LOCUS       MR226052                8667 bp    DNA     circular  SYN 
DEFINITION  Myo6 (Myc-DDK-tagged) - Mouse myosin VI (Myo6)
ACCESSION   NM_001039546
VERSION     NM_001039546.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4824..4915 
                     /label="Myc-DDK"  
     misc_feature    4945..5530 
                     /label="PolyA signal"  
     misc_feature    5002..5021 
                     /label="Restriction Enzyme"  
     misc_feature    5806..6478 
                     /label="ColE1"  
     misc_feature    6908..7702 
                     /label="Kan/Neo"  
     misc_feature    7737..8039 
                     /label="SV40 ori"  
     misc_feature    8125..8602 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4806..4812 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4806 
                     /label="Myo6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAGATGGA AAGCCCGTTT GGGCACCCCA CCCCACAGAT GGATTTCAGA 
     1081 TGGGCAATAT TGTGGATATT GGCCCTGACA GCTTAACAAT TGAACCTTTG AACCAAAAAG 
     1141 GCAAGACATT TTTGGCTCTC ATAAACCAAG TATTTCCTGC AGAAGAGGAC AGCAAAAAAG 
     1201 ATGTGGAAGA TAACTGTTCA CTGATGTACT TAAATGAAGC CACACTCCTC CACAATGTCA 
     1261 AAGTTCGGTA CAGTAAAGAC AGAATCTATA CATATGTGGC CAACATTCTG ATTGCAGTGA 
     1321 ATCCATACTT TGATATACCT AAGATATATT CTTCAGATAC AATAAAGTCA TATCAAGGAA 
     1381 AATCTCTTGG GACGATGCCA CCCCATGTCT TTGCCATTGC TGATAAAGCT TTCCGAGACA 
     1441 TGAAGGTGCT CAAGATGAGC CAGTCTATCA TCGTATCTGG AGAATCAGGA GCTGGCAAAA 
     1501 CAGAAAATAC AAAGTTTGTT CTAAGATACC TGACAGAATC TTATGGAACA GGTCAAGATA 
     1561 TTGATGATAG AATTGTTGAA GCGAATCCAC TTTTAGAAGC ATTTGGAAAT GCAAAGACTG 
     1621 TTCGCAACAA TAATAGCAGT CGATTTGGAA AATTTGTAGA AATTCATTTC AATGAAAAGA 
     1681 GTTCGGTTGT TGGAGGATTT GTTTCCCATT ACCTTCTAGA GAAGTCTAGG ATCTGTGTTC 
     1741 AAGGCAAAGA GGAGCGGAAT TACCATATTT TCTACAGGCT CTGTGCTGGG GCTTCAGAAG 
     1801 ACATCAGGGA GAAGCTTCAC TTGAGCTCCC CAGATAATTT CCGGTATTTA AACCGGGGCT 
     1861 GCACTAGATT CTTCGCTAAC AAAGAAACGG ACAAACAGAT TTTACAGAAC CGAAAAAGTC 
     1921 CTGAGTATGT TAAGGCAGGT TCCTTGAAGG ATCCTCTATT AGACGACCAT GGAGATTTTA 
     1981 TCAGGATGTG CACAGCCATG AAAAAAATTG GTTTGGATGA TGAAGAAAAA CTTGATCTGT 
     2041 TTCGAGTTGT AGCTGGTGTC CTGCACCTTG GAAATATTGA TTTTGAGGAA GCCGGAAGCA 
     2101 CTTCAGGTGG CTGCAATCTG AAGAACAAAT CTGCCCCATC CCTGGAATAC TGTGCTGAGC 
     2161 TGCTGGGCTT GGACCAAGAT GATCTTCGAG TCAGTTTAAC CACTCGAGTC ATGCTCACCA 
     2221 CAGCAGGGGG CACCAAAGGA ACCGTTATAA AGGTCCCCCT GAAAGTGGAG CAGGCAAACA 
     2281 ATGCTCGCGA TGCTCTGGCA AAGACTGTCT ATAGCCATCT CTTTGATCAC GTGGTGAACA 
     2341 GGGTAAATCA GTGCTTTCCT TTTGAAACAT CTTCTTATTT TATTGGAGTC CTGGATATTG 
     2401 CTGGTTTCGA GTACTTTGAG CACAACAGCT TCGAGCAATT TTGTATCAAC TATTGCAATG 
     2461 AGAAACTCCA ACAGTTTTTT AATGAAAGGA TCCTGAAGGA GGAACAAGAA CTGTATCAGA 
     2521 AAGAGGGCCT GGGTGTTAAT GAAGTCCATT ACGTGGACAA TCAGGACTGC ATAGATTTAA 
     2581 TCGAAGTGAA GTTAGTGGGG ATCCTGGATA TTCTGGATGA AGAAAATCGT CTTCCCCAGC 
     2641 CGAGTGACCA GCACTTCACG TCTGTGGTTC ACCAGAAGCA CAAAGACCAC TTCCGGCTCA 
     2701 CTATTCCCAG GAAATCTAAG CTGGCAGTCC ACAGGAACCT CAGAGACGAT GAAGGCTTTA 
     2761 TCATCAGGCA CTTTGCAGGG GCCGTGTGCT ACGAGACAAC CCAGTTTGTG GAGAAAAATA 
     2821 ATGATGCACT ACATATGTCT CTTGAATCCT TGATATGTGA GTCCAGGGAT AAGTTTATAA 
     2881 GGGCATTATT TGAGTCATCT ACAAATAACA ACAAAGATAC GAAACAGAAG GCTGGAAAAC 
     2941 TTAGCTTCAT CAGCGTGGGA AACAAGTTCA AGACACAATT AAATTTGCTT CTGGATAAAC 
     3001 TTCGAAGTAC CGGAGCCAGC TTCATCCGCT GCATCAAACC CAACTTAAAG ATGACGAGTC 
     3061 ACCACTTCGA AGGCGCCCAG ATTCTGTCTC AACTTCAGTG TTCAGGTATG GTGTCTGTGT 
     3121 TGGACCTAAT GCAAGGGGGT TTCCCTTCAA GAGCCTCGTT CCATGAACTC TATAACATGT 
     3181 ACAAGAAGTA CATGCCAGAA AAACTTGCAA GATTGGATCC GAGGTTATTT TGTAAGGCTC 
     3241 TTTTTAAAGC TTTGGGATTA AATGAAGTTG ACTACAAGTT TGGGCTGACA AAAGTATTTT 
     3301 TCAGACCTGG CAAGTTTGCA GAATTTGATC AGATTATGAA GTCTGACCCT GATCACTTAG 
     3361 CAGAGTTGGT GAAAAGAGTC AACCTGTGGC TAGTCTGTAG TCGCTGGAAG AAAGTTCAGT 
     3421 GGTGTTCACT GTCAGTCATC AAGCTGAAAA ACAAAATAAA ATATCGAGCT GAAGCATGCA 
     3481 TTAAAATGCA GAAAACCATC CGAATGTGGC TTTGCAAAAG GAGGCACAAA CCACGCATTG 
     3541 ACGGCCTGGT TAAGGTGGGC ACACTCAAGA AACGACTCGA TAAGTTCAAT GAAGTCGTAA 
     3601 GTGCGCTGAA AGATGGGAAG CCGGAGGTGA ACAGACAGAT CAAAAATCTT GAAATTTCTA 
     3661 TTGATGCTCT AATGGCCAAA ATTAAGTCCA CCATGATGAC GAGGGAGCAG ATACAGAAAG 
     3721 AGTATGATGC ACTTGTTAAA AGCTCAGAAG ATCTCCTCAG TGCACTGCAG AAAAAGAAAC 
     3781 AGCAAGAGGA GGAGGCCGAG AGGCTGAGGC GCATCCAGGA GGAGATGGAG AAAGAAAGGA 
     3841 AGAGGCGTGA AGAAGATGAG GAACGTCGGC GGAAGGAGGA AGAGGAGAGG CGGATGAAAC 
     3901 TTGAGATGGA AGCGAAGAGA AAACAAGAAG AGGAAGAAAG GAAGAAGCGG GAAGATGATG 
     3961 AAAAGCGAAT CCAGGCTGAG GTGGAGGCAC AGCTGGCAAG ACAGCGGGAG GAGGAGTCCC 
     4021 AGCAGCAGGC GGTCCTGGCC CAGGAGTGCA GGGACAGGGA GTTGGCCCTG CGCATCGCCC 
     4081 AGAACGAGTC GGAGCTCATC AGCGATGAGG CACAGGGTGA CATGGCACTC CGAAGAGGTC 
     4141 CTGCGGTGCA AGCTACCAAG GCTGCTGCTG GTACCAAGAA ACATGATCTC AGTAAATGGA 
     4201 AATACGCAGA ACTACGCGAT ACAATCAATA CTTCCTGTGA CATTGAGCTC CTGGCAGCTT 
     4261 GCAGAGAAGA GTTTCACAGG AGACTGAAAG TGTATCATGC TTGGAAATCC AAGAACAAGA 
     4321 AAAGAAACAC TGAGACCGAG CAGCGCGCTC CCAAGTCGGT TACTGATTAT GATTTTGCAC 
     4381 CATTTTTGAA CAATTCACCT CAGCAGAATC CCGCAGCTCA GCTCCCTGCC AGGCAGCAGG 
     4441 AGATTGACAT GAAACGACAG CAGCGTTTCT TCCGCATCCC CTTCATCCGC CCTGCGGACC 
     4501 AGTACAAAGA CCCTCAGAAC AAGAAAAAGG GCTGGTGGTA TGCCCATTTT GACGGACCCT 
     4561 GGATTGCTCG GCAGATGGAG CTGCACCCTG ACAAGCCACC CATCCTTCTT GTAGCTGGCA 
     4621 AGGATGACAT GGAGATGTGT GAGCTGAATC TCGAGGAGAC GGGTCTGACT CGCAAGCGTG 
     4681 GTGCTGAGAT CTTGCCCAGA CAGTTTGAAG AGATCTGGGA GCGTTGTGGA GGCATCCAGT 
     4741 ACCTTCAGAG CGCCATTGAG AGCAGGCAGG CCCGGCCCAC CTATGCTACG GCCATGCTGC 
     4801 AGAACCTGCT CAAGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4861 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4921 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4981 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5041 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5101 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5161 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5221 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5281 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5341 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5401 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5461 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5521 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5581 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5641 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5701 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5761 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5821 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5881 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5941 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6001 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6061 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6121 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6181 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6241 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6301 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6361 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6421 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6481 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6541 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6601 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6661 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6721 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6781 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6841 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6901 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6961 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7021 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7081 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7141 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7201 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7261 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7321 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7381 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7441 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7501 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7561 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7621 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7681 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7741 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7801 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7861 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7921 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7981 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8041 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8101 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8161 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8221 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8281 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8341 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8401 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8461 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8521 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8581 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8641 ATTTAACAAA AATTTAACGC GAATTTT
//