LOCUS       MR225433                7404 bp    DNA     circular  SYN 
DEFINITION  Arhgap42 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 42 (Arhgap42)
ACCESSION   NM_027823
VERSION     NM_027823.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3561..3652 
                     /label="Myc-DDK"  
     misc_feature    3682..4267 
                     /label="PolyA signal"  
     misc_feature    3739..3758 
                     /label="Restriction Enzyme"  
     misc_feature    4543..5215 
                     /label="ColE1"  
     misc_feature    5645..6439 
                     /label="Kan/Neo"  
     misc_feature    6474..6776 
                     /label="SV40 ori"  
     misc_feature    6862..7339 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3543..3549 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3543 
                     /label="Arhgap42"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCTGCCC ACTCTGGAGT TCAGCGATTC GTATTTGGAC AGCCCGGATT 
     1081 TTAGGGAGCG ATTGCAGTGT CACGAGATCG AGCTGGAGAG AACCAACAAG TTCATCAAAG 
     1141 AGCTGCTGAA GGATGGCTCT CTGCTCATCG GGGCGCTGAG AAATCTGTCC ATGGCTGTAC 
     1201 AGAAGTTTTC CCAGTCTCTG CAAGACTTCC AGTTTGAGTG TATTGGAGAT GCAGAAACTG 
     1261 ATGATGAAAT TAGTATTGCT CAGTCACTGA AAGAATTCGC AAGGCTACTT ATTGCTGTAG 
     1321 AAGAAGAAAG GCGTAGACTG ATTCAAAATG CTAATGATGT ATTAATTGCA CCCCTTGAGA 
     1381 AATTTCGAAA AGAACAGATC GGTGCAGCTA AAGCAGATTC ACAGATTGGC CGAGAACATC 
     1441 AGAACTTCTA TGAAGCTTCA TTAGAATATG TCTTTAAGAT CCAAGAAGTT CAAGAGAAGA 
     1501 AGAAGTTTGA GTTTGTGGAA CCGCTTTTGT CCTTCCTTCA GGGTTTGTTT ACATTTTACC 
     1561 ACGAGGGATA TGAACTTGCC CAGGAATTTG CACCGTACAA GCAACAGCTA CAATTCAACT 
     1621 TGCAGAATAC AAGGAATAAC TTTGAAAGCA CTCGGCAAGA AGTGGAGAGA CTGATGCAGA 
     1681 GGATGAAGTC TGCCAATCAA GATTATAGAC CTCCCAGCCA GTGGACCATG GAGGGCTATC 
     1741 TTTACGTCCA GGAGAAAAGA CCACTGGGTT TCACATGGAC TAAACACTAC TGTACTTACG 
     1801 ACAAAGGAAG CAAAATGTTC ACAATGAGCG TCTCCGATGT GAAAGCCAGT GGGAAGATGA 
     1861 ACGGCCTTGT TACTGGTTCG CCTGAAATGT TTAAATTAAA GTCGTGTATC CGACGGAAGA 
     1921 CAGATTCCAT TGACAAGCGC TTCTGCTTTG ACATAGAAGT GGTCGAAAGG CATGGCATCA 
     1981 TTACGCTACA GGCATTCTCT GAAGCTAACC GGAAGCTCTG GCTGGAGGCC ATGGATGGGA 
     2041 AAGAACCGAT TTACACGTTA CCCGCCATAA TTAGCAAGAA AGAAGAAATG TACTTAAATG 
     2101 AAGCAGGCTT CAACTTTGTG AGAAAATGCA TTCAAGCTGT AGAAATGAGA GGCATCACCA 
     2161 TCTTGGGCCT GTACCGAATA GGAGGAGTGA ACTCCAAGGT CCAGAAACTC ATGAACACCA 
     2221 CATTTTCTCC CAAGTCCCCT CCTGATATGG ACATTGATAT TGAGCTGTGG GACAATAAGA 
     2281 CTATAACGAG TGGGCTGAAA AACTACCTCA GGTGCCTTGC AGAACCACTA ATGACTTACA 
     2341 AATTGCACAA GGATTTCATC ATTGCTGTCA AGTCAGACGA CCAAAACTAC AGGGTGGAGG 
     2401 CAGTCCATGC ACTGGTACAC AAATTGCCGG AGAAAAACAG AGAGATGCTG GACATCTTGA 
     2461 TAAAACACCT TCTCAAAGTA TCACTACATA GCCAACAAAA TCTTATGACC ATCTCAAATC 
     2521 TCGGTGTCAT ATTTGGTCCA ACCCTGATGA GAGCACAGGA GGAAACTGTG GCTGCCATGA 
     2581 TGAACATCAA ATTTCAGAAC ATAGTGGTGG AAATCCTAAT CGAGCACTAT GAGAAGATTT 
     2641 TTCATACTGC CCCAGACCCC AACATTCCTC TTCCTCAGCC TCAGTCTCGA TCGGGATCCC 
     2701 GAAGAACCCG GGCCATCTGC CTGTCCACAG GTTCTCGGAA GCCCAGAGGG AGGTACACGC 
     2761 CGTGCCTGGC AGAGCCTGAT AGTGACTCCT ACAGCAGCAG CCCAGACAGC ACTCCCATGG 
     2821 GCAGCATAGA GTCGCTCTCC TCCCACTCCT CTGAACAAAA CAGCACCACA AAATCCACTG 
     2881 CCTGCCAGCC CAGGGAGAAG TCAGGAGGAA TTCCCTGGAT CACAACCCCA TCATCTTCCA 
     2941 ATGGACAGAA AAGTCAGGGT CTCTGGACAA CTAGTCCAGA ATCAAGTTCT CGAGAAGATG 
     3001 CAACCAAAAC AGATGTGGAG TCAGATTGCC AGAGTGTGGC CTCTATCACA ATCCCGGGGA 
     3061 ATGTCTCTCC CCCCATAGAC CTAGTCAAAA AAGGGCCCTA TGGGCTCTCA GGACTGAAAA 
     3121 GATCCTCTGC TTCTTCCTCT CTCAGATCCA TCTCTGCAGC TGAAGGAAAC AAGAGCTACA 
     3181 GTGGATCCAT TCAAAGCTTA ACTTCTATAG GCTCCAAAGA GTCACCTAAA GCCATACCAA 
     3241 ACCCAGAGCT GCCTCCAAAA ATGTGCAGGA GGTTAAGGCT AGACACTGCC TCCAGCAATG 
     3301 GCTACCAGCG ACCTGGCTCA GTAGTGGCAG CAAAGGCTCA ACTCTTTGAA AATGCTGGGT 
     3361 CACCCAAGCC AGTTTCTTCT GGACGCCAAG CCCAAGCCAT GTACTCCTGC AAAGCCGAGC 
     3421 ACAGCCATGA GCTGTCCTTC CCCCAGGGGG CCATTTTTTC CAATGTACAC CCGTCGGTGG 
     3481 AGCCAGGGTG GCTAAAGGCA ACTTATGAAG GCAGAACAGG ACTAGTCCCT GAAAACTACG 
     3541 TTGTCTTCCT CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3601 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3661 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3721 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3781 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3841 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3901 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3961 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4021 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4081 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4141 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4201 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4261 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4321 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4381 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4441 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4501 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4561 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4621 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4681 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4741 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4801 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4861 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4921 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4981 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5041 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5101 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5161 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5221 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5281 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5341 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5401 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5461 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5521 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5581 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5641 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5701 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5761 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5821 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5881 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5941 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6001 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6061 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6121 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6181 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6241 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6301 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6361 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6421 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6481 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6541 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6601 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6661 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6721 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6781 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6841 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6901 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6961 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7021 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7081 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7141 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7201 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7261 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7321 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7381 TAACAAAAAT TTAACGCGAA TTTT
//