LOCUS       MR225979                8856 bp    DNA     circular  SYN 
DEFINITION  Cngb1 (Myc-DDK-tagged) - Mouse cyclic nucleotide gated channel beta 1 (Cngb1), transcript variant 1
ACCESSION   NM_001195413
VERSION     NM_001195413.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5013..5104 
                     /label="Myc-DDK"  
     misc_feature    5134..5719 
                     /label="PolyA signal"  
     misc_feature    5191..5210 
                     /label="Restriction Enzyme"  
     misc_feature    5995..6667 
                     /label="ColE1"  
     misc_feature    7097..7891 
                     /label="Kan/Neo"  
     misc_feature    7926..8228 
                     /label="SV40 ori"  
     misc_feature    8314..8791 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4995..5001 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4995 
                     /label="Cngb1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGGGCTGG GTCCAAAGGG TGCTGCCTCA GCCTCCGGGG ACCCCTCAGA 
     1081 AGACCGTGGA GACAGCAGGA CCACAGCCAG AAACAGAGTC GAAACCAGAG GCCAACCCAC 
     1141 AACCGGAGCC AGAACCTCAG CAGGAACCAG AGCCAGAGCC AGAGCCAGAG CCAGAGCCAG 
     1201 AGCCAGAGCC AGAGCCAGAG CCAGAGCCAG AGCCAGAACC TGAACCTGAA CCAGTACCTG 
     1261 AAGAGGCTCC ACCTGAGGTC CAGGCCCTGC CACCAGAGGA GCCAATGGAA GGAGAGGGAG 
     1321 AGGCTGAGGC TGGTCCCAGC CTTCAAGAGA CCCAGGTAGC TGACCCTGCT CAGCCCACCT 
     1381 CCCAGGCCCA GGTTGCTGTT GCCAAGGTGA ACAGGCCCAG CTCCTGGATG TTGAGCTGGT 
     1441 TCTGGAGAGG CATGCAGAAG GTCGTGCCAC AGCCTGTCTG CAGCAATGGG GGCCAGAACC 
     1501 TGGCTGCCGG AGAGCGAGAC CCAGATCAGG GTGGAGCACA GATCCCAGAA CCTTGTGGCA 
     1561 CTGGGGACCC AGGATCTGCA GAAGCTTCCG GGACTCAGGA CACTGAGCCC AGCCTTTGGC 
     1621 TGCTCAGGTG GCTTGAGCAG AATCTGGAAA AAGTGCTGCC CCAGCCCCCT CCGCCTTCCC 
     1681 TGGCCTGGAA AGTTGAACCT GAGGCTGCTG TCTTGGATCC AGATCCTCCA GGAACCCCTA 
     1741 TGCAGATGGA GCCCACAGAG AGCCCCTCCC AGCCTAATCC TGGACCCCTG GAGCCTGAGG 
     1801 AAGAGCCAGC TGCAGAGCCC CAGCCTGGCT TCCAATCCTC TTCCCTGCCA CCACCCGGGG 
     1861 ACCCCGTCAG GCTGATTGAG TGGCTCCTAC ACAGACTGGA GATGGCCCTG CCTCAGCCCG 
     1921 TGCTCCATGG GAAGGCTGCA GAGCAAGAGC CTGGCTGCCC TGGGATGTGT GACGTACAGA 
     1981 CCATCAGCAT CCTCCCCGTG GAACAGGTGG AACATGATCT TGTCCTGGAG GAGGTGGACT 
     2041 CTTGCTGGGA GGATGCTCAG CAGGAAGATG GCGCCAGCCC GCAGGAGACA GAGGTGGCTC 
     2101 CAGCTCATGA AGAAGAGAGT GAGGCCATAG TGGAGATACC CAGGGAGCTG ACGAAGATTC 
     2161 AAGAAGAAAG AGAAGATGAG CAGGAAGAGG ATGAGGAAGA GAAGGAGGAA GAGAAGAAGA 
     2221 AGGGGGAGGA AGAGAAGGAG AAGGAGGAGG AAGAGAAAGA GAAAGAGAAG GAGAAGGAGG 
     2281 AGGAGAAGGA AGAGGAAGAG AAGGAGGAGG AGGAGGAGGA AGAAAAGGAA GAGGAAGAGG 
     2341 AGGAAAAGGA AGAAGAGGAG AAGGAAGAGG AGGAGAAGGA AGAGGAGGAA GAAGAAGAGG 
     2401 AGGAGGAAGA AGAGCCTATT GTCCTGCTGG ATAGCTGTTT GGTGGTGCAG GCTGATGTGG 
     2461 ACGAGTGCCA GCTAGAAAGG ACACCGTCAG AGCTAGCATC GATCCAGGAG CTGCCAGAAG 
     2521 AGAAAGAGGA GAAGGAAGAG GAGAAGGAGG AGGAGAAAGA GGAGGAGGAG GAAAAGAAGG 
     2581 AGGAAGAAGT GGAGAAGAAG GAGGAAGGGG AGGCCACAAA CTCAACAGTA CCAGCCACAA 
     2641 AGGAGCACCC GGAGCTCCAG GTAGAAGACA CGGATGCTGA TAGTGGCCCC CTCATCCCAG 
     2701 AGGAGACACT CCCTCCACCT GAGAGACCAC CACCGTCTCC TGTCAAGTCG GACACCCTCA 
     2761 CAGTTCCCGG CGCCGCTGCA GCAGGCCACA GGAAGAAGCT ACCTTCTCAG GATGATGAGG 
     2821 CCGAAGAGCT CAAGGCCCTG TCACCAGCTG AGTCCCCAGT GGTTGCCTGG TCGGACCCCA 
     2881 CCACCCCACA GGAGGCTGAT GGCCAGGACC GAGCAGCCTC CACCGCCAGC CAGAACAGTG 
     2941 CCATCATCAA TGACCGGCTC CAGGAGCTGG TGAAGATGTT CAAGGAGCGG ACGGAGAAGG 
     3001 TGAAGGAGAA GCTCATTGAC CCTGACGTCA CCTCTGATGA GGAGAGCCCC AAGCCCTCCC 
     3061 CGGCCAAGAA GGCCCCAGAA CCAGACCCAG CCCAGAAGCC AGCGGAGGCA GAGGTGGCAG 
     3121 AGGAGGAACA CTACTGCGAC ATGCTCTGCT GCAAGTTCAA GCGCAGGCCC TTGAAGATGT 
     3181 ACCGGTTCCC CCAGAGTATC GACCCACTGA CCAACCTCAT GTACATCTTG TGGCTGTTCT 
     3241 TTGTGGTGCT GGCCTGGAAC TGGAACTGCT GGCTGATCCC TGTGCGCTGG GCCTTCCCGT 
     3301 ACCAGCGGGC AGACAACATC CACTTCTGGC TCCTCATGGA TTACTTGTGC GACTTCATCT 
     3361 ACCTCCTGGA CATCACCGTG TTCCAGATGC GCCTGCAGTT TGTCAAAGGC GGGGACATCA 
     3421 TTACAGACAA GAAGGAGATG CGTAATAACT ACCTGAAGTC TCGCCGGTTT AAGATGGACC 
     3481 TGCTCTGCCT CCTGCCCTTG GACTTTCTCT ACTTGAAACT TGGCATCAAC CCTCTCCTTC 
     3541 GCCTGCCCCG CTGCCTGAAG TACATGGCCT TCTTTGAGTT TAATAACCGC CTGGAAGCCA 
     3601 TCCTCAGCAA AGCCTACGTT TACAGAGTCA TCAGGACCAC GGCCTACCTG CTGTACAGCC 
     3661 TGCACCTCAA CTCCTGCCTT TACTACTGGG CATCAGCCTT CCAGGGCATC GGCTCCACTC 
     3721 ACTGGGTTTA CGACGGAGTG GGGAACAGTT ATATTCGATG CTACTACTGG GCTGTGAAAA 
     3781 CCCTCATCAC CATCGGAGGA CTGCCCGACC CCCAGACGCT CTTTGAGATT GTCTTCCAGC 
     3841 TGCTGAACTA TTTTACCGGG GTCTTTGCTT TCTCTGTGAT GATTGGACAG ATGAGAGATG 
     3901 TGGTGGGGGC CGCCACAGCG GGGCAGACCT ACTACCGCAG CTGCATGGAC AGCACAGTGA 
     3961 AGTACATGAA CTTCTACAAG ATCCCCAGGT CTGTGCAGAA CCGTGTCAAG ACCTGGTATG 
     4021 AGTACACCTG GCATTCGCAA GGCATGCTGG ATGAGTCAGA GCTGATGGTA CAACTTCCGG 
     4081 ACAAGATGCG ACTGGACCTG GCCATTGATG TGAACTACAG TATTGTCAGC AAAGTGGCCC 
     4141 TCTTTCAGGG CTGTGACCGG CAGATGATCT TCGACATGCT CAAGCGGCTT CGTTCTGTTG 
     4201 TCTACCTACC CAATGACTAT GTGTGCAAGA AGGGGGAGAT TGGCCGAGAG ATGTACATTA 
     4261 TCCAGGCGGG GCAGGTGCAG GTGCTGGGCG GCCCAGATGG GAAGGCTGTA CTGGTGACAC 
     4321 TCAAAGCCGG ATCGGTGTTT GGAGAAATAA GCTTGCTGGC TGTCGGGGGT GGCAACCGGC 
     4381 GCACAGCCAA CGTGGTGGCC CATGGCTTCA CCAACCTCTT CATACTGGAC AAGAAGGACT 
     4441 TGAATGAAAT TTTAGTGCAT TACCCGGAAT CTCAGAAGTT ACTTCGGAAG AAGGCCAGGC 
     4501 GCATGCTAAG AAATAACAAC AAACCCAAGG AGGAAAAGAG TGTGCTCATC CTGCCCCCGC 
     4561 GTGCGGGCAC CCCGAAGCTC TTCAATGCTG CCCTGGCTGC TGCAGGAAAG ATGGGCCCCA 
     4621 GGGGAGCCAA GGGCGGCAAG CTCGCCCACC TGAGAGCCAG GCTCAAGGAA CTGGCTGCAC 
     4681 TGGAGGCAGC CGCGCGACAG CAACAGCTGC TGGAACAGGC CAAGAGCTCG CAAGAAGCTG 
     4741 GGGGAGAGGA GGGCTCTGGG GCCACAGACC AACCTGCACC CCAGGAGCCA CCAGAGCCCA 
     4801 AGGACCCTCC GAAGCCACCA GGGCCACCAG AGCCCTCAGC CCAGAGCTCT CCACCTCCAG 
     4861 CCTCAGCAAA GCCCGAGGAA AGCACGGGGG AGGCAGCAGG GCCCCCGGAG CCCTCGGTGA 
     4921 GGATCCGTGT GAGTCCAGGC CCTGATCCCG GGGAGCAGAC ACTGTCGGTG GAGGTGCTGG 
     4981 AGGAGAAGAA AGAGGGGGCG GAGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5041 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5101 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5161 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5221 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5281 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5341 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5401 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5461 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5521 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5581 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5641 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5701 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5761 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5821 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5881 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5941 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6001 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6061 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6121 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6181 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6241 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6301 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6361 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6421 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6481 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6541 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6601 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6661 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6721 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6781 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6841 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6901 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6961 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7021 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7081 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7141 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7201 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7261 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7321 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7381 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7441 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7501 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7561 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7621 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7681 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7741 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7801 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7861 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7921 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7981 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8041 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8101 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8161 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8221 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8281 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8341 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8401 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8461 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8521 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8581 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8641 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8701 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8761 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8821 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//