LOCUS       MR226219                7068 bp    DNA     circular  SYN 
DEFINITION  Capn3 (Myc-DDK-tagged) - Mouse calpain 3 (Capn3), transcript variant c
ACCESSION   NM_001177799
VERSION     NM_001177799.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3225..3316 
                     /label="Myc-DDK"  
     misc_feature    3346..3931 
                     /label="PolyA signal"  
     misc_feature    3403..3422 
                     /label="Restriction Enzyme"  
     misc_feature    4207..4879 
                     /label="ColE1"  
     misc_feature    5309..6103 
                     /label="Kan/Neo"  
     misc_feature    6138..6440 
                     /label="SV40 ori"  
     misc_feature    6526..7003 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3207..3213 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3207 
                     /label="Capn3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAACTGTT ATTAGTCCAA CTGTGGCCCC AAGGACGGGG GCTGAGCCCA 
     1081 GGTCCCCAGG GCCAGTTCCT CACCCAGCTC AAGGCAAGAC CACCGAGGCT GGAGGTGGAC 
     1141 ACCCAAGTGG CATCTATTCG GCCATCATTA GCCGCAATTT TCCGATCATC GGTGTGAAAG 
     1201 AGAAGACATT TGAGCAGCTC CGCAGGAAGT GCCTAGAGAA GAAAGTTCTT TATCTAGACC 
     1261 CCGAGTTCCC ACCGGATGAG ACCTCTCTCT TTTACAGTCA GAAGTTCCCC ATCCAGTTCG 
     1321 TCTGGAAGAG ACCTCCGGAA ATTTGTGAGA ATCCCCGGTT TATCATTGGT GGAGCCAACA 
     1381 GGACTGACAT CTGCCAAGGA GATCTAGGGG ACTGCTGGTT TCTTGCAGCC ATCGCCTGCC 
     1441 TGACCCTGAA TGAGCGGCTG CTTTTCCGAG TTATACCCCA TGATCAAAGT TTCACTGAAA 
     1501 ACTACGCAGG GATCTTCCAC TTCCAGTTCT GGCGCTATGG AGACTGGGTA GATGTGGTCA 
     1561 TTGACGACTG TCTGCCAACA TACAACAATC AGCTGGTTTT CACCAAATCC AACCACCGCA 
     1621 ATGAGTTCTG GAGTGCTCTC CTTGAGAAGG CTTATGCCAA GCTCCATGGC TCCTATGAAG 
     1681 CTCTGAAAGG TGGGAATACC ACAGAAGCCA TGGAGGACTT CACAGGAGGG GTGACAGAGT 
     1741 TTTTTGAGAT CAAGGATGCT CCGAGTGACA TGTACAAGAT TATGAGGAAA GCTATCGAGA 
     1801 GAGGCTCCCT CATGGGCTGC TCCATTGACA CAATTGTTCC AGTGCAGTAT GAAACAAGAA 
     1861 TGGCCTGTGG GTTGGTGAAA GGGCATGCCT ATTCAGTCAC TGGGCTGGAG GAGGCCCTGT 
     1921 TCAAAGGTGA GAAGGTGAAG TTGGTGCGGC TGCGGAACCC CTGGGGCCAG GTGGAGTGGA 
     1981 ACGGCTCTTG GAGTGATGGT TGGAAGGACT GGAGCTTTGT AGACAAAGAT GAGAAGGCCC 
     2041 GTCTACAGCA CCAGGTTACT GAGGATGGAG AGTTCTGGAT GTCATATGAT GACTTCGTCT 
     2101 ACCATTTCAC GAAGCTGGAG ATCTGCAACC TCACAGCTGA CGCCCTGGAG TCCGATAAGC 
     2161 TTCAGACCTG GACGGTGTCT GTAAACGAGG GCCGCTGGGT GAGGGGCTGT TCTGCTGGAG 
     2221 GCTGCCGAAA CTTCCCAGAC ACTTTCTGGA CCAACCCGCA GTACCGTCTC AAGCTTCTGG 
     2281 AGGAAGACGA TGACCCTGAG GACTCTGAGG TAATCTGCAG CTTCCTCGTG GCTCTGATGC 
     2341 AGAAGAACCG GCGCAAGGAC CGGAAGCTGG GGGCCAACCT CTTCACTATT GGCTTCGCCA 
     2401 TCTACGAGGT TCCCAAAGAG ATGCACGGGA ATAAGCAACA CCTGCAGAAG GACTTCTTCT 
     2461 TGTACAACGC CTCCAAGGCC AGAAGCAAAA CCTACATCAA CATGCGGGAG GTGTCCCAGC 
     2521 GCTTCCGCCT GCCACCCAGC GAGTATGTCA TCGTACCCTC CACCTACGAG CCCCACCAGG 
     2581 AGGGGGAATT CATCCTCCGA GTCTTCTCCG AAAAGAGGAA TCTCTCTGAG GAAGCTGAAA 
     2641 ATACAATCTC TGTGGATCGG CCAGTGCCAA GGCCTGGCCA CACAGACCAG GAAAGTGAGG 
     2701 AGCAGCAGCA ATTCCGGAAC ATCTTCAGGC AGATCGCAGG CGACGACATG GAGATCTGTG 
     2761 CAGATGAACT CAAGAATGTC CTCAACACAG TGGTGAACAA ACACAAGGAC CTGAAGACAC 
     2821 AAGGGTTCAC GCTGGAGTCC TGCAGAAGCA TGATAGCTCT CATGGATACA GATGGCTCTG 
     2881 GAAGACTGAA TCTTCAAGAG TTCCATCACC TCTGGAAAAA GATCAAGGCC TGGCAGAAAA 
     2941 TCTTCAAGCA CTATGACACA GACCATTCCG GTACCATCAA TAGCTATGAG ATGCGAAATG 
     3001 CAGTCAATGA TGCAGGCTTC CATCTCAACA GCCAACTCTA TGACATCATC ACCATGCGCT 
     3061 ATGCGGACAA ACACATGAAC ATCGACTTTG ACAGCTTCAT CTGCTGCTTC GTCAGGCTGG 
     3121 AAGGGATGTT CAGAGCTTTT AACGCATTTG ACAAGGATGG CGATGGTATC ATCAAACTGA 
     3181 ATGTACTTGA GTGGCTGCAG CTTACCATGT ATGCCACGCG TACGCGGCCG CTCGAGCAGA 
     3241 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3301 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3361 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3421 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3481 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3541 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3601 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3661 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     3721 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     3781 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     3841 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     3901 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     3961 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4021 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4081 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4141 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4201 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4261 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4321 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4381 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4441 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4501 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4561 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4621 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4681 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     4741 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     4801 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     4861 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     4921 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     4981 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5041 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5101 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5161 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5221 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5281 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5341 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5401 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5461 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5521 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5581 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     5641 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     5701 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     5761 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     5821 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     5881 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     5941 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6001 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6061 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6121 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6181 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6241 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6301 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6361 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6421 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6481 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6541 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6601 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     6661 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     6721 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     6781 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     6841 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     6901 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     6961 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7021 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//