LOCUS       MR226164                8361 bp    DNA     circular  SYN 
DEFINITION  Itgal (Myc-DDK-tagged) - Mouse integrin alpha L (Itgal)
ACCESSION   NM_008400
VERSION     NM_008400.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4518..4609 
                     /label="Myc-DDK"  
     misc_feature    4639..5224 
                     /label="PolyA signal"  
     misc_feature    4696..4715 
                     /label="Restriction Enzyme"  
     misc_feature    5500..6172 
                     /label="ColE1"  
     misc_feature    6602..7396 
                     /label="Kan/Neo"  
     misc_feature    7431..7733 
                     /label="SV40 ori"  
     misc_feature    7819..8296 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4500..4506 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4500 
                     /label="Itgal"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTTCCGG ATTGCGGGCC CCAGACTTTT GCTACTGGGA CTCCAGCTGT 
     1081 TTGCCAAGGC CTGGAGCTAC AACCTGGACA CACGGCCTAC GCAGAGCTTC TTGGCACAAG 
     1141 CTGGAAGACA TTTTGGGTAC CAGGTCTTGC AGATTGAAGA TGGGGTTGTC GTGGGAGCCC 
     1201 CAGGTGAGGG GGACAACACG GGAGGCCTCT ATCACTGCCG AACAAGCAGC GAGTTCTGCC 
     1261 AGCCAGTCAG CCTACATGGT TCTAACCATA CCTCCAAGTA CTTGGGAATG ACGCTGGCAA 
     1321 CAGATGCCGC CAAGGGAAGC CTTTTGGCCT GTGACCCTGG ACTGTCTCGG ACATGCGATC 
     1381 AGAACACTTA CCTCAGTGGC CTCTGCTACC TCTTCCCCCA GAGTCTGGAG GGACCTATGT 
     1441 TACAAAATCG TCCCGCCTAT CAGGAATGTA TGAAGGGCAA AGTCGACCTG GTGTTTCTGT 
     1501 TCGATGGCTC ACAGAGCTTG GATAGAAAGG ACTTTGAAAA AATCCTGGAA TTCATGAAGG 
     1561 ATGTGATGAG GAAGCTCAGC AATACTTCCT ACCAGTTTGC TGCCGTCCAG TTCTCCACAG 
     1621 ACTGCAGAAC AGAATTTACT TTCTTGGACT ACGTTAAGCA GAACAAGAAC CCCGATGTTC 
     1681 TGCTAGGCAG CGTGCAGCCC ATGTTCTTGC TGACCAATAC CTTTCGTGCC ATCAACTATG 
     1741 TGGTGGCACA CGTGTTCAAA GAAGAGTCTG GTGCCAGGCC GGATGCTACC AAGGTGCTTG 
     1801 TCATCATTAC AGACGGGGAG GCAAGTGATA AAGGCAACAT CAGTGCGGCC CACGACATAA 
     1861 CCCGCTACAT CATCGGGATT GGCAAGCATT TTGTGAGCGT ACAAAAGCAA AAGACGCTCC 
     1921 ACATATTTGC CTCAGAACCT GTAGAGGAAT TTGTGAAGAT TCTGGACACC TTTGAGAAGC 
     1981 TGAAGGATCT TTTTACTGAC CTGCAGAGGA GGATTTATGC TATTGAGGGC ACAAACAGAC 
     2041 AGGACCTGAC ATCCTTTAAC ATGGAACTCT CCTCCAGCGG GATCAGCGCA GACCTCAGCA 
     2101 AGGGCCATGC AGTTGTGGGA GCTGTTGGGG CTAAGGATTG GGCCGGGGGC TTTCTGGACC 
     2161 TGCGTGAAGA CCTGCAGGGT GCCACATTTG TTGGGCAGGA ACCGCTGACC TCAGATGTGA 
     2221 GAGGGGGCTA CCTGGGTTAC ACTGTGGCCT GGATGACCTC CCGGAGCTCC AGACCCCTGC 
     2281 TGGCAGCAGG AGCCCCACGG TACCAGCATG TGGGACAAGT ACTGCTTTTC CAAGCCCCAG 
     2341 AGGCTGGAGG ACGTTGGAAC CAAACCCAGA AGATAGAAGG GACTCAGATC GGATCTTACT 
     2401 TTGGTGGGGA ACTATGTAGT GTTGACCTGG ACCAAGATGG CGAGGCAGAG CTGCTGCTGA 
     2461 TTGGAGCACC CCTGTTCTTT GGGGAGCAGA GAGGAGGCCG AGTGTTCACT TACCAGAGAA 
     2521 GACAGTCGCT GTTTGAAATG GTCTCAGAGC TACAGGGTGA CCCTGGCTAC CCGCTTGGTC 
     2581 GGTTTGGAGC CGCCATAACT GCCCTGACGG ACATCAATGG GGATAGGCTG ACTGATGTGG 
     2641 CTGTGGGAGC CCCTTTGGAG GAGCAGGGGG CTGTGTACAT CTTCAATGGG AAGCCTGGTG 
     2701 GGCTCAGTCC CCAGCCAAGC CAGCGTATAC AAGGAGCCCA GGTGTTCCCA GGAATCCGGT 
     2761 GGTTTGGCCG CTCCATCCAT GGGGTGAAGG ACCTTGGAGG GGACAGGCTG GCAGATGTGG 
     2821 TTGTAGGAGC TGAGGGTCGG GTGGTTGTGC TGAGCTCCAG GCCGGTGGTG GATGTGGTCA 
     2881 CTGAGCTGTC GTTCTCCCCA GAGGAAATCC CAGTGCACGA GGTGGAGTGC TCCTACTCAG 
     2941 CCAGGGAGGA GCAGAAACAC GGAGTCAAGC TCAAGGCATG CTTCCGGATC AAGCCCCTCA 
     3001 CGCCACAGTT TCAAGGTCGC CTGCTTGCCA ACCTCAGCTA CACCCTGCAG CTGGATGGCC 
     3061 ATCGGATGAG GAGCCGAGGG TTGTTCCCAG ATGGAAGCCA CGAGCTCAGT GGAAACACCT 
     3121 CCATCACCCC AGATAAATCC TGCTTGGACT TCCACTTCCA CTTCCCGATC TGCATTCAAG 
     3181 ACCTCATCTC CCCTATCAAT GTCTCCCTGA ATTTCTCTCT TTTGGAGGAA GAAGGAACAC 
     3241 CAAGGGACCA AAAGGGCAGG GCCATGCAGC CTATCCTGAG ACCTTCAATC CACACAGTGA 
     3301 CTAAGGAGAT CCCTTTTGAG AAGAACTGTG GTGAAGATAA GAAGTGTGAG GCAAACCTGA 
     3361 CCCTGTCATC CCCTGCCAGA TCTGGACCCC TGCGTCTGAT GTCCTCTGCC AGCCTTGCTG 
     3421 TGGAGTGGAC ACTGAGCAAC TCAGGGGAAG ATGCCTACTG GGTGCGATTA GACCTGGACT 
     3481 TCCCTCGGGG ACTCTCCTTC CGGAAAGTGG AGATGCTTCA GCCACACAGC CGAATGCCTG 
     3541 TGAGCTGCGA GGAGCTCACC GAGGGGTCAA GTCTCCTGAC TAAGACACTG AAATGCAATG 
     3601 TAAGCTCTCC CATCTTCAAA GCAGGCCAGG AGGTGAGCCT CCAGGTGATG TTTAACACGC 
     3661 TACTCAACAG CTCCTGGGAA GACTTCGTTG AGCTGAATGG CACTGTGCAC TGTGAGAATG 
     3721 AGAACTCAAG CCTCCAGGAG GACAACTCAG CCGCCACCCA CATTCCTGTC CTGTACCCTG 
     3781 TCAACATCCT TACTAAGGAG CAGGAGAACT CCACTCTCTA TATCAGTTTC ACCCCTAAAG 
     3841 GTCCCAAGAC CCAACAAGTC CAGCATGTCT ACCAGGTGAG GATTCAGCCA TCTGCCTATG 
     3901 ACCACAACAT GCCCACACTA GAGGCCTTGG TTGGGGTGCC CTGGCCTCAC AGTGAGGACC 
     3961 CCATCACATA CACATGGAGT GTACAGACGG ATCCCCTTGT CACTTGCCAC AGCGAGGACC 
     4021 TGAAGAGGCC GTCCAGCGAA GCTGAGCCTT GTCTGCCTGG AGTCCAGTTC CGCTGTCCAA 
     4081 TTGTCTTCAG GCGGGAGATC CTCATCCAAG TGACGGGGAC CGTGGAACTC TCCAAGGAAA 
     4141 TCAAGGCCTC CTCCACACTC AGCCTCTGCA GCTCACTCTC CGTCTCCTTC AACAGCAGCA 
     4201 AGCATTTCCA TTTGTATGGC AGCAAAGCCT CCGAGGCCCA GGTCCTCGTG AAGGTTGACC 
     4261 TGATCCACGA GAAGGAGATG CTTCACGTGT ACGTACTCAG CGGCATTGGG GGCCTCGTGC 
     4321 TTCTGTTCCT GATTTTCCTG GCGCTCTACA AGGTTGGCTT CTTCAAACGG AACCTGAAGG 
     4381 AGAAGATGGA GGCTGATGGA GGTGTTCCAA ATGGAAGCCC TCCAGAAGAC ACTGACCCTC 
     4441 TGGCAGTACC TGGGGAAGAG ACCAAAGATA TGGGCTGTCT AGAGCCCCTC CGGGAGAGTG 
     4501 ACAAGGACAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4561 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4621 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4681 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4741 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4801 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4861 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4921 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4981 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5041 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5101 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5161 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5221 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5281 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5341 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5401 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5461 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5521 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5581 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5641 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5701 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5761 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5821 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5881 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5941 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6001 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6061 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6121 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6181 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6241 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6301 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6361 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6421 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6481 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6541 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6601 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6661 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6721 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6781 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6841 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6901 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6961 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7021 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7081 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7141 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7201 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7261 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7321 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7381 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7441 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7501 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7561 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7621 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7681 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7741 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7801 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7861 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7921 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7981 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8041 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8101 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8161 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8221 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8281 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8341 CAAAAATTTA ACGCGAATTT T
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