LOCUS       MR225676                7839 bp    DNA     circular  SYN 
DEFINITION  Ephb2 (Myc-DDK-tagged) - Mouse Eph receptor B2 (Ephb2)
ACCESSION   NM_010142
VERSION     NM_010142.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3996..4087 
                     /label="Myc-DDK"  
     misc_feature    4117..4702 
                     /label="PolyA signal"  
     misc_feature    4174..4193 
                     /label="Restriction Enzyme"  
     misc_feature    4978..5650 
                     /label="ColE1"  
     misc_feature    6080..6874 
                     /label="Kan/Neo"  
     misc_feature    6909..7211 
                     /label="SV40 ori"  
     misc_feature    7297..7774 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3978..3984 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3978 
                     /label="Ephb2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGTGCGC AGGCTGGGGG CCGCGCTGCT GCTGCTGCCG CTGCTAGCCG 
     1081 CCGTGGAAGA AACCCTGATG GACTCTACGA CAGCAACGGC TGAGCTGGGC TGGATGGTAC 
     1141 ATCCCCCATC AGGGTGGGAA GAGGTGAGCG GCTACGACGA GAACATGAAC ACTATCCGTA 
     1201 CCTACCAGGT GTGCAATGTC TTTGAGTCAA GCCAGAACAA CTGGCTGCGG ACCAAATTCA 
     1261 TCCGGCGCCG CGGCGCCCAC CGCATCCACG TGGAGATGAA GTTCTCGGTG CGTGACTGCA 
     1321 GCAGCATTCC CAGCGTGCCG GGCTCCTGCA AGGAGACCTT CAACCTCTAC TACTATGAGG 
     1381 CTGATTTTGA CTTAGCCACC AAAACCTTTC CCAACTGGAT GGAGAATCCG TGGGTGAAGG 
     1441 TGGACACCAT CGCGGCCGAT GAGAGCTTCT CTCAGGTGGA CCTGGGTGGC CGCGTCATGA 
     1501 AAATCAACAC TGAGGTGCGA AGCTTCGGTC CCGTGTCCCG CAATGGTTTC TACCTGGCCT 
     1561 TCCAGGACTA TGGCGGCTGT ATGTCCCTCA TTGCTGTGCG CGTCTTCTAC CGGAAGTGCC 
     1621 CCCGAATCAT CCAGAATGGT GCCATCTTCC AGGAGACACT GTCGGGGGCT GAGAGCACTT 
     1681 CGCTGGTGGC AGCTCGGGGC AGCTGCATCG CCAATGCTGA AGAAGTGGAT GTGCCCATCA 
     1741 AACTCTACTG TAACGGGGAC GGCGAATGGC TGGTGCCCAT AGGTCGCTGC ATGTGCAAGG 
     1801 CGGGCTTCGA GGCTGTGGAG AACGGCACCG TCTGCCGAGG TTGTCCATCA GGAACCTTCA 
     1861 AGGCCAACCA AGGGGACGAA GCCTGCACCC ACTGTCCCAT CAACAGCCGC ACCACCTCCG 
     1921 AGGGTGCCAC CAACTGTGTA TGCCGCAACG GCTACTACAG GGCCGACCTG GACCCCTTAG 
     1981 ACATGCCTTG CACAACCATC CCCTCTGCGC CCCAGGCTGT GATCTCCAGC GTCAACGAGA 
     2041 CGTCCCTCAT GCTAGAGTGG ACCCCACCCC GAGACTCGGG GGGTCGCGAG GATCTTGTTT 
     2101 ACAACATCAT CTGCAAGAGC TGTGGCTCCG GCCGGGGCGC ATGCACGCGC TGCGGGGACA 
     2161 ACGTGCAGTA CGCGCCCCGC CAGCTGGGCC TGACTGAGCC GCGCATCTAC ATCAGTGACC 
     2221 TGCTGGCACA CACGCAGTAC ACCTTCGAGA TCCAGGCCGT GAACGGTGTG ACTGACCAGA 
     2281 GTCCCTTCTC ACCTCAGTTC GCCTCTGTGA ACATCACCAC CAACCAAGCA GCACCATCGG 
     2341 CCGTGTCCAT CATGCACCAG GTGAGCCGCA CTGTGGACAG CATCACCCTG TCGTGGTCCC 
     2401 AGCCAGACCA GCCCAACGGT GTGATCCTGG ACTACGAGCT GCAGTACTAT GAGAAGGAGC 
     2461 TCAGTGAGTA CAACGCCACG GCCATAAAAA GCCCCACCAA CACAGTCACT GTGCAGGGCC 
     2521 TCAAAGCCGG CGCCATCTAT GTCTTCCAGG TGCGGGCACG CACCGTTGCA GGCTATGGGC 
     2581 GCTACAGTGG CAAGATGTAC TTCCAAACCA TGACAGAAGC CGAGTACCAG ACCAGCATCA 
     2641 AGGAAAAGCT ACCCCTCATC GTTGGCTCCT CCGCCGCCGG CTTAGTCTTC CTCATCGCTG 
     2701 TGGTCGTCAT TGCCATCGTA TGTAACAGAC GGGGGTTTGA GCGTGCCGAC TCAGAGTACA 
     2761 CGGACAAGCT ACAACACTAC ACCAGCGGAC ACATGACCCC AGGCATGAAG ATCTATATAG 
     2821 ACCCTTTCAC CTATGAAGAT CCTAATGAGG CAGTGCGGGA GTTTGCCAAG GAAATTGACA 
     2881 TCTCCTGTGT CAAGATTGAG CAGGTGATCG GAGCAGGGGA ATTTGGTGAG GTCTGCAGTG 
     2941 GCCATTTGAA GCTGCCAGGC AAGAGAGAGA TCTTTGTAGC CATCAAGACC CTCAAGTCAG 
     3001 GATACACGGA GAAACAGCGC CGGGACTTCC TGAGTGAGGC ATCCATCATG GGCCAGTTCG 
     3061 ACCACCCCAA TGTCATCCAT CTGGAAGGGG TTGTCACCAA GAGCACACCT GTCATGATCA 
     3121 TCACTGAATT CATGGAGAAC GGATCTCTGG ACTCCTTCCT CCGGCAAAAC GATGGGCAGT 
     3181 TCACAGTCAT CCAACTGGTG GGCATGCTGA GGGGCATTGC AGCCGGCATG AAGTACCTGG 
     3241 CGGACATGAA CTACGTGCAC CGTGACCTTG CTGCTCGAAA CATCCTCGTC AACAGCAACC 
     3301 TGGTGTGTAA GGTGTCTGAT TTTGGGCTCT CACGCTTCCT GGAGGATGAC ACGTCTGACC 
     3361 CCACCTATAC CAGCGCTCTG GGTGGGAAGA TCCCCATCCG TTGGACGGCA CCGGAAGCCA 
     3421 TCCAGTACCG GAAATTCACC TCGGCCAGTG ATGTGTGGAG CTATGGCATC GTCATGTGGG 
     3481 AGGTGATGTC CTACGGGGAA CGACCCTACT GGGACATGAC CAATCAAGAC GTAATCAACG 
     3541 CCATTGAACA GGACTACAGA CTACCTCCGC CCATGGACTG CCCTAGTGCC CTGCACCAGC 
     3601 TCATGCTGGA CTGCTGGCAG AAGGACCGCA ACCACCGGCC CAAGTTCGGC CAGATTGTCA 
     3661 ACACGCTGGA CAAGATGATC CGAAACCCCA ACAGCCTCAA AGCCATGGCA CCCCTGTCCT 
     3721 CTGGCATCAA CCTGCCACTG CTGGACCGCA CGATACCGGA CTACACCAGC TTTAACACGG 
     3781 TGGATGAGTG GCTAGAGGCC ATCAAGATGG GCCAGTACAA GGAGAGCTTT GCCAACGCCG 
     3841 GCTTTACCTC TTTCGACGTT GTATCTCAGA TGATGATGGA GGACATTCTC CGCGTTGGGG 
     3901 TCACTCTAGC TGGCCACCAG AAAAAAATCC TGAACAGTAT CCAGGTGATG CGGGCCCAGA 
     3961 TGAACCAGAT CCAGTCTGTA GAGGTTACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4021 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4081 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4141 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4201 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4261 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4321 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4381 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4441 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4501 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4561 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4621 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4681 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4741 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4801 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4861 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4921 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4981 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5041 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5101 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5161 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5221 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5281 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5341 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5401 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5461 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5521 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5581 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5641 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5701 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5761 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5821 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5881 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5941 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6001 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6061 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6121 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6181 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6241 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6301 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6361 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6421 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6481 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6541 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6601 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6661 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6721 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6781 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6841 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6901 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6961 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7021 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7081 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7141 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7201 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7261 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7321 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7381 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7441 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7501 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7561 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7621 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7681 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7741 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7801 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//