LOCUS       MR225912                6996 bp    DNA     circular  SYN 
DEFINITION  Fbln1 (Myc-DDK-tagged) - Mouse fibulin 1 (Fbln1)
ACCESSION   NM_010180
VERSION     NM_010180.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3153..3244 
                     /label="Myc-DDK"  
     misc_feature    3274..3859 
                     /label="PolyA signal"  
     misc_feature    3331..3350 
                     /label="Restriction Enzyme"  
     misc_feature    4135..4807 
                     /label="ColE1"  
     misc_feature    5237..6031 
                     /label="Kan/Neo"  
     misc_feature    6066..6368 
                     /label="SV40 ori"  
     misc_feature    6454..6931 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3135..3141 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3135 
                     /label="Fbln1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCGCCCC GTGCCGTCGC GCCTCGTCCC GCTGCCGCTG CTGCTGCTCA 
     1081 GCAGCCTCTC GCTGCTGGCA GCCCGAGCGA ATGCAGACAT CTCCATGGAG GCTTGCTGCA 
     1141 CGGATGGAAA TCAGATGGCT AACCAGCACA GGGACTGCTC GCTGCCGTAC ACCTCAGAAT 
     1201 CCAAGGAGTG CAGGATGGTC CAGGAGCAAT GTTGTCACAA CCAACTGGAA GAGCTGCACT 
     1261 GTGCCACGGG CATCAACCTG GCCAGCGAGC CAGAAGGCTG CGCCTCGCTC CACAGCTACA 
     1321 ACAGTAGCCT TGAGACCATC TTCATAAAGA GGTGCTGCCA CTGTTGCATG CTGGGAAAGG 
     1381 CATCCCTGGC CCGAGACCAG ACCTGTGAAC CCATTGTCAT GATAAGCTAC CAGTGTGGGC 
     1441 TGGTGTTCCG TGCCTGCTGT GTGAAGGCCC GGGAGAATTC AGACTTTGTC CAAGGCAACG 
     1501 GTGCAGACCT TCAGGACCCA GCTAAGATTC CTGACGAGGA GGACCAAGAA GACCCGTACC 
     1561 TGAATGACCG CTGTCGAGGT GGCGGGCCCT GTAAGCAGCA GTGCCGTGAC ACTGGGGACG 
     1621 AGGTGATCTG CTCTTGCTTT GTGGGCTACC AGCTGCAGTC GGATGGTGTC TCCTGCGAAG 
     1681 ATATCAATGA ATGCATCACA GGCAGCCATA ACTGCCGGCT GGGAGAATCC TGCATCAATA 
     1741 CAGTGGGCTC TTTCCGCTGC CAGCGGGACA GCAGCTGTGG GACTGGCTAT GAGCTCACAG 
     1801 AGGATAATAA CTGCAAAGAT ATTGACGAAT GTGAGACTGG TATTCATAAC TGCCCCCCCG 
     1861 ATTTTATCTG TCAGAATACT CTGGGATCCT TCCGTTGCAG ACCGAAGCTG CAGTGCAAGA 
     1921 GCGGCTTCAT ACAGGATGCT CTAGGCAACT GCATTGATAT CAATGAGTGT TTAAGTATCA 
     1981 GTGCTCCATG CCCTGTGGGG CAGACATGCA TCAATACAGA GGGCTCCTAC ACATGCCAGA 
     2041 AGAATGTGCC CAACTGTGGC CGTGGTTATC ATCTCAATGA AGAGGGGACC CGCTGTGTTG 
     2101 ATGTGGATGA GTGCTCCCCA CCAGCGGAGC CCTGTGGGAA GGGACACCAC TGCCTGAACT 
     2161 CCCCCGGCAG CTTCCGCTGC GAGTGCAAGG CTGGCTTCTA TTTTGATGGC ATCAGCAGGA 
     2221 CCTGCGTGGA TATCAACGAG TGCCAGCGCT ATCCCGGGCG CCTGTGTGGC CACAAGTGTG 
     2281 AGAACACGCC AGGCTCCTTC CACTGCAGCT GCTCCGCCGG CTTCCGGCTG TCTGTGGACG 
     2341 GCCGGTCTTG TGAAGATGTG AACGAGTGTC TCAACAGCCC TTGCAGCCAG GAGTGTGCTA 
     2401 ATGTCTATGG CTCCTACCAG TGCTATTGCC GACGAGGTTA CCAGCTCAGT GACGTGGATG 
     2461 GGGTCACCTG CGAAGATATT GATGAGTGTG CCCTGCCCAC TGGAGGTCAC ATCTGCTCCT 
     2521 ACCGCTGCAT CAACATCCCC GGAAGCTTCC AGTGCAGCTG CCCCTCATCT GGCTACAGGC 
     2581 TAGCTCCCAA TGGCCGCAAC TGCCAAGACA TTGATGAGTG TGTGACCGGC ATCCATAACT 
     2641 GTTCCATCAA TGAGACTTGC TTCAACATCC AGGGCAGCTT CCGCTGTCTG TCCTTTGAAT 
     2701 GCCCCGAGAA CTATCGCCGC TCCGCAGACA CCTTCCGCCA AGAGAAAACA GACACCGTCC 
     2761 GCTGCATCAA GTCTTGCCGT CCCAACGATG AGGCCTGCGT GCGGGACCCT GTACATACCG 
     2821 TCTCCCACAC CGTCATCTCG CTGCCCACCT TTCGAGAGTT CACCCGTCCT GAGGAGATCA 
     2881 TCTTTCTGAG GGCTGTCACA CCGCTGTACC CCGCCAACCA GGCCGACATC ATCTTCGACA 
     2941 TCACAGAAGG GAACCTTCGA GACTCCTTTG ATATCATCAA GCGCTATGAG GACGGCATGA 
     3001 CTGTGGGTGT CGTGCGCCAA GTGCGGCCCA TCGTGGGCCC GTTTTACGCT GTCCTGAAGC 
     3061 TGGAGATGAA CTACGTGTTG GGAGGCGTAG TTTCCCACCG GAACGTCGTC AATGTACACA 
     3121 TCTTCGTCTC CGAGTACTGG TTCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3181 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3241 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3301 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3361 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3421 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3481 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3541 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3601 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3661 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     3721 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     3781 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     3841 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     3901 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     3961 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4021 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4081 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4141 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4201 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4261 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4321 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4381 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4441 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4501 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4561 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4621 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4681 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     4741 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     4801 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     4861 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     4921 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     4981 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5041 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5101 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5161 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5221 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5281 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5341 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5401 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5461 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5521 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5581 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5641 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     5701 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     5761 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     5821 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     5881 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     5941 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6001 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6061 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6121 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6181 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6241 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6301 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6361 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6421 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6481 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6541 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6601 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6661 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     6721 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     6781 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     6841 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     6901 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     6961 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//