LOCUS       MR225914                7497 bp    DNA     circular  SYN 
DEFINITION  Grm2 (Myc-DDK-tagged) - Mouse glutamate receptor, metabotropic 2 (Grm2)
ACCESSION   NM_001160353
VERSION     NM_001160353.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3654..3745 
                     /label="Myc-DDK"  
     misc_feature    3775..4360 
                     /label="PolyA signal"  
     misc_feature    3832..3851 
                     /label="Restriction Enzyme"  
     misc_feature    4636..5308 
                     /label="ColE1"  
     misc_feature    5738..6532 
                     /label="Kan/Neo"  
     misc_feature    6567..6869 
                     /label="SV40 ori"  
     misc_feature    6955..7432 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3636..3642 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3636 
                     /label="Grm2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAATCCCTG CTTAGGTTCC TGGCACTGCT GCTGCTGCGG GGTGCTGTGG 
     1081 CCGAGGGCCC GGCCAAGAAG GTGCTGACCC TGGAGGGGGA CCTGGTGCTG GGTGGGCTGT 
     1141 TCCCAGTGCA CCAGAAGGGT GGCCCAGCAG AGGAGTGTGG ACCTGTTAAT GAGCACCGTG 
     1201 GCATACAGCG CCTAGAGGCC ATGCTTTTTG CACTGGACCG CATCAACCGC GACCCCCATC 
     1261 TGCTGCCTGG GGTGCGTTTG GGCGCGCACA TCCTCGACAG CTGCTCCAAG GATACACACG 
     1321 CCCTGGAGCA GGCCCTGGAC TTTGTGCGTG CCTCACTCAG CCGTGGGGCT GATGGCTCAC 
     1381 GCCACATCTG TCCTGACGGC TCCTATGCTA CTCTCAGTGA TGCTCCCACA GCTATCACCG 
     1441 GTGTCATTGG CGGCTCCTAC AGTGATGTCT CCATCCAGGT GGCCAATCTC CTGCGGCTGT 
     1501 TCCAGATCCC ACAGATCAGC TATGCCTCCA CCAGCGCCAA GCTGAGCGAC AAGTCCCGTT 
     1561 ATGATTACTT TGCTCGCACC GTGCCCCCAG ACTTCTTCCA AGCCAAGGCC ATGGCTGAGA 
     1621 TTCTCCGCTT TTTCAACTGG ACGTATGTGT CTACGGTGGC ATCTGAGGGT GACTATGGAG 
     1681 AGACAGGCAT TGAGGCCTTC GAGCTCGAGG CTCGGGCACG CAACATCTGC GTGGCCACTT 
     1741 CGGAGAAGGT GGGCCGTGCC ATGAGCCGCG CAGCCTTCGA GGGTGTGGTG CGAGCCCTGT 
     1801 TGCAGAAGCC CAGCGCCCGT GTGGCTGTGC TCTTCACCCG GTCAGAGGAT GCCCGTGAGC 
     1861 TGCTTGCAGC CACCCAGCGT CTCAACGCCA GCTTCACCTG GGTAGCCAGC GACGGCTGGG 
     1921 GGGCCCTAGA GAGCGTGGTG GCAGGCAGTG AAAGGGCTGC CGAGGGCGCC ATCACCATTG 
     1981 AGCTGGCCTC CTACCCCATC AGTGACTTTG CCTCCTACTT CCAGAACTTG GATCCCTGGA 
     2041 ACAACAGCAG GAACCCTTGG TTCCGTGAGT TCTGGGAGGA GAGGTTCCGT TGCAGCTTCC 
     2101 GGCAGCGAGA CTGCGCCGCC CACTCTCTGC GGGCCGTACC CTTTGAACAG GAGTCAAAGA 
     2161 TCATGTTTGT GGTCAATGCC GTGTATGCCA TGGCCCACGC TCTGCACAAC ATGCACCGTG 
     2221 CCCTCTGTCC CAACACCACC CGCCTCTGCG ATGCCATGAG ACCTGTCAAT GGGCGCCGCC 
     2281 TCTACAAGGA CTTCGTGCTC AATGTCAAGT TTGATGCCCC CTTCCGCCCA GCAGATACCG 
     2341 ACGACGAGGT CCGCTTTGAC CGCTTTGGAG ATGGTATTGG CCGCTACAAC ATCTTCACCT 
     2401 ATCTGCGGGC AGGCAATGGG CGCTATCGCT ACCAGAAGGT AGGCTACTGG GCAGAAGGTC 
     2461 TGACTCTGGA TACTAGCATC ATCCCATGGG CCTCCCCATC AGCTGGCACC CTCCCTGCCT 
     2521 CTCGCTGTAG CGAGCCCTGC CTTCAGAATG AGGTGAAGAG CGTGCAGCCC GGCGAGGTCT 
     2581 GCTGCTGGCT CTGCATCCCC TGTCAGCCCT ATGAGTACAG GCTGGATGAG TTCACCTGTG 
     2641 CTGACTGTGG CCTGGGCTAC TGGCCTAATG CCAGTCTGAC TGGATGCTTT GAGCTGCCCC 
     2701 AGGAGTACAT CCGCTGGGGC GACGCCTGGG CGGTGGGACC TGTCACCATC GCCTGCCTAG 
     2761 GTGCCCTGGC CACCCTCTTT GTGTTGGGTG TCTTCGTGCG GCATAACGCC ACACCCGTGG 
     2821 TCAAGGCCTC GGGGCGGGAG CTTTGCTACA TTCTGCTGGG AGGCGTCTTC CTCTGCTACT 
     2881 GCATGACCTT CATCTTCATC GCCAAGCCCT CCACGGCCGT CTGCACCTTA AGGCGCCTCG 
     2941 GGTTGGGGAC CGCCTTCTCT GTCTGCTACT CAGCCCTCCT CACCAAGACC AATCGCATCG 
     3001 CTCGCATCTT TGGCGGGGCC CGGGAGGGTG CCCAGCGGCC GCGCTTCATC AGTCCCGCCT 
     3061 CACAGGTGGC CATCTGCCTG GCACTTATCT CGGGCCAGCT GCTCATTGTT GCTGCCTGGC 
     3121 TGGTGGTGGA GGCACCTGGC ATAGGCAAGG AGACAGCCCC TGAGCGGCGG GAAGTGGTGA 
     3181 CCTTGCGCTG TAACCACCGT GACGCAAGCA TGCTTGGCTC GCTGGCCTAC AACGTGCTCC 
     3241 TCATCGCTCT CTGCACGCTC TATGCCTTCA AGACCCGCAA GTGCCCGGAG AACTTCAACG 
     3301 AGGCCAAGTT CATCGGCTTT ACCATGTACA CCACCTGTAT CATCTGGCTG GCTTTCCTTC 
     3361 CCATCTTCTA CGTCACCTCC AGTGATTATC GGGTGCAGAC CACTACGATG TGCGTGTCCG 
     3421 TCAGCCTCAG TGGCTCTGTG GTGCTCGGCT GCCTCTTTGC ACCCAAGCTG CACATTATCC 
     3481 TCTTCCAGCC ACAGAAGAAC GTGGTGAGCC ACCGGGCACC TACCAGCCGC TTTGGCAGTG 
     3541 CTGCCCCCAG GGCCAGCGCC AACCTTGGTC AAGGGTCTGG ATCCCAGCTT GTTCCTACTG 
     3601 TTTGCAATGG CCGTGAGGTG GTAGACTCAA CAACATCGTC GCTTACGCGT ACGCGGCCGC 
     3661 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3721 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3781 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3841 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3901 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3961 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4021 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4081 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4141 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4201 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4261 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4321 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4381 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4441 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4501 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4561 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4621 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4681 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4741 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4801 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4861 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4921 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4981 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5041 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5101 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5161 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5221 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5281 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5341 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5401 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5461 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5521 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5581 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5641 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5701 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5761 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5821 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5881 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5941 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6001 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6061 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6121 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6181 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6241 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6301 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6361 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6421 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6481 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6541 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6601 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6661 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6721 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6781 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6841 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6901 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6961 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7021 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7081 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7141 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7201 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7261 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7321 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7381 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7441 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//