LOCUS       MR226141                7545 bp    DNA     circular  SYN 
DEFINITION  Pml (Myc-DDK-tagged) - Mouse promyelocytic leukemia (Pml), transcript variant 2
ACCESSION   NM_178087
VERSION     NM_178087.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3700..3791 
                     /label="Myc-DDK"  
     misc_feature    3821..4406 
                     /label="PolyA signal"  
     misc_feature    3878..3897 
                     /label="Restriction Enzyme"  
     misc_feature    4682..5354 
                     /label="ColE1"  
     misc_feature    5784..6578 
                     /label="Kan/Neo"  
     misc_feature    6613..6915 
                     /label="SV40 ori"  
     misc_feature    7001..7478 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3675..3682 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3675 
                     /label="Pml"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAACTGAA CCAGTTTCCG TGCAGAAGGT ACCTGCACCC CCTGGATCTC 
     1081 CCTGTCGACA ACAGGACTCT GCCCTGACCC CCACGCCCAC CATGCCTCCC CCAGAGGAAC 
     1141 CCTCCGAAGA CTATGAACAC AGCCAAAGCC CTGCAGAGCA AGCCATACAG GAGGAATTTC 
     1201 AGTTTCTGCG CTGCCCGAGC TGCCAGGCCC AAGCCAAGTG CCCCAAACTG CTGCCTTGCC 
     1261 TGCACACGCT GTGCTCCGGA TGCCTGGAGG CGCCTGGCCT GCAGTGCCCC ATCTGCAAGG 
     1321 CTCCTGGGCA GGCCGATGCT AATGGGGAGG CCCTGGATAA CGTGTTCTTC GAGAGCCTGC 
     1381 AGCGGCGCCT GGCGGTGTTC CGGCAGATCG TGGATGCTCA GGCTGCGTGC ACCCGCTGCA 
     1441 AAGGCTTGGC CGACTTCTGG TGTTTCGAGT GTGAACAGCT CATTTGCAGC AAGTGCTTCG 
     1501 AAGCACACCA GTGGTACCTC AAGCATGAGG CCCGGCCCCT GGCTGATCTC CGCGACAATT 
     1561 CAGTGAGCAG CTTCCTCGAC AGTACGCGCA AGTCCAATAT CTTCTGCTCC AATACCAACC 
     1621 ACCGCAACCC TGCGCTGACT GACATCTACT GCCGAGGCTG CGCCAAGCCT CTGTGTTGCA 
     1681 CATGCGCGCT CCTGGACCGC AACCACAGCC ATCTCCATTG CGATATTGGT GAGGAGATTC 
     1741 AGCAGTGGCA TGAGGAACTA GGCACCATGA CACAGACTCT GGAGGAGCAG GGCAGAACCT 
     1801 TCGACAGTGC CCATGCACAG ATGTGCTCAG CTATAGGACA GCTGGACCAC GCACGCGCAG 
     1861 ACATTGAGAA GCAGATCCGC GCACGCGTGC GCCAGGTGGT AGACTACGTG CAGGCTCAGG 
     1921 AGCGCGAGCT GCTCGAGGCG GTGAATGACC GCTACCAGCG CGACTACCAG GAAATAGCTG 
     1981 GCCAGCTGAG CTGCCTGGAA GCTGTGCTGC AGCGCATCCG CACTAGTGGG GCGCTGGTCA 
     2041 AGAGGATGAA GCTCTATGCC TCCGACCAGG AGGTGCTGGA TATGCACAGC TTTCTGCGCA 
     2101 AGGCACTCTG TAGCTTGCGC CAGGAGGAGC CCCAGAACCA GAAAGTCCAG CTGCTCACCA 
     2161 GAGGTTTCGA GGAGTTCAAG CTGTGCCTGC AGGACTTCAT CTCCTGCATC ACCCAGAGGA 
     2221 TAAATGCAGC TGTAGCCAGC CCAGAGGCAG CCAGCAATCA ACCAGAGGCA GCCAGCACTC 
     2281 ACCCAGTGAC AACCAGCACG CCTGAGGACC TTGAGCAGCC CAAAGAAGTG CAGAGTGTAC 
     2341 AGGCTCAGGC CCTAGAGCTG TCTAAGACCC AACCTGTGGC TATGGTAAAA ACAGTGCCTG 
     2401 GAGCACACCC TGTACCAGTG TATGCCTTTT CAATGCAAGG CCCCACCTAC AGAGAGGAGG 
     2461 CTTCTCAGAC AGTCGGCTCC ATGAAGAGGA AGTGCTCCCA CGAAGATTGC TCCAGGAAGA 
     2521 TCATCAAGAT GGAGTCCACA GAGGAGAACG AGGACAGGTT GGCCACAAGC TCCCCCGAGC 
     2581 AGTCCTGGCC CAGCACTTTC AAGGCCACCT CCCCTCCCCA TCTGGATGGG ACTTCCAACC 
     2641 CTGAGAGCAC CGTCCCTGAA AAAAAGATCC TCCTGCCCAA CAACAATCAT GTTACCAGTG 
     2701 ACACAGGGGA AACAGAGGAG CGAGTTGTGG TGATCAGCAG CTCAGAGGAT TCGGACACCG 
     2761 AGAATCTGTC CTCCCACGAG TTGGACGATA GCAGCAGTGA GTCCAGCAGC CTGCAGCTGG 
     2821 AGGGCCCCAA TTCCCTCAAG GCCTTGGATG AGAGCCTCGC CGAACCCCAC TTAGAAGACA 
     2881 GGACCCTGGT TTTCTTTGAC CTCAAGATTG ACAATGAAAC CCAGAAAATT AGCCAGCTGG 
     2941 CCGCGGTGAA CCGGGAAAGC AAGTTCCGTG TGCTCATCCA GCCCGAGGCC TTCAGTGTCT 
     3001 ACTCCAAAGC TGTCTCCCTG GAGGCGGGGC TCCGGCACTT CCTCAGCTTC CTCACCACCA 
     3061 TGCACCGTCC CATCTTGGCG TGTTCTAGGC TGTGGGGGCC GGGACTCCCC ATCTTCTTCC 
     3121 AGACCCTGAG TGATATTAAC AAGCTGTGGG AATTCCAGGA CACCATCTCA GGTTTCTTGG 
     3181 CCGTGCTGCC CCTCATCCGG GAACGCATAC CCGGCGCTAG CAGCTTCAAA CTTGGGAACC 
     3241 TAGCCAAGAC CTACCTGGCG AGAAACATGA GCGAACGCAG CGCTCTGGCT TCTGTGCTGG 
     3301 CCATGAGGGA CTTGTGCTGC CTCCTTGAGA TCTCCCCAGG ACTGCCGCTG GCCCAGCATA 
     3361 TCTACTCCTT TAGTAGCTTG CAGTGTTTTG CTTCCCTGCA GCCCCTAATT CAGGCCAGCG 
     3421 TCCTGCCACA GTCCGAGGCC CGCCTCTTGG CCCTCCACAA TGTGAGCTTT GTAGAGTTAC 
     3481 TGAATGCATA TCGCACCAAC AGGCAAGAAG GCTTGAAGAA GTATGTCCAC TATCTGAGCC 
     3541 TGCAGACCAC CCCGTTGTCA TCGTCGGCTT CCACCCAAGT TGCCCAATTC CTGCAGGCTC 
     3601 TTAGCACCCA CATGGAAGGA CTGTTGGAAG GCCATGCCCC TGCTGGGGCA GAAGGCAAAG 
     3661 CTGAAAGTAA GGGATGCCTG GCCAGCGGAC CGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3721 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3781 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3841 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3901 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3961 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4021 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4081 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4141 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4201 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4261 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4321 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4381 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4441 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4501 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4561 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4621 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4681 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4741 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4801 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4861 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4921 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4981 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5041 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5101 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5161 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5221 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5281 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5341 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5401 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5461 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5521 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5581 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5641 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5701 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5761 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5821 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5881 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     5941 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6001 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6061 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6121 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6181 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6241 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6301 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6361 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6421 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6481 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6541 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6601 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6661 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6721 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6781 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6841 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6901 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6961 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7021 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7081 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7141 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7201 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7261 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7321 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7381 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7441 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7501 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//