LOCUS       MR225933                8757 bp    DNA     circular  SYN 
DEFINITION  Erbb4 (Myc-DDK-tagged) - Mouse v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) (Erbb4)
ACCESSION   NM_010154
VERSION     NM_010154.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4914..5005 
                     /label="Myc-DDK"  
     misc_feature    5035..5620 
                     /label="PolyA signal"  
     misc_feature    5092..5111 
                     /label="Restriction Enzyme"  
     misc_feature    5896..6568 
                     /label="ColE1"  
     misc_feature    6998..7792 
                     /label="Kan/Neo"  
     misc_feature    7827..8129 
                     /label="SV40 ori"  
     misc_feature    8215..8692 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4896..4902 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4896 
                     /label="Erbb4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGCTGGCG ACGGGACTCT GGGTCTGGGG GAGCCTTCTG ATGGCAGCGG 
     1081 GGACCGTCCA GCCCAGCGCT TCTCAGTCAG TGTGCGCAGG AACAGAGAAC AAACTGAGCT 
     1141 CTCTCTCTGA CCTGGAACAG CAGTACCGAG CCTTGCGCAA ATACTATGAA AACTGCGAGG 
     1201 TAGTCATGGG CAACCTGGAG ATCACCAGCA TCGAGCACAA CCGGGACCTC TCCTTCCTGC 
     1261 GGTCTATCCG AGAAGTCACA GGCTACGTCC TGGTGGCCCT CAACCAGTTT CGTTACTTGC 
     1321 CTCTGGAGAA TTTACGCATT ATTCGTGGGA CAAAACTATA TGAAGATCGC TATGCCTTAG 
     1381 CGATATTCTT AAACTACAGG AAAGATGGCA ACTTTGGACT CCAAGAACTT GGATTAAAGA 
     1441 ACCTGACCGA AATACTAAAT GGTGGAGTCT ATGTAGACCA GAACAAATTC CTATGTTATG 
     1501 CTGACACTAT ACACTGGCAA GATATTGTTC GGAATCCATG GCCTTCCAAC ATGACTCTGG 
     1561 TGTCAACAAA TGGAAGTTCT GGATGTGGAA GATGCCATAA GTCTTGCACT GGCCGATGCT 
     1621 GGGGACCCAC AGAAAATCAC TGCCAGACCT TGACCAGAAC TGTGTGTGCT GAACAATGTG 
     1681 ATGGCAGGTG CTATGGACCC TACGTTAGTG ACTGCTGCCA TCGAGAATGT GCTGGAGGCT 
     1741 GCTCAGGACC AAAGGACACT GACTGCTTTG CCTGCATGAA CTTCAATGAC AGTGGAGCCT 
     1801 GCGTTACTCA ATGTCCCCAA ACATTTGTCT ACAATCCAAC CACCTTTCAA CTGGAACACA 
     1861 ACTTCAATGC AAAGTACACG TATGGAGCAT TCTGTGTTAA GAAATGTCCA CATAACTTCG 
     1921 TGGTAGATTC CAGTTCTTGT GTACGAGCCT GCCCTAGTTC TAAGATGGAA GTAGAAGAAA 
     1981 ATGGGATTAA AATGTGTAAG CCTTGCACCG ATATTTGCCC CAAAGCATGT GATGGAATCG 
     2041 GCACGGGATC ACTGATGTCT GCTCAGACTG TGGATTCAAG TAACATTGAC AAATTCATAA 
     2101 ACTGCACAAA GATCAATGGC AATCTCATCT TTCTTGTCAC TGGCATTCAT GGAGACCCTT 
     2161 ACAATGCTAT TGACGCCATA GATCCAGAGA AACTGAATGT CTTTCGGACT GTCAGAGAAA 
     2221 TAACAGGTTT CCTGAACATA CAGTCTTGGC CCCCAAATAT GACAGATTTC AGTGTTTTCT 
     2281 CCAACCTCGT CACAATTGGA GGAAGAGTCC TCTACAGTGG TCTCTCATTG CTGATCCTCA 
     2341 AACAACAAGG TATCACTTCC CTACAGTTCC AGTCTCTGAA GGAAATCAGT GCGGGCAATA 
     2401 TCTACATCAC TGACAACAGC AACCTGTGTT ATTACCATAC CATTAACTGG ACAACACTCT 
     2461 TCAGCACCAT TAACCAGAGA ATAGTGATCC GAGATAACAG AAGAGCTGAG AATTGTACTG 
     2521 CTGAAGGCAT GGTATGCAAC CACCTGTGTT CAAATGATGG TTGTTGGGGA CCTGGGCCGG 
     2581 ACCAGTGCCT GTCATGTCGG CGCTTCAGCA GGGGAAAGAT CTGCATAGAG TCTTGCAACC 
     2641 TTTATGATGG GGAATTTCGA GAGTTTGAAA ACGGCTCCAT CTGTGTTGAG TGTGACTCCC 
     2701 AGTGTGAGAA AATGGAAGAT GGACTCCTCA CATGCCATGG ACCGGGACCT GACAACTGCA 
     2761 CAAAGTGCTC TCATTTTAAG GATGGTCCAA ACTGTGTGGA GAAATGTCCA GATGGCCTAC 
     2821 AGGGAGCAAA CAGTTTCATT TTTAAGTATG CAGATCAGGA TCGGGAGTGC CACCCTTGCC 
     2881 ATCCAAACTG CACCCAGGGG TGTAACGGTC CCACTAGTCA TGACTGCATT TACTACCCAT 
     2941 GGACGGGCCA TTCCACTTTA CCACAACACG CTAGAACTCC ACTGATTGCA GCCGGAGTCA 
     3001 TTGGAGGCCT CTTCATCCTG GTGATCATGG CTTTGACATT TGCTGTCTAT GTCAGAAGAA 
     3061 AGAGCATCAA AAAGAAACGT GCTTTGAGGA GATTCCTGGA GACAGAGCTG GTAGAGCCCT 
     3121 TAACTCCCAG TGGCACGGCA CCCAATCAAG CTCAACTTCG CATTTTGAAG GAAACCGAAC 
     3181 TAAAGAGGGT AAAGGTCCTT GGCTCGGGAG CTTTTGGAAC CGTTTATAAA GGTATTTGGG 
     3241 TGCCTGAAGG TGAAACAGTG AAAATCCCTG TGGCTATAAA GATCCTCAAT GAAACAACTG 
     3301 GCCCCAAAGC CAACGTGGAG TTCATGGATG AGGCTCTGAT CATGGCAAGT ATGGATCACC 
     3361 CACACCTAGT TCGCCTATTG GGAGTGTGTC TGAGTCCCAC TATCCAGTTG GTTACGCAGC 
     3421 TGATGCCGCA TGGCTGCCTA CTGGACTATG TTCATGAACA CAAGGATAAC ATTGGATCAC 
     3481 AGCTGCTGTT GAACTGGTGT GTCCAGATTG CTAAGGGAAT GATGTACCTA GAAGAAAGAC 
     3541 GGCTTGTTCA TCGGGATCTG GCAGCCCGCA ATGTCTTAGT GAAATCTCCA AATCATGTTA 
     3601 AAATCACAGA TTTTGGACTG GCCCGGCTCT TGGAAGGAGA TGAAAAAGAA TACAATGCTG 
     3661 ATGGTGGCAA GATGCCAATT AAATGGATGG CTCTGGAATG TATACATTAT AGGAAATTCA 
     3721 CACATCAAAG TGATGTTTGG AGCTATGGCG TCACTATATG GGAACTGATG ACCTTTGGAG 
     3781 GAAAGCCCTA TGATGGAATT CCAACCCGAG AAATCCCCGA TTTACTGGAG AAAGGAGAGC 
     3841 GTCTGCCTCA GCCTCCCATC TGCACTATTG ATGTTTACAT GGTCATGGTC AAATGTTGGA 
     3901 TGATCGATGC TGACAGCAGA CCTAAATTCA AAGAACTGGC TGCTGAGTTT TCAAGAATGG 
     3961 CTAGAGACCC TCAAAGATAC CTAGTTATTC AGGGTGATGA TCGTATGAAG CTTCCCAGTC 
     4021 CAAATGACAG CAAATTCTTC CAGAATCTCT TGGATGAAGA GGATTTGGAA GACATGATGG 
     4081 ATGCTGAGGA ATATTTGGTC CCCCAGGCTT TCAACATACC TCCTCCCATC TACACATCCA 
     4141 GAACAAGAAT TGACTCCAAT AGGAATCAGT TTGTGTACCA AGATGGGGGC TTTGCTACAC 
     4201 AACAAGGAAT GCCCATGCCC TACAGAGCCA CAACCAGCAC CATACCAGAG GCTCCAGTAG 
     4261 CTCAGGGTGC AACGGCTGAG ATGTTTGATG ACTCCTGCTG TAATGGTACC CTACGAAAGC 
     4321 CAGTGGCACC CCATGTCCAA GAGGACAGTA GCACTCAGAG GTATAGTGCT GATCCCACAG 
     4381 TGTTCGCCCC AGAACGGAAT CCTCGAGGAG AACTGGATGA AGAAGGCTAC ATGACTCCAA 
     4441 TGCATGACAA GCCCAAACAA GAATATCTGA ATCCTGTGGA AGAGAACCCT TTTGTGTCCC 
     4501 GAAGGAAGAA TGGAGATCTT CAAGCTTTAG ATAATCCGGA GTATCACAGT GCTTCCAGCG 
     4561 GTCCACCCAA GGCGGAGGAT GAATACGTGA ATGAGCCTCT ATACCTCAAC ACCTTCGCCA 
     4621 ATGCCTTGGG GAGTGCAGAG TACATGAAAA ACAGTGTACT GTCTGTGCCA GAGAAAGCCA 
     4681 AGAAAGCATT TGACAACCCC GACTACTGGA ACCACAGCCT GCCACCCCGG AGCACCCTTC 
     4741 AGCACCCAGA CTACCTGCAG GAATACAGCA CAAAATATTT TTATAAACAG AATGGACGGA 
     4801 TCCGCCCCAT TGTGGCAGAG AATCCTGAGT ACCTCTCGGA GTTCTCGCTG AAGCCTGGCA 
     4861 CTATGCTGCC CCCTCCGCCC TACAGACACC GGAATACTGT GGTGACGCGT ACGCGGCCGC 
     4921 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4981 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5041 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5101 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5161 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5221 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5281 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5341 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5401 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5461 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5521 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5581 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5641 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5701 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5761 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5821 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5881 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5941 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6001 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6061 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6121 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6181 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6241 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6301 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6361 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6421 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6481 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6541 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6601 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6661 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6721 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6781 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6841 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6901 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6961 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7021 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7081 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7141 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7201 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7261 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7321 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7381 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7441 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7501 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7561 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7621 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7681 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7741 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7801 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7861 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7921 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7981 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8041 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8101 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8161 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8221 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8281 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8341 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8401 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8461 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8521 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8581 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8641 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8701 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//