LOCUS       MR226037                8646 bp    DNA     circular  SYN 
DEFINITION  Magi1 (Myc-DDK-tagged) - Mouse membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant 2
ACCESSION   NM_001083320
VERSION     NM_001083320.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4803..4894 
                     /label="Myc-DDK"  
     misc_feature    4924..5509 
                     /label="PolyA signal"  
     misc_feature    4981..5000 
                     /label="Restriction Enzyme"  
     misc_feature    5785..6457 
                     /label="ColE1"  
     misc_feature    6887..7681 
                     /label="Kan/Neo"  
     misc_feature    7716..8018 
                     /label="SV40 ori"  
     misc_feature    8104..8581 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4785..4791 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4785 
                     /label="Magi1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAAAATGCT GGCATAGTCC ACCCGGAGAA TGAGGAGGAG GAGGATGTCC 
     1081 CTGAAATGAA CAGTAGCTTT ACAGCCGACT CTGGAGACCA GGACGAGCAC ACTCTCCAAG 
     1141 AAGCAACGCT CCCGCCTGTG AATAGTAGCA TCCTCGCTGC TCCCATCACG GACCCTTCTC 
     1201 AGAAGTTCCC TCAGTACCTA CCTCTTTCTG CAGAGGATAA TTTAGGTCCT CTACCTGAAA 
     1261 ACTGGGAGAT GGCCTATACT GAAAATGGAG AAGTCTATTT CATAGACCAC AACACGAAAA 
     1321 CAACATCATG GTTAGACCCT CGGTGCCTGA ACAAACAGCA GAAGCCTCTG GAAGAATGTG 
     1381 AAGATGATGA AGGGGTACAC ACCGAGGAGC TGGACAGTGA ACTAGAGTTG CCTGCTGGCT 
     1441 GGGAAAAGAT TGAAGACCCT GTCTACGGTG TCTACTATGT AGACCACATC AACAGGAAGA 
     1501 CGCAATATGA AAACCCAGTC CTAGAAGCCA AACGGAAGAA ACAGCTTGAA CAGCAGCAGC 
     1561 AACAGCAGCA GCCTCAGCCA CCGCAGCCAG AAGAGTGGAC AGAGGATCAT GCATCTGTTG 
     1621 TGCCTCCTGT TGCTCCTTCC CATCCCCCGA GCAATCCGGA GCCAGCCAGG GAAACTCCAC 
     1681 TTCAGGGCAA ACCTTTTTTT ACAAGAAACC CCTCTGAGCT GAAAGGCAAG TTCATTCACA 
     1741 CGAAGCTACG GAAAAGCAGC CGAGGCTTTG GCTTCACGGT GGTTGGAGGA GACGAGCCTG 
     1801 ATGAGTTCCT GCAGATCAAG AGCCTCGTCC TCGATGGTCC TGCCGCACTG GATGGCAAGA 
     1861 TGGAGACAGG GGATGTAATT GTGAGTGTGA ATGACACCTG TGTTTTGGGA CACACACATG 
     1921 CTCAAGTTGT GAAAATCTTC CAGTCCATTC CCATTGGTGC CAGTGTGGAC CTTGAACTCT 
     1981 GCAGAGGTTA TCCATTGCCT TTTGACCCGG ATGACCCTAA TACAAGTTTA GTGACCTCGG 
     2041 TGGCCATTTT GGACAAAGAA CCAATTATTG TAAATGGACA AGAGACCTAC GATTCACCAG 
     2101 CGAGCCACAG TAGTAAAACA GGCAAAGTCA GCAGCATGAA GGATGCCAGG CCAAGCAGCC 
     2161 CTGCTGATGT GGCTTCCAAC AGCTCTCATG GTTATCCCAA CGACACAGTC TCCTTGGCTT 
     2221 CCTCCATAGC CACCCAGCCA GAGCTAATAA CTGTTCACAT AGTCAAAGGG CCAATGGGAT 
     2281 TTGGCTTTAC GATCGCAGAC AGTCCCGGTG GGGGTGGCCA AAGAGTGAAA CAGATTGTTG 
     2341 ACAGTCCACG CTGCAGAGGC CTCAAAGAAG GGGATCTTAT CGTGGAGGTG AATAAGAAGA 
     2401 ACGTGCAGGC CCTGACGCAC AATCAAGTCG TGGATATGCT GATTGAATGT CCCAAGGGAA 
     2461 GTGAGGTCAC ACTGTTGGTG CAGCGAGGAG GGCTACCAGT TCCCAAGAAG AGCCCAAAGT 
     2521 CGCCACTGGA GAGGAAAGAC AGCCAGAATA GCTCCCAGCA CAGCGTCTCC AGCCACCGGA 
     2581 GCCTGCACAC TGCGTCCCCG AGCCACGGCA TACAGGTGCT CCCTGAGTAC CTACCTGCAG 
     2641 ACGCCCCTGC TCCAGATCAG ACCGACAGCT CTGGGCAGAA AAAGCCAGAT CCTTTTAAAA 
     2701 TCTGGGCCCA GTCCAGGAGC ATGTATGAAA ACCGACCTAT GTCACCTTCG CCTGCATCAG 
     2761 GATTGAGCAA GGGTGAAAGA GACAGAGAAA TCAATTCCAC GAATTTTGGA GAATGTCAGA 
     2821 TTCCAGATTA CCAGGAACAG GACATCTTCC TCTGGAGAAA AGAAACCGGA TTTGGATTTA 
     2881 GGATTCTGGG TGGAAATGAA CCAGGGGAAC CCATTTATAT CGGTCACATC GTACCGCTGG 
     2941 GTGCTGCTGA CACAGACGGC CGCCTGAGGT CTGGAGATGA ATTAATCTGT GTGGATGGGA 
     3001 CACCAGTAAT TGGGAAATCA CACCAGCTCG TGGTCCAGCT TATGCAACAA GCTGCCAAGC 
     3061 AAGGCCATGT CAATCTCACA GTGAGGCGGA AAGTGGTCTT TGCCGTCCCC AAAGCAGAGA 
     3121 ATGAGGTGCC CTCACCAGCC TCATCACACC ACAGTAGCAA CCAGCCCGCG TCCCTGACGG 
     3181 AGGAGAAACG CACACCGCAA GGCAGCCAGA ACTCTCTGAA CACTGTGAGC TCTGGCAGCG 
     3241 GCAGCACCAG TGGCATTGGC AGTGGTGGCG GCGGGGGCAG CGGTGTGGTG AGCGCTGTGC 
     3301 TGCAGCCCTA TGATGTGGAG ATTCGGCGTG GGGAGAACGA GGGCTTTGGG TTTGTCATCG 
     3361 TGTCCTCCGT GAGCAGACCC GAAGCGGGCA CAACCTTCGC AGGCAATGCA TGTGTGGCTA 
     3421 TGCCTCACAA AATAGGTCGG ATTATTGAGG GGAGCCCTGC TGACCGCTGT GGCAAGCTGA 
     3481 AAGTAGGAGA CCGGATCTTG GCAGTAAATG GATGTTCCAT CACCAACAAA TCCCATTCTG 
     3541 ACATTGTCAA CCTAATCAAA GAAGCGGGCA ACACAGTGAC TCTCCGCATC ATCCCCGGGG 
     3601 ATGAGTCCTC AAATGCCACG CTGCTGACTA ATGCTGAGAA GATTGCCACC ATCACCACCA 
     3661 CTCATGCCCC CTCTCAGCAG GGGACCCAGG AAACAAGGAC CACCACCAAA CCAAAGCAGG 
     3721 ATTCTCAGTT TGAGTTCAAA GGACCGCAGG CTGCACAGGA GCAAGATTTC TACACTGTGG 
     3781 AATTGGAAAG AGGGGCCAAG GGATTTGGCT TTAGTCTTCG AGGGGGCCGA GAATATAACA 
     3841 TGGATCTTTA TGTTCTGCGC TTGGCAGAGG ATGGTCCTGC AGAAAGATGT GGGAAGATGA 
     3901 GGATTGGCGA TGAAATTCTA GAGATCAATG GTGAGACCAC CAAAAACATG AAACACTCTC 
     3961 GGGCCATAGA ACTGATCAAG AATGGCGGCC GCAGGGTCCG TCTGTTTCTG CGGCGGGGAG 
     4021 ACGGCTCAGT CCCAGAATAT GACCCCAGCA GCGACAGGAA CGGCCCCTCC ACCGGTGCAC 
     4081 AAGGTGTTCC GGAAGTGAGG CCGGGGCCGC CAGACCACAG ACCGCATCCA GCCTTGGAGT 
     4141 CCAGTTACCC ACCCGAACTT CACAAATCAT CACAACATGC CGAAAAGCGA GCACACGCGA 
     4201 AGGATCCAAA AGGCAACAGG GAGCACAGCA AACAACCCAA CGAACATCAC ACCTGGAATG 
     4261 GAACTTCTAG AAAACAGGAC AGCGGGGCAT GCCGGCCCAA AGACCGGCCA CCCGACGCAT 
     4321 GGAGAGAGGC GCAGCCAGAG CGGACAGCCA CCAATGGTTC AAAGAGGAGG TCACCGGAGA 
     4381 AGCGCAGGGA AGGCACCCGC AGCGCTGACA ACACTTTGGA AAGAAGGGAG AAGCATGAGA 
     4441 AGAGAAGAGA GATATCTCCC GAGAGGAAGC GAGAGCGTTC ACCCACCCGG AGAAAAGATA 
     4501 GCTCCCCGAG CCGCCGGCGG AGGTCCCTCG AAAGACTCCT GGATCAAAGA CGGTCCCCAG 
     4561 AGCGCAGAAG AGGGGGCTCC CCCGAGAGGA GAGCCAAATC CACCGACAGG AGGAGGGCCA 
     4621 GGTCCCCTGA GCGCAGGCGA GAGCGGTCAC TGGACAAAAG GAACCGGGAC GACAAGGTTG 
     4681 GCCACCGAGA AAGAGAGGAG GCTGGTCTGA AGCTGGAAGC GGGGAGAAGC CCCCGAAATC 
     4741 CCCCAGAGCA GAGAAGGCGG CCTTACAAAG AATGTAGCAC CGACCTCAGC ATCACGCGTA 
     4801 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4861 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4921 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4981 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5041 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5101 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5161 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5221 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5281 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5341 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5401 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5461 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5521 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5581 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5641 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5701 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5761 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5821 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5881 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5941 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6001 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6061 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6121 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6181 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6241 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6301 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6361 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6421 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6481 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6541 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6601 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6661 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6721 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6781 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6841 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6901 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6961 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7021 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7081 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7141 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7201 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7261 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7321 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7381 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7441 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7501 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7561 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7621 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7681 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7741 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7801 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7861 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7921 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7981 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8041 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8101 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8161 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8221 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8281 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8341 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8401 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8461 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8521 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8581 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8641 AATTTT
//