LOCUS       MR226113                8019 bp    DNA     circular  SYN 
DEFINITION  Rtn4 (Myc-DDK-tagged) - Mouse reticulon 4 (Rtn4), transcript variant 5
ACCESSION   NM_194051
VERSION     NM_194051.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4176..4267 
                     /label="Myc-DDK"  
     misc_feature    4297..4882 
                     /label="PolyA signal"  
     misc_feature    4354..4373 
                     /label="Restriction Enzyme"  
     misc_feature    5158..5830 
                     /label="ColE1"  
     misc_feature    6260..7054 
                     /label="Kan/Neo"  
     misc_feature    7089..7391 
                     /label="SV40 ori"  
     misc_feature    7477..7954 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4158..4164 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4158 
                     /label="Rtn4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCCCCCG CTGGCGGGAG GCGGGCAGAA GGGTGGGGCG GCCTCGGAAG 
     1081 CCTGGGTGCC TTCATTGTTT GTCGGGGTTT CGGGGAGCAC GTGCACCGCT GCCAAGTCGC 
     1141 TGGTTCCCAT TCCAGCACGA AGTTCTCGCC TATCAGCAGC TAGGAATGAG ACCCTTTTTG 
     1201 CTCTTCCTGC TGCATCTGAG CCTGTGATAC CCTCCTCTGC AGAAAAAATT ATGGATTTGA 
     1261 AGGAGCAGCC AGGTAACACT GTTTCGTCTG GTCAAGAGGA TTTCCCATCT GTCCTGTTTG 
     1321 AAACTGCTGC CTCTCTTCCT TCTCTATCTC CTCTCTCAAC TGTTTCTTTT AAAGAACACG 
     1381 GATACCTTGG TAACTTATCA GCAGTGGCAT CCACAGAAGG AACTATTGAA GAAACTTTAA 
     1441 ATGAAGCTTC TAGAGAATTG CCAGAGAGGG CAACAAATCC ATTTGTAAAT AGAGAGTCAG 
     1501 CAGAGTTTTC AGTATTAGAA TACTCAGAAA TGGGATCATC TTTCAATGGC TCCCCAAAAG 
     1561 GAGAGTCAGC CATGTTAGTA GAAAACACTA AGGAAGAAGT AATTGTGAGG AGTAAAGACA 
     1621 AAGAGGATTT AGTTTGTAGT GCAGCCCTTC ATAATCCACA AGAGTCACCT GCGACCCTTA 
     1681 CTAAAGTGGT TAAAGAAGAC GGAGTTATGT CTCCAGAAAA GACAATGGAC ATTTTTAATG 
     1741 AAATGAAAAT GTCAGTGGTA GCACCTGTGA GGGAAGAGTA TGCAGATTTT AAGCCATTTG 
     1801 AACAAGCATG GGAAGTGAAA GATACTTATG AGGGAAGTAG GGATGTGCTG GCTGCTAGAG 
     1861 CTAATATGGA AAGTAAAGTG GACAAAAAAT GCTTTGAAGA TAGCCTGGAG CAAAAAGGTC 
     1921 ATGGGAAGGA TAGTGAAAGC AGAAATGAGA ATGCTTCTTT CCCCAGGACC CCAGAACTTG 
     1981 TGAAGGACGG CTCCAGAGCG TACATCACCT GTGATTCCTT TAGCTCAGCA ACCGAGAGTA 
     2041 CTGCAGCAAA CATTTTCCCT GTGCTAGAAG ATCACACTTC AGAAAACAAA ACAGATGAAA 
     2101 AAAAAATAGA AGAAAGGAAG GCCCAAATTA TAACAGAGAA GACTAGCCCC AAAACGTCAA 
     2161 ATCCTTTCCT TGTAGCAATA CATGATTCTG AGGCAGATTA TGTCACAACA GATAATTTAT 
     2221 CAAAGGTGAC TGAGGCAGTA GTGGCAACCA TGCCTGAAGG TCTAACGCCA GATTTAGTTC 
     2281 AGGAAGCATG TGAAAGTGAA CTGAACGAAG CCACAGGTAC AAAGATTGCT TATGAAACAA 
     2341 AAGTGGACTT GGTCCAGACA TCAGAAGCTA TACAAGAGTC AATTTACCCC ACAGCACAGC 
     2401 TTTGCCCATC ATTTGAGGAA GCTGAAGCAA CTCCGTCACC AGTTTTGCCT GATATTGTTA 
     2461 TGGAAGCGCC ATTAAATTCT CTCCTTCCAA GCACTGGTGC TTCTGTAGCG CAGCCCAGTG 
     2521 CATCCCCACT AGAAGTACCG TCTCCAGTTA GTTATGACGG TATAAAGCTT GAGCCTGAAA 
     2581 ATCCCCCACC ATATGAAGAA GCCATGAGTG TAGCACTAAA AACATCGGAC TCAAAGGAAG 
     2641 AAATTAAAGA GCCTGAAAGT TTTAATGCAG CTGCTCAGGA AGCAGAAGCT CCTTATATAT 
     2701 CCATTGCATG TGATTTAATT AAAGAAACAA AGCTCTCCAC TGAGCCAAGT CCAGAGTTCT 
     2761 CTAATTATTC AGAAATAGCA AAATTTGAGA AGTCGGTGCC TGATCACTGT GAGCTCGTGG 
     2821 ATGATTCCTC ACCCGAATCT GAACCAGTTG ACTTATTTAG TGATGATTCA ATTCCTGAAG 
     2881 TCCCACAAAC ACAAGAGGAG GCTGTGATGC TAATGAAGGA GAGTCTCACT GAAGTGTCTG 
     2941 AGACAGTAAC ACAACACAAA CATAAGGAGA GACTTAGTGC TTCACCTCAG GAGGTAGGAA 
     3001 AGCCATATTT AGAGTCTTTT CAGCCCAATT TACATATTAC AAAAGATGCT GCATCTAATG 
     3061 AAATTCCAAC ATTGACCAAA AAGGAGACAA TTTCTTTGCA AATGGAAGAG TTTAATACTG 
     3121 CAATTTATTC CAATGATGAC TTACTTTCTT CTAAGGAAGA CAAAATGAAA GAAAGTGAAA 
     3181 CATTTTCCGA TTCATCTCCC ATTGAGATAA TAGATGAGTT TCCCACATTT GTCAGTGCTA 
     3241 AAGATGATTC TCCTAAGGAG TACACTGACC TAGAAGTATC CAACAAAAGT GAAATTGCTA 
     3301 ATGTCCAGAG CGGGGCCAAT TCGTTGCCTT GCTCAGAATT GCCCTGTGAC CTTTCTTTCA 
     3361 AGAATACATA TCCTAAAGAT GAAGCACATG TCTCAGATGA ATTCTCCAAA AGTAGGTCCA 
     3421 GTGTATCTAA GGTGCCCTTA TTGCTTCCAA ATGTTTCTGC TTTGGAATCT CAAATAGAAA 
     3481 TGGGCAACAT AGTTAAACCC AAAGTACTTA CGAAAGAAGC AGAGGAAAAA CTTCCTTCTG 
     3541 ATACAGAGAA AGAGGACAGA TCCCTGACAG CTGTATTGTC AGCAGAGCTG AATAAAACTT 
     3601 CAGTTGTTGA CCTCCTGTAC TGGAGAGACA TTAAGAAGAC TGGAGTGGTG TTTGGTGCCA 
     3661 GCTTATTCCT GCTGCTGTCT CTGACAGTGT TCAGCATTGT CAGTGTAACG GCCTACATTG 
     3721 CCTTGGCCCT GCTCTCTGTG ACTATCAGCT TTAGGATATA TAAGGGTGTG ATCCAAGCTA 
     3781 TCCAGAAATC AGATGAAGGC CACCCATTCA GGGCATATTT GGAATCTGAA GTTGCCATAT 
     3841 CAGAGGAATT GGTTCAGAAA TATAGTAATT CTGCTCTTGG TCATGTGAAC AGCACAATAA 
     3901 AAGAATTGAG GCGTCTCTTC TTAGTTGATG ATTTAGTTGA TTCCCTGAAG TTTGCAGTGT 
     3961 TGATGTGGGT ATTTACTTAC GTTGGTGCCT TGTTCAATGG TTTGACACTA CTGATTTTAG 
     4021 CTCTGATCTC ACTCTTCAGT ATTCCTGTTA TATATGAACG GCATCAGGCG CAGATAGATC 
     4081 ATTATCTAGG ACTTGCAAAC AAGAGCGTTA AGGATGCCAT GGCCAAAATC CAAGCAAAAA 
     4141 TCCCTGGATT GAAGCGCAAA GCAGAAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4201 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4261 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4321 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4381 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4441 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4501 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4561 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4621 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4681 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4741 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4801 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4861 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4921 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4981 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5041 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5101 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5161 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5221 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5281 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5341 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5401 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5461 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5521 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5581 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5641 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5701 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5761 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5821 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5881 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5941 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6001 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6061 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6121 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6181 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6241 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6301 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6361 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6421 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6481 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6541 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6601 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6661 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6721 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6781 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6841 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6901 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6961 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7021 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7081 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7141 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7201 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7261 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7321 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7381 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7441 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7501 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7561 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7621 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7681 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7741 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7801 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7861 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7921 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7981 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//