LOCUS       MR225620                7308 bp    DNA     circular  SYN 
DEFINITION  Prom1 (Myc-DDK-tagged) - Mouse prominin 1 (Prom1), transcript variant 5
ACCESSION   NM_001163582
VERSION     NM_001163582.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3465..3556 
                     /label="Myc-DDK"  
     misc_feature    3586..4171 
                     /label="PolyA signal"  
     misc_feature    3643..3662 
                     /label="Restriction Enzyme"  
     misc_feature    4447..5119 
                     /label="ColE1"  
     misc_feature    5549..6343 
                     /label="Kan/Neo"  
     misc_feature    6378..6680 
                     /label="SV40 ori"  
     misc_feature    6766..7243 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3447..3453 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3447 
                     /label="Prom1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCTCGTC TTCAGTGCCC TGCTGTTACT GGGGCTGTGT GGAAAGATCT 
     1081 CTTCAGAAGG TCAGCCTGCA TTCCATAACA CTCCTGGGGC TATGAATTAT GAATTGCCTA 
     1141 CCACCAAATA TGAGACCCAA GATACCTTCA ATGCTGGGAT TGTTGGCCCT CTCTACAAAA 
     1201 TGGTGCACAT CTTCCTCAAC GTGGTCCAGC CGAATGACTT CCCTCTAGAT TTGATCAAAA 
     1261 AACTCATACA GAACAAGAAC TTTGACATCT CAGTTGATTC CAAGGAGATT GCCCTCTATG 
     1321 AGATCGGAGT CCTTATCTGC GCCATCCTGG GACTGCTGTT CATTATCCTC ATGCCTCTGG 
     1381 TGGGCTGCTT CTTTTGTATG TGCCGTTGCT GCAACAAATG CGGCGGAGAG ATGCACCAGC 
     1441 GGCAGAAGCA GAATGCGCCA TGCAGGAGGA AGTGCTTGGG CCTCTCCCTC CTGGTGATTT 
     1501 GTCTGCTCAT GAGCCTTGGC ATTATATATG GCTTTGTGGC TAACCAGCAG ACCAGGACTC 
     1561 GGATCAAAGG GACCCAGAAA CTGGCAAAGA GCAATTTCAG AGACTTTCAA ACACTCCTGA 
     1621 CTGAAACACC AAAGCAAATT GACTATGTAG TGGAGCAGTA CACCAACACC AAGAACAAGG 
     1681 CATTCTCAGA CCTGGATGGC ATCGGCTCCG TGCTGGGAGG CAGAATAAAG GACCAACTAA 
     1741 AACCCAAAGT AACTCCTGTC CTCGAAGAGA TTAAGGCCAT GGCGACAGCC ATCAAACAGA 
     1801 CCAAGGATGC CCTGCAGAAC ATGAGCAGCA GCCTGAAAAG TCTCCAAGAT GCAGCCACCC 
     1861 AGCTCAATAC CAACCTGAGC TCTGTGAGAA ACAGCATCGA GAATTCGCTC AGCAGCAGTG 
     1921 ACTGTACCTC AGATCCAGCC AGCAAGATCT GCGATAGCAT CAGACCAAGC CTAAGCAGTC 
     1981 TGGGGAGCAG CCTCAATTCA AGTCAGGGGT ATACGACAAT TGATGAAATA CCCAATACAA 
     2041 TACAAAACCA AACTGTGGAT GTCATCAAAG ACGTCAAAAA TACCTTGGAC TCCATTAGCT 
     2101 CCAACATTAA GGACATGAGC CAAAGTATTC CTATTGAGGA TATGCTGTTA CAGGTCTCCC 
     2161 ATTACCTTAA TAACAGCAAC AGATACTTAA ACCAGGAGCT GCCCAAGCTG GAAGAATATG 
     2221 ACTCGTACTG GTGGCTGGGT GGCTTGATTG TCTGCTTTCT GCTGACTCTC ATTGTGACCT 
     2281 TCTTTTTCCT GGGCTTGCTG TGTGGTGTGT TTGGCTATGA CAAGCATGCC ACCCCAACTA 
     2341 GAAGAGGCTG TGTGTCCAAC ACTGGAGGCA TCTTCCTCAT GGCTGGGGTT GGATTCGGCT 
     2401 TCCTTTTTTG CTGGATATTG ATGATCCTTG TGGTTCTTAC GTTTGTTGTT GGTGCAAATG 
     2461 TGGAAAAGTT GCTCTGCGAA CCTTATGAAA ACAAGAAATT ATTACAGGTT TTGGACACTC 
     2521 CCTATCTGCT CAAGGAACAA TGGCAATTTT ATCTTTCTGG CATGCTATTC AATAACCCAG 
     2581 ACATTAACAT GACCTTTGAG CAAGTCTACA GGGATTGCAA AAGAGGTCGA GGTATATATG 
     2641 CTGCTTTTCA GCTTGAGAAT GTCGTCAACG TCAGTGATCA TTTCAACATT GACCAGATTT 
     2701 CTGAAAACAT AAATACGGAG TTGGAAAACC TGAATGTGAA CATTGATAGC ATTGAACTGT 
     2761 TGGATAACAC AGGAAGGAAG AGCCTCGAGG ACTTTGCACA TTCTGGGATA GATACAATCG 
     2821 ATTATTCCAC ATACTTGAAG GAGACTGAGA AATCCCCTAC TGAAGTGAAT CTGCTGACAT 
     2881 TTGCCTCTAC CCTGGAAGCA AAAGCAAACC AGTTGCCTGA AGGAAAGCTG AAACAGGCCT 
     2941 TCTTACTGGA TGTACAGAAT ATAAGAGCCA TCCACCAGCA TCTCCTCCCT CCTGTGCAGC 
     3001 AATCACTGAA TACGTTAAGA CAAAGTGTCT GGACCCTCCA GCAAACAAGC AACAAGTTGC 
     3061 CGGAGAAAGT GAAGAAGATC CTTGCCTCTT TGGACTCTGT TCAGCATTTC CTCACCAATA 
     3121 ACGTTTCCCT CATCGTTATC GGGGAAACGA AGAAGTTTGG GAAAACAATA CTAGGCTACT 
     3181 TTGAACATTA TCTGCACTGG GTCTTTTATG CCATCACAGA GAAGATGACA TCCTGCAAAC 
     3241 CCATGGCCAC CGCGATGGAC TCTGCTGTTA ATGGCATTCT GTGTGGCTAT GTTGCGGACC 
     3301 CTCTGAATTT GTTCTGGTTC GGCATAGGGA AAGCCACGGT GCTCTTACTT CCGGCTGTAA 
     3361 TCATTGCTAT CAAGCTGGCC AAGTACTATC GCAGGATGGA TTCAGAGGAT GTATACGACG 
     3421 ACTCGTCCGT CTCGGGGATG TGGCATTTCA CTTTAACGCG TACGCGGCCG CTCGAGCAGA 
     3481 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3541 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3601 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3661 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3721 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3781 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3841 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3901 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     3961 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4021 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4081 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4141 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4201 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4261 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4321 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4381 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4441 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4501 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4561 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4621 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4681 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4741 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4801 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4861 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4921 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     4981 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5041 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5101 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5161 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5221 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5281 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5341 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5401 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5461 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5521 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5581 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5641 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5701 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5761 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5821 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     5881 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     5941 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6001 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6061 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6121 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6181 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6241 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6301 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6361 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6421 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6481 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6541 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6601 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6661 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6721 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6781 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6841 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     6901 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     6961 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7021 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7081 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7141 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7201 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7261 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//