LOCUS       MR226035                9294 bp    DNA     circular  SYN 
DEFINITION  Magi1 (Myc-DDK-tagged) - Mouse membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant 3
ACCESSION   NM_001029850
VERSION     NM_001029850.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5451..5542 
                     /label="Myc-DDK"  
     misc_feature    5572..6157 
                     /label="PolyA signal"  
     misc_feature    5629..5648 
                     /label="Restriction Enzyme"  
     misc_feature    6433..7105 
                     /label="ColE1"  
     misc_feature    7535..8329 
                     /label="Kan/Neo"  
     misc_feature    8364..8666 
                     /label="SV40 ori"  
     misc_feature    8752..9229 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5433..5439 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5433 
                     /label="Magi1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGAAAGTG ATCCAGAAGA AGAACCACTG GACTGGCCGC GTTCACGAGT 
     1081 GCACCGTGAA GCGGGGACCC CAGGGCGAGC TGGGGGTGAC GGTCCTGGGG GGCGCGGAGC 
     1141 ATGGGGAGTT TCCGTACGTG GGGGCGGTGG CGGCGGCCGA GGCGGCGGGG CTTCCCGGCG 
     1201 GTGGCGAGGG GCCGAAGCTG GCCGAAGGTG AGCTGCTGCT GGAGGTGCAG GGGGTCCGGG 
     1261 TGTCCGGCTT GCCCCGCTAT GACGTGCTGG GAGTCATCGA CAGCTGCAAG GAGGCCGTCA 
     1321 CCTTCAAAGC CGTCAGACAA GGAGGAAGGC TCAACAAGGA CCTACGACAT TTCCTCAACC 
     1381 AACGGTTCCA GAAGGGGTCT CCAGATCATG AGCTCCAGCA GACCATAAGG GACAACCTCT 
     1441 ACCGCCATGC TGTGCCTTGC ACAACCCGGT CTCCCAGAGA AGGAGAAGTG CCTGGTGTGG 
     1501 ATTACAGCTT TCTGACTGTG AAGGAGTTCT TGGACCTCGA GCAGAGCGGG ACCCTGTTGG 
     1561 AAGTCGGCAC CTATGAAGGA AACTATTATG GGACACCCAA ACCTCCTAGC CAGCCAGTCA 
     1621 GTGGGAAAGT GATCACGACG GATGCCTTGC ACAGCCTGCA GTCTGGCTCC AAGCAGTCGA 
     1681 CCCCTAAGCG AACAAAGTCC TACAATGATA TGCAAAATGC TGGCATAGTC CACCCGGAGA 
     1741 ATGAGGAGGA GGAGGATGTC CCTGAAATGA ACAGTAGCTT TACAGCCGAC TCTGGAGACC 
     1801 AGGACGAGCA CACTCTCCAA GAAGCAACGC TCCCGCCTGT GAATAGTAGC ATCCTCGCTG 
     1861 CTCCCATCAC GGACCCTTCT CAGAAGTTCC CTCAGTACCT ACCTCTTTCT GCAGAGGATA 
     1921 ATTTAGGTCC TCTACCTGAA AACTGGGAGA TGGCCTATAC TGAAAATGGA GAAGTCTATT 
     1981 TCATAGACCA CAACACGAAA ACAACATCAT GGTTAGACCC TCGGTGCCTG AACAAACAGC 
     2041 AGAAGCCTCT GGAAGAATGT GAAGATGATG AAGGGGTACA CACCGAGGAG CTGGACAGTG 
     2101 AACTAGAGTT GCCTGCTGGC TGGGAAAAGA TTGAAGACCC TGTCTACGGT GTCTACTATG 
     2161 TAGACCACAT CAACAGGAAG ACGCAATATG AAAACCCAGT CCTAGAAGCC AAACGGAAGA 
     2221 AACAGCTTGA ACAGCAGCAG CAACAGCAGC AGCCTCAGCC ACCGCAGCCA GAAGAGTGGA 
     2281 CAGAGGATCA TGCATCTGTT GTGCCTCCTG TTGCTCCTTC CCATCCCCCG AGCAATCCGG 
     2341 AGCCAGCCAG GGAAACTCCA CTTCAGGGCA AACCTTTTTT TACAAGAAAC CCCTCTGAGC 
     2401 TGAAAGGCAA GTTCATTCAC ACGAAGCTAC GGAAAAGCAG CCGAGGCTTT GGCTTCACGG 
     2461 TGGTTGGAGG AGACGAGCCT GATGAGTTCC TGCAGATCAA GAGCCTCGTC CTCGATGGTC 
     2521 CTGCCGCACT GGATGGCAAG ATGGAGACAG GGGATGTAAT TGTGAGTGTG AATGACACCT 
     2581 GTGTTTTGGG ACACACACAT GCTCAAGTTG TGAAAATCTT CCAGTCCATT CCCATTGGTG 
     2641 CCAGTGTGGA CCTTGAACTC TGCAGAGGTT ATCCATTGCC TTTTGACCCG GATGACCCTA 
     2701 ATACAAGTTT AGTGACCTCG GTGGCCATTT TGGACAAAGA ACCAATTATT GTAAATGGAC 
     2761 AAGAGACCTA CGATTCACCA GCGAGCCACA GTAGTAAAAC AGGCAAAGTC AGCAGCATGA 
     2821 AGGATGCCAG GCCAAGCAGC CCTGCTGATG TGGCTTCCAA CAGCTCTCAT GGTTATCCCA 
     2881 ACGACACAGT CTCCTTGGCT TCCTCCATAG CCACCCAGCC AGAGCTAATA ACTGTTCACA 
     2941 TAGTCAAAGG GCCAATGGGA TTTGGCTTTA CGATCGCAGA CAGTCCCGGT GGGGGTGGCC 
     3001 AAAGAGTGAA ACAGATTGTT GACAGTCCAC GCTGCAGAGG CCTCAAAGAA GGGGATCTTA 
     3061 TCGTGGAGGT GAATAAGAAG AACGTGCAGG CCCTGACGCA CAATCAAGTC GTGGATATGC 
     3121 TGATTGAATG TCCCAAGGGA AGTGAGGTCA CACTGTTGGT GCAGCGAGGA GGGCTACCAG 
     3181 TTCCCAAGAA GAGCCCAAAG TCGCAGCCAC TGGAGAGGAA AGACAGCCAG AATAGCTCCC 
     3241 AGCACAGCGT CTCCAGCCAC CGGAGCCTGC ACACTGCGTC CCCGAGCCAC GGCATACAGG 
     3301 TGCTCCCTGA GTACCTACCT GCAGACGCCC CTGCTCCAGA TCAGACCGAC AGCTCTGGGC 
     3361 AGAAAAAGCC AGATCCTTTT AAAATCTGGG CCCAGTCCAG GAGCATGTAT GAAAACCGAC 
     3421 CTATGTCACC TTCGCCTGCA TCAGGATTGA GCAAGGGTGA AAGAGACAGA GAAATCAATT 
     3481 CCACGAATTT TGGAGAATGT CAGATTCCAG ATTACCAGGA ACAGGACATC TTCCTCTGGA 
     3541 GAAAAGAAAC CGGATTTGGA TTTAGGATTC TGGGTGGAAA TGAACCAGGG GAACCCATTT 
     3601 ATATCGGTCA CATCGTACCG CTGGGTGCTG CTGACACAGA CGGCCGCCTG AGGTCTGGAG 
     3661 ATGAATTAAT CTGTGTGGAT GGGACACCAG TAATTGGGAA ATCACACCAG CTCGTGGTCC 
     3721 AGCTTATGCA ACAAGCTGCC AAGCAAGGCC ATGTCAATCT CACAGTGAGG CGGAAAGTGG 
     3781 TCTTTGCCGT CCCCAAAGCA GAGAATGAGG TGCCCTCACC AGCCTCATCA CACCACAGTA 
     3841 GCAACCAGCC CGCGTCCCTG ACGGAGGAGA AACGCACACC GCAAGGCAGC CAGAACTCTC 
     3901 TGAACACTGT GAGCTCTGGC AGCGGCAGCA CCAGTGGCAT TGGCAGTGGT GGCGGCGGGG 
     3961 GCAGCGGTGT GGTGAGCGCT GTGCTGCAGC CCTATGATGT GGAGATTCGG CGTGGGGAGA 
     4021 ACGAGGGCTT TGGGTTTGTC ATCGTGTCCT CCGTGAGCAG ACCCGAAGCG GGCACAACCT 
     4081 TCGGTCGGAT TATTGAGGGG AGCCCTGCTG ACCGCTGTGG CAAGCTGAAA GTAGGAGACC 
     4141 GGATCTTGGC AGTAAATGGA TGTTCCATCA CCAACAAATC CCATTCTGAC ATTGTCAACC 
     4201 TAATCAAAGA AGCGGGCAAC ACAGTGACTC TCCGCATCAT CCCCGGGGAT GAGTCCTCAA 
     4261 ATGCCACGCT GCTGACTAAT GCTGAGAAGA TTGCCACCAT CACCACCACT CATGCCCCCT 
     4321 CTCAGCAGGG GACCCAGGAA ACAAGGACCA CCACCAAACC AAAGCAGGAT TCTCAGTTTG 
     4381 AGTTCAAAGG ACCGCAGGCT GCACAGGAGC AAGATTTCTA CACTGTGGAA TTGGAAAGAG 
     4441 GGGCCAAGGG ATTTGGCTTT AGTCTTCGAG GGGGCCGAGA ATATAACATG GATCTTTATG 
     4501 TTCTGCGCTT GGCAGAGGAT GGTCCTGCAG AAAGATGTGG GAAGATGAGG ATTGGCGATG 
     4561 AAATTCTAGA GATCAATGGT GAGACCACCA AAAACATGAA ACACTCTCGG GCCATAGAAC 
     4621 TGATCAAGAA TGGCGGCCGC AGGGTCCGTC TGTTTCTGCG GCGGGGAGAC GGCTCAGTCC 
     4681 CAGAATATGA CCCCAGCAGC GACAGGAACG GCCCCTCCAC CGGTGCACAA GGTGTTCCGG 
     4741 AAGTGAGGCC GGGGCCGCCA GACCACAGAC CGCATCCAGC CTTGGAGTCC AGTTACCCAC 
     4801 CCGAACTTCA CAAATCATCA CAACATGCCG AAAAGCGAGC ACACGCGAAG GATCCAAAAG 
     4861 GCAACAGGGA GCACAGCAAA CAACCCAACG AACATCACAC CTGGAATGGA ACTTCTAGAA 
     4921 AACAGGACAG CGGGGCATGC CGGCCCAAAG ACCGGCCACC CGACGCATGG AGAGAGGCGC 
     4981 AGCCAGAGCG GACAGCCACC AATGGTTCAA AGAGGAGGTC ACCGGAGAAG CGCAGGGAAG 
     5041 GCACCCGCAG CGCTGACAAC ACTTTGGAAA GAAGGGAGAA GCATGAGAAG AGAAGAGAGA 
     5101 TATCTCCCGA GAGGAAGCGA GAGCGTTCAC CCACCCGGAG AAAAGATAGC TCCCCGAGCC 
     5161 GCCGGCGGAG GTCCCTCGAA AGACTCCTGG ATCAAAGACG GTCCCCAGAG CGCAGAAGAG 
     5221 GGGGCTCCCC CGAGAGGAGA GCCAAATCCA CCGACAGGAG GAGGGCCAGG TCCCCTGAGC 
     5281 GCAGGCGAGA GCGGTCACTG GACAAAAGGA ACCGGGACGA CAAGGTTGGC CACCGAGAAA 
     5341 GAGAGGAGGC TGGTCTGAAG CTGGAAGCGG GGAGAAGCCC CCGAAATCCC CCAGAGCAGA 
     5401 GAAGGCGGCC TTACAAAGAA TGTAGCACCG ACCTCAGCAT CACGCGTACG CGGCCGCTCG 
     5461 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5521 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5581 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5641 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5701 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5761 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5821 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5881 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5941 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     6001 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     6061 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     6121 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     6181 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     6241 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     6301 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     6361 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6421 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6481 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6541 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6601 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6661 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6721 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6781 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6841 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6901 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6961 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     7021 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     7081 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     7141 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     7201 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     7261 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     7321 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     7381 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7441 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7501 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7561 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7621 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7681 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7741 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7801 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7861 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7921 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7981 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     8041 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     8101 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     8161 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     8221 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     8281 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     8341 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     8401 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8461 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8521 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8581 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8641 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8701 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8761 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8821 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8881 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8941 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     9001 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     9061 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     9121 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     9181 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     9241 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//