LOCUS       MR225666                8226 bp    DNA     circular  SYN 
DEFINITION  Nek10 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)- related kinase 10 (Nek10)
ACCESSION   NM_001195229
VERSION     NM_001195229.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4383..4474 
                     /label="Myc-DDK"  
     misc_feature    4504..5089 
                     /label="PolyA signal"  
     misc_feature    4561..4580 
                     /label="Restriction Enzyme"  
     misc_feature    5365..6037 
                     /label="ColE1"  
     misc_feature    6467..7261 
                     /label="Kan/Neo"  
     misc_feature    7296..7598 
                     /label="SV40 ori"  
     misc_feature    7684..8161 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4365..4371 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4365 
                     /label="Nek10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCGATCAA GATACAAAGG CGAAGAGCAC AGAGAAAACC GCTGATAAGC 
     1081 AACAAGGGAC CACCACCAGG GACTATTCAG ATCTTAAAAG ACTTCGGTGC CTGTTGAACG 
     1141 TTCAGTCAAG CAAACAGCAG CTTCCAGCCA TTAACTTTGA CAGTGCCCAA AATAACATGA 
     1201 CAAAGTCTGA GCCAACCATC AGGACAGGGG GACATAGAGC TCGTGGTCAA TGGCATGAGT 
     1261 CCACAGAAGC AGTTGAACTT GAAAATTTTA GTATAAACTA TAAGAATGAA AGGAATTTCA 
     1321 GCAAACATCC ACAGCATCAG TTATTTCAAG AGATCTTCAC AGCTCTGGTG AGAAACAGAC 
     1381 TTATATGCAG AGAGTGGGTT AATCGTGCTC CATCCATTCA TTTTCTGAGA GTGTTAATTT 
     1441 GTCTGAGGCT GCTGATGCGG GATCCATGTT ATCAGGAAAT ACTCCATAAA TTGGGTGGTA 
     1501 TTGAAGACCT TGCTCAGTAC ATGGAGATCG TGGCCAACGA GTACCTGGGC TACGCTGAGG 
     1561 AGCAGCACTG TGTGGACAAG CTGGTCAATA TGACGTATAT TTTTCAAAAA CTTGCTGCTG 
     1621 TGAAAGACCA AAGAGAATGG GTTACTGCAA GTGGAGCCCA CAAGACATTA GTGAGTTTGC 
     1681 TTGGTGCCCG AGACACCACT GTCCTCTTGG GTGCTCTGCT GGCACTGGCC AGCTTAGCAG 
     1741 AGAGTTCAGA GTGTAGGGAG AAGATCAGTG AACTCAACGT AGTAGAAAAT CTGTTGATGA 
     1801 TTCTGCACGA ATATGATTTA CTCTCCAAAA GACTCACTGC CGAGCTCCTG CGCCTGCTTT 
     1861 GTGCTGAGCC CCAGATCAAG GAGCAGGTGA AACTCTATGA AGGGATCCCC ATCCTGCTCA 
     1921 GTCTGCTTCA TTCTGATCAC TTGAAGCTGC TGTGGAGTGT GATCTGGATT CTGGTCCAGG 
     1981 TGTGTGAGGA CCCGGAGACT AGTGTGGAAA TCCGAATCTG GGGTGGCATC AAGCAGCTCC 
     2041 TTCATATTTT ACAAGGAGAC AGAAATTTTG TTTCTGACCG TTCTTCCATC GGAAGCCTGT 
     2101 CCAGTGCAAA TGCCGCAGGA CGCATCCAGC AGCTTCATTT GTCAGAAGAT TTAAGCCCTG 
     2161 GGGAAATCGA GGAAAATACT GTCTCTCTCC AAGCAGCCTG CTGTGCTGCC CTCACCGAGC 
     2221 TGGCCCTCAA TGATACCAAT GCCCACCAAG TGGTACAGGA AAATGGTGTA TATACAATAG 
     2281 CAAAATTAAT TTTACCAAAT AAACAATCGA ATGCAGCACA AACTAATCTA CTACAGTGTT 
     2341 ATGCTTTCAG AACCCTGAGG TTTCTCTTCA GTATGGAAAG GAACAGGCCA CTCTTTAAAA 
     2401 GACTTTTTCC CACCGACCTG TTTGAGACCT TCATTGACAT AGGACATTAC GTTCGTGATA 
     2461 TTGGTGCTTA TAAGGACTTG GTATCACAGT TGAATTTACT ATTGGAAGAT GAGCTGAAGC 
     2521 AAATTGCTGA GAATATTGAA AGCATCAATC AGAAGAAAGC GCCCTTGAAG TACATAGGTG 
     2581 ACTATGCTGT GTTGGACCAC CTTGGAAGTG GAGCTTTTGG CTGTGTTTAT AAGGTTAGAA 
     2641 AGCGTAGTGG TCAAAATCTT TTAGCAATGA AGGAGGTCAA TTTGCATAAC CCAGCATTTG 
     2701 GAAAGGATAA GAAAGACCGA GACAGCAGTG TCAAGAACAT TGTTTCTGAA TTAACAATAA 
     2761 TTAAGGAGCA GCTTTATCAT CCCAATGTTG TACGTTATTA TAAAACATTT TTGGAAAATG 
     2821 ACAGGTTGTA TATCGTTATG GAGCTGATAG AAGGAGCCCC ACTTGGAGAG CACTTTAATT 
     2881 CTTTGAAGGA AAAACATCAC CATTTCAGTG AAGAGCGACT GTGGAAAATA TTTATACAGC 
     2941 TGTGCTTAGC TCTTCGGTAT CTGCACAAGG AGAAGAGGAT TGTTCACAGA GATCTCACGC 
     3001 CAAACAACAT TATGCTGGGA GATAAGGACA AAGTGACAGT TACTGACTTT GGCCTGGCAA 
     3061 AGCAAAAACA GGAAAGCAGT AAGCTTACAT CTATGGTGGG AACGATCCTC TATTCTTGCC 
     3121 CAGAGGTCCT GAAGAGTGAG CCATATGGAG AGAAGGCCGA TGTCTGGGCT GCTGGCTGCA 
     3181 TCCTTTATCA GATGGCGACT CTAAGTCCTC CCTTCTGTAG CACCAACATG CTCTCCTTGG 
     3241 CTACTAAAAT AGTGGAGGCT GTATATGAGC CAGTGCCAGA AGGTATCTAC TCTGAAAAAG 
     3301 TGACAGATAC CATTAGAAGG TGCCTCACTC CTGATGCAGA AGCCCGTCCA GATATTGTAG 
     3361 AAGTTAGTTC CATGATATCA GATGTCATGA TGAAGTATTT AGACAGATTA TCCACATCCC 
     3421 AGCTAGCCTT GGAGAGGAAA CTGGAAAGGG AGCGGAGGCG CACACAGAGA TACTTTATGG 
     3481 AAGCCAACAG GAATGCTGTC ACATGTCACC ATGAGCTGGC TCTTCTGTCC CAGGAGACCT 
     3541 TTGAGAAGGC AAGCCTGAGC AGCAGTAGCA GCGGGGCAGC CAGCCTGAAA AGTGAGCTTT 
     3601 CAGAGAGCGC AGAGCTGCCT GGAGAGGGCT GCCACATCCC CTGCGGGAAG GAGGAGGACA 
     3661 GGGTCTGTGA GGAGGTGCTG TCAGAAGACA ACTTCCAGCT GGAGAGTGTG GAGAAAGACC 
     3721 TGTATTCTGA GCTAGACGAT GAGCTGGACG TCTCGGACAA CTGTAGCAGC TCCAGTTCAA 
     3781 GCCCCCTAAA GGAGTCTACA TTCAGCATTT TAAAGCGAAG TTTCAGTGCT TCGGGAAGAG 
     3841 AAAGGCACTC ACAGGCAAGG GACTTCATTG CTGGACTAGG ATCAAGACCA AGACCAGCAT 
     3901 CTGCAGGCAT CGCTGTATCC CAGCGGAAAG TGCGGCAGAT CTGCGACCCC ATTCAGCAGA 
     3961 TCCTGATCCA GCTACATAAA GTTATCTACA TCACGCAGCT CCCTCCAGCT CTGCACCATG 
     4021 ATCTGAAGAG AAGGGTTATA GAGAGATTCA AGAAGTCCCT GTTCAGTCAG CAGAGCAACC 
     4081 CATGTAACCT GAAATCTGAA ATTAAGAAGT TGTCTCAGGG GTCTCCAGAA CCCATCGAGC 
     4141 TGAACTTTCT TACCTCAGAT TATCATTCAC TTCGCCATTC ACGAGCTGCA AACAACTGGT 
     4201 CCCCAAGTGA CCCCACAGGT TCACCCAGCA GCTTTGAAGT GGAAGAGGGA GTCACATATG 
     4261 AGCAGATGCA GACTGTGATT GAAGAAGTCC TAGAGGAAAG TGGCTATTAC AACTTTACAA 
     4321 CTAAGAGGTG TCACTCTTTT CCATGGGTGA CCAAGAGCTA CACAGCCAAA AGAACGCGTA 
     4381 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4441 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4501 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4561 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4621 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4681 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4741 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4801 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4861 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4921 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4981 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5041 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5101 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5161 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5221 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5281 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5341 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5401 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5461 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5521 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5581 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5641 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5701 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5761 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5821 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5881 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5941 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6001 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6061 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6121 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6181 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6241 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6301 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6361 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6421 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6481 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6541 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6601 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6661 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6721 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6781 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6841 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6901 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6961 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7021 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7081 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7141 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7201 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7261 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7321 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7381 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7441 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7501 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7561 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7621 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7681 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7741 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7801 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7861 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7921 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7981 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8041 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8101 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8161 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8221 AATTTT
//