LOCUS       MR226085                8583 bp    DNA     circular  SYN 
DEFINITION  Map4k4 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4)
ACCESSION   NM_008696
VERSION     NM_008696.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4740..4831 
                     /label="Myc-DDK"  
     misc_feature    4861..5446 
                     /label="PolyA signal"  
     misc_feature    4918..4937 
                     /label="Restriction Enzyme"  
     misc_feature    5722..6394 
                     /label="ColE1"  
     misc_feature    6824..7618 
                     /label="Kan/Neo"  
     misc_feature    7653..7955 
                     /label="SV40 ori"  
     misc_feature    8041..8518 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4722..4728 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4722 
                     /label="Map4k4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAACGAC TCTCCCGCGA AGAGCCTGGT GGACATTGAC CTGTCGTCCC 
     1081 TGCGGGACCC TGCTGGGATT TTTGAGCTGG TGGAAGTGGT TGGAAATGGC ACCTATGGAC 
     1141 AAGTCTATAA GGGTCGACAT GTTAAAACGG GTCAGCTGGC CGCCATCAAG GTTATGGACG 
     1201 TCACCGAGGA TGAAGAGGAA GAAATCAAAC TGGAGATAAA TATGCTGAAG AAGTATTCTC 
     1261 ATCATCGAAA TATTGCCACG TACTATGGTG CTTTCATTAA GAAGAGCCCT CCAGGACATG 
     1321 ATGACCAACT CTGGCTTGTT ATGGAGTTTT GTGGGGCTGG GTCCATCACA GACCTTGTGA 
     1381 AGAACACCAA AGGGAACACT CTCAAAGAAG ACTGGATTGC TTACATCTCC AGGGAAATCC 
     1441 TCAGGGGATT GGCACATCTC CATATTCACC ACGTTATTCA CCGAGATATC AAGGGCCAAA 
     1501 ATGTGCTGCT GACCGAGAAT GCTGAGGTGA AACTTGTTGA TTTTGGTGTA AGCGCTCAGC 
     1561 TGGACAGGAC GGTTGGACGG AGAAATACGT TCATAGGCAC ACCCTACTGG ATGGCTCCAG 
     1621 AGGTCATCGC CTGTGATGAG AACCCAGACG CCACTTACGA CTACAGAAGT GACCTCTGGT 
     1681 CCTGTGGCAT CACAGCCATC GAGATGGCTG AAGGGGCCCC CCCTCTCTGT GACATGCATC 
     1741 CAATGAGAGC GCTGTTTCTC ATCCCCAGAA ACCCTCCTCC CAGGCTGAAG TCAAAAAAAT 
     1801 GGTCAAAGAA ATTTTTCAGC TTTATAGAAG GCTGTCTGGT GAAGAATTAC ATGCAGCGGC 
     1861 CCTCTACAGA GCAACTTTTA AAACACCCTT TCATAAGGGA TCAGCCCAAT GAAAGGCAGG 
     1921 TTCGAATCCA GCTTAAGGAT CACATAGACC GGACCAGAAA GAAGAGAGGC GAGAAAGATG 
     1981 AGACGGAGTA CGAGTACAGC GGGAGCGAGG AGGAGGAGGA GGAAGTGCCT GAGCAGGAGG 
     2041 GAGAGCCAAG TTCCATCGTC AATGTGCCTG GAGAGTCAAC TCTGCGACGT GATTTCCTGA 
     2101 GACTGCAGCA GGAGAACAAG GAGCGGTCTG AGGCTCTGCG GAGACAGCAG CTTCTGCAGG 
     2161 AGCAGCAGCT CCGGGAGCAG GAGGAGTATA AGAGGCAGCT GCTGGCTGAG AGGCAGAAGC 
     2221 GGATTGAACA GCAGAAAGAA CAGAGGAGGC GGCTGGAAGA GCAACAAAGA AGAGAACGGG 
     2281 AAGCCAGGAG GCAGCAGGAG CGTGAGCAGC GGCGGCGTGA ACAAGAGGAG AAGAGGCGTC 
     2341 TCGAGGAACT GGAAAGGCGG CGTAAAGAAG AGGAAGAGAG GAGACGGGCA GAAGAGGAGA 
     2401 AGAGGAGAGT GGAGAGGGAA CAGGAGTACA TCAGGCGGCA GCTAGAGGAG GAGCAGCGGC 
     2461 ACCTGGAGAT CCTGCAGCAG CAGCTGCTCC AGGAGCAGGC CATGTTACTG CACGACCACA 
     2521 GGAGGCCGCA CGCACAGCAG CAGCCGCCGC CCCCGCAGCA GCAGGACAGG AGCAAACCGA 
     2581 GCTTTCATGC TCCAGAGCCC AAGCCTCACT ATGACCCTGC TGACAGAGCT CGGGAGGTAC 
     2641 AGTGGTCCCA CCTGGCATCT CTCAAGAACA ATGTCTCCCC TGTCTCGAGA TCCCATTCCT 
     2701 TCAGTGACCC TTCTCCCAAA TTCGCACACC ACCATCTCCG CTCTCAGGAC CCATGTCCAC 
     2761 CTTCCCGCAG TGAGGGGCTC AGTCAGAGCT CTGACTCTAA GTCGGAGGTG CCCGAGCCCA 
     2821 CCCAAAAGGC TTGGTCTAGA TCAGACAGTG ACGAGGTGCC TCCAAGGGTT CCCGTGAGAA 
     2881 CGACCTCTCG TTCCCCTGTC CTCTCCCGTC GGGATTCCCC ACTGCAGGGC GGTGGACAGC 
     2941 AGAATAGCCA AGCAGGACAG AGAAATTCCA CCAGCAGTAT TGAGCCCCGG CTTCTTTGGG 
     3001 AGAGAGTGGA AAAGCTGGTC CCCAGGCCAG GCAGTGGCAG CTCTTCAGGG TCCAGCAACT 
     3061 CAGGATCCCA GCCTGGCTCC CATCCTGGGT CTCAGAGCGG CTCCGGTGAA CGCTTCAGAG 
     3121 TGAGATCATC ATCCAAGTCT GAAGGTTCTC CATCGCCACG CCAGGAAAGT GCAGCCAAAA 
     3181 AGCCTGACGA TAAGAAAGAA GTATTCCGGC CTCTCAAGCC TGCGGGAGAA GTGGACTTGA 
     3241 CTGCGTTGGC CAAAGAGCTT CGAGCAGTGG AAGATGTTCG GCCACCTCAC AAAGTAACAG 
     3301 ACTACTCATC CTCCAGTGAG GAGTCTGGGA CCACAGATGA GGAGGAGGAG GACGTGGAAC 
     3361 AGGAGGGGGC TGATGATTCT ACCTCGGGAC CAGAAGACAC CAGAGCAGCG TCATCTCCGA 
     3421 ACCTGAGCAA CGGTGAAACA GAATCTGTGA AAACAATGAT CGTTCATGAT GATGTAGAAA 
     3481 GTGAGCCAGC CATGACCCCG TCCAAGGAGG GCACCCTCAT CGTCCGCCAG ACTCAGTCCG 
     3541 CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA 
     3601 GGTTACTACA GATCTCTCCA TCTAGTGGGA CGACAGTGAC TTCCGTGGTG GGATTTTCCT 
     3661 GTGATGGGCT GAGACCAGAA GCCATAAGGC AAGATCCTAC TCGGAAAGGC TCAGTGGTCA 
     3721 ATGTGAACCC CACGAACACT AGGCCACAGA GTGATACCCC CGAGATTCGT AAATACAAGA 
     3781 AGAGATTTAA CTCAGAGATC CTGTGTGCTG CCTTATGGGG AGTGAACTTG CTGGTTGGTA 
     3841 CAGAGAGCGG TCTGATGCTC TTGGACAGAA GTGGCCAAGG GAAAGTATAC CCTCTGATCA 
     3901 GCCGAAGACG GTTCCAGCAA ATGGATGTGC TCGAAGGCCT AAATGTCCTG GTGACAATAT 
     3961 CTGGCAAAAA GGATAAGTTA CGTGTCTACT ATTTATCCTG GCTAAGAAAT AAGATTCTTC 
     4021 ATAATGATCC AGAGGTTGAG AAGAAGCAGG GGTGGACCAC TGTGGGCGAC TTGGAAGGGT 
     4081 GCGTGCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTAATT GCTTTGAAGA 
     4141 GTTCTGTGGA AGTCTATGCA TGGGCACCGA AGCCATATCA CAAATTTATG GCCTTTAAGT 
     4201 CATTTGGAGA ACTGCTACAT AAGCCATTAC TGGTGGATCT CACTGTGGAG GAAGGCCAGA 
     4261 GGTTGAAAGT GATCTATGGA TCCTGTGCGG GATTCCATGC TGTTGATGTG GATTCAGGCT 
     4321 CAGTCTATGA CATTTATCTA CCAACACATA TTCAGTGTAG CATCAAACCC CATGCAATCA 
     4381 TTATCCTCCC CAACACGGAT GGGATGGAGC TGCTGGTGTG CTACGAAGAC GAGGGGGTTT 
     4441 ACGTTAACAC GTACGGAAGG ATCACCAAGG ACGTGGTTCT GCAGTGGGGA GAGATGCCGA 
     4501 CATCTGTAGC ATATATCCGG TCGAATCAGA CAATGGGCTG GGGAGAGAAG GCAATAGAGA 
     4561 TACGATCTGT GGAAACTGGT CACTTGGATG GTGTATTTAT GCACAAAAGG GCTCAGAGAC 
     4621 TAAAGTTCCT GTGTGAACGC AATGACAAGG TGTTCTTTGC CTCCGTCCGC TCTGGTGGCA 
     4681 GCAGCCAGGT CTATTTCATG ACCTTAGGCA GGACTTCTCT TCTGAGCTGG ACGCGTACGC 
     4741 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4801 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4861 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4921 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4981 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5041 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5101 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5161 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5221 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5281 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5341 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5401 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5461 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5521 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5581 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5641 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5701 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5761 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5821 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5881 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5941 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6001 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6061 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6121 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6181 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6241 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6301 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6361 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6421 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6481 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6541 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6601 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6661 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6721 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6781 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6841 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6901 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6961 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7021 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7081 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7141 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7201 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7261 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7321 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7381 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7441 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7501 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7561 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7621 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7681 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7741 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7801 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7861 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7921 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7981 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8041 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8101 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8161 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8221 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8281 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8341 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8401 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8461 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8521 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8581 TTT
//