LOCUS       MR225877                7608 bp    DNA     circular  SYN 
DEFINITION  Hace1 (Myc-DDK-tagged) - Mouse HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1)
ACCESSION   NM_172473
VERSION     NM_172473.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3765..3856 
                     /label="Myc-DDK"  
     misc_feature    3886..4471 
                     /label="PolyA signal"  
     misc_feature    3943..3962 
                     /label="Restriction Enzyme"  
     misc_feature    4747..5419 
                     /label="ColE1"  
     misc_feature    5849..6643 
                     /label="Kan/Neo"  
     misc_feature    6678..6980 
                     /label="SV40 ori"  
     misc_feature    7066..7543 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3747..3753 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3747 
                     /label="Hace1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAGAGCG ATGGAGCAGC TCAACCGTCT GACGCGCTCG CTGCGCCGCG 
     1081 CGCGCACCGT GGAGCTGCCC GAGGATAATG AGACTGCTGT TTACACACTG ATGCCAATGG 
     1141 TGATGGCCGA CCAGCACAGG TCTGTTTCTG AACTCCTATC AAATTCAAAA TTTGATGTCA 
     1201 ATTATGCATT CGGACGTGTG AAGAGAAGCT TGCTTCACAT TGCTGCAAAC TGTGGATCTG 
     1261 TGGAATGCTT GGTTCTTCTG TTGAAGAAAG GCGCAAACCC TAACTATCAA GATATTTCAG 
     1321 GCTGTACACC CCTTCATCTC GCTGCTAGAA ATGGACAGAA GAAATGCATG AGTAAATTAC 
     1381 TGGAGTACAG TGCGGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA ATACACTGGC 
     1441 TGGCTGTGAA TGGGAGGACA GAGCTCCTCC ATGACCTCGT GCAACACGTC ACAGATGTGG 
     1501 ATGTGGAGGA TGCCATGGGG CAGACAGCGC TACACGTAGC CTGCCAGAAC GGGCACAAAA 
     1561 CGACAGTGCA GTGCTTGCTA GACAGCGGTG CTGATATTAA CAGGCCGAAT GTATCAGGAG 
     1621 CCACGCCACT GTACTTTGCT TGCAGTCATG GTCAGAGAGA TACTGCACAG ATTCTTCTAT 
     1681 TACGAGGAGC CAAATATTTA CCAGATAAAA ATGGAGTAAC CCCTCTAGAT CTATGTGTAC 
     1741 AGGGTGGTTA CGGACAGACT TGTGAAGTGT TAATTCAGTA CCACCCGAGG CTTTTCCAGA 
     1801 CTATTGTTCA AATGACACAG AATGAAGACC TTCGGGAAAA CATGTTACGG CAAGTTCTGC 
     1861 AGCATTTGTC TCAGCAAAGT GAAAGCCAGT ACCTAAAGAT CCTGACAGGC CTTGCTGAAG 
     1921 TTGCTACGAC AAATGGCCAT AAGCTGCTTA GTTTATCTAG CAATTATGAT GCGCAGATGA 
     1981 AGAGCCTTTT GAGGATTGTG AGGATATTTT GTCATGTCTT TCGAATTGGT CCCTCATCTC 
     2041 CCAGTAATGG AATCGATATG GGCTACAATG GAAATAAAAC TCCAAGAAGC CAGGTGTTCA 
     2101 AGCCTTTGGA ATTGCTTTGG CACTCATTGG ATGAATGGTT AGTTTTAATA GCCACAGAAT 
     2161 TAATGAAAAA CAAAGAGGAT TCAACAGATA TTACTTCTAT CTTGCTGAAA CAAAAAGGCC 
     2221 AGGATCAAGA GGCCCCTTCT CTTTCTGCAT TTGAGCCACC AGGCCCTGGG AGCTATGAAA 
     2281 GCCTGCCCCC TGGTCCAGGG GACTCCAAGC CAGAGGTGCT TGCAGGGGAG CAGGAAGCCA 
     2341 GTGCCGACTG TCAAGACGTC ATCTCTGTGA CAGCCAACCG GCTGAGTGCT GTCATTCAAG 
     2401 CCTTCTACAT GTGCTGTTCC TGTCAGATGC CTCCAGGAAT GACTTCACCT CGTTTCATTG 
     2461 AGTTTGTCTG CAAGCATGAT GAAGTATTGA AATGCTTTGT GAATAGAAAC CCCAAAATTA 
     2521 TATTTGACCA TTTTCACTTT CTTCTTGAAT GTCCTGAATT GATGTCAAGA TTCATGCATA 
     2581 TCATAAAAGC ACAGCCATTT AAAGATCGAT GTGAGTGGTT CTATGAACAT CTGCACTCAG 
     2641 GACAGCCGGA CTCAGACATG GTCCACAGAC CAGTGAGTGA GAACGATATC CTCCTGGTTC 
     2701 ACAGAGATTC TATTTTTAGG AGTAGCTGTG AAATTGTATC AAAAGCAAAC TGTGCAAAGC 
     2761 TAAAGCAGGG GATTGCTGTA CGATTTCATG GAGAAGAAGG CATGGGTCAA GGCGTTGTGC 
     2821 GTGAATGGTT TGATATTCTG TCTAATGAGA TCGTCAACCC TGACTATGCA CTGTTCACCC 
     2881 AGTCAGCAGA CGGAACAACT TTCCAGCCCA ACAGCAACTC CTATGTCAAT CCTGATCACT 
     2941 TGAACTATTT CCGGTTTGCT GGACAGATCT TGGGGTTAGC TTTGAACCAC AGGCAGCTGG 
     3001 TCAATATTTA CTTCACACGA TCATTCTACA AGCACATTCT CGGTATTCCT GTAAATTACC 
     3061 AAGATGTAGC ATCTATTGAT CCTGAATATG CCAAAAATTT GCAATGGATT TTAGATAATG 
     3121 ATATTAGTGA CTTGGGTCTA GAATTGACTT TTTCTGTTGA GACTGATGTA TTTGGAGCTA 
     3181 TGGAAGAGGT GCCTTTAAAG CCTGGGGGTG GAAGTATTCT TGTGACACAA AATAACAAAG 
     3241 CGGAGTATGT CCAGCTCGTT ACTGAACTGC GTATGACAAG AGCCATTCAG CCGCAGATCA 
     3301 ATGCGTTTTT ACAGGGTTTT CATATGTTCA TCCCGCCTTC CCTCATACAA CTGTTTGACG 
     3361 AGTATGAATT GGAGCTACTG CTTTCTGGCA TGCCGGAAAT TGATGTAAAT GATTGGATAA 
     3421 AAAATACAGA ATACACAAGT GGCTATGAAA GAGAAGATCC AGTTATTCAG TGGTTCTGGG 
     3481 AAGTTGTGGA AGACATTACT CAAGAAGAAA GAGTTCTTCT CTTGCAGTTC GTTACTGGCA 
     3541 GTTCCAGGGT CCCGCATGGT GGCTTTGCCA ATATTATGGG TGGAAGTGGT TTGCAAAACT 
     3601 TTACAATTGC TGCTGTGCCA TATACTCCAA ATCTTTTACC AACTTCCAGC ACATGCATCA 
     3661 ACATGCTCAA GTTACCTGAA TACCCAAGTA AAGAAATACT CAAGGACAGA CTCCTTGTAG 
     3721 CACTGCATTG TGGCAGCTAT GGTTATACAA TGGCAACGCG TACGCGGCCG CTCGAGCAGA 
     3781 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3841 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3901 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3961 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4021 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4081 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4141 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4201 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4261 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4321 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4381 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4441 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4501 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4561 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4621 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4681 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4741 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4801 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4861 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4921 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4981 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5041 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5101 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5161 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5221 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5281 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5341 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5401 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5461 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5521 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5581 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5641 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5701 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5761 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5821 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5881 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5941 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6001 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6061 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6121 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6181 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6241 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6301 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6361 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6421 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6481 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6541 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6601 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6661 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6721 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6781 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6841 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6901 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6961 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7021 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7081 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7141 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7201 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7261 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7321 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7381 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7441 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7501 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7561 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//