LOCUS       MR225839                8019 bp    DNA     circular  SYN 
DEFINITION  Pik3cd (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase catalytic delta polypeptide (Pik3cd), transcript variant 6
ACCESSION   NM_001164051
VERSION     NM_001164051.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4176..4267 
                     /label="Myc-DDK"  
     misc_feature    4297..4882 
                     /label="PolyA signal"  
     misc_feature    4354..4373 
                     /label="Restriction Enzyme"  
     misc_feature    5158..5830 
                     /label="ColE1"  
     misc_feature    6260..7054 
                     /label="Kan/Neo"  
     misc_feature    7089..7391 
                     /label="SV40 ori"  
     misc_feature    7477..7954 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4158..4164 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4158 
                     /label="Pik3cd"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCCCTGGG GTGGACTGCC CCATGGAGTT CTGGACCAAA GAGGAGAGCC 
     1081 AGAGCGTGGT TGTTGACTTC TTGCTGCCCA CAGGGGTCTA CTTGAACTTC CCCGTGTCCC 
     1141 GCAATGCCAA CCTCAGCACC ATCAAGCAGG TGCTGTGGCA CCGTGCACAG TATGAGCCAC 
     1201 TCTTCCACAT GCTCAGTGAC CCCGAGGCCT ATGTGTTCAC CTGTGTGAAC CAGACGGCGG 
     1261 AGCAGCAGGA GTTGGAGGAT GAGCAGCGGA GGCTGTGCGA CATCCAGCCC TTCCTGCCCG 
     1321 TGCTGCGCCT CGTGGCCCGA GAGGGGGACC GCGTGAAGAA GCTCATTAAC TCCCAGATCA 
     1381 GCCTCCTCAT TGGCAAAGGT CTCCATGAGT TTGATTCCCT GCGGGACCCG GAAGTAAACG 
     1441 ACTTCCGCAC TAAGATGCGC CAGTTTTGTG AAGAGGCTGC TGCTCACCGC CAGCAGCTGG 
     1501 GCTGGGTGGA ATGGCTGCAG TACAGCTTCC CCCTGCAGCT GGAGCCCTCA GCAAGGGGTT 
     1561 GGCGGGCCGG CTTATTGCGT GTCAGCAACC GAGCCCTGCT GGTCAACGTG AAGTTCGAGG 
     1621 GCAGTGAGGA GAGCTTCACC TTCCAGGTAT CCACCAAGGA CATGCCCCTG GCACTGATGG 
     1681 CCTGTGCCCT CCGAAAAAAG GCCACAGTGT TCCGGCAGCC TCTGGTGGAG CAGCCTGAGG 
     1741 AATATGCCCT GCAGGTGAAC GGGAGGCACG AATACCTCTA CGGCAACTAC CCGCTCTGCC 
     1801 ACTTTCAGTA CATCTGCAGC TGCCTACACA GCGGGCTGAC CCCTCATCTG ACCATGGTCC 
     1861 ACTCCTCCTC CATCCTTGCT ATGCGGGATG AGCAGAGCAA TCCTGCCCCC CAAGTACAGA 
     1921 AACCACGTGC CAAACCTCCC CCGATCCCTG CCAAGAAGCC CTCCTCTGTG TCCCTGTGGT 
     1981 CCCTGGAACA GCCATTCTCC ATTGAGCTGA TCGAGGGCCG AAAAGTGAAT GCTGACGAGC 
     2041 GGATGAAGCT GGTTGTTCAG GCCGGGCTCT TCCATGGCAA TGAGATGCTG TGCAAGACTG 
     2101 TGTCAAGCTC GGAGGTGAAT GTATGCTCAG AGCCCGTGTG GAAGCAGCGA CTGGAGTTCG 
     2161 ATATCAGCGT CTGTGACCTC CCGCGCATGG CTCGACTCTG TTTTGCTCTC TATGCCGTCG 
     2221 TGGAGAAGGC TAAGAAGGCA CGCTCCACAA AGAAGAAGTC TAAGAAGGCG GACTGCCCCA 
     2281 TCGCTTGGGC CAACCTCATG CTATTCGACT ACAAAGATCA GCTCAAGACG GGGGAGCGCT 
     2341 GCCTCTACAT GTGGCCCTCT GTCCCAGATG AGAAGGGAGA GCTGCTGAAT CCTGCGGGTA 
     2401 CAGTGCGCGG GAACCCCAAC ACGGAGAGTG CCGCTGCCCT GGTCATCTAC CTGCCTGAGG 
     2461 TGGCCCCCCA CCCTGTGTAC TTCCCCGCTC TGGAGAAGAT CCTGGAGCTG GGGCGTCACG 
     2521 GGGAGCGTGG GCGCATCACG GAGGAGGAGC TGCAGCTGCG GGAGATCCTG GAACGGCGGG 
     2581 GATCCGGGGA ACTGTACGAA CATGAGAAGG ACCTGGTGTG GAAGATGCGC CACGAAGTCC 
     2641 AGGAGCATTT CCCAGAGGCG CTGGCCCGCC TGCTGCTGGT CACCAAGTGG AATAAACACG 
     2701 AGGATGTGGC CCAGCTGTCC CAGATGCTCT ATTTGCTGTG CTCCTGGCCC GAGCTGCCTG 
     2761 TGCTGAGCGC CCTGGAACTT CTGGACTTTA GCTTTCCCGA CTGCTACGTG GGCTCCTTCG 
     2821 CCATCAAGTC CCTTCGGAAG CTGACGGACG ATGAGCTCTT CCAGTACCTT CTGCAGCTGG 
     2881 TGCAAGTGCT CAAATATGAG TCCTACCTGG ACTGCGAGCT GACCAAATTC TTGCTGGGCC 
     2941 GAGCCCTGGC TAACCGCAAG ATCGGACACT TCCTGTTCTG GCACCTCCGC TCTGAGATGC 
     3001 ACGTACCATC AGTGGCTCTG CGGTTTGGTC TCATCATGGA AGCCTACTGC AGAGGCAGCA 
     3061 CCCACCACAT GAAGGTGCTG ATGAAGCAGG GGGAAGCACT GAGCAAGCTT AAGGCACTGA 
     3121 ATGACTTTGT GAAGGTGAGT TCCCAGAAGA CCACCAAGCC CCAAACCAAG GAGATGATGC 
     3181 ATATGTGCAT GCGCCAGGAG ACCTACATGG AGGCCCTGTC CCACCTGCAG TCTCCACTCG 
     3241 ACCCCAGCAC CCTGCTGGAG GAAGTCTGTG TGGAGCAGTG CACCTTCATG GACTCCAAAA 
     3301 TGAAGCCCCT GTGGATCATG TACAGCAGCG AGGAGGCGGG CAGTGCTGGC AACGTGGGCA 
     3361 TCATCTTTAA GAACGGGGAT GACCTCCGCC AGGACATGCT GACTCTGCAG ATGATCCAGC 
     3421 TCATGGACGT CCTGTGGAAG CAGGAGGGCC TGGACCTGAG GATGACGCCC TACGGCTGCC 
     3481 TCCCCACCGG GGACCGCACA GGTCTCATCG AGGTGGTCCT CCACTCGGAC ACCATCGCCA 
     3541 ACATCCAGCT GAACAAAAGC AACATGGCGG CCACAGCTGC CTTCAACAAG GACGCCCTGC 
     3601 TCAACTGGCT CAAGTCCAAG AACCCTGGGG AGGCCCTGGA TCGGGCCATT GAGGAATTCA 
     3661 CCCTCTCCTG TGCTGGCTAC TGTGTGGCCA CATATGTTCT GGGCATCGGT GACCGGCACA 
     3721 GCGACAACAT CATGATCAGA GAGAGTGGGC AGCTCTTCCA CATTGATTTT GGCCACTTTC 
     3781 TGGGGAACTT CAAGACCAAG TTTGGAATCA ACCGAGAGCG CGTCCCCTTC ATTCTCACCT 
     3841 ACGACTTTGT CCACGTGATC CAGCAGGGGA AGACTAACAA CAGTGAGAAG TTTGAAAGGT 
     3901 TCCGCGGCTA CTGTGAACGA GCCTATACCA TCCTGCGGCG CCACGGGCTG CTTTTCCTCC 
     3961 ATCTCTTCGC CCTGATGCGG GCCGCAGGTC TGCCTGAGCT TAGCTGCTCC AAAGATATCC 
     4021 AGTATCTCAA GGACTCTCTG GCACTGGGGA AGACGGAGGA AGAGGCGCTA AAGCACTTCC 
     4081 GGGTGAAGTT CAACGAAGCT CTCCGAGAAA GCTGGAAAAC CAAAGTCAAC TGGCTGGCGC 
     4141 ACAATGTGTC CAAGGATAAC CGACAGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4201 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4261 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4321 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4381 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4441 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4501 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4561 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4621 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4681 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4741 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4801 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4861 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4921 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4981 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5041 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5101 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5161 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5221 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5281 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5341 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5401 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5461 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5521 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5581 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5641 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5701 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5761 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5821 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5881 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5941 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6001 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6061 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6121 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6181 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6241 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6301 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6361 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6421 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6481 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6541 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6601 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6661 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6721 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6781 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6841 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6901 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6961 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7021 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7081 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7141 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7201 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7261 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7321 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7381 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7441 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7501 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7561 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7621 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7681 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7741 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7801 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7861 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7921 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7981 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//