LOCUS       MR226175                8079 bp    DNA     circular  SYN 
DEFINITION  Pum2 (Myc-DDK-tagged) - Mouse pumilio 2 (Drosophila) (Pum2), transcript variant 2
ACCESSION   NM_030723
VERSION     NM_030723.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4236..4327 
                     /label="Myc-DDK"  
     misc_feature    4357..4942 
                     /label="PolyA signal"  
     misc_feature    4414..4433 
                     /label="Restriction Enzyme"  
     misc_feature    5218..5890 
                     /label="ColE1"  
     misc_feature    6320..7114 
                     /label="Kan/Neo"  
     misc_feature    7149..7451 
                     /label="SV40 ori"  
     misc_feature    7537..8014 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4218..4224 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4218 
                     /label="Pum2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATCATGAT TTTCAAGCTC TTGCATTAGA ATCTCGGGGA ATGGGAGAGC 
     1081 TTTTGCCTAC CAAAAAGTTT TGGGAACCTG ATGATTCAAC AAAAGATGGA CAAAAAGGCA 
     1141 TATTTCTTGG GGATGATGAA TGGAGAGAGA CTGCATGGGG AACTTCTCAT CATTCAATGT 
     1201 CCCAGCCTAT TATGGTGCAG AGAAGATCTG GACAGAGTTT TCATGGAAAC AGTGAAGTAA 
     1261 ATGCAATTCT TTCTCCACGC TCAGAAAGTG GAGGCCTTGG TGTGAGCATG GTAGAATATG 
     1321 TATTAAGTTC CTCTCCTGCT GATAAATTGG ATTCTCGATT TAGGAAGGGA ACTTTTGGTA 
     1381 CTAGAGATGC TGAAACAGAT GGACCTGAGA AAGGAGATCA AAAAGGCAAG GCTTCTCCAT 
     1441 TTGAGGAGGA CCAAAACAGA GATCTTAAAC AAGATGATGA GGACTCTAAA ATAAATGGCA 
     1501 GAGGTTTGCC AAATGGAATG GATGCCGATT GCAAAGATTT TAATCGCACT CCTGGAAGTC 
     1561 GCCAAGCCTC TCCAACTGAA GTAGTTGAGC GCCTTGGCCC TAGTACTAAT CCCCCAGAAG 
     1621 GATTGGGCCC TCTTCCTAAT CCGACAGCGA ATAAACCACT TGTTGAAGAA TTTTCAAACC 
     1681 CTGAAACTCA GAATCTGGAT GCAATGGACC AAGTTGGTCT GGATTCCTTA CAGTTTGACT 
     1741 ATCCTGGTAA TCAGGTACCC ATGGATTCTT CAGGAGCTAC TGTAGGCCTT TTTGACTACA 
     1801 ATTCCCAACA GCAGCTCTTT CAGAGGACTA GTGCACTAAC AGTTCAGCAG TTAACTGCAG 
     1861 CTCAGCAGCA GCAGTATGCA TTAGCAGCAG CTCAGCAGCC ACATATAGCT GGTGTATTCT 
     1921 CAGCAGGCCT TGCTCCAGCT GCATTTGTGC CAAATCCATA TATTATTAGT GCTGCTCCTC 
     1981 CAGGGACTGA CCCGTATACT GCAGCAGGAT TGGCTGCAGC AGCAACATTA GCAGGTCCAG 
     2041 CAGTGGTTCC ACCTCAGTAT TACGGTGTTC CATGGGGAGT GTATCCAGCC AATTTATTTC 
     2101 AGCAACAAGC TGCAGCTGCG GCAAGCAACA CAGCCAACCA GCAAGCAGCA TCACAAGCTC 
     2161 AGCCTGGACA GCAGCAGGTT CTTCGTCCTG GAGCAGGTCA GCGTCCTATT ACTCCAAGTC 
     2221 AGGGCCAACA AGGGCAGCAA GCAGAGTCAC TTGCAGCAGC TGCAAACCCA ACTTTGGCTT 
     2281 TTGGTCAGAG TCTTGCTGCA GGCATGCCAG GCTATCAAGT ACTAGCTCCA ACTGCCTATT 
     2341 ATGATCAGAC TGGTGCCTTA GTGGTTGGCC CCGGAGCAAG AACTGGCCTT GGAGCTCCAG 
     2401 TACGATTAAT GGCTCCAACA CCTGTCTTAA TAAGTTCAAC AGCAGCACAA GCTGCAGCAG 
     2461 CAGCAGCAGC AGCTGGAGGA ACTGCAAATA GTCTTACAGG CAGCACAAAT GGTCTGTTTC 
     2521 GGCCAATTGG CACTCAGCCA CCACAGCAGC AGCAGCAGCA GCAGCAACCA AGCACTAACC 
     2581 TGCAGTCTAA TTCATTTTAT GGGAGCAGCT CTTTGACTAA CAGCTCCCAG AGCAGCTCTT 
     2641 TATTCTCTCA TGGACCTGGC CAACCTGGAA GTGCGTCTCT TGGCTTTGGA AGTGGTAGCT 
     2701 CTTTAGGAGC TGCTATAGGC TCAGCTCTCA GTGGATTTGG CTCATCAGTT GGCAGTTCTG 
     2761 CAAGTAGTAG TGCCACAAGG AGAGAGTCTC TATCTACTAG CTCTGACTTG TACAAAAGAT 
     2821 CTAGTAGCAG CCTAGCACCC ATAGGGCAAC CATTTTACAA TAGTCTGGGA TTTTCCTCCT 
     2881 CTCCAAGTCC AATAGGCATG CCTCTGCCAA GCCAAACTCC AGGACATTCA CTTACGCCAC 
     2941 CGCCATCACT TTCATCACAT GGATCCTCAT CCAGTTTGCA TTTAGGAGGA CTGACAAACG 
     3001 GTAGTGGTCG GTATATCTCT GCAGCACCTG GAGCAGAAGC AAAGTACCGA AGTGCTTCAA 
     3061 GCACTTCCAG TCTATTTAGC TCCAGCAGCC AGCTCTTTCC TCCTTCTCGG CTCCGCTATA 
     3121 ATAGATCTGA CATCATGCCC TCCGGCCGCA GTAGGTTATT GGAAGATTTC AGGAACAACC 
     3181 GCTTCCCCAA CCTTCAGCTC AGAGACTTAA TTGGACATAT AGTCGAGTTT TCTCAAGACC 
     3241 AGCATGGTTC CAGATTCATA CAGCAAAAGC TAGAGAGAGC TACTCCAGCT GAGCGACAAA 
     3301 TAGTATTTAA TGAAATTCTA CAGGCAGCCT ATCAATTAAT GACAGATGTT TTTGGAAACT 
     3361 ATGTTATACA GAAGTTTTTT GAGTTTGGAA GTTTGGATCA GAAATTAGCC CTGGCTACTC 
     3421 GTATTCGTGG TCATGTTCTA CCATTAGCCT TGCAGATGTA TGGCTGCCGT GTTATTCAAA 
     3481 AGGCGTTAGA ATCTATTTCT TCTGATCAGC AGGTAATTAG TGAAATGGTT AAGGAACTAG 
     3541 ATGGCCATGT ACTTAAATGT GTGAAAGATC AAAATGGAAA CCATGTTGTA CAGAAATGCA 
     3601 TTGAATGTGT TCAGCCACAG TCACTGCAGT TCATCATCGA TGCTTTCAAA GGACAAGTAT 
     3661 TTGTGCTTTC AACCCATCCT TATGGCTGCA GAGTCATTCA GCGTATCTTA GAGCACTGCA 
     3721 CGGCAGAGCA GACCTTACCC ATCTTAGAAG AACTTCACCA ACACACAGAA CAGTTGGTAC 
     3781 AGGATCAGTA TGGCAATTAT GTTATTCAGC ATGTACTGGA ACATGGTCGA CCTGAAGACA 
     3841 AGAGCAAAAT TGTTTCCGAA ATCAGAGGAA AGGTCTTAGC CCTGAGTCAA CACAAATTTG 
     3901 CCAGCAATGT AGTAGAAAAG TGTGTTACTC ATGCCTCCCG TGCTGAGAGA GCTTTACTGA 
     3961 TTGATGAGGT CTGCTGTCAG AATGATGGTC CTCACAGTGC CTTATACACC ATGATGAAGG 
     4021 ATCAGTATGC CAACTATGTG GTTCAGAAGA TGATTGATAT GGCTGAGCCT GCGCAGAGAA 
     4081 AGATAATCAT GCACAAGATT CGACCTCATA TTACTACTCT TCGCAAATAC ACATATGGGA 
     4141 AGCATATACT GGCCAAGTTG GAAAAATACT ATCTGAAAAA CAGCCCAGAT CTAGGGCCAA 
     4201 TTGGAGGACC ACCAAATGGG ATGCTGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4261 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4321 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4381 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4441 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4501 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4561 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4621 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4681 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4741 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4801 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4861 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4921 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4981 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5041 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5101 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5161 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5221 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5281 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5341 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5401 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5461 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5521 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5581 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5641 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5701 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5761 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5821 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5881 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5941 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6001 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6061 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6121 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6181 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6241 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6301 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6361 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6421 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6481 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6541 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6601 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6661 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6721 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6781 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6841 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6901 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6961 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7021 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7081 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7141 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7201 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7261 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7321 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7381 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7441 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7501 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7561 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7621 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7681 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7741 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7801 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7861 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7921 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7981 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8041 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//