LOCUS       MR225619                7293 bp    DNA     circular  SYN 
DEFINITION  Prom1 (Myc-DDK-tagged) - Mouse prominin 1 (Prom1), transcript variant 4
ACCESSION   NM_001163581
VERSION     NM_001163581.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3450..3541 
                     /label="Myc-DDK"  
     misc_feature    3571..4156 
                     /label="PolyA signal"  
     misc_feature    3628..3647 
                     /label="Restriction Enzyme"  
     misc_feature    4432..5104 
                     /label="ColE1"  
     misc_feature    5534..6328 
                     /label="Kan/Neo"  
     misc_feature    6363..6665 
                     /label="SV40 ori"  
     misc_feature    6751..7228 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3432..3438 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3432 
                     /label="Prom1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCTCGTC TTCAGTGCCC TGCTGTTACT GGGGCTGTGT GGAAAGATCT 
     1081 CTTCAGAAGG TCAGCCTGCA TTCCATAACA CTCCTGGGGC TATGAATTAT GAATTGCCTA 
     1141 CCACCAAATA TGAGACCCAA GATACCTTCA ATGCTGGGAT TGTTGGCCCT CTCTACAAAA 
     1201 TGGTGCACAT CTTCCTCAAC GTGGTCCAGC CGAATGACTT CCCTCTAGAT TTGATCAAAA 
     1261 AACTCATACA GAACAAGAAC TTTGACATCT CAGTTGATTC CAAGGAGATC GGAGTCCTTA 
     1321 TCTGCGCCAT CCTGGGACTG CTGTTCATTA TCCTCATGCC TCTGGTGGGC TGCTTCTTTT 
     1381 GTATGTGCCG TTGCTGCAAC AAATGCGGCG GAGAGATGCA CCAGCGGCAG AAGCAGAATG 
     1441 CGCCATGCAG GAGGAAGTGC TTGGGCCTCT CCCTCCTGGT GATTTGTCTG CTCATGAGCC 
     1501 TTGGCATTAT ATATGGCTTT GTGGCTAACC AGCAGACCAG GACTCGGATC AAAGGGACCC 
     1561 AGAAACTGGC AAAGAGCAAT TTCAGAGACT TTCAAACACT CCTGACTGAA ACACCAAAGC 
     1621 AAATTGACTA TGTAGTGGAG CAGTACACCA ACACCAAGAA CAAGGCATTC TCAGACCTGG 
     1681 ATGGCATCGG CTCCGTGCTG GGAGGCAGAA TAAAGGACCA ACTAAAACCC AAAGTAACTC 
     1741 CTGTCCTCGA AGAGATTAAG GCCATGGCGA CAGCCATCAA ACAGACCAAG GATGCCCTGC 
     1801 AGAACATGAG CAGCAGCCTG AAAAGTCTCC AAGATGCAGC CACCCAGCTC AATACCAACC 
     1861 TGAGCTCTGT GAGAAACAGC ATCGAGAATT CGCTCAGCAG CAGTGACTGT ACCTCAGATC 
     1921 CAGCCAGCAA GATCTGCGAT AGCATCAGAC CAAGCCTAAG CAGTCTGGGG AGCAGCCTCA 
     1981 ATTCAAGTCA GGGGTATACG ACAATTGATG AAATACCCAA TACAATACAA AACCAAACTG 
     2041 TGGATGTCAT CAAAGACGTC AAAAATACCT TGGACTCCAT TAGCTCCAAC ATTAAGGACA 
     2101 TGAGCCAAAG TATTCCTATT GAGGATATGC TGTTACAGGT CTCCCATTAC CTTAATAACA 
     2161 GCAACAGATA CTTAAACCAG GAGCTGCCCA AGCTGGAAGA ATATGACTCG TACTGGTGGC 
     2221 TGGGTGGCTT GATTGTCTGC TTTCTGCTGA CTCTCATTGT GACCTTCTTT TTCCTGGGCT 
     2281 TGCTGTGTGG TGTGTTTGGC TATGACAAGC ATGCCACCCC AACTAGAAGA GGCTGTGTGT 
     2341 CCAACACTGG AGGCATCTTC CTCATGGCTG GGGTTGGATT CGGCTTCCTT TTTTGCTGGA 
     2401 TATTGATGAT CCTTGTGGTT CTTACGTTTG TTGTTGGTGC AAATGTGGAA AAGTTGCTCT 
     2461 GCGAACCTTA TGAAAACAAG AAATTATTAC AGGTTTTGGA CACTCCCTAT CTGCTCAAGG 
     2521 AACAATGGCA ATTTTATCTT TCTGGCATGC TATTCAATAA CCCAGACATT AACATGACCT 
     2581 TTGAGCAAGT CTACAGGGAT TGCAAAAGAG GTCGAGGTAT ATATGCTGCT TTTCAGCTTG 
     2641 AGAATGTCGT CAACGTCAGT GATCATTTCA ACATTGACCA GATTTCTGAA AACATAAATA 
     2701 CGGAGTTGGA AAACCTGAAT GTGAACATTG ATAGCATTGA ACTGTTGGAT AACACAGGAA 
     2761 GGAAGAGCCT CGAGGACTTT GCACATTCTG GGATAGATAC AATCGATTAT TCCACATACT 
     2821 TGAAGGAGAC TGAGAAATCC CCTACTGAAG TGAATCTGCT GACATTTGCC TCTACCCTGG 
     2881 AAGCAAAAGC AAACCAGTTG CCTGAAGGAA AGCTGAAACA GGCCTTCTTA CTGGATGTAC 
     2941 AGAATATAAG AGCCATCCAC CAGCATCTCC TCCCTCCTGT GCAGCAATCA CTGAATACGT 
     3001 TAAGACAAAG TGTCTGGACC CTCCAGCAAA CAAGCAACAA GTTGCCGGAG AAAGTGAAGA 
     3061 AGATCCTTGC CTCTTTGGAC TCTGTTCAGC ATTTCCTCAC CAATAACGTT TCCCTCATCG 
     3121 TTATCGGGGA AACGAAGAAG TTTGGGAAAA CAATACTAGG CTACTTTGAA CATTATCTGC 
     3181 ACTGGGTCTT TTATGCCATC ACAGAGAAGA TGACATCCTG CAAACCCATG GCCACCGCGA 
     3241 TGGACTCTGC TGTTAATGGC ATTCTGTGTG GCTATGTTGC GGACCCTCTG AATTTGTTCT 
     3301 GGTTCGGCAT AGGGAAAGCC ACGGTGCTCT TACTTCCGGC TGTAATCATT GCTATCAAGC 
     3361 TGGCCAAGTA CTATCGCAGG ATGGATTCAG AGGATGTATA CGACGACTCG TCCGTCTCGG 
     3421 GGATGTGGCA TTTCACTTTA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3481 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3541 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3601 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3661 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3721 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3781 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3841 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3901 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3961 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4021 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4081 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4141 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4201 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4261 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4321 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4381 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4441 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4501 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4561 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4621 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4681 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4741 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4801 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4861 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4921 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4981 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5041 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5101 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5161 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5221 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5281 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5341 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5401 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5461 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5521 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5581 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5641 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5701 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5761 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5821 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5881 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5941 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6001 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6061 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6121 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6181 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6241 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6301 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6361 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6421 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6481 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6541 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6601 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6661 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6721 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6781 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6841 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6901 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6961 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7021 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7081 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7141 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7201 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7261 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//