LOCUS       MR226201                7665 bp    DNA     circular  SYN 
DEFINITION  Gprc6a (Myc-DDK-tagged) - Mouse G protein-coupled receptor, family C, group 6, member A (Gprc6a)
ACCESSION   NM_153071
VERSION     NM_153071.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3822..3913 
                     /label="Myc-DDK"  
     misc_feature    3943..4528 
                     /label="PolyA signal"  
     misc_feature    4000..4019 
                     /label="Restriction Enzyme"  
     misc_feature    4804..5476 
                     /label="ColE1"  
     misc_feature    5906..6700 
                     /label="Kan/Neo"  
     misc_feature    6735..7037 
                     /label="SV40 ori"  
     misc_feature    7123..7600 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3804..3810 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3804 
                     /label="Gprc6a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCTATTG ATTACAGTAG TTACTTGCTT CATGATTATT CTTGATACCT 
     1081 CCCAGTCTTG TCATACCCCA GATGACTTCG TGGCTATCAC TTCTCCTGGA CATATCATGA 
     1141 TTGGTGGCTT GTTTGCCATT CATGAAAAAA TGTTGTCCTC AGATGACCAT CCCAGACGAC 
     1201 CACAAATCCA GAAATGTGCT GGCTTTGAAA TATCAGTGTT TCTTCAAACT CTGGCTATGA 
     1261 TACACAGCAT TGAGATGATC AATAATTCAA CACTGTTATC CGGAGTCAAG CTGGGATATG 
     1321 AAATCTATGA CACTTGTACC GAAGTCACAG CAGCAATGGC TGCCACTCTG AGGTTCCTCT 
     1381 CAAAATTCAA CTGCTCTAGA GAAACTGTGG TCTTTCAGTG TGACTATTCC AGCTACATGC 
     1441 CAAGGGTCAA GGCTGTCATA GGTGCTGGCT ACTCTGAAAC ATCCATCGCG GTCTCAAGGA 
     1501 TGCTGAACTT ACAGCTCATG CCACAGGTGA GTTATGAATC CACTGCAGAA ATCCTGAGTG 
     1561 ACAAGATCCG CTTTCCTTCA TTTTTACGGA CTGTGCCTAG TGACTTCTAC CAAACTAAAG 
     1621 CAATGGCCCA CCTGATCCGA CAATCGGGAT GGAACTGGAT TGGTGCCATA ACAACCGATG 
     1681 ATGATTATGG AAGACTAGCT CTCAACACAT TTGCAATACA GGCTGCTGAA AACAATGTGT 
     1741 GCATTGCCTT CAAAGAGGTT CTGCCAGCCT TCCTCTCAGA TAATACCATT GAAGTGAGAA 
     1801 TTAACCAGAC TCTGGAGAAG ATCATTGCAG AAGCCCAGGT TAATGTCATT GTGGTGTTTC 
     1861 TGAGAAAATT CCATGTTTTC AATCTCTTCA CGAAAGCCAT TGAAAGGAAA ATAAGTAAGA 
     1921 TCTGGATTGC TAGTGATAAT TGGTCAACCG CTACCAAGAT TATCACCATC CCTAATGTTA 
     1981 AGAAGCTTGG CAAAGTGGTG GGGTTTGCCT TTAGGAGAGG AAATACGTCT TCTTTCCATT 
     2041 CTTTTCTTCA AACTCTGCAT ATGTACCCCA ATGACAATAA CAAACCCCTA CATGAATTTG 
     2101 CCATGCTCGT TTCTGCCTGT AAATACATCA AAGACGGTGA TTTGAGCCAA TGCATATCCA 
     2161 ACTATTCTCA GGCAACTTTG ACCTATGACA CTACCAAGAC CATTGAAAAC CACTTATTCA 
     2221 AGAGAAATGA TTTCCTCTGG CATTATACTG AGCCGGGACT CATTTATAGC ATTCAGCTTG 
     2281 CAGTGTTCGC CCTTGGTCAT GCCATCCGGG ATCTGTGCCA AGCTCGAGAC TGCAAGAAAC 
     2341 CCAACGCCTT TCAGCCATGG GAGCTACTTG CCGTGCTGAA AAACGTGACA TTCACTGATG 
     2401 GAAGGAATTC ATTTCACTTT GATGCCCACG GGGATTTAAA TACTGGTTAT GACGTGGTGC 
     2461 TCTGGAAGGA GACCAATGGC CTCATGACTG TCACAAAGAT GGCAGAATAT GACCTGCAGC 
     2521 GTGACGTCTT CATCACCACA AACCAAGAAA CAAAACATGA ATTCAGGAAA CTTAAGCAAA 
     2581 TTCTATCTAA ATGCTCCAAG GAATGCAGCC CTGGTCAAAT GAAGAAAGCC ACAGGAAGCC 
     2641 AACACAGCTG TTGCTATGAA TGTGTGAGCT GCCCAGAAAA CCACTACAGT AATGAGACAG 
     2701 ATATGGATCA CTGCCTTTTA TGCAACAATG AAACCCACTG GGCCCCAGTA AGGAGCACTA 
     2761 CCTGCTTTGA AAAGGAAGTG GAGTATCTGG ACTGGGATGA CTCCTTGGCT CTCCTCCTCA 
     2821 TTGCCCTCTC CCTACTTGGA ATCGCCTTTG TTCTGGCCAT TGGCATAATA TTTACAAGAA 
     2881 ACCTGAAGAC ACCTGTCGTG AAATCATCTG GAGGATTAGT GGTCTGCTAC GTGATGCTCA 
     2941 TCTGCCACGC CCTTAACTTT GCCAGCACAG GCTTTTTTAT TGGAGAACCA CAAGACTTTG 
     3001 CGTGCAAGAC CAGGCAGACC TTATTTGGTG TGAGCTTTAC TCTCTGTGTC TCCTGTATTT 
     3061 TGACCAAGTC CCTGAAAATT TTGCTAGCTT TCAGTTTTGA CCCCAAGCTG ACAATGTTCC 
     3121 TGAAGTGCCT TTATAGGCCT GTTCCCATTG TTCTTACCTG CACGGGCATT CAAGTGGTCA 
     3181 TTTGCACTCT TTGGCTGGTG CTGGCAGCAC CTAGTGTGGA AGAGAATATC TCCTTGCCTA 
     3241 GAGTCATTAT CCTGGAATGT GAGGAGGGGT CAGCACTGGC ATTTGGTACC ATGCTGGGCT 
     3301 ACATCACAGT TCTGGCCTTC ATTTGCTTTG TATTTGCATT CAAGGGGAGG AAACTTCCTG 
     3361 AGAATTACAA CGAAGCCAAG TTCCTGACCT TTGGGATGCT GATTTACTTC ATAGCTTGGA 
     3421 TCACATTCAT CCCTGTCTAT ACTACCACAT TCGGCAAGTA TTTGCCCGCC GTGGAGATTA 
     3481 TAGTCATTCT GATATCCAAT TATGGGATTC TCTGCTGTAT ATTCTTCCCC AAGTGCTACA 
     3541 TTATTCTTTG CAAGCAGAAG ACTAACACCA AATCAGCCTT CCTCCAGATG GTTTACAACT 
     3601 ACTCTGCTCA CAGTGTGGAC AGCCTTGCCT TGAGTCATGT TTCCCTGGAC TCCACCAGCT 
     3661 ACGATACTGC AACAACCAAT CAGAGCCCTG GTAACAAGAT GACAGCCTGT CAGAACGACA 
     3721 ACCATCTTCC TGCACAAGTG CTCCCACACA CAGGCACGGC AAAGACTATC AAAGCGTCTA 
     3781 AAACTTTGCG TCAGAAAAGA AGTTCAAGTA TAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3841 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3901 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3961 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4021 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4081 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4141 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4201 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4261 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4321 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4381 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4441 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4501 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4561 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4621 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4681 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4741 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4801 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4861 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4921 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4981 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5041 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5101 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5161 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5221 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5281 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5341 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5401 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5461 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5521 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5581 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5641 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5701 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5761 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5821 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5881 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5941 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6001 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6061 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6121 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6181 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6241 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6301 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6361 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6421 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6481 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6541 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6601 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6661 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6721 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6781 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6841 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6901 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6961 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7021 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7081 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7141 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7201 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7261 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7321 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7381 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7441 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7501 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7561 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7621 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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