LOCUS       MR226124                8367 bp    DNA     circular  SYN 
DEFINITION  Rtn4 (Myc-DDK-tagged) - Mouse reticulon 4 (Rtn4), transcript variant 1
ACCESSION   NM_194054
VERSION     NM_194054.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4524..4615 
                     /label="Myc-DDK"  
     misc_feature    4645..5230 
                     /label="PolyA signal"  
     misc_feature    4702..4721 
                     /label="Restriction Enzyme"  
     misc_feature    5506..6178 
                     /label="ColE1"  
     misc_feature    6608..7402 
                     /label="Kan/Neo"  
     misc_feature    7437..7739 
                     /label="SV40 ori"  
     misc_feature    7825..8302 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4506..4512 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4506 
                     /label="Rtn4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAGACATA GACCAGTCGT CGCTGGTCTC CTCGTCCGCG GATAGCCCGC 
     1081 CCCGGCCCCC GCCCGCTTTC AAGTACCAGT TCGTGACGGA GCCCGAGGAC GAGGAGGACG 
     1141 AGGAAGACGA GGAGGAGGAG GAGGACGACG AGGACCTGGA GGAATTGGAG GTGCTGGAGA 
     1201 GGAAGCCCGC AGCCGGGCTG TCCGCGGCTC CGGTGCCCCC CGCCGCCGCA CCGCTGCTGG 
     1261 ACTTCAGCAG CGACTCGGTG CCCCCCGCGC CCCGCGGGCC GCTGCCGGCC GCGCCCCCCA 
     1321 CCGCCCCTGA GAGGCAGCCG TCCTGGGAAC GCAGCCCCGC GGCGTCCGCG CCATCCCTGC 
     1381 CGCCCGCTGC CGCAGTCCTG CCCTCCAAGC TCCCGGAGGA CGACGAGCCT CCAGCGCGGC 
     1441 CTCCGGCGCC AGCCGGCGCG AGCCCCCTAG CGGAGCCCGC CGCGCCCCCT TCCACGCCGG 
     1501 CCGCGCCCAA GCGCAGGGGC TCGGGCTCAG TGGATGAGAC CCTTTTTGCT CTTCCTGCTG 
     1561 CATCTGAGCC TGTGATACCC TCCTCTGCAG AAAAAATTAT GGATTTGAAG GAGCAGCCAG 
     1621 GTAACACTGT TTCGTCTGGT CAAGAGGATT TCCCATCTGT CCTGTTTGAA ACTGCTGCCT 
     1681 CTCTTCCTTC TCTATCTCCT CTCTCAACTG TTTCTTTTAA AGAACACGGA TACCTTGGTA 
     1741 ACTTATCAGC AGTGGCATCC ACAGAAGGAA CTATTGAAGA AACTTTAAAT GAAGCTTCTA 
     1801 GAGAATTGCC AGAGAGGGCA ACAAATCCAT TTGTAAATAG AGAGTCAGCA GAGTTTTCAG 
     1861 TATTAGAATA CTCAGAAATG GGATCATCTT TCAATGGCTC CCCAAAAGGA GAGTCAGCCA 
     1921 TGTTAGTAGA AAACACTAAG GAAGAAGTAA TTGTGAGGAG TAAAGACAAA GAGGATTTAG 
     1981 TTTGTAGTGC AGCCCTTCAT AATCCACAAG AGTCACCTGC GACCCTTACT AAAGTGGTTA 
     2041 AAGAAGACGG AGTTATGTCT CCAGAAAAGA CAATGGACAT TTTTAATGAA ATGAAAATGT 
     2101 CAGTGGTAGC ACCTGTGAGG GAAGAGTATG CAGATTTTAA GCCATTTGAA CAAGCATGGG 
     2161 AAGTGAAAGA TACTTATGAG GGAAGTAGGG ATGTGCTGGC TGCTAGAGCT AATATGGAAA 
     2221 GTAAAGTGGA CAAAAAATGC TTTGAAGATA GCCTGGAGCA AAAAGGTCAT GGGAAGGATA 
     2281 GTGAAAGCAG AAATGAGAAT GCTTCTTTCC CCAGGACCCC AGAACTTGTG AAGGACGGCT 
     2341 CCAGAGCGTA CATCACCTGT GATTCCTTTA GCTCAGCAAC CGAGAGTACT GCAGCAAACA 
     2401 TTTTCCCTGT GCTAGAAGAT CACACTTCAG AAAACAAAAC AGATGAAAAA AAAATAGAAG 
     2461 AAAGGAAGGC CCAAATTATA ACAGAGAAGA CTAGCCCCAA AACGTCAAAT CCTTTCCTTG 
     2521 TAGCAATACA TGATTCTGAG GCAGATTATG TCACAACAGA TAATTTATCA AAGGTGACTG 
     2581 AGGCAGTAGT GGCAACCATG CCTGAAGGTC TAACGCCAGA TTTAGTTCAG GAAGCATGTG 
     2641 AAAGTGAACT GAACGAAGCC ACAGGTACAA AGATTGCTTA TGAAACAAAA GTGGACTTGG 
     2701 TCCAGACATC AGAAGCTATA CAAGAGTCAA TTTACCCCAC AGCACAGCTT TGCCCATCAT 
     2761 TTGAGGAAGC TGAAGCAACT CCGTCACCAG TTTTGCCTGA TATTGTTATG GAAGCGCCAT 
     2821 TAAATTCTCT CCTTCCAAGC ACTGGTGCTT CTGTAGCGCA GCCCAGTGCA TCCCCACTAG 
     2881 AAGTACCGTC TCCAGTTAGT TATGACGGTA TAAAGCTTGA GCCTGAAAAT CCCCCACCAT 
     2941 ATGAAGAAGC CATGAGTGTA GCACTAAAAA CATCGGACTC AAAGGAAGAA ATTAAAGAGC 
     3001 CTGAAAGTTT TAATGCAGCT GCTCAGGAAG CAGAAGCTCC TTATATATCC ATTGCATGTG 
     3061 ATTTAATTAA AGAAACAAAG CTCTCCACTG AGCCAAGTCC AGAGTTCTCT AATTATTCAG 
     3121 AAATAGCAAA ATTTGAGAAG TCGGTGCCTG ATCACTGTGA GCTCGTGGAT GATTCCTCAC 
     3181 CCGAATCTGA ACCAGTTGAC TTATTTAGTG ATGATTCAAT TCCTGAAGTC CCACAAACAC 
     3241 AAGAGGAGGC TGTGATGCTA ATGAAGGAGA GTCTCACTGA AGTGTCTGAG ACAGTAACAC 
     3301 AACACAAACA TAAGGAGAGA CTTAGTGCTT CACCTCAGGA GGTAGGAAAG CCATATTTAG 
     3361 AGTCTTTTCA GCCCAATTTA CATATTACAA AAGATGCTGC ATCTAATGAA ATTCCAACAT 
     3421 TGACCAAAAA GGAGACAATT TCTTTGCAAA TGGAAGAGTT TAATACTGCA ATTTATTCCA 
     3481 ATGATGACTT ACTTTCTTCT AAGGAAGACA AAATGAAAGA AAGTGAAACA TTTTCCGATT 
     3541 CATCTCCCAT TGAGATAATA GATGAGTTTC CCACATTTGT CAGTGCTAAA GATGATTCTC 
     3601 CTAAGGAGTA CACTGACCTA GAAGTATCCA ACAAAAGTGA AATTGCTAAT GTCCAGAGCG 
     3661 GGGCCAATTC GTTGCCTTGC TCAGAATTGC CCTGTGACCT TTCTTTCAAG AATACATATC 
     3721 CTAAAGATGA AGCACATGTC TCAGATGAAT TCTCCAAAAG TAGGTCCAGT GTATCTAAGG 
     3781 TGCCCTTATT GCTTCCAAAT GTTTCTGCTT TGGAATCTCA AATAGAAATG GGCAACATAG 
     3841 TTAAACCCAA AGTACTTACG AAAGAAGCAG AGGAAAAACT TCCTTCTGAT ACAGAGAAAG 
     3901 AGGACAGATC CCTGACAGCT GTATTGTCAG CAGAGCTGAA TAAAACTTCA GTTGTTGACC 
     3961 TCCTGTACTG GAGAGACATT AAGAAGACTG GAGTGGTGTT TGGTGCCAGC TTATTCCTGC 
     4021 TGCTGTCTCT GACAGTGTTC AGCATTGTCA GTGTAACGGC CTACATTGCC TTGGCCCTGC 
     4081 TCTCTGTGAC TATCAGCTTT AGGATATATA AGGGTGTGAT CCAAGCTATC CAGAAATCAG 
     4141 ATGAAGGCCA CCCATTCAGG GCATATTTGG AATCTGAAGT TGCCATATCA GAGGAATTGG 
     4201 TTCAGAAATA TAGTAATTCT GCTCTTGGTC ATGTGAACAG CACAATAAAA GAATTGAGGC 
     4261 GTCTCTTCTT AGTTGATGAT TTAGTTGATT CCCTGAAGTT TGCAGTGTTG ATGTGGGTAT 
     4321 TTACTTACGT TGGTGCCTTG TTCAATGGTT TGACACTACT GATTTTAGCT CTGATCTCAC 
     4381 TCTTCAGTAT TCCTGTTATA TATGAACGGC ATCAGGCGCA GATAGATCAT TATCTAGGAC 
     4441 TTGCAAACAA GAGCGTTAAG GATGCCATGG CCAAAATCCA AGCAAAAATC CCTGGATTGA 
     4501 AGCGCAAAGC AGAAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4561 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4621 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4681 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4741 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4801 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4861 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4921 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4981 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5041 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5101 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5161 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5221 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5281 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5341 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5401 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5461 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5521 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5581 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5641 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5701 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5761 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5821 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5881 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5941 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6001 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6061 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6121 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6181 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6241 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6301 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6361 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6421 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6481 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6541 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6601 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6661 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6721 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6781 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6841 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6901 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6961 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7021 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7081 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7141 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7201 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7261 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7321 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7381 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7441 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7501 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7561 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7621 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7681 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7741 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7801 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7861 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7921 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7981 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8041 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8101 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8161 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8221 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8281 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8341 ATTTAACAAA AATTTAACGC GAATTTT
//