1201 ATAAGGAGAC GGAGCTGTTA GATCTCAGCC TTGTCAAGGA TGCCAGGTGT GGGAAGCACG 
     1261 CCAAAGCTCC CAAGGACCCC AAGTTGCGTG AACTTCTGGA TGTTGGGAAC ATCGGACACT 
     1321 TGGAACAGCG CATGATAACT GTGGTGTATG GACCAGACCT GGTGAACATT TCCCACTTGA 
     1381 ATCTTGTGGC TTTTCAAGAG GAAGTAGCCA AGGAATGGAC AAATGAGGTT TTCAGCTTGG 
     1441 CAACAAACCT GCTGGCTCAG AACATGTCCA GGGACGCATT TCTGGAGAAA GCATATACTA 
     1501 AGCTCAAGCT CCAGGTCACC CCAGAAGGCC GCATTCCTCT TAAAAACATC TATCGATTGT 
     1561 TCTCAGCAGA CCGGAAGCGC GTTGAAACTG CACTAGAGGC TTGTAGTCTT CCATCTTCGA 
     1621 GGAATGACTC CATTCCCCAA GAGGACTTCA CTCCAGATGT ATACAGAGTT TTCCTGAACA 
     1681 ATCTCTGTCC CCGACCTGAA ATTGATAACA TCTTTTCTGA ATTTGGTGCC AAAAGCAAAC 
     1741 CGTACCTGAC GGTTGATCAG ATGATGGATT TTATCAACCT TAAGCAGAGA GATCCCCGGC 
     1801 TCAATGAAAT CCTTTACCCA CCTCTGAAGC AGGAGCAGGT CCAAGTGTTG ATTGAGAAAT 
     1861 ATGAGCCCAA CAGCAGCCTC GCCAAGAAAG GGCAGATGTC AGTGGATGGA TTCATGCGCT 
     1921 ACCTGAGCGG AGAAGAAAAT GGAGTCGTTT CACCTGAGAA ACTGGATTTG AACGAAGACA 
     1981 TGTCTCAGCC CCTGTCTCAC TATTTCATCA ATTCCTCGCA CAACACCTAC CTCACAGCTG 
     2041 GCCAGTTGGC TGGGAACTCG TCTGTAGAGA TGTATCGCCA GGTGCTTCTG TCTGGATGTC 
     2101 GCTGTGTGGA GCTGGACTGC TGGAAGGGAA GGACCGCTGA GGAAGAGCCT GTCATTACCC 
     2161 ATGGATTCAC CATGACAACA GAAATATCCT TCAAGGAAGT CATAGAAGCC ATCGCAGAGT 
     2221 GTGCGTTCAA GACGTCTCCT TTCCCCATCC TCCTTTCCTT TGAGAACCAC GTGGATTCCC 
     2281 CGAAGCAACA AGCCAAGATG GCCGAGTATT GCCGATTAAT CTTTGGTGAT GCCCTCCTGA 
     2341 TGGAACCACT GGAAAAATAC CCACTGGAAT CTGGGGTACC TCTTCCAAGC CCTATGGATT 
     2401 TAATGTATAA AATCTTGGTG AAAAACAAGA AGAAGTCGCA CAAGTCGTCA GAGGGAAGTG 
     2461 GTAAGAAGAA GCTTTCAGAG CAAGCTTCCA ACACCTACAG CGACTCCTCC AGTGTGTTCG 
     2521 AGCCTTCGTC TCCAGGAGCT GGGGAAGCAG ATACGGAGAG TGATGACGAT GACGACGACG 
     2581 ATGACTGTAA AAAGTCTTCC ATGGATGAGG GGACAGCTGG CAGCGAAGCC ATGGCCACAG 
     2641 AAGAGATGTC TAACCTGGTG AACTATATTC AGCCTGTCAA GTTTGAGTCC TTTGAAATTT 
     2701 CAAAAAAAAG AAATAAAAGC TTTGAAATGT CTTCCTTCGT GGAAACCAAA GGACTTGAAC 
     2761 AACTTACAAA GTCTCCGGTG GAATTTGTAG AATACAACAA GATGCAACTT AGCAGGATAT 
     2821 ATCCCAAGGG AACACGTGTG GATTCATCCA ACTACATGCC TCAACTCTTC TGGAATGCTG 
     2881 GCTGTCAGAT GGTGGCGCTC AACTTCCAGA CAGTGGATCT AGCTATGCAG ATAAACATGG 
     2941 GCATGTATGA GTACAATGGG AAGAGCGGCT ATAGGCTGAA GCCAGAGTTC ATGAGGAGGC 
     3001 CGGACAAGCA TTTTGATCCA TTTACTGAAG GAATCGTAGA TGGGATAGTG GCCAACACTC 
     3061 TATCTGTTAA GATTATTTCA GGTCAGTTCC TCTCTGATAA GAAAGTTGGG ACTTATGTGG 
     3121 AAGTGGATAT GTTTGGCTTG CCTGTGGACA CAAGGAGGAA GGCATTTAAA ACCAAGACAT 
     3181 CCCAAGGAAA TGCTGTAAAC CCTGTCTGGG AAGAAGAACC TATTGTATTC AAAAAGGTAG 
     3241 TTCTACCTTC TCTGGCCTGT TTGAGGATAG CAGCATATGA AGAGGGAGGC AAATTTATTG 
     3301 GCCACCGGAT ATTGCCTGTG CAGGCTATTC GGCCAGGCTA TCACTACATC TGCCTGCGGA 
     3361 ATGAGAGGAA CCAGCCCCTG ACGCTGCCAG CTGTCTTTGT CTACATAGAA GTCAAAGATT 
     3421 ATGTGCCAGA CACGTATGCA GATGTAATCG AAGCTCTATC AAACCCAATC CGATATGTCA 
     3481 ATCTGATGGA ACAGAGAGCT AAGCAGCTGG CTGCGTTGAC CCTGGAGGAT GAAGAGGAAG 
     3541 TAAAGAAGGA GGCTGACCCT GGAGAAACAT CTTCCGAGGC TCCAAGCGAA ACCAGGACAA 
     3601 CGCCAGCAGA GAATGGGGTG AATCACACCG CATCCCTTGC ACCCAAGCCG CCTTCCCAGG 
     3661 CTCCACACAG CCAGCCTGCT CCAGGGTCTG TGAAGGCACC TGCCAAAACA GAAGATCTGA 
     3721 TCCAGAGCGT GTTAACAGAA GTAGAGGCGC AAACCATCGA AGAGCTCAAG CAACAGAAAT 
     3781 CGTTCGTGAA ACTTCAAAAG AAACACTACA AAGAAATGAA AGACCTGGTG AAGAGACACC 
     3841 ACAAGAAAAC CACGGAGCTC ATTAAGGAGC ACACGACCAA GTACAACGAG ATTCAGAATG 
     3901 ACTACCTGAG AAGGAGGGCA GCCTTGGAGA AGTCCGCCAA AAAGGACAGC AAGAAGAAAT 
     3961 CTGAACCCAG CAGCCCTGAC CACGGCTCAT CAGCCATTGA GCAAGACCTC GCTGCCCTGG 
     4021 ATGCAGAAAT GACTCAGAAG TTGATAGACT TGAAAGACAA GCAACAACAG CAGCTGCTTA 
     4081 ATCTTCGGCA AGAGCAGTAT TACAGCGAGA AGTACCAAAA GCGAGAACAC ATTAAACTGC 
     4141 TTATTCAGAA GTTGACAGAT GTTGCAGAAG AGTGTCAGAA CAATCAGTTA AAGAAGCTGA 
     4201 AGGAAATCTG CGAGAAAGAG AAGAAGGAAT TAAAGAAGAA AATGGATAAG AAGAGGCAAG 
     4261 AGAAGATAAC AGAAGCCAAG TCCAAAGACA AAAGCCAGAT GGAAGAGGAG AAGACAGAGA 
     4321 TGATCCGATC GTACATCCAG GAGGTGGTTC AGTACATCAA GAGGTTAGAG GAAGCACAAA 
     4381 GTAAAAGACA AGAAAAACTC GTGGAGAAAC ACAATGAGAT CCGCCAGCAG ATCCTCGATG 
     4441 AGAAGCCCAA GGGGGAAGGC CCCTCCTCAG TCTTGTCGGA AGGTTGCCAT GAGGATCCCT 
     4501 CTGTTCCCCC CAACTTTACT CCCCCCAACC CTCAAGCTCT CAAGTGGACG CGTACGCGGC 
     4561 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4621 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4681 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4741 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4801 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4861 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4921 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4981 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5041 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5101 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5161 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5221 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5281 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5341 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5401 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5461 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5521 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5581 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5641 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5701 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5761 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5821 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5881 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5941 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6001 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6061 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6121 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6181 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6241 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6301 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6361 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6421 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6481 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6541 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6601 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6661 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6721 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6781 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6841 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6901 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6961 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7021 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7081 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7141 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7201 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7261 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7321 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7381 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7441 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7501 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7561 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7621 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7681 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7741 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7801 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7861 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7921 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7981 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8041 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8101 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8161 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8221 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8281 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8341 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//