LOCUS       MR226034                8394 bp    DNA     circular  SYN 
DEFINITION  Magi1 (Myc-DDK-tagged) - Mouse membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant 1
ACCESSION   NM_010367
VERSION     NM_010367.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4551..4642 
                     /label="Myc-DDK"  
     misc_feature    4672..5257 
                     /label="PolyA signal"  
     misc_feature    4729..4748 
                     /label="Restriction Enzyme"  
     misc_feature    5533..6205 
                     /label="ColE1"  
     misc_feature    6635..7429 
                     /label="Kan/Neo"  
     misc_feature    7464..7766 
                     /label="SV40 ori"  
     misc_feature    7852..8329 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4533..4539 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4533 
                     /label="Magi1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGAAAGTG ATCCAGAAGA AGAACCACTG GACTGGCCGC GTTCACGAGT 
     1081 GCACCGTGAA GCGGGGACCC CAGGGCGAGC TGGGGGTGAC GGTCCTGGGG GGCGCGGAGC 
     1141 ATGGGGAGTT TCCGTACGTG GGGGCGGTGG CGGCGGCCGA GGCGGCGGGG CTTCCCGGCG 
     1201 GTGGCGAGGG GCCGAAGCTG GCCGAAGGTG AGCTGCTGCT GGAGGTGCAG GGGGTCCGGG 
     1261 TGTCCGGCTT GCCCCGCTAT GACGTGCTGG GAGTCATCGA CAGCTGCAAG GAGGCCGTCA 
     1321 CCTTCAAAGC CGTCAGACAA GGAGGAAGGC TCAACAAGGA CCTACGACAT TTCCTCAACC 
     1381 AACGGTTCCA GAAGGGGTCT CCAGATCATG AGCTCCAGCA GACCATAAGG GACAACCTCT 
     1441 ACCGCCATGC TGTGCCTTGC ACAACCCGGT CTCCCAGAGA AGGAGAAGTG CCTGGTGTGG 
     1501 ATTACAGCTT TCTGACTGTG AAGGAGTTCT TGGACCTCGA GCAGAGCGGG ACCCTGTTGG 
     1561 AAGTCGGCAC CTATGAAGGA AACTATTATG GGACACCCAA ACCTCCTAGC CAGCCAGTCA 
     1621 GTGGGAAAGT GATCACGACG GATGCCTTGC ACAGCCTGCA GTCTGGCTCC AAGCAGTCGA 
     1681 CCCCTAAGCG AACAAAGTCC TACAATGATA TGCAAAATGC TGGCATAGTC CACCCGGAGA 
     1741 ATGAGGAGGA GGAGGATGTC CCTGAAATGA ACAGTAGCTT TACAGCCGAC TCTGGAGACC 
     1801 AGGACGAGCA CACTCTCCAA GAAGCAACGC TCCCGCCTGT GAATAGTAGC ATCCTCGCTG 
     1861 CTCCCATCAC GGACCCTTCT CAGAAGTTCC CTCAGTACCT ACCTCTTTCT GCAGAGGATA 
     1921 ATTTAGGTCC TCTACCTGAA AACTGGGAGA TGGCCTATAC TGAAAATGGA GAAGTCTATT 
     1981 TCATAGACCA CAACACGAAA ACAACATCAT GGTTAGACCC TCGGTGCCTG AACAAACAGC 
     2041 AGAAGCCTCT GGAAGAATGT GAAGATGATG AGTTGCCTGC TGGCTGGGAA AAGATTGAAG 
     2101 ACCCTGTCTA CGGTGTCTAC TATGTAGACC ACATCAACAG GAAGACGCAA TATGAAAACC 
     2161 CAGTCCTAGA AGCCAAACGG AAGAAACAGC TTGAACAGCA GCAGCAACAG CAGCAGCCTC 
     2221 AGCCACCGCA GCCAGAAGAG TGGACAGAGG ATCATGCATC TGTTGTGCCT CCTGTTGCTC 
     2281 CTTCCCATCC CCCGAGCAAT CCGGAGCCAG CCAGGGAAAC TCCACTTCAG GGCAAACCTT 
     2341 TTTTTACAAG AAACCCCTCT GAGCTGAAAG GCAAGTTCAT TCACACGAAG CTACGGAAAA 
     2401 GCAGCCGAGG CTTTGGCTTC ACGGTGGTTG GAGGAGACGA GCCTGATGAG TTCCTGCAGA 
     2461 TCAAGAGCCT CGTCCTCGAT GGTCCTGCCG CACTGGATGG CAAGATGGAG ACAGGGGATG 
     2521 TAATTGTGAG TGTGAATGAC ACCTGTGTTT TGGGACACAC ACATGCTCAA GTTGTGAAAA 
     2581 TCTTCCAGTC CATTCCCATT GGTGCCAGTG TGGACCTTGA ACTCTGCAGA GGTTATCCAT 
     2641 TGCCTTTTGA CCCGGATGAC CCTAATACAA GTTTAGTGAC CTCGGTGGCC ATTTTGGACA 
     2701 AAGAACCAAT TATTGTAAAT GGACAAGAGA CCTACGATTC ACCAGCGAGC CACAGTAGTA 
     2761 AAACAGGCAA AGTCAGCAGC ATGAAGGATG CCAGGCCAAG CAGCCCTGCT GATGTGGCTT 
     2821 CCAACAGCTC TCATGGTTAT CCCAACGACA CAGTCTCCTT GGCTTCCTCC ATAGCCACCC 
     2881 AGCCAGAGCT AATAACTGTT CACATAGTCA AAGGGCCAAT GGGATTTGGC TTTACGATCG 
     2941 CAGACAGTCC CGGTGGGGGT GGCCAAAGAG TGAAACAGAT TGTTGACAGT CCACGCTGCA 
     3001 GAGGCCTCAA AGAAGGGGAT CTTATCGTGG AGGTGAATAA GAAGAACGTG CAGGCCCTGA 
     3061 CGCACAATCA AGTCGTGGAT ATGCTGATTG AATGTCCCAA GGGAAGTGAG GTCACACTGT 
     3121 TGGTGCAGCG AGGAGGGCTA CCAGTTCCCA AGAAGAGCCC AAAGTCGCCA CTGGAGAGGA 
     3181 AAGACAGCCA GAATAGCTCC CAGCACAGCG TCTCCAGCCA CCGGAGCCTG CACACTGCGT 
     3241 CCCCGAGCCA CGGCATACAG GTGCTCCCTG AGTACCTACC TGCAGACGCC CCTGCTCCAG 
     3301 ATCAGACCGA CAGCTCTGGG CAGAAAAAGC CAGATCCTTT TAAAATCTGG GCCCAGTCCA 
     3361 GGAGCATGTA TGAAAACCGA CTTCCAGATT ACCAGGAACA GGACATCTTC CTCTGGAGAA 
     3421 AAGAAACCGG ATTTGGATTT AGGATTCTGG GTGGAAATGA ACCAGGGGAA CCCATTTATA 
     3481 TCGGTCACAT CGTACCGCTG GGTGCTGCTG ACACAGACGG CCGCCTGAGG TCTGGAGATG 
     3541 AATTAATCTG TGTGGATGGG ACACCAGTAA TTGGGAAATC ACACCAGCTC GTGGTCCAGC 
     3601 TTATGCAACA AGCTGCCAAG CAAGGCCATG TCAATCTCAC AGTGAGGCGG AAAGTGGTCT 
     3661 TTGCCGTCCC CAAAGCAGAG AATGAGGTGC CCTCACCAGC CTCATCACAC CACAGTAGCA 
     3721 ACCAGCCCGC GTCCCTGACG GAGGAGAAAC GCACACCGCA AGGCAGCCAG AACTCTCTGA 
     3781 ACACTGTGAG CTCTGGCAGC GGCAGCACCA GTGGCATTGG CAGTGGTGGC GGCGGGGGCA 
     3841 GCGGTGTGGT GAGCGCTGTG CTGCAGCCCT ATGATGTGGA GATTCGGCGT GGGGAGAACG 
     3901 AGGGCTTTGG GTTTGTCATC GTGTCCTCCG TGAGCAGACC CGAAGCGGGC ACAACCTTCG 
     3961 AGTCCTCAAA TGCCACGCTG CTGACTAATG CTGAGAAGAT TGCCACCATC ACCACCACTC 
     4021 ATGCCCCCTC TCAGCAGGGG ACCCAGGAAA CAAGGACCAC CACCAAACCA AAGCAGGATT 
     4081 CTCAGTTTGA GTTCAAAGGA CCGCAGGCTG CACAGGAGCA AGATTTCTAC ACTGTGGAAT 
     4141 TGGAAAGAGG GGCCAAGGGA TTTGGCTTTA GTCTTCGAGG GGGCCGAGAA TATAACATGG 
     4201 ATCTTTATGT TCTGCGCTTG GCAGAGGATG GTCCTGCAGA AAGATGTGGG AAGATGAGGA 
     4261 TTGGCGATGA AATTCTAGAG ATCAATGGTG AGACCACCAA AAACATGAAA CACTCTCGGG 
     4321 CCATAGAACT GATCAAGAAT GGCGGCCGCA GGGTCCGTCT GTTTCTGCGG CGGGGAGACG 
     4381 GCTCAGTCCC AGAATATGCG ATGATCCCTC CTAAAATCGC TGCATGTATG AGAAATGAAA 
     4441 AGCTCGGGGA GGCTTGCTTC TACCTTATGG GCCATAATCA AACTACGACC CCAGCAGCGA 
     4501 CAGGAACGGC CCCTCCACCG GTGCACAAGG TGTTCCGGAA GACGCGTACG CGGCCGCTCG 
     4561 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4621 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4681 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4741 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4801 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4861 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4921 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4981 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5041 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5101 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5161 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5221 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5281 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5341 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5401 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5461 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5521 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5581 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5641 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5701 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5761 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5821 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5881 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5941 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6001 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6061 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6121 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6181 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6241 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6301 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6361 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6421 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6481 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6541 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6601 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6661 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6721 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6781 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6841 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6901 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6961 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7021 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7081 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7141 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7201 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7261 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7321 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7381 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7441 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7501 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7561 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7621 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7681 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7741 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7801 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7861 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7921 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7981 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8041 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8101 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8161 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8221 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8281 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8341 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//