LOCUS       MR225857                8916 bp    DNA     circular  SYN 
DEFINITION  Pnpla6 (Myc-DDK-tagged) - Mouse patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 1
ACCESSION   NM_001122818
VERSION     NM_001122818.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5073..5164 
                     /label="Myc-DDK"  
     misc_feature    5194..5779 
                     /label="PolyA signal"  
     misc_feature    5251..5270 
                     /label="Restriction Enzyme"  
     misc_feature    6055..6727 
                     /label="ColE1"  
     misc_feature    7157..7951 
                     /label="Kan/Neo"  
     misc_feature    7986..8288 
                     /label="SV40 ori"  
     misc_feature    8374..8851 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5055..5061 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5055 
                     /label="Pnpla6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGAGTCA CTCCAGCCAA AGGACTGCAG GCTACAAGAC CTGCTACCTA 
     1081 GTCCGAAACA ATCGATGGAG GCCCCACTGC AAACCGGAAT GGTGCTGGGC GTGATGATCG 
     1141 GGGCCGGAGT GGCGGTGCTG GTCACCGCCG TGCTCATCCT CTTGGTGGTG CGAAGGCTGC 
     1201 GGGTGCAGAA AACTCCAGCC CCGGAGGGTC CCCGGTATCG GTTCCGGAAG CGGGACAAAG 
     1261 TGCTTTTCTA TGGCCGGAAG ATTATGCGGA AGGTGTCACA GTCCACTTCT TCCCTGGTGG 
     1321 ACACATCAGT CTCCACCACT TCCCGGCCCC GCATGAAGAA GAAACTGAAG ATGCTTAACA 
     1381 TTGCCAAGAA GATCTTACGT ATCCAAAAGG AGACTCCGAC CCTACAACGG AAGGAGCCCC 
     1441 CACCTTCAGT GCTGGAGGCT GACCTCACAG AGGGAGACCT GGCCAATTCC CACCTACCCT 
     1501 CTGAGGTACT CTACATGCTT AAGAATGTTC GGGTGCTAGG TCACTTTGAG AAGCCGCTCT 
     1561 TCCTGGAGCT CTGCCGGCAC ATGGTCTTCC AGCGTCTCGG GCAGGGGGAC TATGTTTTTC 
     1621 GGCCCGGTCA ACCAGATGCC AGCATCTATG TGGTTCAAGA TGGGCTGCTG GAGCTCTGCC 
     1681 TACCAGGGCC TGATGGGAAG GAGTGTGTGG TGAAGGAGGT GGTTCCTGGA GACAGCGTCA 
     1741 ACAGCCTTCT GAGCATCCTT GATGTCATCA CGGGTCACCA GCATCCCCAG CGGACTGTGT 
     1801 CAGCCAGGGC TGCACGGGAC TCCACAGTGC TGAGACTCCC AGTAGAAGCC TTCTCTGCTG 
     1861 TCTTCACCAA GTACCCTGAG AGTTTGGTGC GAGTGGTACA GATCATCATG GTAAGGCTGC 
     1921 AGAGAGTCAC CTTCCTGGCG CTTCACAATT ACCTGGGTCT GACCAATGAA CTCTTCAGTC 
     1981 ATGAGATCCA GCCCCTACGC CTTTTCCCCA GCCCCGGCCT CCCGACCCGA ACCAGCCCTG 
     2041 TCCGTGGCTC TAAACGGGTA GTCAGCACCT CAGGTACTGA GGACACCTCA AAGGAGACCT 
     2101 CTGGCCGGCC CCTGGACTCC ATTGGAGCTC CTCTGCCTGG ACCTGCAGGG GATCCCGTGA 
     2161 AGCCCACATC CTTAGAAGCA CCCCCAGCCC CGTTACTGAG CCGCTGCATC TCCATGCCAG 
     2221 TAGACATCTC AGGCTTGCAA GGTGGCCCTC GTTCTGATTT TGACATGGCC TATGAACGTG 
     2281 GACGGATATC TGTTTCTCTT CAAGAAGAGG CATCTGGGGG ACCTCAGACA GCATCCCCTA 
     2341 GGGAGCTCCG GGAGCAGCCA GCGGGTGCCT GTGAATATAG CTACTGTGAG GATGAGTCAG 
     2401 CCACAGGCGG ATGTCCCTTT GGGCCCTACC AGGGCCGCCA GACAAGCAGC ATCTTTGAGG 
     2461 CTGCAAAGAG AGAGCTAGCC AAGCTGATGC GGATTGAGGA CCCCTCTCTA CTGAACAGCA 
     2521 GAGTCTTGCT ACATCATGCC AAAGCCGGCA CCATCATAGC CCGCCAAGGG GACCAGGATG 
     2581 TGAGCCTGCA CTTTGTGCTC TGGGGCTGCC TGCACGTGTA CCAGCGCATG ATTGACAAGG 
     2641 CTGAGGAAGT GTGCCTATTT GTGGCACAGC CAGGGGAGCT GGTGGGGCAA CTGGCGGTGC 
     2701 TCACTGGGGA ACCTCTCATC TTCACACTAC GTGCCCAGAG AGACTGCACC TTCCTGCGGA 
     2761 TCTCCAAATC CCACTTCTAT GAGATCATGC GTGCACAACC CAGTGTGGTA CTGAGTGCAG 
     2821 CTCACACGGT GGCTGCTAGA ATGTCCCCCT TCGTGCGCCA GATGGACTTT GCCATTGACT 
     2881 GGACAGCTGT GGAGGCTGGT CGTGCGCTCT ACAGGCAGGG AGACCGCTCC GACTGCACCT 
     2941 ACATTGTACT CAATGGACGT CTCCGTAGTG TCATCCAACG AGGCAGTGGC AAGAAGGAGC 
     3001 TAGTTGGGGA GTATGGTCGA GGGGATCTCA TTGGCGTGGT GGAGGCACTG ACCCGGCAGC 
     3061 CACGAGCCAC CACTGTACAC GCAGTGCGAG ACACTGAGCT GGCCAAGCTT CCCGAGGGCA 
     3121 CCTTAGGCCA CATCAAACGT CGGTACCCAC AGGTTGTGAC CCGCCTTATT CATCTGCTAA 
     3181 GCCAGAAAAT TCTAGGCAAT CTGCAGCAGT TGCAAGGACC CTTTCCAGGC TCCGGGCTCA 
     3241 GTGTTCCGCA GCACTCGGAA CTGACCAACC CAGCCAGCAA TCTGTCTACT GTAGCTATCC 
     3301 TCCCTGTGTG TGCTGAGGTG CCCATGATGG CCTTTACTCT GGAACTGCAG CATGCTCTGC 
     3361 AAGCTATTGG TCCCACGCTC CTCCTTAACA GTGACGTCAT CCGGGCACTC CTGGGGGCTT 
     3421 CAGCACTGGA TAGCATTCAA GAATTCCGGC TGTCAGGGTG GTTGGCCCAG CAGGAAGATG 
     3481 CCCATCGCAT TGTGCTCTAC CAAACTGACA CATCCCTGAC GCCTTGGACC GTCCGTTGTC 
     3541 TGCGCCAGGC CGACTGCATC CTCATTGTGG GCCTGGGGGA CCAGGAACCC ACTGTTGGCC 
     3601 AGCTGGAGCA AATGTTAGAG AACACTGCTG TCCGTGCCTT GAAGCAACTG GTTTTGCTGC 
     3661 ACCGGGAGGA AGGCCCTGGT CCCACGCGCA CTGTAGAGTG GCTCAACATG CGCAGCTGGT 
     3721 GCTCAGGGCA CCTGCACCTG CGCTGTCCTC GCCGCCTCTT CTCACGGCGC AGCCCAGCGA 
     3781 AACTTCATGA GCTGTATGAG AAGGTTTTCT CCAGGCGTGC AGACCGTCAC AGCGACTTCT 
     3841 CCCGCTTGGC ACGAGTACTC ACAGGAAACA CTATTGCTCT GGTGCTGGGT GGGGGCGGAG 
     3901 CCAGAGGCTG CTCGCATATT GGGGTGCTGA AGGCATTAGA GGAGGCAGGA GTCCCAGTCG 
     3961 ACCTTGTGGG AGGCACATCC ATAGGTTCCT TCATTGGGGC CTTGTATGCC GAGGAACGCA 
     4021 GCGCCAGCCG AACTAAACAA CGAGCCCGGG AGTGGGCCAA GAGCATGACT TCTGTACTGG 
     4081 AGCCTGTATT GGACCTCACA TATCCTGTCA CCTCCATGTT TACTGGCTCA GCCTTTAACA 
     4141 GAAGTATCCA CCGTGTCTTC CAGGATAAGC AGATTGAGGA CCTGTGGCTG CCTTACTTCA 
     4201 ATGTGACCAC AGACATCACT GCCTCTGCCA TGCGCGTCCA CAAAGATGGC TCCCTGTGGC 
     4261 GGTATGTACG TGCCAGCATG ACGCTCTCGG GCTACCTACC CCCGCTGTGC GACCCAAAGG 
     4321 ATGGGCACCT GCTCATGGAT GGTGGCTACA TCAACAACCT GCCAGCGGAT ATTGCCCGAA 
     4381 GCATGGGAGC CAAAACGGTC ATTGCCATCG ACGTTGGAAG CCAGGATGAG ACAGATCTCA 
     4441 GCACCTATGG GGACAGCCTG TCTGGCTGGT GGTTGCTGTG GAAAAGGCTA AACCCCTGGG 
     4501 CAGACAAGGT AAAGGTTCCA GACATGGCTG AGATCCAGTC CCGCTTGGCG TACGTGTCCT 
     4561 GTGTGCGGCA GCTAGAAGTC GTCAAGTCCA GCTCCTATTG CGAGTACCTT CGTCCATCCA 
     4621 TTGACTGCTT CAAGACCATG GACTTTGGAA AGTTTGACCA GATCTATGAT GTGGGTTACC 
     4681 AGTATGGGAA GGCTGTCTTT GGAGGCTGGA CCCGTGGTGA AGTCATTGAG AAAATGCTCA 
     4741 CAGACCGGAG ATCTACAGAC CTTAATGAGA GTCGCCGTGC AGATATACTT GCCTTCCCAA 
     4801 GTTCTGGGTT CACTGACTTG GCTGAGATTG TGTCCCGGAT CGAGCCACCA ACAAGCTACG 
     4861 TCTCTGATGG CTGTGCTGAT GGGGAGGAGT CGGATTGCTT GACAGAGTAT GAGGAAGATG 
     4921 CAGGACCAGA CTGCTCAAGA GATGAAGGGG GCTCCCCTGA GGGTGCCAGC CCTAGCACTG 
     4981 CCTCAGAGGT GGAAGAAGAG AAGTCCACAC TCCGGCAACG ACGCTTTCTG CCTCAGGAGA 
     5041 CTCCCAGCTC AGTCGCAGAT GCCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5101 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5161 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5221 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5281 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5341 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5401 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5461 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5521 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5581 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5641 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5701 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5761 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5821 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5881 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5941 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6001 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6061 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6121 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6181 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6241 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6301 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6361 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6421 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6481 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6541 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6601 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6661 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6721 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6781 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6841 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6901 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6961 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7021 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7081 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7141 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7201 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7261 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7321 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7381 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7441 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7501 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7561 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7621 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7681 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7741 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7801 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7861 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7921 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7981 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8041 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8101 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8161 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8221 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8281 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8341 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8401 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8461 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8521 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8581 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8641 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8701 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8761 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8821 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8881 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//