LOCUS       MR225716                8880 bp    DNA     circular  SYN 
DEFINITION  Flt1 (Myc-DDK-tagged) - Mouse FMS-like tyrosine kinase 1 (Flt1)
ACCESSION   NM_010228
VERSION     NM_010228.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5037..5128 
                     /label="Myc-DDK"  
     misc_feature    5158..5743 
                     /label="PolyA signal"  
     misc_feature    5215..5234 
                     /label="Restriction Enzyme"  
     misc_feature    6019..6691 
                     /label="ColE1"  
     misc_feature    7121..7915 
                     /label="Kan/Neo"  
     misc_feature    7950..8252 
                     /label="SV40 ori"  
     misc_feature    8338..8815 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5019..5025 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5019 
                     /label="Flt1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCAGCTGC TGGGACACCG CGGTCTTGCC TTACGCGCTG CTCGGGTGTC 
     1081 TGCTTCTCAC AGGATATGGC TCAGGGTCGA AGTTAAAAGT GCCTGAACTG AGTTTAAAAG 
     1141 GCACCCAGCA TGTCATGCAA GCAGGCCAGA CTCTCTTTCT CAAGTGCAGA GGGGAGGCAG 
     1201 CCCACTCATG GTCTCTGCCC ACGACCGTGA GCCAGGAGGA CAAAAGGCTG AGCATCACTC 
     1261 CCCCATCGGC CTGTGGGAGG GATAACAGGC AATTCTGCAG CACCTTGACC TTGGACACGG 
     1321 CGCAGGCCAA CCACACGGGC CTCTACACCT GTAGATACCT CCCTACATCT ACTTCGAAGA 
     1381 AAAAGAAAGC GGAATCTTCA ATCTACATAT TTGTTAGTGA TGCAGGGAGT CCTTTCATAG 
     1441 AGATGCACAC TGACATACCC AAACTTGTGC ACATGACGGA AGGAAGACAG CTCATCATCC 
     1501 CCTGCCGGGT GACGTCACCC AACGTCACAG TCACCCTAAA AAAGTTTCCA TTTGATACTC 
     1561 TTACCCCTGA TGGGCAAAGA ATAACATGGG ACAGTAGGAG AGGCTTTATA ATAGCAAATG 
     1621 CAACGTACAA AGAGATAGGA CTGCTGAACT GCGAAGCCAC CGTCAACGGG CACCTGTACC 
     1681 AGACAAACTA TCTGACCCAT CGGCAGACCA ATACAATCCT AGATGTCCAA ATACGCCCGC 
     1741 CGAGCCCAGT GAGACTGCTC CACGGGCAGA CTCTTGTCCT CAACTGCACC GCCACCACGG 
     1801 AGCTCAATAC GAGGGTGCAA ATGAGCTGGA ATTACCCTGG TAAAGCAACT AAGAGAGCAT 
     1861 CTATAAGGCA GCGGATTGAC CGGAGCCATT CCCACAACAA TGTGTTCCAC AGTGTTCTTA 
     1921 AGATCAACAA TGTGGAGAGC CGAGACAAGG GGCTCTACAC CTGTCGCGTG AAGAGTGGGT 
     1981 CCTCGTTCCA GTCTTTCAAC ACCTCCGTGC ATGTGTATGA AAAAGGATTC ATCAGTGTGA 
     2041 AACATCGGAA GCAGCCGGTG CAGGAAACCA CAGCAGGAAG ACGGTCCTAT CGGCTGTCCA 
     2101 TGAAAGTGAA GGCCTTCCCC TCCCCAGAAA TCGTATGGTT AAAAGATGGC TCGCCTGCAA 
     2161 CATTGAAGTC TGCTCGCTAT TTGGTACATG GCTACTCATT AATTATCAAA GATGTGACAA 
     2221 CCGAGGATGC AGGGGACTAT ACGATCTTGC TGGGCATAAA GCAGTCAAGG CTATTTAAAA 
     2281 ACCTCACTGC CACTCTCATT GTAAACGTGA AACCTCAGAT CTACGAAAAG TCCGTGTCCT 
     2341 CGCTTCCAAG CCCACCTCTC TATCCGCTGG GCAGCAGACA AGTCCTCACT TGCACCGTGT 
     2401 ATGGCATCCC TCGGCCAACA ATCACGTGGC TCTGGCACCC CTGTCACCAC AATCACTCCA 
     2461 AAGAAAGGTA TGACTTCTGC ACTGAGAATG AAGAATCCTT TATCCTGGAT CCCAGCAGCA 
     2521 ACTTAGGAAA CAGAATTGAG AGCATCTCTC AGCGCATGAC GGTCATAGAA GGAACAAATA 
     2581 AGACGGTTAG CACATTGGTG GTGGCTGACT CTCAGACCCC TGGAATCTAC AGCTGCCGGG 
     2641 CCTTCAATAA AATAGGGACT GTGGAAAGAA ACATAAAATT TTATGTCACA GATGTGCCGA 
     2701 ATGGCTTTCA CGTTTCCTTG GAAAAGATGC CAGCCGAAGG AGAGGACCTG AAACTGTCCT 
     2761 GTGTGGTCAA TAAATTCCTG TACAGAGACA TTACCTGGAT TCTGCTACGG ACAGTTAACA 
     2821 ACAGAACCAT GCACCATAGT ATCAGCAAGC AAAAAATGGC CACCACTCAA GATTACTCCA 
     2881 TCACTCTGAA CCTTGTCATC AAGAACGTGT CTCTAGAAGA CTCGGGCACC TATGCGTGCA 
     2941 GAGCCAGGAA CATATACACA GGGGAAGACA TCCTTCGGAA GACAGAAGTT CTCGTTAGAG 
     3001 ATTCGGAAGC GCCACACCTG CTTCAAAACC TCAGTGACTA CGAGGTCTCC ATCAGTGGCT 
     3061 CTACGACCTT AGACTGTCAA GCTAGAGGTG TCCCCGCGCC TCAGATCACT TGGTTCAAAA 
     3121 ACAACCACAA AATACAACAA GAACCGGGAA TTATTTTAGG ACCAGGAAAC AGCACGCTGT 
     3181 TTATTGAAAG AGTCACAGAG GAGGATGAGG GTGTCTATAG GTGCCGAGCC ACCAACCAGA 
     3241 AGGGGGCCGT GGAAAGCGCA GCCTACCTCA CCGTGCAAGG AACCTCAGAC AAGTCAAACC 
     3301 TGGAGCTGAT CACGCTCACG TGCACATGCG TGGCTGCGAC CCTCTTTTGG CTCCTTCTAA 
     3361 CTCTCTTCAT CAGAAAACTG AAGCGGTCTT CTTCCGAAGT AAAGACAGAC TACCTGTCAA 
     3421 TCATTATGGA CCCAGATGAA GTTCCCCTGG ATGAGCAGTG TGAACGGCTG CCCTATGATG 
     3481 CCAGCAAGTG GGAGTTTGCA CGGGAGAGAC TGAAACTAGG CAAATCGCTC GGAAGAGGGG 
     3541 CTTTTGGGAA AGTCGTTCAA GCCTCTGCAT TTGGCATTAA GAAATCACCC ACCTGCCGGA 
     3601 CTGTGGCTGT GAAGATGTTG AAAGAGGGGG CCACAGCCAG TGAGTACAAA GCTCTGATGA 
     3661 CCGAACTCAA GATCTTGACC CACATCGGCC ATCATCTGAA TGTGGTTAAC CTCCTGGGAG 
     3721 CCTGCACGAA GCAAGGAGGG CCTCTGATGG TGATCGTGGA ATACTGCAAA TACGGAAACC 
     3781 TGTCCAACTA CCTCAAGAGC AAACGTGACT TATTCTGTCT CAACAAGGAC GCAGCCTTGC 
     3841 ATATGGAGCT CAAGAAAGAG AGCCTGGAAC CAGGCCTGGA GCAGGGCCAG AAGCCCCGCC 
     3901 TAGACAGTGT CAGCAGCTCA AGTGTCACCA GCTCCAGCTT CCCTGAAGAC CGAAGCGTGA 
     3961 GCGATGTGGA AGGAGACGAG GATTACAGTG AGATCTCCAA GCAGCCCCTC ACCATGGAAG 
     4021 ACCTGATTTC CTACAGTTTC CAAGTGGCCA GAGGCATGGA GTTTCTGTCC TCCAGAAAGT 
     4081 GCATTCATCG GGACCTGGCA GCGAGAAACA TCCTTTTATC TGAGAACAAT GTGGTGAAGA 
     4141 TTTGCGACTT TGGCCTGGCC CGGGATATTT ATAAGAACCC TGATTATGTG AGGAGAGGAG 
     4201 ATACTCGACT TCCCCTAAAA TGGATGGCTC CTGAATCCAT CTTTGACAAG GTCTACAGCA 
     4261 CCAAGAGCGA TGTGTGGTCC TATGGCGTGT TGCTGTGGGA GATCTTCTCC TTAGGGGGTT 
     4321 CTCCATACCC AGGAGTGCAA ATGGATGAAG ACTTCTGCAG CCGCCTGAAG GAAGGCATGC 
     4381 GGATGAGAAC CCCGGAGTAT GCCACACCTG AAATCTACCA AATCATGTTG GATTGCTGGC 
     4441 ACAAAGACCC CAAAGAGAGG CCCCGGTTTG CTGAACTTGT GGAGAAACTT GGTGACCTGC 
     4501 TTCAAGCCAA CGTCCAACAG GATGGGAAAG ATTACATCCC CCTCAATGCC ATACTGACTA 
     4561 GAAACAGTGG CTTCACATAC TCGACCCCCA CCTTCTCTGA GGACCTTTTC AAGGACGGCT 
     4621 TTGCAGATCC ACATTTTCAT TCCGGAAGCT CTGATGATGT GAGATATGTA AACGCTTTCA 
     4681 AATTCATGAG CCTGGAAAGA ATCAAAACCT TTGAGGAGCT TTCACCGAAC TCCACCTCCA 
     4741 TGTTTGAGGA CTATCAGCTG GACACTAGCA CTCTGCTGGG CTCCCCCTTG CTGAAGCGGT 
     4801 TCACCTGGAC TGAGACCAAG CCCAAGGCCT CCATGAAGAT AGACTTGAGA ATAGCGAGTA 
     4861 AAAGCAAGGA GGCGGGACTT TCCGATCTGC CGAGGCCCAG CTTCTGCTTC TCCAGCTGTG 
     4921 GCCACATCAG GCCCGTGCAG GACGATGAAT CTGAGCTGGG AAAGGAGTCC TGCTGTTCTC 
     4981 CACCCCCAGA CTACAACTCC GTGGTGTTGT ACTCCTCCCC GCCCGCCACG CGTACGCGGC 
     5041 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     5101 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     5161 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5221 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5281 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5341 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5401 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5461 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5521 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5581 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5641 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5701 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5761 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5821 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5881 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5941 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     6001 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     6061 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     6121 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6181 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6241 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6301 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6361 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6421 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6481 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6541 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6601 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6661 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6721 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6781 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6841 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6901 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6961 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     7021 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     7081 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     7141 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7201 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7261 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7321 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7381 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7441 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7501 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7561 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7621 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7681 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7741 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7801 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7861 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7921 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7981 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     8041 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     8101 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     8161 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8221 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8281 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8341 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8401 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8461 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8521 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8581 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8641 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8701 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8761 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8821 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//