LOCUS       MR226114                8268 bp    DNA     circular  SYN 
DEFINITION  Lats1 (Myc-DDK-tagged) - Mouse large tumor suppressor (Lats1)
ACCESSION   NM_010690
VERSION     NM_010690.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4425..4516 
                     /label="Myc-DDK"  
     misc_feature    4546..5131 
                     /label="PolyA signal"  
     misc_feature    4603..4622 
                     /label="Restriction Enzyme"  
     misc_feature    5407..6079 
                     /label="ColE1"  
     misc_feature    6509..7303 
                     /label="Kan/Neo"  
     misc_feature    7338..7640 
                     /label="SV40 ori"  
     misc_feature    7726..8203 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4407..4413 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4407 
                     /label="Lats1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGAGGGGT GAAAAGCCAG AAGGGTACAG ACAGATGAGG CCTAAGACCT 
     1081 TTCCTGCCAG CAACTACCCT GGCAGCAGCC GACAGATGCT GCAGGAGATC CGAGAATCCC 
     1141 TGAGGAATTT ATCAAAACCA TCTGATGCTT CGAAGGCCGA GCATAACCTG AATAAGATGT 
     1201 CGACTGAAGA TCCCAGGCAG GTGAGAAATC CACCCAAATT TGGCACACAT CATAAAGCCT 
     1261 TGCAAGAAAT TCGAAACTCT CTACTACCAT TTGCAAATGA AACAAGTTCT TCCCGGAGCC 
     1321 CTTCAGAAGT TAATCCACAG ATGTTTCAGG ATTTGCAGGC TGCTGGCTTT GATGAGGACA 
     1381 TGGTTATTCA AGCTCTTCAG AAAACCAATA ACAGAAGCAT AGAAGCCGCT GTTGAATTCA 
     1441 TTAGTAAAAT GAGTTACCAA GACCCTCGTC GAGAGCAGAT GTCTGCAGCA GCTGCCAGGC 
     1501 CTATTAATGC CACCATGAAA CCAGGAAATG TGCAACATTC AATTAACCGA AAACAAAGCT 
     1561 GGAAAGGTTC TAAAGAGTCT CTAGTTCCTC AGAGACACGG CCCATCTCTA GGAGAAAATG 
     1621 TGGTTTATCG TTCTGAAAGC CCCAACTCAC AGGCGGATGT AGGAAGACCT CTGTCTGGAT 
     1681 CCGGCATTGC AGCATTTGCT CAAGCTCACC CAAGCAATGG ACAGAGAGTG AACCCCCCAC 
     1741 CACCACCTCA AGTTAGGAGT GTTACTCCTC CACCACCTCC GAGAGGCCAG ACCCCACCTC 
     1801 CCCGAGGCAC CACTCCCCCT CCCCCCTCAT GGGAACCAAG CTCTCAGACA AAGCGCTACT 
     1861 CTGGGAACAT GGAGTACGTA ATCTCCCGAA TCTCCCCTGT TCCACCTGGG GCGTGGCAGG 
     1921 AGGGCTACCC TCCACCACCT CTTACCACTT CTCCCATGAA TCCCCCTAGC CAGGCTCAGA 
     1981 GGGCCATTAG TTCTGTTCCA GTTGGTAGAC AACCCATCAT CATGCAGAGT ACTAGCAAAT 
     2041 TTAACTTTAC ACCAGGGCGA CCTGGAGTTC AGAATGGTGG TGGTCAGTCT GATTTTATCG 
     2101 TGCACCAAAA TGTCCCCACT GGTTCTGTGA CTCGGCAGCC ACCACCTCCA TATCCTCTGA 
     2161 CCCCAGCTAA TGGACAAAGC CCCTCTGCTT TACAAACAGG GGCTTCTGCT GCTCCACCAT 
     2221 CATTCGCCAA TGGAAACGTT CCTCAGTCGA TGATGGTGCC CAACAGGAAC AGTCATAACA 
     2281 TGGAGCTTTA TAATATTAAT GTCCCTGGAC TGCAAACAGC CTGGCCCCAG TCGTCTTCTG 
     2341 CTCCTGCGCA GTCATCCCCA AGCGGTGGGC ATGAAATTCC TACATGGCAA CCTAACATAC 
     2401 CAGTGAGGTC AAATTCTTTT AATAACCCAT TAGGAAGTAG AGCAAGTCAC TCTGCTAATT 
     2461 CTCAGCCTTC TGCCACTACA GTCACTGCCA TCACACCCGC TCCTATTCAA CAGCCCGTGA 
     2521 AAAGCATGCG CGTCCTGAAA CCAGAGCTGC AGACTGCTTT AGCCCCAACC CATCCTTCTT 
     2581 GGATGCCACA GCCAGTTCAG ACTGTTCAGC CTACCCCTTT TTCTGAGGGT ACAGCTTCAA 
     2641 GTGTGCCTGT CATCCCACCT GTTGCTGAAG CTCCAAGCTA TCAAGGTCCA CCACCGCCTT 
     2701 ATCCAAAACA TCTGCTACAC CAAAACCCAT CTGTCCCTCC ATATGAGTCA GTAAGTAAGC 
     2761 CCTGCAAAGA TGAACAGCCT AGCTTACCCA AGGAAGATGA TAGTGAGAAG AGTGCGGACA 
     2821 GTGGTGACTC TGGGGATAAA GAAAAGAAAC AGATTACAAC TTCACCTATC ACTGTTCGGA 
     2881 AAAACAAGAA AGATGAAGAA CGAAGAGAGT CTCGGATTCA GAGTTACTCC CCACAGGCCT 
     2941 TTAAGTTCTT CATGGAGCAG CACGTAGAGA ACGTCCTGAA GTCTCATCAG CAGCGTCTGC 
     3001 ATCGGAAGAA GCAGCTAGAA AATGAAATGA TGCGGGTTGG ATTATCTCAA GATGCCCAGG 
     3061 ATCAAATGAG AAAGATGCTT TGCCAGAAAG AGTCTAACTA TATTCGTCTT AAAAGGGCTA 
     3121 AAATGGACAA GTCTATGTTT GTAAAGATAA AGACATTAGG AATAGGAGCG TTTGGTGAAG 
     3181 TCTGTCTAGC AAGAAAAGTC GATACTAAAG CTTTGTATGC AACAAAGACT CTTCGAAAGA 
     3241 AAGACGTTCT GCTCCGAAAT CAGGTGGCTC ATGTGAAAGC GGAGAGGGAT ATCCTAGCAG 
     3301 AAGCCGACAA TGAGTGGGTG GTCCGCCTGT ACTACTCTTT CCAGGACAAG GACAACTTGT 
     3361 ACTTTGTGAT GGACTACATT CCTGGGGGGG ATATGATGAG CCTATTAATT AGAATGGGCA 
     3421 TCTTTCCTGA AAATCTGGCA CGATTCTACA TAGCAGAACT TACCTGTGCA GTTGAAAGTG 
     3481 TTCATAAAAT GGGTTTTATT CATAGAGATA TTAAACCTGA TAACATTTTG ATTGACCGTG 
     3541 ATGGCCATAT TAAATTGACT GACTTTGGCT TGTGCACTGG CTTCAGATGG ACACATGACT 
     3601 CCAAGTACTA CCAGAGTGGG GATCACCCAC GGCAAGATAG CATGGATTTC AGTAACGAAT 
     3661 GGGGAGATCC TTCCAATTGT CGGTGTGGGG ACAGACTGAA GCCACTGGAG CGGAGAGCTG 
     3721 CTCGCCAGCA CCAGCGATGT CTAGCCCATT CTCTGGTTGG GACTCCCAAT TATATTGCAC 
     3781 CTGAAGTGCT ACTGCGAACA GGATATACAC AGCTGTGTGA CTGGTGGAGT GTTGGTGTTA 
     3841 TTCTTTTTGA AATGTTGGTG GGACAACCTC CTTTCTTGGC ACAAACCCCA TTAGAAACAC 
     3901 AAATGAAGGT TATCAACTGG CAAACTTCTC TTCACATCCC TCCTCAAGCT AAGCTGAGTC 
     3961 CTGAAGCCTC TGACCTCATT ATCAAACTTT GTCGAGGACC AGAAGACCGC CTCGGCAAGA 
     4021 ACGGTGCTGA TGAGATAAAG GCTCATCCAT TTTTTAAGAC CATCGATTTC TCTAGTGATC 
     4081 TGAGACAGCA GTCTGCTTCA TACATCCCTA AAATCACGCA TCCAACAGAT ACATCCAATT 
     4141 TCGACCCTGT TGATCCTGAT AAATTGTGGA GCGATGGCAG CGAGGAGGAA AATATCAGTG 
     4201 ACACTCTGAA CGGATGGTAT AAAAATGGGA AGCACCCCGA GCACGCTTTC TATGAGTTCA 
     4261 CCTTTCGGAG GTTTTTTGAT GACAATGGCT ACCCATATAA TTATCCAAAG CCTATTGAGT 
     4321 ATGAATACAT TCATTCACAG GGCTCAGAAC AACAGTCTGA TGAAGATGAT CAACACACAA 
     4381 GCTCCGATGG AAACAACCGA GATCTAGTGT ATGTTACGCG TACGCGGCCG CTCGAGCAGA 
     4441 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4501 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4561 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4621 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4681 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4741 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4801 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4861 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4921 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4981 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5041 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5101 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5161 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5221 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5281 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5341 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5401 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5461 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5521 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5581 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5641 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5701 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5761 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5821 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5881 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5941 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6001 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6061 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6121 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6181 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6241 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6301 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6361 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6421 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6481 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6541 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6601 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6661 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6721 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6781 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6841 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6901 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6961 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7021 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7081 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7141 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7201 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7261 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7321 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7381 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7441 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7501 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7561 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7621 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7681 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7741 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7801 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7861 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7921 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7981 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8041 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8101 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8161 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8221 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//