LOCUS       MR225474                7122 bp    DNA     circular  SYN 
DEFINITION  Pde4d (Myc-DDK-tagged) - Mouse phosphodiesterase 4D, cAMP specific (Pde4d)
ACCESSION   NM_011056
VERSION     NM_011056.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3279..3370 
                     /label="Myc-DDK"  
     misc_feature    3400..3985 
                     /label="PolyA signal"  
     misc_feature    3457..3476 
                     /label="Restriction Enzyme"  
     misc_feature    4261..4933 
                     /label="ColE1"  
     misc_feature    5363..6157 
                     /label="Kan/Neo"  
     misc_feature    6192..6494 
                     /label="SV40 ori"  
     misc_feature    6580..7057 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3261..3267 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3261 
                     /label="Pde4d"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAGGGAC ACTTGTGATG TACTCTCTCG GAGCAAAAGT GCCTCTGAGG 
     1081 AAACACTGCA CTCCTGTAAT GAAGAAGAGG ACCCTTTTCG TGGAATGGAG CCCTATCTCG 
     1141 TACGGCGACT TTCTTCCCGC AGCATTCAGC TTCCACCTCT GGCCTTCAGA CAGTTGGAAC 
     1201 AAGCAGACTT GCGAAGCGAA TCAGAGAACA TCCCTCGGCC AACTAGCCTC CCCTTGAAAA 
     1261 TCCTGCCTCT GATCGCCGTC ACATCTGCAG ACTCAAGTGG TTTCGATGTG GACAATGGCA 
     1321 CATCAGCGGG ACGGAGTCCC TTGGATCCCA TGACCAGCCC AGGGTCTGGG CTGATTCTCC 
     1381 AAGCAAACTT TGTCCACAGT CAACGCCGGG AGTCCTTCCT GTACCGATCT GACAGCGACT 
     1441 ATGACCTCTC TCCAAAGTCT ATGTCCAGGA ACTCCTCGAT TGCCAGTGAT ATACATGGAG 
     1501 ATGACTTGAT TGTGACCCCG TTTGCTCAGG TCTTGGCTAG CCTGCGAACC GTACGGAACA 
     1561 ACTTTGCTGC GTTAACGAAT TTGCAAGACC GAGCACCCAG CAAAAGATCA CCCATGTGCA 
     1621 ACCAACCATC CATCAACAAA GCCACCATCA CAGAGGAGGC CTACCAGAAA CTGGCCAGCG 
     1681 AGACCCTGGA GGAGCTGGAC TGGTGTCTGG ACCAGCTAGA GACCCTGCAG ACCAGGCACT 
     1741 CCGTCAGTGA GATGGCCTCC AACAAGTTCA AAAGGATGCT GAACCGGGAG CTCACGCATC 
     1801 TCTCTGAGAT GAGTCGGTCT GGCAACCAGG TGTCGGAGTA CATCTCAAAC ACATTTCTCG 
     1861 ATAAGCAACA TGAAGTGGAA ATCCCCTCTC CAACTCAGAA GGAAAAGGAG AAGAAGAAAA 
     1921 GGCCGATGTC GCAGATCAGT GGGGTCAAGA AGTTGATGCA CAGCTCCAGC CTAACTAATT 
     1981 CATGTATCCC CAGGTTTGGG GTTAAAACGG AGCAGGAAGA TGTCCTGGCC AAGGAACTAG 
     2041 AAGATGTGAA CAAGTGGGGC CTCCACGTTT TCCGAATAGC AGAGCTGTCT GGTAACCGGC 
     2101 CTCTGACTGT TATCATGCAC ACCATTTTTC AGGAACGAGA TTTGTTAAAA ACGTTTAAAA 
     2161 TCCCAGTAGA CACTTTGATT ACGTATCTTA TGACTCTGGA AGACCATTAC CATGCTGACG 
     2221 TGGCCTATCA CAACAACATC CATGCTGCAG ACGTCGTCCA GTCCACTCAC GTGCTGCTCT 
     2281 CTACACCCGC TTTGGAGGCT GTGTTCACAG ACTTGGAGAT TCTCGCGGCC ATTTTTGCCA 
     2341 GTGCAATACA TGATGTGGAC CATCCTGGGG TGTCAAATCA ATTTCTGATC AATACAAACT 
     2401 CGGAACTCGC TCTGATGTAC AACGACTCCT CGGTCCTAGA GAACCATCAC TTGGCGGTGG 
     2461 GCTTTAAGTT GCTCCAGGAA GAAAACTGTG ACATTTTCCA GAATCTGACC AAAAAGCAAA 
     2521 GACAATCTTT AAGGAAAATG GTCATTGACA TTGTCCTGGC GACAGACATG TCGAAGCACA 
     2581 TGAACCTGCT GGCTGATCTG AAAACCATGG TTGAAACCAA GAAGGTGACA AGCTCCGGCG 
     2641 TCCTCCTCCT GGATAACTAC TCCGACAGGA TCCAGGTCCT TCAGAATATG GTGCACTGTG 
     2701 CAGACCTGAG CAACCCCACA AAGCCACTCC AGCTGTACCG CCAGTGGACG GACCGGATAA 
     2761 TGGAGGAGTT CTTCCGCCAG GGGGACCGAG AGCGGGAGCG TGGCATGGAG ATAAGTCCCA 
     2821 TGTGTGACAA GCACAACGCC TCTGTGGAAA AATCACAGGT GGGCTTCATA GACTATATCG 
     2881 TTCATCCACT CTGGGAGACG TGGGCAGACC TCGTACATCC CGATGCCCAG GACATCTTGG 
     2941 ACACTTTGGA GGACAATCGT GAGTGGTACC AGAGCACAAT CCCCCAGAGC CCCTCCCCTG 
     3001 CACCTGATGA CCAAGAGGAG GGCCGGCAGG GACAGACTGA AAAATTCCAG TTTGAACTAA 
     3061 CCTTGGAGGA AGACTGCGAG TCAGACACTG AAAAGGACAG TGGAAGTCAG GTGGAGGAAG 
     3121 ACACTAGCTG CAGTGACTCT AAGACTCTGT GCACCCAAGA CTCAGAGTCC ACTGAAATTC 
     3181 CCCTTGACGA GCAGGTTGAG GAGGAAGCTG TAGCGGAAGA GGAAAGCCAG CCTGAAACTT 
     3241 GCGTCCCAGA TGACTGCTGT CCTGATACGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3301 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3361 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3421 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3481 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3541 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3601 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3661 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3721 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3781 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3841 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     3901 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     3961 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4021 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4081 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4141 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4201 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4261 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4321 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4381 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4441 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4501 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4561 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4621 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4681 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4741 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4801 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4861 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     4921 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     4981 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5041 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5101 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5161 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5221 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5281 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5341 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5401 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5461 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5521 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5581 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5641 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5701 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5761 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5821 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     5881 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     5941 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6001 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6061 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6121 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6181 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6241 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6301 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6361 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6421 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6481 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6541 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6601 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6661 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6721 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6781 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6841 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     6901 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     6961 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7021 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7081 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//