LOCUS       MR225484                8484 bp    DNA     circular  SYN 
DEFINITION  Hcn4 (Myc-DDK-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 4 (Hcn4)
ACCESSION   NM_001081192
VERSION     NM_001081192.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4641..4732 
                     /label="Myc-DDK"  
     misc_feature    4762..5347 
                     /label="PolyA signal"  
     misc_feature    4819..4838 
                     /label="Restriction Enzyme"  
     misc_feature    5623..6295 
                     /label="ColE1"  
     misc_feature    6725..7519 
                     /label="Kan/Neo"  
     misc_feature    7554..7856 
                     /label="SV40 ori"  
     misc_feature    7942..8419 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4623..4629 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4623 
                     /label="Hcn4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACAAGCTG CCGCCGTCCA TGCGCAAGCG GCTCTACAGC CTTCCGCAGC 
     1081 AGGTGGGGGC CAAGGCGTGG ATCATGGACG AGGAAGAGGA TGGTGAGGAA GAAGGGGCCG 
     1141 GGGGCCGCCA GGACCCCAGC CGAAGGAGCA TCCGGCTGCG GCCGCTGCCC TCGCCCTCTC 
     1201 CCTCGGTGGC TGCGGGCTGC TCGGAGTCCC GGGGTGCGGC CCTCGGGGCG ACAGAGAGCG 
     1261 AGGGGCCGGG CCGCAGCGCC GGCAAGTCCA GCACCAACGG TGACTGCAGG CGCTTCCGCG 
     1321 GGAGTCTGGC CTCGCTGGGC AGCCGGGGCG GCGGCAGTGG TGGAGCAGGG GGCGGCAGCA 
     1381 GTCTCGGGCA CCTGCATGAC TCCGCGGAGG AACGGCGGCT CATCGCCGCT GAGGGCGATG 
     1441 CGTCCCCCGG CGAGGACAGG ACGCCCCCGG GCCTGGCGAC CGAACCCGAG CGCCCGGCCA 
     1501 CCGCGGCACA ACCCGCAGCC TCGCCGCCGC CCCAGCAGCC GCCGCAGCCG GCCTCTGCCT 
     1561 CCTGCGAGCA GCCCTCGGCG GACACCGCTA TCAAAGTGGA GGGAGGCGCG GCCGCCAGCG 
     1621 ACCAGATCCT CCCCGAGGCC GAGGTGCGCC TGGGCCAGAG CGGCTTCATG CAGCGCCAGT 
     1681 TCGGTGCCAT GCTGCAACCT GGGGTCAACA AATTCTCCCT AAGGATGTTC GGCAGCCAGA 
     1741 AAGCGGTGGA GCGCGAGCAG GAGAGGGTTA AGTCAGCAGG GTTTTGGATT ATCCACCCCT 
     1801 ACAGTGACTT CAGATTTTAC TGGGACCTGA CGATGCTGTT GCTGATGGTG GGGAATCTGA 
     1861 TCATCATACC CGTGGGCATC ACCTTCTTCA AGGATGAGAA CACCACACCC TGGATCGTCT 
     1921 TCAATGTGGT GTCAGACACA TTCTTCCTCA TTGACTTGGT CCTCAACTTC CGCACGGGGA 
     1981 TCGTGGTGGA GGACAACACA GAAATCATCC TTGACCCGCA GAGGATCAAG ATGAAGTACC 
     2041 TGAAAAGCTG GTTTGTGGTA GATTTCATCT CCTCCATCCC TGTCGACTAC ATCTTCCTTA 
     2101 TAGTGGAGAC TCGCATTGAC TCGGAGGTCT ACAAAACCGC TAGGGCTCTG CGCATTGTCC 
     2161 GTTTCACTAA GATCCTCAGC CTCCTGCGCC TCTTGAGGCT TTCCCGCCTC ATTCGATACA 
     2221 TTCATCAGTG GGAAGAGATC TTCCACATGA CCTATGACCT GGCCAGCGCC GTGGTACGCA 
     2281 TCGTGAACCT CATTGGCATG ATGCTTCTGC TGTGTCACTG GGATGGCTGC CTGCAGTTCC 
     2341 TAGTGCCCAT GCTGCAGGAC TTCCCCCATG ACTGCTGGGT GTCCATCAAT GGCATGGTGA 
     2401 ATAACTCCTG GGGGAAGCAG TATTCCTACG CCCTCTTCAA GGCCATGAGC CACATGCTGT 
     2461 GCATTGGGTA TGGACGGCAG GCACCCGTAG GCATGTCTGA CGTCTGGCTC ACCATGCTCA 
     2521 GCATGATCGT GGGGGCCACC TGCTATGCCA TGTTCATCGG CCACGCCACT GCCCTCATCC 
     2581 AGTCGCTAGA CTCCTCCCGG CGCCAGTACC AGGAGAAGTA TAAACAGGTG GAGCAGTACA 
     2641 TGTCCTTCCA CAAGCTCCCG CCTGACACCC GACAGCGCAT CCATGACTAC TATGAACACC 
     2701 GCTACCAAGG CAAGATGTTT GATGAGGAAA GCATCCTGGG TGAGCTGAGT GAGCCACTTC 
     2761 GAGAGGAGAT CATCAACTTT AACTGCCGAA AGCTGGTGGC ATCCATGCCA CTGTTTGCCA 
     2821 ACGCAGATCC CAACTTTGTG ACATCCATGC TGACCAAGTT GCGTTTCGAG GTCTTCCAGC 
     2881 CTGGGGATTA CATCATCCGC GAAGGCACCA TCGGCAAGAA GATGTACTTT ATCCAGCACG 
     2941 GCGTGGTCAG CGTGCTCACT AAGGGCAACA AAGAGACCAA GCTGGCTGAT GGCTCCTATT 
     3001 TTGGAGAGAT CTGCTTGCTG ACCCGGGGTC GGCGCACAGC CAGCGTCAGA GCGGATACTT 
     3061 ATTGCCGCCT CTACTCACTG AGCGTGGACA ACTTCAATGA GGTGCTGGAG GAGTATCCCA 
     3121 TGATGCGGAG GGCCTTCGAG ACGGTTGCGC TGGACCGCCT GGACCGCATA GGCAAGAAGA 
     3181 ACTCCATCCT CCTCCACAAG GTGCAGCACG ACCTCAACTC AGGCGTCTTC AACTACCAAG 
     3241 AGAACGAGAT CATCCAGCAG ATCGTGCGGC ATGACCGTGA GATGGCCCAC TGTGCTCACC 
     3301 GCGTCCAGGC TGCCGCCTCA GCCACCCCAA CCCCCACGCC TGTTATATGG ACCCCGCTGA 
     3361 TCCAGGCGCC ACTGCAGGCT GCTGCTGCTA CTACTTCGGT GGCCATAGCC CTCACACACC 
     3421 ACCCCCGCCT GCCCGCCGCC ATCTTCCGGC CCCCTCCCGG ACCTGGGCTG GGCAACCTTG 
     3481 GGGCTGGACA GACACCGAGG CACCCAAGGA GGCTGCAGTC CTTGATCCCT TCAGCTCTGG 
     3541 GCTCTGCTTC ACCCGCCAGC AGCCCCTCAC AGGTGGACAC ACCGTCTTCA TCCTCCTTCC 
     3601 ACATCCAACA GCTGGCTGGA TTCTCTGCAC CTCCTGGATT GAGCCCTCTC CTGCCCTCCT 
     3661 CTAGCTCTTC CCCACCTCCA GGAGCCTGCG GTTCCCCACC AGCCCCCACA CCCTCCACCT 
     3721 CCACTGCCGC CGCCGCCTCC ACCACTGGGT TCGGCCACTT TCACAAGGCG CTGGGTGGCT 
     3781 CCCTGTCATC CTCTGACTCC CCGCTGCTCA CCCCACTGCA ACCAGGCGCT CGCTCTCCAC 
     3841 AGGCTGCCCA GCCACCACCC CCACTGCCTG GGGCCCGAGG AGGTCTGGGA CTCCTGGAGC 
     3901 ACTTCTTGCC GCCCCCACCC TCCTCCAGGT CACCATCATC CAGCCCTGGG CAGCTGGGCC 
     3961 AGCCTCCTGG AGAGTTGTCC CTAGGTCTGG CAGCTGGTCC ATCAAGTACA CCAGAGACAC 
     4021 CCCCACGGCC TGAGCGACCA TCCTTCATGG CAGGGGCCTC TGGAGGGGCT TCTCCTGTAG 
     4081 CCTTTACCCC CCGAGGAGGC CTCAGTCCTC CGGGCCACAG CCCGGGGCCC CCAAGAACTT 
     4141 TCCCGAGTGC CCCACCCCGG GCCTCTGGCT CCCATGGTTC CCTGCTCCTG CCACCTGCAT 
     4201 CCAGCCCTCC ACCTCCCCAG GTCCCACAGC GCAGGGGCAC ACCACCCCTC ACCCCTGGCC 
     4261 GCCTCACACA GGACCTGAAG CTCATCTCAG CCTCTCAGCC AGCCCTCCCC CAGGATGGGG 
     4321 CACAGACTCT CCGCAGGGCC TCGCCTCACT CCTCAGGGGA GTCGGTGGCT GCCTTCTCAC 
     4381 TCTACCCCAG AGCTGGGGGT GGCAGTGGGA GTAGTGGGGG CCTTGGGCCT CCTGGAAGGC 
     4441 CATATGGTGC CATCCCAGGC CAACATGTCA CTTTGCCTCG GAAGACATCC TCAGGTTCTT 
     4501 TGCCACCCCC ACTTTCTTTG TTTGGGGCAA GAGCCGCCTC TTCTGGAGGG CCCCCTCTGA 
     4561 CTACTGCTGC ACCCCAGAGG GAACCTGGCG CTAGGTCTGA GCCAGTACGC TCCAAACTGC 
     4621 CGTCTAATTT AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4681 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4741 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4801 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4861 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4921 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4981 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5041 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5101 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5161 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5221 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5281 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5341 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5401 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5461 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5521 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5581 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5641 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5701 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5761 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5821 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5881 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5941 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6001 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6061 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6121 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6181 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6241 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6301 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6361 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6421 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6481 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6541 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6601 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6661 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6721 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6781 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6841 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6901 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6961 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7021 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7081 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7141 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7201 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7261 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7321 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7381 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7441 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7501 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7561 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7621 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7681 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7741 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7801 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7861 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7921 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7981 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8041 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8101 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8161 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8221 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8281 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8341 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8401 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8461 TAACAAAAAT TTAACGCGAA TTTT
//