LOCUS       MR226148                8574 bp    DNA     circular  SYN 
DEFINITION  Irs1 (Myc-DDK-tagged) - Mouse insulin receptor substrate 1 (Irs1)
ACCESSION   NM_010570
VERSION     NM_010570.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4731..4822 
                     /label="Myc-DDK"  
     misc_feature    4852..5437 
                     /label="PolyA signal"  
     misc_feature    4909..4928 
                     /label="Restriction Enzyme"  
     misc_feature    5713..6385 
                     /label="ColE1"  
     misc_feature    6815..7609 
                     /label="Kan/Neo"  
     misc_feature    7644..7946 
                     /label="SV40 ori"  
     misc_feature    8032..8509 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4713..4719 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4713 
                     /label="Irs1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGCCCT CCGGATACCG ATGGCTTCTC AGACGTGCGC AAGGTGGGCT 
     1081 ACCTGCGCAA GCCCAAGAGT ATGCATAAGC GCTTTTTCGT GCTGCGGGCG GCCAGCGAGG 
     1141 CCGGGGGCCC AGCGCGCCTG GAGTATTATG AGAACGAGAA GAAGTGGCGG CACAAGTCGA 
     1201 GCGCCCCCAA ACGCTCGATC CCCCTCGAGA GCTGTTTCAA CATCAACAAG CGGGCTGACT 
     1261 CCAAGAACAA GCACCTGGTG GCTCTCTACA CCCGAGACGA ACACTTTGCC ATTGCCGCGG 
     1321 ATAGCGAGGC TGAGCAAGAC AGCTGGTACC AGGCTCTTCT GCAGCTGCAT AATCGGGCAA 
     1381 AGGCCCACCA TGACGGGGCT GGAGGAGGCT GCGGCGGTAG CTGCAGCGGC AGCTCTGGCG 
     1441 TTGGAGAGGC AGGGGAGGAC TTGAGCTATG ACACGGGTCC AGGACCCGCG TTCAAGGAGG 
     1501 TCTGGCAGGT TATCCTGAAA CCCAAGGGCC TGGGTCAGAC AAAGAACCTG ATTGGCATCT 
     1561 ACCGCCTCTG CCTGACCAGC AAGACCATCA GCTTTGTGAA GCTGAACTCC GAGGCAGCCG 
     1621 CTGTGGTGCT GCAGCTGATG AATATCAGAC GCTGTGGCCA CTCAGAGAAC TTCTTCTTCA 
     1681 TCGAGGTGGG GCGTTCCGCT GTGACAGGGC CTGGCGAGTT CTGGATGCAA GTGGATGACT 
     1741 CCGTGGTGGC CCAGAACATG CATGAGACCA TTCTAGAGGC AATGAGGGCC ATGAGCGATG 
     1801 AGTTTCGCCC TCGCAGCAAA AGCCAGTCTT CATCCAGTTG CTCCAACCCC ATCAGCGTTC 
     1861 CCCTGCGCAG GCACCATCTC AACAACCCTC CACCCAGCCA GGTGGGACTG ACTCGGAGAT 
     1921 CTCGAACTGA GAGCATCACT GCCACCTCCC CTGCCAGTAT GGTGGGTGGG AAACCAGGTT 
     1981 CCTTCCGGGT GCGTGCCTCC AGCGATGGCG AAGGCACCAT GTCCCGTCCA GCATCAGTGG 
     2041 ATGGCAGTCC TGTGAGCCCT AGCACCAACA GGACCCACGC CCATCGGCAT CGAGGCAGCT 
     2101 CCAGGCTGCA CCCCCCACTC AACCACAGCC GCTCCATCCC CATGCCTTCT TCTAGATGCT 
     2161 CACCTTCAGC CACCAGCCCA GTGAGTCTGT CATCTAGTAG TACCAGTGGC CATGGCTCCA 
     2221 CTTCAGACTG TCTCTTCCCG AGGCGCTCTA GTGCTTCCGT GTCCGGTTCG CCTAGCGATG 
     2281 GCGGTTTCAT CTCTTCTGAT GAGTATGGTT CTAGTCCCTG CGATTTCCGA AGTTCCTTCC 
     2341 GCAGTGTCAC CCCAGATTCC CTGGGGCACA CCCCACCAGC CAGGGGTGAG GAGGAGCTGA 
     2401 GCAATTATAT CTGCATGGGT GGCAAGGGAG CCTCCACCTT GGCTGCTCCC AATGGCCACT 
     2461 ACATTTTGTC TAGGGGTGGC AACGGCCATC GCTACATCCC AGGTGCTAAC TTGGGGACAA 
     2521 GCCCAGCGCT GCCTGGAGAT GAAGCCGCGG GTGCAGCAGA TCTGGATAAC CGGTTTCGAA 
     2581 AGAGAACTCA CTCCGCAGGC ACATCTCCTA CCATTTCCCA TCAGAAGACC CCCTCACAGT 
     2641 CTTCAGTGGC TTCTATTGAG GAATATACAG AGATGATGCC CGCTGCCTAC CCACCAGGAG 
     2701 GTGGCAGTGG AGGCCGACTG CCCGGCTACC GGCATTCCGC CTTCGTGCCC ACCCACTCCT 
     2761 ATCCCGAAGA GGGTCTAGAG ATGCACCACT TGGAACGTCG TGGAGGCCAC CACCGTCCAG 
     2821 ACACCTCCAA CCTCCACACT GATGATGGCT ATATGCCCAT GTCTCCTGGG GTGGCTCCAG 
     2881 TGCCCAGCAA CCGCAAAGGA AATGGGGACT ATATGCCCAT GAGCCCCAAG AGTGTATCTG 
     2941 CCCCACAGCA GATCATTAAC CCCATCAGAC GCCACCCACA GAGAGTGGAC CCCAATGGCT 
     3001 ACATGATGAT GTCACCCAGT GGTAGTTGCT CCCCTGACAT TGGAGGTGGG TCCAGCAGCA 
     3061 GTAGCAGCAT CAGCGCAGCC CCTTCTGGGA GCAGCTATGG GAAGCCATGG ACAAATGGAG 
     3121 TAGGGGGGCA CCATACTCAT GCCCTTCCTC ATGCCAAACC TCCTGTTGAG AGTGGTGGCG 
     3181 GTAAGCTCTT GCCTTGCACA GGTGACTACA TGAACATGTC CCCAGTGGGA GATTCCAACA 
     3241 CCAGCAGCCC CTCAGAATGC TACTATGGCC CAGAAGATCC CCAGCACAAG CCGGTCCTCT 
     3301 CTTACTACTC ATTGCCAAGG TCCTTTAAGC ACACCCAGCG CCCTGGAGAG CCAGAGGAGG 
     3361 GTGCCAGGCA CCAGCATCTT CGTCTCTCTT CTAGCTCTGG ACGCCTTCGC TATACCGCAA 
     3421 CTGCCGAAGA TTCCTCCTCT TCTACCAGCA GCGACAGTCT GGGTGGGGGT TACTGTGGGG 
     3481 CTAGGCCAGA GTCAAGCCTC ACACATCCCC ACCACCATGT CTTGCAGCCC CACCTGCCTC 
     3541 GAAAGGTAGA CACAGCTGCA CAGACCAACA GCCGCCTGGC TCGACCCACA AGGCTGTCCT 
     3601 TGGGGGATCC CAAGGCAAGC ACCTTACCCC GGGTTCGAGA GCAGCAACAG CAGCAGCAGT 
     3661 CTTCCCTGCA CCCTCCCGAG CCCAAAAGCC CAGGAGAATA TGTGAATATT GAATTCGGGA 
     3721 GCGGCCAGCC TGGCTATTTA GCTGGCCCTG CAACTTCCCG TAGCTCTCCT TCAGTTCGAT 
     3781 GTCCACCCCA GCTCCACCCA GCTCCTAGAG AAGAGACTGG CTCGGAAGAG TACATGAACA 
     3841 TGGACTTGGG GCCAGGCCGG AGGGCAACCT GGCAGGAGAG TGGTGGAGTT GAGTTGGGCA 
     3901 GAATAGGCCC TGCACCTCCG GGGTCTGCTA CGGTTTGCAG GCCAACCCGT TCGGTGCCAA 
     3961 ATAGCCGTGG TGACTACATG ACCATGCAGA TAGGTTGTCC TCGTCAAAGC TATGTGGATA 
     4021 CCTCACCAGT GGCCCCAGTC AGCTATGCTG ACATGCGGAC AGGCATTGCT GCAGAGAAGG 
     4081 CGAGCCTGCC TAGACCCACA GGAGCTGCTC CTCCTCCATC CTCCACAGCC TCTTCTTCTG 
     4141 TTACACCTCA AGGAGCCACC GCTGAGCAAG CTACTCACTC TTCCTTGCTG GGAGGCCCTC 
     4201 AGGGACCTGG GGGCATGAGT GCATTCACCA GGGTGAACCT CAGTCCCAAC CATAACCAGA 
     4261 GTGCCAAAGT GATTCGCGCA GACACTCAAG GGTGCCGGAG GAGGCATAGC TCTGAGACCT 
     4321 TCTCAGCACC TACTCGGGCT GGCAATACGG TGCCCTTTGG AGCAGGGGCT GCAGTAGGGG 
     4381 GCAGCGGCGG TGGTGGTGGT GGCGGCAGTG AGGATGTAAA ACGCCACAGC TCTGCATCCT 
     4441 TTGAGAATGT GTGGCTGAGA CCTGGGGATC TAGGGGGAGT CTCCAAGGAG TCGGCTCCAG 
     4501 TGTGTGGGGC TGCTGGGGGT TTGGAGAAGA GTCTTAACTA CATAGACCTG GATTTGGCCA 
     4561 AGGAGCGCTC TCAGGACTGC CCCTCTCAAC AGCAGTCCCT ACCACCCCCT CCCCCTCACC 
     4621 AGCCCTTAGG CAGCAATGAG GGCAACTCCC CAAGACGCTC CAGTGAGGAT TTAAGCAACT 
     4681 ATGCCAGCAT CAGCTTCCAG AAGCAGCCAG AGGATCGTCA AACGCGTACG CGGCCGCTCG 
     4741 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4801 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4861 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4921 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4981 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5041 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5101 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5161 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5221 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5281 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5341 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5401 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5461 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5521 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5581 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5641 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5701 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5761 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5821 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5881 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5941 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6001 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6061 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6121 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6181 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6241 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6301 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6361 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6421 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6481 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6541 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6601 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6661 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6721 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6781 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6841 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6901 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6961 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7021 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7081 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7141 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7201 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7261 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7321 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7381 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7441 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7501 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7561 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7621 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7681 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7741 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7801 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7861 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7921 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7981 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8041 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8101 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8161 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8221 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8281 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8341 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8401 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8461 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8521 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//