LOCUS       MR225447                8244 bp    DNA     circular  SYN 
DEFINITION  Adcy1 (Myc-DDK-tagged) - Mouse adenylate cyclase 1 (Adcy1)
ACCESSION   NM_009622
VERSION     NM_009622.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4399..4490 
                     /label="Myc-DDK"  
     misc_feature    4520..5105 
                     /label="PolyA signal"  
     misc_feature    4577..4596 
                     /label="Restriction Enzyme"  
     misc_feature    5381..6053 
                     /label="ColE1"  
     misc_feature    6483..7277 
                     /label="Kan/Neo"  
     misc_feature    7312..7614 
                     /label="SV40 ori"  
     misc_feature    7700..8177 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4374..4381 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4374 
                     /label="Adcy1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGGGCG CCGCGCGGCC AAGGCGGCGG CGGAGGCGCG GGCGAGCCCG 
     1081 GGGGCGCAGA GCGGGCGGCC GGGCCGGGCG GCCGGCGTGG GTTCCGGGCG TGTGGCGAGG 
     1141 AGTTCGCGTG CCCCGAGCTG GAGGCGCTGT TCCGCGGCTA CACGTTGCGG CTGGAGCAGG 
     1201 CGGCCACGCT GAAAGCGCTG GCTGTGCTCA GCCTACTGGC GGGCGCCCTG GCGCTGGCTG 
     1261 AGCTGCTGGG CGCACCGGGG CCAGCGCCGG GCCTCGCCAA GGGGTCGCAC CCAGTGCACT 
     1321 GCATCCTTTT CCTGGCGCTG TTCGTGGTCA CCAATGTCCG ATCCCTGCAG GTGTCCCAGC 
     1381 TGCAGCAGGT CGGTCAGCTG GCGCTGTTCT TCAGCCTCAC CTTCGCGCTA CTCTGCTGCC 
     1441 CCTTCGCGCT GGGCGGCCCC GCGAGGAGCT CTGCAGGAGG CGCAATGGGC TCGACGGTCG 
     1501 CCGAGCAGGG GGTTTGGCAA CTCCTTTTGG TCACCTTCGT GTCCTATGCC TTGCTGCCCG 
     1561 TGCGCAGCCT ACTGGCCATC GGCTTCGGGC TGGTGGTGGC TGCCTCGCAC TTACTGGTCA 
     1621 CAGCCGCCTT GGTCCCTGCC AAGCGCCCAC GTCTCTGGAG GACGCTCGGT GCCAATGCCC 
     1681 TGCTCTTCTT TGGTGTGAAC ATGTATGGCG TCTTCGTGCG GATCTTGACA GAGCGCTCCC 
     1741 AGAGGAAGGC CTTCCTGCAG GCTCGAAACT GCATTGAGGA CCGGCTGCGG CTGGAGGATG 
     1801 AGAATGAGAA GCAGGAACGG TTGCTCATGA GCCTCTTGCC CCGGAATGTC GCCATGGAGA 
     1861 TGAAGGAAGA CTTCCTGAAG CCCCCTGAGA GGATTTTTCA CAAGATTTAC ATCCAGAGGC 
     1921 ATGACAATGT GAGCATCCTG TTTGCAGACA TCGTGGGTTT CACAGGTCTG GCGTCCCAGT 
     1981 GCACAGCCCA GGAGCTGGTG AAACTGCTCA ATGAACTCTT TGGCAAGTTC GATGAGTTAG 
     2041 CCACGGAAAA CCACTGTCGC CGAATCAAGA TCCTTGGTGA CTGCTACTAC TGTGTGTCTG 
     2101 GCCTCACCCA GCCTAAGACG GATCACGCCC ACTGCTGTGT GGAGATGGGA CTTGACATGA 
     2161 TCGATACCAT CACATCCGTG GCTGAGGCTA CTGAAGTAGA CCTGAACATG CGTGTGGGCC 
     2221 TACACACCGG CAGGGTCCTC TGTGGTGTCC TGGGCCTACG AAAGTGGCAG TATGATGTAT 
     2281 GGTCCAATGA CGTGACCCTG GCCAATGTCA TGGAGGCTGC TGGCTTACCA GGGAAGGTCC 
     2341 ACATCACAAA GACGACCCTC GCGTGCTTGA ACGGGGACTA CGAGGTGGAG CCAGGTCATG 
     2401 GCCATGAGAG GAACACCTTT CTGAGAACTC ACAACATTGA GACCTTCTTT ATTGTGCCAT 
     2461 CCCATCGGAG GAAGATATTC CCAGGCTTGA TTCTCTCGGA TATCAAACCA GCCAAGAGGA 
     2521 TGAAGTTCAA GACTGTGTGC TATCTGCTGG TGCAGCTCAT GCACTGCCGG AAAATGTTCA 
     2581 AGGCTGAGAT CCCCTTCTCC AACGTGATGA CCTGCGAGGA TGACGACAAG CGGAGGGCAT 
     2641 TGAGAACAGC CTCGGAGAAG CTCAGAAACC GTTCGTCTTT CTCTACTAAT GTGGTCTACA 
     2701 CCACTCCGGG TACACGCGTC AACAGGTACA TCAGCCGCCT TCTCGAAGCC CGCCAGACAG 
     2761 AGCTGGAGAT GGCCGATCTG AACTTCTTTA CTCTGAAGTA CAAACATGTC GAACGAGAAC 
     2821 AAAAATACCA CCAGCTTCAG GACGAGTACT TCACCAGCGC CGTTGTCCTC GCCCTCATCC 
     2881 TGGCTGCCTT ATTTGGGCTT ATCTACCTTC TGGTAATCCC ACAGAGTGTG GCTGTCCTGC 
     2941 TGCTGCTGGT ATTTTCCATC TGCTTCTTGG TGGCCTGTAC CCTGTACCTG CACATCACGC 
     3001 GGGTCCAGTG TTTTCCAGGG TGCCTGACGA TCCAGATCCG CACCGCCTTG TGTGTGTTCA 
     3061 TAGTGGTCTT AATCTACTCG GTAGCTCAAG GCTGTGTGGT GGGCTGCCTG CCCTGGGCCT 
     3121 GGAGCTCTCA GTCTAACAGT TCCCTAGTGG TCCTCGCAGC TGGTGGCCGG CGCACTGTGC 
     3181 TGCCTGCCCT GCCCTGTGAG TCTGCACACC ATGCCCTGCT CTGCTGCCTG GTGGGCACCC 
     3241 TCCCACTAGC CATATTCCTG CGGGTGTCCT CCTTGCCAAA GATGATCCTG CTGTCTGGGC 
     3301 TGACTACTTC CTACATCCTT GTCCTGGAGC TCAGTGGATA CACCAAGGTT GGGGGCGGTG 
     3361 CCCTCTCTGG GCGCAGCTAT GAGCCAATCA TGGCTATCCT GCTGTTCTCA TGCACGCTGG 
     3421 CCCTGCACGC CAGGCAAGTG GATGTCAGAC TACGGCTGGA CTACCTCTGG GCAGCACAGG 
     3481 CGGAGGAGGA ACGGGATGAC ATGGAGAGAG TCAAGCTGGA CAACAAGAGG ATCCTCTTCA 
     3541 ATCTCCTGCC AGCTCACGTC GCACAGCACT TCCTCATGTC CAATCCCCGG AACATGGACC 
     3601 TCTACTACCA GTCCTACTCC CAGGTGGGCG TCATGTTTGC CTCCATCCCC AACTTCAATG 
     3661 ACTTCTACAT AGAACTGGAT GGCAACAACA TGGGTGTTGA GTGTCTGCGG CTCCTCAACG 
     3721 AGATCATTGC CGACTTTGAT GAGCTCATGG ACAAGGACTT CTATAAGGAC CTGGAGAAGA 
     3781 TTAAGACCAT TGGGAGCACT TACATGGCTG CCGTGGGGCT GGCGCCCACA GCAGGAACCA 
     3841 GGGCTAAGAA GTCCATCTCC TCCCACCTAT GCACACTGGC AGACTTCGCC ATCGACATGT 
     3901 TTGATGTTCT GGATGAAATC AACTACCAGT CCTACAACGA CTTTGTTCTC CGAGTTGGCA 
     3961 TCAACGTTGG CCCCGTGGTT GCTGGAGTGA TCGGTGCTCG CAGGCCCCAG TACGACATCT 
     4021 GGGGAAACAC AGTCAATGTG GCCAGTCGGA TGGATAGCAC TGGGGTCCAG GGACGAATTC 
     4081 AGGTGACTGA GGAAGTCCAC CGGCTGCTGA AGAGGTGCTC ATACCAGTTT GTGTGCAGAG 
     4141 GCAAAGTCAG CGTGAAGGGC AAGGGAGAGA TGCTAACGTA CTTTCTAGAA GGCAGGACTG 
     4201 ATGGAAACAG TTCCCACGGC AGGACCTTTC GCTTGGAACG AAGGATGTGT CCTTATGGGA 
     4261 GAGGCGGAGG CCAGGCCAGA CGGCCCCCAC TGTGTCCCGC GGCTGGACCC CCAGTCAGAC 
     4321 CAGGGCTCCC TCCAGCCCCC ACTAGCCAGT ACCTGTCATC CACAGCAGCA GGGAAGGAAG 
     4381 CCAGCGGACC GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4441 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4501 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4561 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4621 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4681 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4741 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4801 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4861 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4921 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4981 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5041 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5101 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5161 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5221 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5281 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5341 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5401 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5461 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5521 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5581 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5641 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5701 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5761 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5821 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5881 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5941 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6001 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6061 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6121 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6181 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6241 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6301 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6361 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6421 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6481 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6541 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6601 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6661 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6721 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6781 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6841 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6901 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6961 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7021 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7081 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7141 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7201 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7261 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7321 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7381 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7441 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7501 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7561 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7621 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7681 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7741 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7801 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7861 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7921 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7981 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8041 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8101 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8161 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8221 TAACAAAAAT TTAACGCGAA TTTT
//