LOCUS       MR225856                8862 bp    DNA     circular  SYN 
DEFINITION  Pnpla6 (Myc-DDK-tagged) - Mouse patatin-like phospholipase domain containing 6 (Pnpla6), transcript variant 2
ACCESSION   NM_015801
VERSION     NM_015801.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5019..5110 
                     /label="Myc-DDK"  
     misc_feature    5140..5725 
                     /label="PolyA signal"  
     misc_feature    5197..5216 
                     /label="Restriction Enzyme"  
     misc_feature    6001..6673 
                     /label="ColE1"  
     misc_feature    7103..7897 
                     /label="Kan/Neo"  
     misc_feature    7932..8234 
                     /label="SV40 ori"  
     misc_feature    8320..8797 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5001..5007 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5001 
                     /label="Pnpla6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCCCCA CTGCAAACCG GAATGGTGCT GGGCGTGATG ATCGGGGCCG 
     1081 GAGTGGCGGT GCTGGTCACC GCCGTGCTCA TCCTCTTGGT GGTGCGAAGG CTGCGGGTGC 
     1141 AGAAAACTCC AGCCCCGGAG GGTCCCCGGT ATCGGTTCCG GAAGCGGGAC AAAGTGCTTT 
     1201 TCTATGGCCG GAAGATTATG CGGAAGGTGT CACAGTCCAC TTCTTCCCTG GTGGACACAT 
     1261 CAGTCTCCAC CACTTCCCGG CCCCGCATGA AGAAGAAACT GAAGATGCTT AACATTGCCA 
     1321 AGAAGATCTT ACGTATCCAA AAGGAGACTC CGACCCTACA ACGGAAGGAG CCCCCACCTT 
     1381 CAGTGCTGGA GGCTGACCTC ACAGAGGGAG ACCTGGCCAA TTCCCACCTA CCCTCTGAGG 
     1441 TACTCTACAT GCTTAAGAAT GTTCGGGTGC TAGGTCACTT TGAGAAGCCG CTCTTCCTGG 
     1501 AGCTCTGCCG GCACATGGTC TTCCAGCGTC TCGGGCAGGG GGACTATGTT TTTCGGCCCG 
     1561 GTCAACCAGA TGCCAGCATC TATGTGGTTC AAGATGGGCT GCTGGAGCTC TGCCTACCAG 
     1621 GGCCTGATGG GAAGGAGTGT GTGGTGAAGG AGGTGGTTCC TGGAGACAGC GTCAACAGCC 
     1681 TTCTGAGCAT CCTTGATGTC ATCACGGGTC ACCAGCATCC CCAGCGGACT GTGTCAGCCA 
     1741 GGGCTGCACG GGACTCCACA GTGCTGAGAC TCCCAGTAGA AGCCTTCTCT GCTGTCTTCA 
     1801 CCAAGTACCC TGAGAGTTTG GTGCGAGTGG TACAGATCAT CATGGTAAGG CTGCAGAGAG 
     1861 TCACCTTCCT GGCGCTTCAC AATTACCTGG GTCTGACCAA TGAACTCTTC AGTCATGAGA 
     1921 TCCAGCCCCT ACGCCTTTTC CCCAGCCCCG GCCTCCCGAC CCGAACCAGC CCTGTCCGTG 
     1981 GCTCTAAACG GGTAGTCAGC ACCTCAGGTA CTGAGGACAC CTCAAAGGAG ACCTCTGGCC 
     2041 GGCCCCTGGA CTCCATTGGA GCTCCTCTGC CTGGACCTGC AGGGGATCCC GTGAAGCCCA 
     2101 CATCCTTAGA AGCACCCCCA GCCCCGTTAC TGAGCCGCTG CATCTCCATG CCAGTAGACA 
     2161 TCTCAGGCTT GCAAGGTGGC CCTCGTTCTG ATTTTGACAT GGCCTATGAA CGTGGACGGA 
     2221 TATCTGTTTC TCTTCAAGAA GAGGCATCTG GGGGACCTCA GACAGCATCC CCTAGGACCC 
     2281 CCACTCAGGA GCTCCGGGAG CAGCCAGCGG GTGCCTGTGA ATATAGCTAC TGTGAGGATG 
     2341 AGTCAGCCAC AGGCGGATGT CCCTTTGGGC CCTACCAGGG CCGCCAGACA AGCAGCATCT 
     2401 TTGAGGCTGC AAAGAGAGAG CTAGCCAAGC TGATGCGGAT TGAGGACCCC TCTCTACTGA 
     2461 ACAGCAGAGT CTTGCTACAT CATGCCAAAG CCGGCACCAT CATAGCCCGC CAAGGGGACC 
     2521 AGGATGTGAG CCTGCACTTT GTGCTCTGGG GCTGCCTGCA CGTGTACCAG CGCATGATTG 
     2581 ACAAGGCTGA GGAAGTGTGC CTATTTGTGG CACAGCCAGG GGAGCTGGTG GGGCAACTGG 
     2641 CGGTGCTCAC TGGGGAACCT CTCATCTTCA CACTACGTGC CCAGAGAGAC TGCACCTTCC 
     2701 TGCGGATCTC CAAATCCCAC TTCTATGAGA TCATGCGTGC ACAACCCAGT GTGGTACTGA 
     2761 GTGCAGCTCA CACGGTGGCT GCTAGAATGT CCCCCTTCGT GCGCCAGATG GACTTTGCCA 
     2821 TTGACTGGAC AGCTGTGGAG GCTGGTCGTG CGCTCTACAG GCAGGGAGAC CGCTCCGACT 
     2881 GCACCTACAT TGTACTCAAT GGACGTCTCC GTAGTGTCAT CCAACGAGGC AGTGGCAAGA 
     2941 AGGAGCTAGT TGGGGAGTAT GGTCGAGGGG ATCTCATTGG CGTGGTGGAG GCACTGACCC 
     3001 GGCAGCCACG AGCCACCACT GTACACGCAG TGCGAGACAC TGAGCTGGCC AAGCTTCCCG 
     3061 AGGGCACCTT AGGCCACATC AAACGTCGGT ACCCACAGGT TGTGACCCGC CTTATTCATC 
     3121 TGCTAAGCCA GAAAATTCTA GGCAATCTGC AGCAGTTGCA AGGACCCTTT CCAGGCTCCG 
     3181 GGCTCAGTGT TCCGCAGCAC TCGGAACTGA CCAACCCAGC CAGCAATCTG TCTACTGTAG 
     3241 CTATCCTCCC TGTGTGTGCT GAGGTGCCCA TGATGGCCTT TACTCTGGAA CTGCAGCATG 
     3301 CTCTGCAAGC TATTGGTCCC ACGCTCCTCC TTAACAGTGA CGTCATCCGG GCACTCCTGG 
     3361 GGGCTTCAGC ACTGGATAGC ATTCAAGAAT TCCGGCTGTC AGGGTGGTTG GCCCAGCAGG 
     3421 AAGATGCCCA TCGCATTGTG CTCTACCAAA CTGACACATC CCTGACGCCT TGGACCGTCC 
     3481 GTTGTCTGCG CCAGGCCGAC TGCATCCTCA TTGTGGGCCT GGGGGACCAG GAACCCACTG 
     3541 TTGGCCAGCT GGAGCAAATG TTAGAGAACA CTGCTGTCCG TGCCTTGAAG CAACTGGTTT 
     3601 TGCTGCACCG GGAGGAAGGC CCTGGTCCCA CGCGCACTGT AGAGTGGCTC AACATGCGCA 
     3661 GCTGGTGCTC AGGGCACCTG CACCTGCGCT GTCCTCGCCG CCTCTTCTCA CGGCGCAGCC 
     3721 CAGCGAAACT TCATGAGCTG TATGAGAAGG TTTTCTCCAG GCGTGCAGAC CGTCACAGCG 
     3781 ACTTCTCCCG CTTGGCACGA GTACTCACAG GAAACACTAT TGCTCTGGTG CTGGGTGGGG 
     3841 GCGGAGCCAG AGGCTGCTCG CATATTGGGG TGCTGAAGGC ATTAGAGGAG GCAGGAGTCC 
     3901 CAGTCGACCT TGTGGGAGGC ACATCCATAG GTTCCTTCAT TGGGGCCTTG TATGCCGAGG 
     3961 AACGCAGCGC CAGCCGAACT AAACAACGAG CCCGGGAGTG GGCCAAGAGC ATGACTTCTG 
     4021 TACTGGAGCC TGTATTGGAC CTCACATATC CTGTCACCTC CATGTTTACT GGCTCAGCCT 
     4081 TTAACAGAAG TATCCACCGT GTCTTCCAGG ATAAGCAGAT TGAGGACCTG TGGCTGCCTT 
     4141 ACTTCAATGT GACCACAGAC ATCACTGCCT CTGCCATGCG CGTCCACAAA GATGGCTCCC 
     4201 TGTGGCGGTA TGTACGTGCC AGCATGACGC TCTCGGGCTA CCTACCCCCG CTGTGCGACC 
     4261 CAAAGGATGG GCACCTGCTC ATGGATGGTG GCTACATCAA CAACCTGCCA GCGGATATTG 
     4321 CCCGAAGCAT GGGAGCCAAA ACGGTCATTG CCATCGACGT TGGAAGCCAG GATGAGACAG 
     4381 ATCTCAGCAC CTATGGGGAC AGCCTGTCTG GCTGGTGGTT GCTGTGGAAA AGGCTAAACC 
     4441 CCTGGGCAGA CAAGGTAAAG GTTCCAGACA TGGCTGAGAT CCAGTCCCGC TTGGCGTACG 
     4501 TGTCCTGTGT GCGGCAGCTA GAAGTCGTCA AGTCCAGCTC CTATTGCGAG TACCTTCGTC 
     4561 CATCCATTGA CTGCTTCAAG ACCATGGACT TTGGAAAGTT TGACCAGATC TATGATGTGG 
     4621 GTTACCAGTA TGGGAAGGCT GTCTTTGGAG GCTGGACCCG TGGTGAAGTC ATTGAGAAAA 
     4681 TGCTCACAGA CCGGAGATCT ACAGACCTTA ATGAGAGTCG CCGTGCAGAT ATACTTGCCT 
     4741 TCCCAAGTTC TGGGTTCACT GACTTGGCTG AGATTGTGTC CCGGATCGAG CCACCAACAA 
     4801 GCTACGTCTC TGATGGCTGT GCTGATGGGG AGGAGTCGGA TTGCTTGACA GAGTATGAGG 
     4861 AAGATGCAGG ACCAGACTGC TCAAGAGATG AAGGGGGCTC CCCTGAGGGT GCCAGCCCTA 
     4921 GCACTGCCTC AGAGGTGGAA GAAGAGAAGT CCACACTCCG GCAACGACGC TTTCTGCCTC 
     4981 AGGAGACTCC CAGCTCAGTC GCAGATGCCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5041 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5101 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5161 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5221 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5281 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5341 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5401 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5461 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5521 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5581 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5641 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5701 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5761 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5821 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5881 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5941 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6001 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6061 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6121 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6181 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6241 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6301 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6361 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6421 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6481 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6541 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6601 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6661 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6721 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6781 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6841 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6901 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6961 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7021 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7081 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7141 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7201 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7261 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7321 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7381 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7441 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7501 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7561 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7621 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7681 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7741 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7801 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7861 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7921 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7981 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8041 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8101 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8161 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8221 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8281 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8341 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8401 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8461 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8521 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8581 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8641 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8701 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8761 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8821 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//