LOCUS       MR225917                9501 bp    DNA     circular  SYN 
DEFINITION  Gli2 (Myc-DDK-tagged) - Mouse GLI-Kruppel family member GLI2 (Gli2)
ACCESSION   NM_001081125
VERSION     NM_001081125.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5658..5749 
                     /label="Myc-DDK"  
     misc_feature    5779..6364 
                     /label="PolyA signal"  
     misc_feature    5836..5855 
                     /label="Restriction Enzyme"  
     misc_feature    6640..7312 
                     /label="ColE1"  
     misc_feature    7742..8536 
                     /label="Kan/Neo"  
     misc_feature    8571..8873 
                     /label="SV40 ori"  
     misc_feature    8959..9436 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5640..5646 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5640 
                     /label="Gli2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGACTTCT GCCCCAGCCC CTGCACTGGA GAAGAAAGAA GCCAAGAGTG 
     1081 GTCTCTTGGA GGACAGCAGC TTCCCCGACC CAGGGAAAAA GGCCTGTCCT CTGGCGGTGG 
     1141 CCGCAGCTGT AGCCGCCCAC GGAGTTCCTC AGCAGCTCCT GCCGGCTTTC CACGCGCCTT 
     1201 TGCCGATTGA CATGAGACAC CAGGAGGGAA GGTACCATTA TGACCCTCAC TCTGTCCACA 
     1261 GTGTACACGG GCCTCCCACC CTAAGTGGCA GCCCCTTCAT CTCAGATATC TCCTTGATAC 
     1321 GACTTTCTCC ACACCCTGCT GGCCCTGGAG AGTCACCCTT CAGCGCCCAC CACCCCTACG 
     1381 TGAACCCCCA TATGGAGCAC TACCTCCGGT CTGTGCACAG CAGCCCCACA CTCTCAATGA 
     1441 TCTCTGCCGC CAGGGGCCTC AGCCCTGCTG ATGTGGCCCA CGAACATCTG AAAGAGAGGG 
     1501 GACTCTTTAG CCTCGCAGCC CCAGGCACCA ACCCTTCAGA CTATTACCAC CAGATGACCC 
     1561 TCATGGCAAG CCACCCCACC CCTTATGGGG ACCTTCTAAT GCAGAGCGGG GGTGCTGCTA 
     1621 GCGCACCCCA TCTCCATGAC TACCTCAACC CTGTGGATGC ATCACGATTC TCTAGTCCAC 
     1681 GTGTGACCCC ACGACTGAGC CGCAAGCGGG CTCTGTCCAT CTCCCCGCTC TCAGATGCCA 
     1741 GCCTCGACCT ACAACGCATG ATTCGGACCT CTCCCAACTC GCTGGTAGCT TACATCAACA 
     1801 ACTCCAGGAG CAGCTCAGCA GCCAGTGGCT CTTATGGACA TCTGTCTGCT GGTGCCCTCA 
     1861 GCCCAGCCTT CACTTTTCCC CACCCCATCA ATCCTGTGGC CTACCAGCAG ATCCTGAGCC 
     1921 AGCAGCGGGG CCTGGGCTCA GCCTTTGGAC ACACACCACC CCTGATCCAG CCTTCACCCA 
     1981 CCTTCTTGGC CCAGCAGCCC ATGACTCTCA CCTCCATCAG CACCATGCCT ACCCAACTCA 
     2041 GCAGCAGTAG CAGCAACTGT CTAAATGATG CCAACCAGAA CAAGCAGAAC AGCGAGTCAG 
     2101 CTGTGAGCAG CACCGTGAAC CCCATCACCA TTCATAAGCG GAGCAAGGTC AAGACTGAGG 
     2161 CTGAGGGCCT GCGTCCAGCA TCCCCGCTTG GACTGACACA GGAGCAGCTG GCCGATCTCA 
     2221 AGGAAGACCT GGACAGGGAT GACTGTAAGC AGGAGGCCGA GGGTGTCATC TACGAGACCA 
     2281 ACTGCCACTG GGCAGACTGC ACCAAGGAGT ATGACACACA GGAGCAGCTG GTGCATCATA 
     2341 TCAACAATGA ACACATCCAC GGGGAGAAGA AGGAGTTCGT GTGCCGCTGG CAGGCCTGCA 
     2401 CGAGAGAGCA GAAGCCCTTC AAGGCCCAGT ACATGCTGGT TGTTCACATG CGCAGACACA 
     2461 CGGGTGAGAA GCCACACAAG TGCACGTTCG AAGGCTGTTC CAAGGCCTAC TCTCGCCTGG 
     2521 AGAACCTGAA GACACACCTG CGTTCACACA CAGGAGAGAA GCCATATGTG TGTGAACACG 
     2581 AAGGCTGTAA CAAAGCCTTC TCCAATGCCT CAGACCGCGC CAAGCACCAG AACCGCACTC 
     2641 ACTCCAATGA GAAACCCTAC ATCTGCAAGA TCCCAGGCTG CACCAAGAGG TACACAGACC 
     2701 CCAGCTCACT CCGCAAGCAT GTGAAGACTG TCCATGGGCC AGACGCCCAT GTCACCAAGA 
     2761 AACAGCGTAA TGATGTGCAT GTCCGTGCTC CACTGCTCAA GGAGAATGGG GATAATGAGG 
     2821 CCAGCGCCGA GCCAGGTGGC CGGGGACCTG AGGAGAGTGT GGAGGCCAGT AGCACCAGCC 
     2881 ACACTGTGGA GGACTGCCTA CATATCAAAG CCATCAAGAC AGAGAGCTCC GGGCTTTGTC 
     2941 AGTCCAGCCC CGGGGCCCAG TCATCCTGCA GCAGCGAGCC CTCTCCCCTG GGCAGTGCCC 
     3001 CCAACAATGA CAGTGGCATG GAGATGCCGG GGACAGGGCC TGGGAGTCTG GGAGACCTGA 
     3061 CAGCACTGGC TGACACGTGT CCAGGAGCTG ACACCTCAGC CCTGGCTGCA CCCTCCACTG 
     3121 GTGGCCTGCA GCTGCGCAAA CACATGAGCA CCGTGCATCG CTTTGAGCAG CTGAAGAGAG 
     3181 AGAAGCTCAA GTCACTGAAG GATTCCTGCT CGTGGGCCGG CCCAGCTCCA CACACCCGCA 
     3241 ACACCAAGCT GCCTCCCCTT CCAGTCAATG GTTCTGTCCT GGAAAACTTC AACAATACAG 
     3301 GGGGCGGTGG ACCGGCAGGA CTGCTGCCCA GCCAGCGGCT ACCAGAGCTG ACCGAAGTGA 
     3361 CGATGCTGAG CCAGCTGCAG GAACGAAGAG ACAGCTCCAC CAGCACCATG AGCTCGGCCT 
     3421 ACACTGTGAG CCGCCGCTCC TCTGGCATCT CCCCATACTT CTCTAGCCGT CGCTCCAGCG 
     3481 AGGCTTCGCC TCTCGGTGGC CTACGCCCGC ACAACGCCAG CTCAGCAGAC TCCTATGACC 
     3541 CCATCTCCAC AGATGCCTCT CGGCGCTCCA GTGAAGCCAG CCAGTGCAGT GGCGGTGGCC 
     3601 CAGGGCTGCT CAACCTCACA CCTGCGCAGC AGTACAACCT GCGTGCCAAG TACGCAGCGG 
     3661 CCACAGGTGG ACCACCGCCC ACGCCACTGC CGGGCCTCGA TCGTGTAAGC CTTCGTACCC 
     3721 GCCTGGCCTT GCTGGATGCT CCTGAGCGTG CACTTCCTGG TGCCTGCCCA CATCCACTGG 
     3781 GGCCACGGCG TGGCAGCGAT GGGCCTACCT ATAGCCATGG TCATGGCCAT GGCTACGCAG 
     3841 GTGCGGCTCC AGCATTCCCC CACGAGGGGC CAAACAGCAG CACACGGCGG GCCAGCGACC 
     3901 CTGTGCGGCG CCCTGACCCC CTTATTCTGC CTCGAGTGCA ACGTTTCCAC AGTACCCACA 
     3961 ACATGAATCC AGGTTCACTG CCACCCTGCG CTGATCGGCG TGGCCTGCAC GTACAGAGCC 
     4021 ACCCCAGCGT AGACAGCAAC CTGACCCGCA ACGCCTACTC TCCCAGACCC CCTAGCATCA 
     4081 ATGAGAACGT GGTGATGGAG GCCGTGGCTG CTGGGGTAGA CGGCCCAGGG CTAGAGTGCG 
     4141 ACCTGGGGCT GGTGGAGGAT GAGCTGGTGC TGCCAGATGA TGTGGTACAG TACATCAAGG 
     4201 CTCACACCGG TGGTACCTTG GATGACGGCA TTCGGCAGGG GTATCCCACA GAAGGTACTG 
     4261 GCTTCCCCGA GAACTCTAAG CTGCCCAGTC CTGGGCTACA AGGCCACCGC AGGCTAGCAG 
     4321 CTGCCGACTC CAACATGGGT CCTTCTGCTC CTGGACTCGG GGGCTGCCAG CTGAGCTACA 
     4381 GCCCCTCCTC CAACCTCAAC AAGAGCAACA TGCCTGTGCA GTGGAATGAG GTGAGTTCTG 
     4441 GCACCGTGGA TGCCCTGCCT ACCCAGGTGA AGCCACCTCC TTTCCCTCAC AGCAACCTGG 
     4501 CTGTGGTCCA ACAGAAGCCA GCCTTTGGCC AGTATCCAGG ATATAATCCA CAATCCGTGC 
     4561 AGAGCAGCTC CGGAGGTCTA GACAGCACCC AGCCGCACCT ACAGCTTCGA GGAGCCCCCT 
     4621 CTGCATCAAG AGGGAGCTAC ACGCAACAGC CTCGACAGCC AGCTGCAGGC AGTCAGTGCC 
     4681 TGGGTATGAG TGCGGCCATG AGCCCGCAGG CCAGCTACAG CCAAGCCCAC CCCCAGCTGA 
     4741 GCCCAAACAT TGTCAGCGGA TCTCTGAACC AGTTTTCTCC CTCCTGCAGC AATATGGCAG 
     4801 CCAAGCCCAG CCACCTGGGA CTCCCTCAGC AAATGGAAGT TGTCCCCAAT GCCACCATCA 
     4861 TGAATGGCCA TCAACGGGAG CACGGGGTCC CCAATTCATC CCTGGCTGCG GTGTCACAAC 
     4921 CTCACCCAGT CCTGAGCTAT CCCCAGCAGG ACAGCTACCA ACAGGGCTCC AACCTTCTGT 
     4981 CATCCCATCA GCCTGGCTTC ATGGAGTCCC AGCAGAACGC GGGCTTTGGT CTCATGCAGC 
     5041 CTCGGCCACC CCTGGAACCC AACACGGCCA GCCGTCACCG TGGAGTACGT TCTGGGCAAC 
     5101 AGCAGTTGTA TGCCAGGACC ACTGGCCAAG CCATGGTCAC ATCAGCCAAC CAAGAGACAG 
     5161 CAGAAGCTAT GCCCAAGGGA CCAGCAGGGA CCATGGTATC CCTAGCTCCT CAGCCATCTC 
     5221 AGGACACAGG GCGGGCACAA GATCAGAACA CGCTATACTA CTATGGCCAG ATCCACATGT 
     5281 ATGAACAGAA TGGAGGCTGC CCAGCCGTGC AGCCCCAGCC GCCACAACCA CAAGCTTGCT 
     5341 CAGACAGTAT CCAGCCTGAG CCTTTGCCTT CACCGGGAGT CAACCAGGTG TCTAGCACCG 
     5401 TGGACTCCCA GCTCCTGGAG CCCCCCCAGA TTGACTTTGA TGCCATCATG GATGATGGTG 
     5461 ATCACTCGAG TTTGTTTTCT GGTGCACTGA GCCCAACCCT TCTCCACAAT CTCTCCCAGA 
     5521 ATTCCTCACG CCTCACCACA CCCCGGAATT CCTTGACACT GCCCTCCATC CCTGCGGGCA 
     5581 TCAGCAACAT GGCCGTGGGC GACATGAGTT CCATGCTCAC CAGCCTGGCT GAAGAGAGCA 
     5641 AGTTTTTTAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5701 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5761 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5821 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5881 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5941 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     6001 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     6061 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     6121 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     6181 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     6241 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     6301 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     6361 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     6421 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     6481 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     6541 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     6601 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6661 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6721 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6781 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6841 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6901 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6961 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     7021 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     7081 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     7141 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     7201 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     7261 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     7321 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     7381 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     7441 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     7501 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     7561 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     7621 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7681 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7741 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7801 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7861 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7921 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7981 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     8041 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     8101 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     8161 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     8221 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     8281 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     8341 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     8401 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     8461 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     8521 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     8581 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     8641 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8701 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8761 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8821 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8881 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8941 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     9001 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     9061 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     9121 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     9181 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     9241 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     9301 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     9361 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     9421 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     9481 CAAAAATTTA ACGCGAATTT T
//