LOCUS       MR226222                7008 bp    DNA     circular  SYN 
DEFINITION  Capn3 (Myc-DDK-tagged) - Mouse calpain 3 (Capn3), transcript variant b
ACCESSION   NM_001109761
VERSION     NM_001109761.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3165..3256 
                     /label="Myc-DDK"  
     misc_feature    3286..3871 
                     /label="PolyA signal"  
     misc_feature    3343..3362 
                     /label="Restriction Enzyme"  
     misc_feature    4147..4819 
                     /label="ColE1"  
     misc_feature    5249..6043 
                     /label="Kan/Neo"  
     misc_feature    6078..6380 
                     /label="SV40 ori"  
     misc_feature    6466..6943 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3147..3153 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3147 
                     /label="Capn3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCTACCTG CTGCCGGGAT TCTTCTGTGA CAGGGTGATC CGAGAAAGGG 
     1081 ACAGGAGAAA TGGAGAGGGC ACTGTCTCAC AACCTCTCAA GTTTGAGGGG CAGGATTTTG 
     1141 TCGTTCTCAA ACAACAGTGT CTGGCTCAGA AGTGCCTCTT TGAAGACCGT GTCTTCCCAG 
     1201 CAGGTGTGCA GGCCCTTGGC TCCCATGAGC TGAGCCAGAA AGCCAAGATG AAGGCCATCA 
     1261 CTTGGAAGAG GCCAAAGGAA ATTTGTGAGA ATCCCCGGTT TATCATTGGT GGAGCCAACA 
     1321 GGACTGACAT CTGCCAAGGA GATCTAGGGG ACTGCTGGTT TCTTGCAGCC ATCGCCTGCC 
     1381 TGACCCTGAA TGAGCGGCTG CTTTTCCGAG TTATACCCCA TGATCAAAGT TTCACTGAAA 
     1441 ACTACGCAGG GATCTTCCAC TTCCAGTTCT GGCGCTATGG AGACTGGGTA GATGTGGTCA 
     1501 TTGACGACTG TCTGCCAACA TACAACAATC AGCTGGTTTT CACCAAATCC AACCACCGCA 
     1561 ATGAGTTCTG GAGTGCTCTC CTTGAGAAGG CTTATGCCAA GCTCCATGGC TCCTATGAAG 
     1621 CTCTGAAAGG TGGGAATACC ACAGAAGCCA TGGAGGACTT CACAGGAGGG GTGACAGAGT 
     1681 TTTTTGAGAT CAAGGATGCT CCGAGTGACA TGTACAAGAT TATGAGGAAA GCTATCGAGA 
     1741 GAGGCTCCCT CATGGGCTGC TCCATTGACA CAATTGTTCC AGTGCAGTAT GAAACAAGAA 
     1801 TGGCCTGTGG GTTGGTGAAA GGGCATGCCT ATTCAGTCAC TGGGCTGGAG GAGGCCCTGT 
     1861 TCAAAGGTGA GAAGGTGAAG TTGGTGCGGC TGCGGAACCC CTGGGGCCAG GTGGAGTGGA 
     1921 ACGGCTCTTG GAGTGATGGT TGGAAGGACT GGAGCTTTGT AGACAAAGAT GAGAAGGCCC 
     1981 GTCTACAGCA CCAGGTTACT GAGGATGGAG AGTTCTGGAT GTCATATGAT GACTTCGTCT 
     2041 ACCATTTCAC GAAGCTGGAG ATCTGCAACC TCACAGCTGA CGCCCTGGAG TCCGATAAGC 
     2101 TTCAGACCTG GACGGTGTCT GTAAACGAGG GCCGCTGGGT GAGGGGCTGT TCTGCTGGAG 
     2161 GCTGCCGAAA CTTCCCAGAC ACTTTCTGGA CCAACCCGCA GTACCGTCTC AAGCTTCTGG 
     2221 AGGAAGACGA TGACCCTGAG GACTCTGAGG TAATCTGCAG CTTCCTCGTG GCTCTGATGC 
     2281 AGAAGAACCG GCGCAAGGAC CGGAAGCTGG GGGCCAACCT CTTCACTATT GGCTTCGCCA 
     2341 TCTACGAGGT TCCCAAAGAG ATGCACGGGA ATAAGCAACA CCTGCAGAAG GACTTCTTCT 
     2401 TGTACAACGC CTCCAAGGCC AGAAGCAAAA CCTACATCAA CATGCGGGAG GTGTCCCAGC 
     2461 GCTTCCGCCT GCCACCCAGC GAGTATGTCA TCGTACCCTC CACCTACGAG CCCCACCAGG 
     2521 AGGGGGAATT CATCCTCCGA GTCTTCTCCG AAAAGAGGAA TCTCTCTGAG GAAGCTGAAA 
     2581 ATACAATCTC TGTGGATCGG CCAGTGCCAA GGCCTGGCCA CACAGACCAG GAAAGTGAGG 
     2641 AGCAGCAGCA ATTCCGGAAC ATCTTCAGGC AGATCGCAGG CGACGACATG GAGATCTGTG 
     2701 CAGATGAACT CAAGAATGTC CTCAACACAG TGGTGAACAA ACACAAGGAC CTGAAGACAC 
     2761 AAGGGTTCAC GCTGGAGTCC TGCAGAAGCA TGATAGCTCT CATGGATACA GATGGCTCTG 
     2821 GAAGACTGAA TCTTCAAGAG TTCCATCACC TCTGGAAAAA GATCAAGGCC TGGCAGAAAA 
     2881 TCTTCAAGCA CTATGACACA GACCATTCCG GTACCATCAA TAGCTATGAG ATGCGAAATG 
     2941 CAGTCAATGA TGCAGGCTTC CATCTCAACA GCCAACTCTA TGACATCATC ACCATGCGCT 
     3001 ATGCGGACAA ACACATGAAC ATCGACTTTG ACAGCTTCAT CTGCTGCTTC GTCAGGCTGG 
     3061 AAGGGATGTT CAGAGCTTTT AACGCATTTG ACAAGGATGG CGATGGTATC ATCAAACTGA 
     3121 ATGTACTTGA GTGGCTGCAG CTTACCATGT ATGCCACGCG TACGCGGCCG CTCGAGCAGA 
     3181 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3241 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3301 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3361 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3421 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3481 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3541 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3601 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     3661 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     3721 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     3781 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     3841 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     3901 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     3961 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4021 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4081 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4141 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4201 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4261 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4321 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4381 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4441 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4501 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4561 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4621 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     4681 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     4741 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     4801 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     4861 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     4921 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     4981 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5041 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5101 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5161 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5221 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5281 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5341 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5401 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5461 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5521 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     5581 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     5641 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     5701 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     5761 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     5821 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     5881 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     5941 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6001 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6061 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6121 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6181 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6241 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6301 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6361 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6421 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6481 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6541 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     6601 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     6661 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     6721 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     6781 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     6841 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     6901 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     6961 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//