LOCUS       MR225739                8880 bp    DNA     circular  SYN 
DEFINITION  Pard3 (Myc-DDK-tagged) - Mouse par-3 (partitioning defective 3) homolog (C. elegans) (Pard3), transcript variant 3
ACCESSION   NM_033620
VERSION     NM_033620.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5037..5128 
                     /label="Myc-DDK"  
     misc_feature    5158..5743 
                     /label="PolyA signal"  
     misc_feature    5215..5234 
                     /label="Restriction Enzyme"  
     misc_feature    6019..6691 
                     /label="ColE1"  
     misc_feature    7121..7915 
                     /label="Kan/Neo"  
     misc_feature    7950..8252 
                     /label="SV40 ori"  
     misc_feature    8338..8815 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5019..5025 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5019 
                     /label="Pard3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAGTGACC GTGTGCTTCG GGAGGACCCG GGTGGTCGTG CCGTGCGGAG 
     1081 ATGGCCGCAT GAAAGTTTTC AGCCTCATCC AGCAGGCGGT GACCCGCTAC CGGAAGGCCG 
     1141 TGGCCAAGGA TCCAAACTAC TGGATACAGG TGCATCGCTT GGAGCATGGA GATGGAGGGA 
     1201 TTCTAGACCT GGATGACATC CTCTGTGACG TTGCTGATGA CAAAGACAGA CTGGTAGCAG 
     1261 TATTTGATGA ACAGGATCCC CACCATGGAG GAGATGGTAC CAGCGCCAGC TCCACGGGAA 
     1321 CCCAGAGTCC AGAGATATTC GGCAGTGAGC TGGGCACCAA CAATGTTTCT GCTTTTCAGC 
     1381 CTTATCAAGC CACAAGTGAA ATTGAGGTCA CGCCTTCAGT TCTTCGGGCA AATATGCCTC 
     1441 TTCATGTCCG CCGGAGCAGC GACCCAGCTT TAACTGGCCT TTCCACTTCT GTCAGTGATA 
     1501 ACAACTTTTC CTCAGAGGAG CCCTCCAGGA AAAACCCCAC CCGCTGGTCC ACGACAGCTG 
     1561 GCTTTCTCAA GCAGAACACC GCTGGAAGTC CCAAAACCTG CGACAGGAAG AAAGATGAAA 
     1621 ACTACAGAAG CCTTCCACGG GATCCCAGTA GCTGGTCCAA CCAGTTCCAG CGAGACAACG 
     1681 CCCGCTCCTC CCTGAGCGCC AGCCACCCAA TGGTAGACCG GTGGCTGGAG AAGCAAGAAC 
     1741 AGGATGAGGA AGGCACAGAA GAAGACAGCA GCCGAGTGGA GCCGGTTGGA CATGCTGATA 
     1801 CCGGATTGGA GAACATGCCC AACTTTTCCC TCGATGATAT GGTAAAGCTC GTACAAGTCC 
     1861 CCAACGATGG AGGGCCCCTG GGAATCCATG TAGTGCCTTT CAGTGCTCGA GGCGGCAGAA 
     1921 CATTGGGGTT GTTAGTGAAG CGGTTGGAGA AAGGCGGTAA GGCTGAGCAA GAAAACCTTT 
     1981 TCCATGAGAA TGACTGCATT GTGAGGATTA ACGATGGAGA TCTTCGAAAC AGAAGATTTG 
     2041 AGCAAGCACA ACATATGTTC CGCCAAGCTA TGCGTGCGCG TGTCATTTGG TTCCATGTGG 
     2101 TCCCTGCAGC AAACAAGGAG CAATATGAAC AACTGTCCCA ACGCGAGAAG AACAACTACT 
     2161 CCCCAGGCCG CTTCAGCCCT GACAGCCACT GTGTGGCCAA CAGGAGTGTG GCCAACAATG 
     2221 CCCCTCAAGC ATTGCCCAGA GCACCCAGAC TGAGTCAGCC ACCCGAGCAG CTGGATGCTC 
     2281 ACCCCCGACT ACCTCATAGT GCTCACGCCT CAACCAAACC ACCCGCAGCC CCGGCCTTGG 
     2341 CTCCACCCAG TGTGCTTAGT ACCAACGTAG GCAGTGTGTA CAACACGAAG AAAGTAGGCA 
     2401 AGAGGCTCAA CATCCAGCTT AAGAAAGGTA CAGAAGGACT GGGATTCAGC ATCACCTCCC 
     2461 GGGACGTCAC CATAGGTGGC TCAGCTCCCA TTTATGTCAA GAATATCCTT CCTCGAGGGG 
     2521 CTGCCATTCA GGATGGCAGA CTCAAGGCAG GAGACCGGCT AATAGAGGTC AATGGAGTAG 
     2581 ATTTAGCAGG CAAATCCCAG GAGGAAGTTG TTTCCCTGTT GAGAAGCACC AAGATGGAGG 
     2641 GGACTGTGAG CCTTCTGGTC TTTCGTCAGG AAGAGGCTTT CCACCCAAGG GAAATGAATG 
     2701 CTGAACCAAG CCAGATGCAG ACTCCAAAAG AAACGAAAGC TGAAGATGAG GACGTTGTTC 
     2761 TCACACCCGA TGGTACCAGG GAGTTTCTGA CTTTTGAAGT TCCACTGAAT GACTCAGGAT 
     2821 CTGCAGGGCT TGGTGTCAGT GTCAAGGGGA ACCGTTCCAA AGAGAACCAC GCAGATTTGG 
     2881 GGATCTTCGT TAAATCCATT ATCAATGGTG GAGCTGCATC TAAAGATGGA AGGCTGAGGG 
     2941 TAAATGACCA GCTGATAGCT GTGAATGGAG AATCTCTACT GGGCAAAGCC AACCAGGAAG 
     3001 CCATGGAGAC TCTACGGAGG TCCATGTCCA CCGAGGGCAA CAAGCGTGGC ATGATCCAGC 
     3061 TCATTGTGGC GAGGCGGATC AGCAGATGTA ACGAGCTGCG GTCTCCTGGG AGCCCTGCTG 
     3121 CACCTGAGCT GCCCATCGAG ACAGAACTGG ATGACCGAGA ACGCAGGATC TCACACTCCC 
     3181 TCTACAGTGG GATCGAGGGG CTGGATGAGT CGCCCACCAG GAACGCAGCA CTGAGCAGGA 
     3241 TAATGGGTAA ATGCCAGCTG TCCCCCACGG TGAACATGCC TCACGATGAC ACTGTCATGA 
     3301 TTGAAGATGA CAGGCTGCCT GTGCTCCCTC CTCACCTCTC TGACCAGTCC TCCTCCAGCT 
     3361 CCCATGATGA CGTTGGATTC ATAATGACAG AAGCAGGCAC GTGGGCCAAA GCTACCATCA 
     3421 GTGACTCAGC CGACTGCTCA TTGAGTCCAG ATGTTGATCC GGTTCTCGCT TTTCAACGGG 
     3481 AAGGATTTGG ACGCCAGAGT ATGTCAGAAA AACGCACAAA GCAATTTTCA GATGCCAGTC 
     3541 AATTGGATTT CGTTAAAACA CGAAAATCAA AAAGCATGGA TTTAGTAGCT GACGAGACTA 
     3601 AACTCAATAC AGTGGATGAC CAGAGAGCAG GCTCTCCCAG TAGAGATGTG GGACCCTCCT 
     3661 TAGGTCTGAA GAAATCTAGC TCCTTAGAAA GTCTGCAGAC CGCTGTCGCC GAGGTGACGC 
     3721 TGAACGGGAA CATTCCTTTC CACCGCCCTC GGCCACGAAT CATCCGGGGA AGGGGCTGCA 
     3781 ACGAAAGCTT CAGAGCCGCC ATTGACAAGT CCTACGACAA GCCCATGGTT GATGATGACG 
     3841 ACGAAGGCAT GGAGACCTTG GAAGAAGACA CAGAGGAAAG CTCGAGGTCA GGGAGGGAGT 
     3901 CCGTGTCCAC GTCCAGCGAT CAGCCTTCCT ATTCTCTGGA GAGGCAAATG AATGGAGACC 
     3961 CAGAGAAAAG GGACAAGACA GAGAGGAAAA AGGACAAAGC CGGAAAGGAT AAGAAGAAAG 
     4021 ACCGAGAGAA GGAGAAGGAT AAACTGAAAG CCAAGAAGGG GATGCTGAAA GGCTTGGGGG 
     4081 ACATGTTCAG GTTTGGCAAA CATCGAAAAG ATGACAAGAT GGAAAAAATG GGTCGAATAA 
     4141 AAATCCAGGA TTCTTTCACC TCAGAAGAGG ACAGGGTGCG GATGAAGGAA GAACAGGAGA 
     4201 GGATTCAAGC CAAAACTCGA GAGTTTAGGG AGCGGCAAGC CCGAGAGCGT GACTATGCAG 
     4261 AGATCCAAGA TTTCCATCGG ACGTTTGGCT GTGATGACGA GTTGCTGTAT GGGGGCATGT 
     4321 CATCCTATGA AGGCTGCTTG GCTCTCAATG CCAGACCCCA GAGCCCAAGA GAAGGGCACC 
     4381 TGATGGACAC TTTGTATGCC CAAGTAAAGA AACCTCGGAG CTCCAAACCT GGAGACAGCA 
     4441 ATCGATCCAC TCCTAGCAAC CATGACCGGA TACAGCGTCT ACGGCAAGAG TTCCAGCAAG 
     4501 CCAAACAGGA TGAGGATGTG GAAGACCGGC GCCGTACCTA CAGCTTTGAG CAATCTTGGT 
     4561 CCAGCTCGCG GCCGGCATCG CAGAGTGGTC GGCACTCGGT GTCCGTGGAG GTTCAAGTAC 
     4621 AACGGCAGCG CCAGGAGGAG CGAGAGAGCT TCCAGCAGGC CCAGCGCCAG TACAGCTCAC 
     4681 TGCCAAGACA AAGCAGGAAG AATGCCAGCT CCATATCACA GGATTCCTGG GAACAGAACT 
     4741 ACGCCCCTGG TGAAGGCTTC CAGAGTGCCA AGGAGAACCC CAGGTATTCC AGTTACCAGG 
     4801 GCTCAAGGAA CGGCTATCTA GGCGGGCACG GCTTTAATGC CAGGGTCATG CTGGAGACCC 
     4861 AGGAGCTTCT CCGACAGGAA CAGAGGCGGA AAGAGCAGCA GCTGAAGAAG CAGCCTCCAG 
     4921 CTGATGGAGT CAGAGGGCCC TTCCGGCAAG ATGTGCCCCC TTCTCCATCT CAGGTGGCTA 
     4981 GGCTGAACAG ACTGCAGACA CCGGAGAAAG GGCGGCCCTT CTACTCCACG CGTACGCGGC 
     5041 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     5101 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     5161 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5221 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5281 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5341 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5401 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5461 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5521 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5581 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5641 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5701 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5761 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5821 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5881 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5941 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     6001 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     6061 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     6121 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6181 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6241 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6301 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6361 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6421 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6481 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6541 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6601 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6661 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6721 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6781 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6841 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6901 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6961 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     7021 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     7081 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     7141 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7201 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7261 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7321 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7381 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7441 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7501 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7561 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7621 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7681 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7741 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7801 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7861 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7921 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7981 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     8041 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     8101 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     8161 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8221 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8281 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8341 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8401 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8461 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8521 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8581 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8641 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8701 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8761 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8821 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//