LOCUS       MR226160                8511 bp    DNA     circular  SYN 
DEFINITION  Egfr (Myc-DDK-tagged) - Mouse epidermal growth factor receptor (Egfr), transcript variant 1
ACCESSION   NM_207655
VERSION     NM_207655.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4668..4759 
                     /label="Myc-DDK"  
     misc_feature    4789..5374 
                     /label="PolyA signal"  
     misc_feature    4846..4865 
                     /label="Restriction Enzyme"  
     misc_feature    5650..6322 
                     /label="ColE1"  
     misc_feature    6752..7546 
                     /label="Kan/Neo"  
     misc_feature    7581..7883 
                     /label="SV40 ori"  
     misc_feature    7969..8446 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4650..4656 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4650 
                     /label="Egfr"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGACCCTCA GGGACCGCGA GAACCACACT GCTGGTGTTG CTGACCGCGC 
     1081 TCTGCGCCGC AGGTGGGGCG TTGGAGGAAA AGAAAGTCTG CCAAGGCACA AGTAACAGGC 
     1141 TCACCCAACT GGGCACTTTT GAAGACCACT TTCTGAGCCT GCAGAGGATG TACAACAACT 
     1201 GTGAAGTGGT CCTTGGGAAC TTGGAAATTA CCTATGTGCA AAGGAATTAC GACCTTTCCT 
     1261 TCTTAAAGAC CATCCAGGAG GTGGCCGGCT ATGTCCTCAT TGCCCTCAAC ACCGTGGAGA 
     1321 GAATCCCTTT GGAGAACCTG CAGATCATCA GGGGAAATGC TCTTTATGAA AACACCTATG 
     1381 CCTTAGCCAT CCTGTCCAAC TATGGGACAA ACAGAACTGG GCTTAGGGAA CTGCCCATGC 
     1441 GGAACTTACA GGAAATCCTG ATTGGTGCTG TGCGATTCAG CAACAACCCC ATCCTCTGCA 
     1501 ATATGGATAC TATCCAGTGG AGGGACATCG TCCAAAACGT CTTTATGAGC AACATGTCAA 
     1561 TGGACTTACA GAGCCATCCG AGCAGTTGCC CCAAATGTGA TCCAAGCTGT CCCAATGGAA 
     1621 GCTGCTGGGG AGGAGGAGAG GAGAACTGCC AGAAATTGAC CAAAATCATC TGTGCCCAGC 
     1681 AATGTTCCCA TCGCTGTCGT GGCAGGTCCC CCAGTGACTG CTGCCACAAC CAATGTGCTG 
     1741 CGGGGTGTAC AGGGCCCCGA GAGAGTGACT GTCTGGTCTG CCAAAAGTTC CAAGATGAGG 
     1801 CCACATGCAA AGACACCTGC CCACCACTCA TGCTGTACAA CCCCACCACC TATCAGATGG 
     1861 ATGTCAACCC TGAAGGGAAG TACAGCTTTG GTGCCACCTG TGTGAAGAAG TGCCCCCGAA 
     1921 ACTACGTGGT GACAGATCAT GGCTCATGTG TCCGAGCCTG TGGGCCTGAC TACTACGAAG 
     1981 TGGAAGAAGA TGGCATCCGC AAGTGTAAAA AATGTGATGG GCCCTGTCGC AAAGTTTGTA 
     2041 ATGGCATAGG CATTGGTGAA TTTAAAGACA CACTCTCCAT AAATGCTACA AACATCAAAC 
     2101 ACTTCAAATA CTGCACTGCC ATCAGCGGGG ACCTTCACAT CCTGCCAGTG GCCTTTAAGG 
     2161 GGGATTCTTT CACGCGCACT CCTCCTCTAG ACCCACGAGA ACTAGAAATT CTAAAAACCG 
     2221 TAAAGGAAAT AACAGGCTTT TTGCTGATTC AGGCTTGGCC TGATAACTGG ACTGACCTCC 
     2281 ATGCTTTCGA GAACCTAGAA ATAATACGTG GCAGAACAAA GCAACATGGT CAGTTTTCTT 
     2341 TGGCGGTCGT TGGCCTGAAC ATCACATCAC TGGGGCTGCG TTCCCTCAAG GAGATCAGTG 
     2401 ATGGGGATGT GATCATTTCT GGAAACCGAA ATTTGTGCTA CGCAAACACA ATAAACTGGA 
     2461 AAAAACTCTT CGGGACACCC AATCAGAAAA CCAAAATCAT GAACAACAGA GCTGAGAAAG 
     2521 ACTGCAAGGC CGTGAACCAC GTCTGCAATC CTTTATGCTC CTCGGAAGGC TGCTGGGGCC 
     2581 CTGAGCCCAG GGACTGTGTC TCCTGCCAGA ATGTGAGCAG AGGCAGGGAG TGCGTGGAGA 
     2641 AATGCAACAT CCTGGAGGGG GAACCAAGGG AGTTTGTGGA AAATTCTGAA TGCATCCAGT 
     2701 GCCATCCAGA ATGTCTGCCC CAGGCCATGA ACATCACCTG TACAGGCAGG GGACCAGACA 
     2761 ACTGCATCCA GTGTGCCCAC TACATTGATG GCCCACACTG TGTCAAGACC TGCCCAGCTG 
     2821 GCATCATGGG AGAGAACAAC ACTCTGGTCT GGAAGTATGC AGATGCCAAT AATGTCTGCC 
     2881 ACCTATGCCA CGCCAACTGT ACCTATGGAT GTGCTGGGCC AGGTCTTCAA GGATGTGAAG 
     2941 TGTGGCCATC TGGGCCAAAG ATACCATCTA TTGCCACTGG GATTGTGGGT GGCCTCCTCT 
     3001 TCATAGTGGT GGTGGCCCTT GGGATTGGCC TATTCATGCG AAGACGTCAC ATTGTTCGAA 
     3061 AGCGTACACT ACGCCGCCTG CTTCAAGAGA GAGAGCTCGT GGAACCTCTC ACACCCAGCG 
     3121 GAGAAGCTCC AAACCAAGCC CACTTGAGGA TATTAAAGGA AACAGAATTC AAAAAGATCA 
     3181 AAGTTCTGGG TTCGGGAGCA TTTGGCACAG TGTATAAGGG TCTCTGGATC CCAGAAGGTG 
     3241 AGAAAGTAAA AATCCCGGTG GCCATCAAGG AGTTAAGAGA AGCCACATCT CCAAAAGCCA 
     3301 ACAAAGAAAT CCTTGACGAA GCCTATGTGA TGGCTAGTGT GGACAACCCT CATGTATGCC 
     3361 GCCTCCTGGG CATCTGTCTG ACCTCCACTG TCCAGCTCAT TACACAGCTC ATGCCCTACG 
     3421 GTTGCCTCCT GGACTACGTC CGAGAACACA AGGACAACAT TGGCTCCCAG TACCTCCTCA 
     3481 ACTGGTGTGT GCAGATTGCA AAGGGCATGA ACTACCTGGA AGATCGGCGT TTGGTGCACC 
     3541 GTGACTTGGC AGCCAGGAAT GTACTGGTGA AGACACCACA GCATGTCAAG ATCACAGATT 
     3601 TTGGGCTGGC CAAACTGCTT GGTGCTGAAG AGAAAGAATA TCATGCCGAG GGGGGCAAAG 
     3661 TGCCTATCAA GTGGATGGCT TTGGAATCAA TTTTACACCG AATTTATACA CACCAAAGTG 
     3721 ATGTCTGGAG CTATGGTGTC ACTGTGTGGG AACTGATGAC CTTTGGGTCC AAGCCTTATG 
     3781 ATGGAATCCC AGCAAGTGAC ATCTCATCCA TCCTAGAGAA AGGAGAGCGC CTTCCACAGC 
     3841 CACCTATCTG CACCATCGAT GTCTACATGA TCATGGTCAA GTGCTGGATG ATAGATGCTG 
     3901 ATAGCCGCCC AAAGTTCCGA GAGTTGATTC TTGAATTCTC CAAAATGGCC CGAGACCCAC 
     3961 AGCGCTACCT TGTTATCCAG GGGGATGAAA GAATGCATTT GCCAAGCCCT ACAGACTCCA 
     4021 ACTTTTACCG AGCCCTGATG GATGAAGAGG ACATGGAGGA TGTAGTTGAT GCTGATGAGT 
     4081 ATCTTATCCC ACAGCAAGGC TTCTTCAACA GCCCGTCCAC GTCGAGGACT CCCCTCTTGA 
     4141 GTTCTCTGAG TGCAACTAGC AACAATTCCA CTGTGGCTTG CATTAATAGA AATGGGAGCT 
     4201 GCCGTGTCAA AGAAGACGCC TTCTTGCAGC GGTACAGCTC CGACCCCACA GGTGCTGTAA 
     4261 CAGAGGACAA CATAGATGAC GCATTCCTCC CTGTACCTGA ATATGTAAAC CAATCTGTTC 
     4321 CCAAGAGGCC AGCAGGCTCT GTGCAGAACC CTGTCTATCA CAATCAGCCC CTGCATCCAG 
     4381 CTCCTGGAAG AGACCTGCAT TATCAAAATC CCCACAGCAA TGCAGTGGGC AACCCTGAGT 
     4441 ATCTCAACAC TGCCCAGCCT ACCTGTCTCA GTAGTGGGTT TAACAGCCCT GCACTCTGGA 
     4501 TCCAGAAAGG CAGTCACCAA ATGAGCCTAG ACAACCCTGA CTACCAGCAG GACTTCTTCC 
     4561 CCAAGGAAAC CAAGCCAAAT GGCATATTTA AGGGCCCCAC AGCTGAAAAT GCAGAGTACC 
     4621 TACGGGTGGC ACCTCCAAGC AGTGAGTTTA TTGGAGCAAC GCGTACGCGG CCGCTCGAGC 
     4681 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4741 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4801 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4861 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4921 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4981 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5041 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5101 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5161 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5221 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5281 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5341 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5401 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5461 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5521 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5581 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5641 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5701 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5761 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5821 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5881 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5941 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6001 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6061 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6121 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6181 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6241 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6301 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6361 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6421 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6481 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6541 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6601 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6661 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6721 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6781 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6841 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6901 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6961 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7021 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7081 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7141 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7201 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7261 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7321 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7381 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7441 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7501 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7561 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7621 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7681 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7741 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7801 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7861 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7921 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7981 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8041 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8101 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8161 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8221 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8281 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8341 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8401 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8461 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//