LOCUS       MR225830                9378 bp    DNA     circular  SYN 
DEFINITION  Grlf1 (Myc-DDK-tagged) - Mouse glucocorticoid receptor DNA binding factor 1 (Grlf1)
ACCESSION   NM_172739
VERSION     NM_172739.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5535..5626 
                     /label="Myc-DDK"  
     misc_feature    5656..6241 
                     /label="PolyA signal"  
     misc_feature    5713..5732 
                     /label="Restriction Enzyme"  
     misc_feature    6517..7189 
                     /label="ColE1"  
     misc_feature    7619..8413 
                     /label="Kan/Neo"  
     misc_feature    8448..8750 
                     /label="SV40 ori"  
     misc_feature    8836..9313 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5517..5523 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5517 
                     /label="Arhgap35"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGATGGCA AGAAAGCAAG ATGTCCGCAT CCCCACCTAC AACATCAGTG 
     1081 TGGTGGGATT GTCAGGCACT GAGAAGGAGA AAGGCCAGTG CGGCATTGGG AAGTCTTGTC 
     1141 TGTGTAACCG CTTTGTGCGC CCAAGTGCTG ATGAGTTTCA CCTGGACCAC ACTTCTGTCC 
     1201 TTAGCACCAG TGACTTTGGT GGGCGGGTGG TCAATAATGA CCACTTTCTG TACTGGGGAG 
     1261 AAGTTAGCCG CTCCCTGGAG GATTGTGTGG AATGTAAGAT GCACATTGTG GAGCAGACTG 
     1321 AGTTTATTGA CGATCAGACT TTTCAACCTC ATCGAAGCAC GGCACTGCAG CCCTACATCA 
     1381 AGAGAGCCGC AGCCACCAAG CTTGCTTCAG CTGAAAAACT CATGTATTTT TGCACTGACC 
     1441 AGCTGGGACT GGAGCAAGAC TTTGAGCAGA AACAGATGCC AGATGGGAAG CTGCTGGTTG 
     1501 ACGGTTTCCT TCTGGGCATT GATGTTAGCA GGGGCATGAA CAGGAACTTC GATGATCAGC 
     1561 TCAAATTTGT CTCCAATCTC TACAATCAGC TTGCAAAAAC AAAAAAGCCC ATAGTGGTAG 
     1621 TCCTGACTAA GTGTGATGAG GGCGTTGAGC GGTACATTAG AGATGCACAT ACTTTTGCCT 
     1681 TAAGCAAAAA GAACCTCCAG GTTGTAGAGA CCTCTGCAAG GTCCAATGTG AATGTGGACT 
     1741 TGGCTTTCAG CACCTTAGTG CAACTCATTG ATAAGAGTCG AGGGAAGACA AAAATCATCC 
     1801 CTTACTTTGA AGCTCTCAAG CAGCAGAGTC AGCAGATAGC TACAGCAAAG GACAAGTATG 
     1861 AGTGGTTAGT GAGCCGCATT GTGAAAAATC ACAATGAGAA CTGGCCGAGT GTCAGCCGAA 
     1921 AGATGCAGGC CTCCCCTGAG TACCAGGACT ATGTCTACCT GGAAGGGACA CAGAAGGCCA 
     1981 AGAAGCTCTT CCTGCAGCAC ATTCACCGCC TCAAGCATGA GCATATTGAG CGTCGGAGAA 
     2041 AGCTGTACCT GGCAGCTCTG CCATTGGCTT TTGAAGCCCT CATACCTAAT CTAGATGAAG 
     2101 TGGACCACCT GAGCTGTATT AAAGCAAAAA AGCTGTTAGA GACCAAGCCA GAGTTCTTAA 
     2161 AGTGGTTTGT TGTACTTGAA GAAACACCAT GGGATGCCAC CAGCCACATT GACAACATGG 
     2221 AAAATGAGCG GATTCCCTTT GACTTGATGG ATACTGTCCC TGCTGAGCAG TTGTATGAGA 
     2281 CCCACTTGGA GAAGCTGAGG AACGAGAGGA AGAGAGCTGA GATGCGAAGG GCTTTCAAAG 
     2341 AAAACCTGGA GACCTCTCCT TTCATAACTC CTGGGAAACC TTGGGAAGAA GCTCGTAGTT 
     2401 TTATTATGAA TGAAGACTTC TACCAGTGGC TGGAAGAATC TGTGTACATG GACATCTATG 
     2461 GCAAGCACCA AAAGCAGATT ATAGACCGAG CAAAGGAAGA GTTCCAAGAG TTGCTTTTGG 
     2521 AGTACTCAGA ATTGTTTTAT GAGCTGGAGC TGGACGCTAA GCCCAGCAAG GAGAAGATGG 
     2581 GTGTCATCCA GGATGTTCTG GGCGAAGAGC AGAGATTTAA AGCATTGCAG AAGCTCCAAG 
     2641 CAGAGCGTGA TGCCCTCATT CTGAAGCACA TCCATTTTGT GTACCACCCG ACGAAGGAGA 
     2701 CATGCCCAAG CTGTCCAGCT TGTGTAGATG CTAAGATTGA ACACTTGATC AGTTCTCGCT 
     2761 TTATCAGACC ATCTGATCGG AATCAGAAGA ACTCTCTGTC TGACCTCAAT ATTGATAGGA 
     2821 TCAATTTGGT TATCTTAGGC AAAGATGGCC TTGCCCGAGA GTTGGCCAAT GAAATTCGAG 
     2881 CTCTTTGTAC AAATGATGAC AAGTATGTAA TAGATGGTAA AATGTATGAG CTTTCTCTGA 
     2941 GGCCAATAGA GGGGAATGTC CGGCTTCCTG TGAACTCTTT CCAGACACCA ACCTTCCAAC 
     3001 CCCATGGCTG TCTCTGCCTT TACAATTCAA AGGAGTCACT GTCCTATGTG GTGGAGAGTA 
     3061 TAGAGAAGAG CAGAGAGTCT ACACTGGGCA GGCGGGATAA TCATTTAGTC CACCTTCCCT 
     3121 TGACCTTAAT TTTAGTTAAC AAGAGAGGGG ACACAAGTGG AGAGACTCTG CACAGCTTGA 
     3181 TACAGCAAGG TCAGCAGATT GCCAGCAAAC TTCAGTGTGT CTTTCTTGAT CCGGCTTCTG 
     3241 CTGGCATTGG TTATGGACGC AACATTAATG AGAAGCAGAT CAGTCAAGTT CTGAAAGGAC 
     3301 TCCTGGACTC CAAGCGCAAC TTAAACCTGG TTAGCTCCAC TGCAAGTATC AAAGATTTGG 
     3361 CTGATGTGGA CCTTCGAATT GTCATGTGTC TGATGTGTGG TGATCCCTTT AGTGCAGATG 
     3421 ACGTTCTCTC TCCTGTCCTG CAATCCCAAA CTTGTAAATC TTCCCACTGT GGGAGCAGCA 
     3481 ACTCTGTTTT ACTTGAACTT CCAATTGGAC TACACAAGAA GCGCATTGAG CTGTCTGTTC 
     3541 TTTCATACCA TTCCTCATTT AGCATCCGAA AGAGCCGGTT GGTTCATGGG TACATTGTTT 
     3601 TTTATTCAGC CAAACGTAAG GCCTCCTTGG CAATGTTACG TGCCTTTCTT TGTGAAGTGC 
     3661 AGGATATTAT CCCCATCCAG CTTGTCGCAC TCACTGATGG CGCTATAGAT GTCCTAGACA 
     3721 ATGACTTAAG TCGAGAGCAG CTAACAGAGG GAGAGGAAAT TGCACAAGAG ATTGACGGGA 
     3781 GATTCACAAG CATCCCTTGT AGCCAGCCCC AGCATAAACT TGAGCTCTTT CATCCCTTTT 
     3841 TTAAAGATGT GGTGGAGAAA AAGAACATAA TTGAGGCCAC ACATATGTAT GATAATGTAG 
     3901 CTGAGGCCTG CAGCACCACT GAGGAAGTGT TCAACTCCCC CAGAGCTGGC TCACCCCTCT 
     3961 GCAACTCAAA CTTACAGGAC TCAGAAGAAG ATGTGGAGCC TCCATCATAC CACCTGTTTC 
     4021 GGGAAGATGC GACATTGCCC TCCCTGTCCA AAGATCATTC CAAGTTCTCT ATGGAACTGG 
     4081 AGGGAAATGA CGGGCTATCT TTCATAATGA GCAATTTTGA GAGTAAACTG AACAACAAAG 
     4141 TACCTCCCCC AGTCAAACCA AAGCCTCCTG TGCATTTTGA TATCACAAAA GATCTTTCTT 
     4201 ACTTAGACCA AGGCCATCGG GAGGGACAGA GGAAATCTAT GTCTTCTAGC CCCTGGATGC 
     4261 CTCAGGATGG GTTTGATCCT TCTGACTACG CAGAGCCCAT GGATGCTGTG GTCAAGCCAA 
     4321 GGAATGAGGA AGAAAACATA TACTCGGTGC CCCACGACAG CACCCAGGGC AAGATCATTA 
     4381 CCATTCGGAA CATCAACAAA GCCCAGTCCA ATGGCAGTGG TAATGGTTCT GACAGTGAGA 
     4441 TGGACACAAG TTCCCTAGAG CGAGGCCGCA AGGTATCTGC AGTGAGTAAG CCTGTGCTGT 
     4501 ACAGGACAAG ATGCACTCGC CTGGGGCGGT TTGCTAGTTA CCGTACCAGC TTCAGTGTCG 
     4561 GGAGTGATGA TGAGCTGGGA CCCATCCGAA AGAAAGAGGA GGACCAGGCA TCCCAAGGTT 
     4621 ATAAAGGGGA CAATGCTGTC ATTCCATATG AAACAGATGA GGACCCCAGG AGGAGGAATA 
     4681 TCCTTCGAAG TCTAAGAAGG AACACCAAGA AACCAAAGCC CAAACCCCGG CCATCCATCA 
     4741 CAAAGGCAAC CTGGGAGAGT AACTATTTTG GGGTGCCCTT AACAACAGTG GTGACTCCAG 
     4801 AGAAACCGAT ACCCATTTTC ATTGAAAGAT GCATTGAGTA CATCGAAGCC ACAGGACTAA 
     4861 GCACTGAAGG CATCTACCGG GTCAGTGGAA ACAAGTCAGA AATGGAAAGT TTGCAAAGAC 
     4921 AGTTTGATCA AGACCACAAT CTGGACCTGG CAGAGAAAGA CTTCACTGTG AACACTGTGG 
     4981 CAGGGGCCAT GAAGAGTTTT TTCTCAGAGC TACCAGACCC CCTGGTACCA TACAGCATGC 
     5041 AGATTGACTT GGTGGAAGCT CACAAAATCA ACGACAGGGA ACAGAAGCTG CATGCTCTGA 
     5101 AGGAAGTGCT GAAGAAGTTC CCGAAGGAAA ACCATGAAGT CTTCAAATAT GTCATCTCCC 
     5161 ACCTGAACAA AGTCAGCCAC AACAACAAGG TGAATCTTAT GACCAGTGAG AACCTGTCCA 
     5221 TCTGCTTCTG GCCCACGCTG ATGCGGCCTG ACTTCAGCAG CATGGACGCG CTCACAGCCA 
     5281 CGCGCTCCTA CCAGACCATC ATCGAGCTCT TCATCCAGCA GTGCCCCTTC TTCTTCTACA 
     5341 ACCGGCCCAT CAGCGAGCCT CCAGGGGCTG CGCCTGGCTC CCCTTCAGCC ATGGCTCCCA 
     5401 CTGTTCCCTT CCTCACCTCT ACACCTGCTA CCAGTCAGCC ATCACCTCCC CAGTCACCTC 
     5461 CTCCAACCCC TCAGTCCCCA ATGCAGCCAT TGCTGTCCTC TCAGCTCCAA GCCGAACACA 
     5521 CGCTGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5581 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5641 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5701 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5761 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5821 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5881 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5941 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     6001 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     6061 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     6121 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     6181 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     6241 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     6301 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     6361 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     6421 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     6481 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6541 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6601 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6661 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6721 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6781 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6841 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6901 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6961 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     7021 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     7081 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     7141 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     7201 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     7261 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     7321 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     7381 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     7441 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     7501 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7561 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7621 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7681 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7741 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7801 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7861 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7921 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7981 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     8041 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     8101 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     8161 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     8221 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     8281 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     8341 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     8401 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     8461 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     8521 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8581 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8641 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8701 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8761 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8821 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8881 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8941 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     9001 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     9061 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     9121 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     9181 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     9241 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     9301 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     9361 AAATTTAACG CGAATTTT
//