LOCUS       MR226021                8271 bp    DNA     circular  SYN 
DEFINITION  Srebf2 (Myc-DDK-tagged) - Mouse sterol regulatory element binding factor 2 (Srebf2)
ACCESSION   NM_033218
VERSION     NM_033218.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4428..4519 
                     /label="Myc-DDK"  
     misc_feature    4549..5134 
                     /label="PolyA signal"  
     misc_feature    4606..4625 
                     /label="Restriction Enzyme"  
     misc_feature    5410..6082 
                     /label="ColE1"  
     misc_feature    6512..7306 
                     /label="Kan/Neo"  
     misc_feature    7341..7643 
                     /label="SV40 ori"  
     misc_feature    7729..8206 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4410..4416 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4410 
                     /label="Srebf2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGAGAGC AGCGAGCTGG GCGTTCTGGA GACCATGGAG ACCCTCACGG 
     1081 AGCTGGGCGA TGAGCTGACT CTCGGGGACA TCGACGAGAT GCTACAGTTT GTCAGCAATC 
     1141 AAGTGGGAGA GTTCCCTGAT TTGTTTTCAG AGCAACTTTG TAGCTCCTTC CCTGGAGGTG 
     1201 GCAGCAATGG TGGCAGTGGC AACAACAGCA GTGGCAGAGG CAACAATGGT GGAGCCACAG 
     1261 ACCCTGCAGT ACAGCGGTCA TTCAGCCAGG TCCCATTATC TACCTTCTCT CCCTCAGCTG 
     1321 CCTCCCCTCA GGCTCCGGCC CTGCAGGTCA AAGTCTCTCC TACCCCACCA AGGGCAACCC 
     1381 CTGTTCTCCA GCCGCGACCC CAGCCCCAGC CCCAACCTCC AGCTCAGCTG CAGCAGCAGA 
     1441 CGGTGATGAT CACCCCGACG TTCAGCACCG CTCCGCAGAC GAGGATCATC CAGCAGCCTT 
     1501 TGATATACCA GAACGCAGCG ACCAGCTTTC AAGTCCTGCA GCCTCAAGTG CAAAGCCTCG 
     1561 TGACGTCCCC GCAGGTGCAG CCCGTCACCA TCCAGCAGCA GGTGCAGACG GTACAGGCCC 
     1621 AGCGGGTGTT GACGCAGACA GCCAATGGCA CACTGCAGAC CCTTGCCCCC GCCACGGTAC 
     1681 AGACGGTCGC TGCGCCACAG GTGCAGCAGG TCCCGGTCCT GGTGCAACCT CAGATCATTA 
     1741 AGACAGATTC CCTTGTGTTG ACCACGCTGA AGACAGATGG CAGCCCTGTC ATGGCTGCAG 
     1801 TCCAGAACCC GGCCCTCACG GCCCTCACTG CCCCCATCCA GACAGCCGCC CTTCAAGTGC 
     1861 CAACCCTGGT GGGCAGCAAC GGGACCATTC TGACCACAAT GCCTGTGATG ATGGGGCAAG 
     1921 AGAAAGTTCC TATCAAGCAA GTGCCTGGCG GCGTGAAGCA GCTGGATCCT CCCAAAGAAG 
     1981 GAGAGAGGCG GACAACACAC AATATCATTG AAAAGCGCTA CCGGTCCTCC ATCAACGACA 
     2041 AAATCATAGA GTTGAAGGAC TTAGTCATGG GGACAGATGC CAAGATGCAC AAGTCTGGCG 
     2101 TTCTGAGGAA GGCCATTGAT TACATCAAAT ATCTGCAGCA GGTCAATCAC AAGCTGCGCC 
     2161 AGGAGAACAT GGTGCTGAAG CTGGCCAATC AGAAAAACAA GCTCCTGAAG GGCATCGACC 
     2221 TGGGCAGTCT GGTGGACAGT GATGTGGACT TGAAAATTGA TGACTTTAAC CAGAATGTCC 
     2281 TTCTGATGTC TCCGCCGGCC TCCGACTCCG GGTCCCAGGC CGGCTTCTCT CCCTATTCCA 
     2341 TTGACTCTGA GCCGGGCAGC CCTCTGCTGG ATGACGCAAA GGTCAAGGAT GAACCGGACT 
     2401 CTCCTCCTGT GGCACTGGGC ATGGTGGACC GCTCTCGAAT CCTCTTATGT GTCCTCACCT 
     2461 TCCTGGGCCT CTCCTTTAAC CCCTTGACTT CCTTGCTGCA GTGGGGAGGG GCCCACAACA 
     2521 CTGACCAGCA CCCATACTCA GGCTCGGGCC GCAGTGTACT GTCACTGGAG TCAGGTGCTG 
     2581 GGGGCTGGTT TGACTGGATG GTTCCAACTC TCCTCCTGTG GCTGGTAAAT GGTGTGATTG 
     2641 TCTTGAGCGT CTTTGTGAAG CTGTTGGTCC ACGGGGAGCC GGTGATTCGC CCACACTCAC 
     2701 GCCCCTCAGT CACCTTCTGG AGACACCGGA AGCAGGCAGA CCTAGACCTC GCCAAAGGTG 
     2761 ATTTCGCAGC TGCTGCTGCC AACCTACAAA CCTGCTTATC CGTGCTGGGC CGGGCGCTGC 
     2821 CCACCTCCCG CCTGGACTTG GCCTGCAGCC TCTCCTGGAA TGTGATCCGC TACAGCCTGC 
     2881 AGAAGCTGCG CCTGGTACGC TGGTTACTCA AGAAGGTCTT CCAGCGCTGG CGGGCTACTC 
     2941 CCGCCACTGC AGCTGGCTTT GAGGATGAGG CTAAGAGCAG CGCAAGGGAT GCGGCACTGG 
     3001 CCTACCACAG GCTGCACCAG CTGCACATCA CAGGGAAGCT TCCGGCGGGA TCTGCCTGTT 
     3061 CAGATGTGCA CATGGCTCTG TGTGCTGTGA ACCTGGCCGA GTGTGCGGAG GAGAAAATCC 
     3121 TACCAAGCAC ACTGATTGAG ATCCACCTGA CAGCGGCCAT GGGGCTCAAG ACCCGCTGTG 
     3181 GAGGCAAGCT GGGCTTCTTG GCTAGCTACT TCTTAAATCG TGCCCAGAGT CTGTGTGGCC 
     3241 CAGAGCATAG CACTGTCCCC GACTCCCTGC GCTGGCTATG CCATCCCTTG GGCCAGAAGT 
     3301 TTTTCATGGA GCGAAGCTGG TCGATCAAGT CAGCAGCCAA GGAGAGCCTG TACTGCGCCC 
     3361 AGAGGAGCCC AGCTGACCCC ATTGCCCAGG TACACCAGGC GTTCTGCAAA AACCTGCTAG 
     3421 AAAGAGCGGT GGAGTCCTTG GTGAAACCTC AGGCCAAGAA GAAGGCAGGC GACCAGGAAG 
     3481 AAGAGAGCTG TGAATTTTCC AGTGCTCTAG AGTATTTGAA ATTACTTCAT TCTTTTGTGG 
     3541 ACTCTGTGGG ATTTGTGACC TCACCATTCT CCAGCAGTTC CGTGCTCAGG TCGGCCCTTG 
     3601 GCCCCGATGT CATCTGCCGG TGGTGGACGT CCGCAGTCAC CATGGCCATC AGCTGGCTCC 
     3661 AGGGAGACGA TGCAGCTGTG CGCTCTCGTT TTACTGAAGT AGAGCGGGTC CCCAAGGCCC 
     3721 TGGAAGTGAC CGAGAGTCCC TTGGTGAAGG CCGTCTTCTA TACCTGTAGA GCCATGCATG 
     3781 CCTCCCTCTC TGGGAAAGCT GACGGGCAGC AGAACTCCTT CTGTCACTGT GAGAGGGCCA 
     3841 GTGGTCACCT GTGGAGCAGT CTCAACGTCA GCGGCACTAC TTCTGATCCT TCACTTAACC 
     3901 ATGTGATCCA GCTGTTCACC TGTGACCTGC TACTGTCGCT GAGGACAGCA CTCTGGCAAA 
     3961 AGCAGGCCAG TGCCAGCCAG CTCCTGGGTG AGACCTACCA TGCATCCGGC ACTGAGCTGG 
     4021 CCGGCTTCCA GCGGGACCTG GGCAGCCTTC GAAGGCTGGC CCATAGCTTC CGCCCAGCCT 
     4081 ACCGCAAGGT GTTCCTGCAT GAAGCCACTG TGCGCCTGAT GGCAGGAGCC AGCCCTACCC 
     4141 GTACACACCA GCTGCTGGAG CATAGCCTAC GGCGGCGCCC CACTCAGAAC ACCAAGCATG 
     4201 GAGAGGTAGA CACCTGGCCT GGCCAACGAG AGCGAGCTAC TGCCATCCTG CTGGCCTGCC 
     4261 GCCATCTTCC CCTCTCTTTC CTGTCTTCCC CGGGCCAGCG GGCAGTGCTG CTGGCTGAAG 
     4321 CTGCCCGCAC CCTGGAGAAG GTGGGTGACC GCCGTTCCTG CAGTGACTGC CAGCAGATGA 
     4381 TTGTCAAGCT GGGCGGTGGC ACTGCCATCG CTGCCTCCAC GCGTACGCGG CCGCTCGAGC 
     4441 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4501 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4561 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4621 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4681 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4741 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4801 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4861 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4921 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4981 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5041 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5101 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5161 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5221 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5281 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5341 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5401 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5461 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5521 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5581 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5641 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5701 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5761 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5821 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5881 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5941 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6001 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6061 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6121 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6181 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6241 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6301 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6361 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6421 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6481 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6541 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6601 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6661 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6721 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6781 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6841 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6901 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6961 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7021 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7081 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7141 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7201 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7261 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7321 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7381 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7441 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7501 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7561 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7621 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7681 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7741 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7801 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7861 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7921 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7981 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8041 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8101 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8161 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8221 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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