LOCUS       MR225382                8892 bp    DNA     circular  SYN 
DEFINITION  Trpm3 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 3 (Trpm3), transcript variant 6
ACCESSION   NM_177341
VERSION     NM_177341.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5049..5140 
                     /label="Myc-DDK"  
     misc_feature    5170..5755 
                     /label="PolyA signal"  
     misc_feature    5227..5246 
                     /label="Restriction Enzyme"  
     misc_feature    6031..6703 
                     /label="ColE1"  
     misc_feature    7133..7927 
                     /label="Kan/Neo"  
     misc_feature    7962..8264 
                     /label="SV40 ori"  
     misc_feature    8350..8827 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5031..5037 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5031 
                     /label="Trpm3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGGGCCG TGGGGGACCG TTTATTTTCT AGGCACTGCC CAGATTTGCA 
     1081 GTTTCTTGTC TTCCCGGTGG AATTTGGAAG GGGTCATGAA TCAGACTGAT GCTTCTCGAC 
     1141 CCCTAAATTG GACCATCCGG AAGCTATGCC ACGCAGCCTT TCTCCCATCT GTCAGACTTC 
     1201 TCAAGGCTCA GAAATCCTGG ATTGAAAGAG CATTTTATAA AAGAGAATGT GTTCACATCA 
     1261 TACCGAGCAC CAAAGACCCC CATAGGTGTT GCTGTGGACG CCTCATAGGC CAGCATGTTG 
     1321 GACTCACTCC CAGTATCTCT GTGCTTCAGA ATGAGAAAAA TGAGAGTCGC CTCTCCCGAA 
     1381 ATGACATCCA GTCTGAGAAG TGGTCTATCA GCAAACACAC TCAGCTCAGC CCAACAGACG 
     1441 CTTTTGGGAC CATTGAGTTC CAAGGAGGTG GCCATTCCAA CAAAGCTATG TATGTCCGAG 
     1501 TATCTTTTGA TACGAAACCT GATCTCTTGC TACACCTGAT GACCAAGGAA TGGCAACTGG 
     1561 AGCTTCCCAA ACTTCTCATC TCCGTGCACG GAGGGCTGCA GAACTTTGAA CTCCAGCCCA 
     1621 AACTCAAGCA AGTCTTCGGG AAGGGGCTCA TCAAAGCAGC CATGACAACT GGAGCCTGGA 
     1681 TCTTCACTGG AGGGGTCAAC ACAGGTGTCA TTCGTCATGT TGGAGATGCC TTGAAAGACC 
     1741 ATGCATCTAA GTCTCGAGGG AAGATATGTA CCATAGGTAT CGCCCCCTGG GGAATTGTGG 
     1801 AAAACCAGGA AGACCTGATT GGAAGAGATG TAGTCCGACC ATACCAGACC ATGTCCAATC 
     1861 CTATGAGCAA GCTCACCGTT CTCAATAGCA TGCACTCCCA CTTCATCCTG GCTGACAATG 
     1921 GGACCACGGG GAAATACGGA GCAGAGGTGA AACTCCGCAG ACAACTGGAA AAGCACATTT 
     1981 CACTCCAGAA GATAAACACA AGAATCGGTC AAGGGGTTCC AGTGGTGGCT CTCATCGTGG 
     2041 AAGGAGGACC CAATGTGATC TCAATTGTTT TGGAATACCT TCGAGACACC CCTCCTGTAC 
     2101 CAGTCGTGGT CTGTGATGGG AGTGGACGGG CATCCGACAT CCTGGCATTT GGGCATAAAT 
     2161 ATTCAGAAGA AGGCGGACTT ATCAATGAAT CTTTGAGGGA CCAGCTGTTG GTGACAATAC 
     2221 AGAAGACCTT CACGTACACT CGAACCCAAG CTCAGCACCT GTTCATCATC CTCATGGAAT 
     2281 GCATGAAGAA GAAGGAACTG ATCACAGTGT TTCGAATGGG GTCAGAAGGC CACCAGGACA 
     2341 TTGATTTAGC TATCCTGACA GCATTACTCA AAGGTGCCAA TGCATCCGCC CCAGACCAAC 
     2401 TGAGCTTAGC TTTAGCCTGG AACAGAGTTG ACATCGCTCG CAGCCAGATC TTTATTTACG 
     2461 GGCAGCAGTG GCCGGTAGGG TCCCTGGAGC AAGCCATGCT GGATGCCCTA GTCCTGGACA 
     2521 GAGTGGATTT TGTGAAATTA CTCATAGAAA ATGGAGTAAG CATGCACCGT TTTCTCACCA 
     2581 TCTCCAGACT AGAGGAACTG TACAATACGA GACATGGGCC CTCAAATACA TTGTACCACT 
     2641 TGGTCAGGGA TGTCAAAAAG CGAGAGTATC CAGGTTTCGG TTGGATCTAT TTTAAGGGAA 
     2701 ACCTGCCCCC GGACTACAGA ATCAGCCTGA TAGACATTGG CCTGGTGATC GAGTACCTGA 
     2761 TGGGCGGGGC TTACCGCTGC AACTACACGC GCAAGCGCTT TCGGACCCTC TACCACAATC 
     2821 TCTTTGGCCC CAAAAGGCCC AAAGCCTTGA AACTGCTGGG AATGGAGGAT GATATTCCCT 
     2881 TGAGGAGAGG ACGGAAGACA ACTAAGAAGC GTGAGGAAGA GGTGGACATC GACTTGGATG 
     2941 ATCCTGAGAT CAACCACTTT CCATTCCCCT TCCATGAGCT GATGGTGTGG GCCGTCCTCA 
     3001 TGAAGAGACA GAAGATGGCC CTGTTCTTCT GGCAACATGG AGAGGAGGCC ATGGCCAAAG 
     3061 CCCTGGTGGC CTGCAAGCTC TGCAAGGCCA TGGCTCACGA GGCTTCTGAG AATGACATGG 
     3121 TCGATGACAT TTCCCAGGAG CTGAACCACA ACTCCAGGGA CTTTGGCCAG CTGGCTGTGG 
     3181 AGCTCCTGGA CCAGTCCTAC AAACAGGATG AGCAGCTGGC CATGAAGCTG CTGACGTATG 
     3241 AGCTGAAGAA CTGGAGTAAT GCCACATGCC TGCAGCTGGC TGTGGCTGCC AAGCACCGTG 
     3301 ACTTCATTGC ACACACGTGC AGCCAGATGT TACTTACTGA CATGTGGATG GGACGGCTCC 
     3361 GGATGAGAAA GAACTCAGGC CTCAAGGTAA TTCTGGGAAT TCTACTTCCT CCTTCAATTC 
     3421 TCAGCTTGGA GTTCAAGAAC AAAGATGACA TGCCCTATAT GACTCAGGCC CAGGAGATTC 
     3481 ATCTCCAAGA GAAGGAGCCG GAGGAGCCAG AGAAGCCCAC AAAGGAAAAA GATGAAGAGG 
     3541 ACATGGAGCT AACAGCAATG TTGGGGCGAA GCAATGGGGA ATCATCCAGA AAGAAAGATG 
     3601 AAGAAGAAGT TCAAAGCAGG CACCGGCTAA TCCCCGTGGG CCGAAAAATC TATGAGTTCT 
     3661 ACAACGCGCC CATCGTGAAG TTCTGGTTCT ACACTCTGGC GTATATTGGA TACCTGATGC 
     3721 TCTTCAACTA CATCGTGTTA GTGAAGATGG AGCGCTGGCC TTCCACTCAG GAATGGATTG 
     3781 TCATCTCCTA CATTTTCACT CTGGGAATAG AGAAGATGAG AGAGATCCTG ATGTCGGAGC 
     3841 CGGGCAAGCT GCTGCAGAAG GTGAAGGTGT GGCTTCAGGA GTACTGGAAC GTCACAGACC 
     3901 TCATCGCCAT CCTTCTCTTC TCGGTGGGAA TGATCCTTCG TCTTCAAGAC CAGCCCTTCA 
     3961 GGAGTGACGG GAGGGTCATC TACTGTGTGA ACATCATTTA TTGGTATATC CGTTTGCTAG 
     4021 ACATCTTCGG CGTGAACAAG TATCTGGGCC CATATGTGAT GATGATTGGG AAAATGATGA 
     4081 TAGACATGAT GTACTTTGTC ATCATTATGC TGGTGGTGCT GATGAGCTTT GGGGTCGCCA 
     4141 GGCAAGCCAT TCTCTTTCCC AATGAGGAGC CATCTTGGAA ACTGGCCAAG AATATCTTCT 
     4201 ACATGCCTTA TTGGATGATT TATGGGGAAG TGTTTGCTGA CCAGATAGAC CCTCCCTGTG 
     4261 GACAGAATGA GACCCGAGAG GATGGCAAGA CAATCCAGCT GCCCCCATGC AAGACAGGAG 
     4321 CATGGATTGT CCCGGCCATA ATGGCCTGCT ACCTCTTGGT GGCGAACATC CTTCTGGTCA 
     4381 ACCTCCTTAT TGCCGTCTTC AACAATACAT TTTTTGAGGT CAAGTCGATA TCCAACCAAG 
     4441 TATGGAAATT TCAGAGGTAC CAGCTCATCA TGACTTTCCA CGAGAGGCCG GTTCTGCCCC 
     4501 CACCGCTCAT CATCTTCAGT CACATGACCA TGATCTTCCA GCATGTGTGC TGCCGGTGGA 
     4561 GGAAGCATGA GAGTGACCAG GACGAAAGGG ACTACGGCCT GAAACTCTTC ATAACTGACG 
     4621 ATGAGCTCAA GAAAGTACAC GATTTTGAAG AGCAGTGCAT AGAGGAATAT TTCCGAGAGA 
     4681 AGGATGATCG CTTCAATTCG TCCAACGATG AGAGGATACG TGTTACATCA GAAAGGGTGG 
     4741 AGAACATGTC CATGAGGCTG GAGGAAGTTA ATGAGAGAGA ACATTCCATG AAGGCTTCAC 
     4801 TCCAGACCGT GGACATCCGG CTAGCACAGC TAGAGGACCT CATCGGGCGC ATGGCCACCG 
     4861 CCCTGGAGCG CCTGACTGGT CTGGAGCGGG CAGAGTCTAA CAAAATCCGC TCAAGGACCT 
     4921 CCTCAGACTG CACAGATGCA GCCTACATCG TCCGCCAGAG CAGCTTCAAC AGCCAGGAAG 
     4981 GGAACACCTT CAAACTTCAA GAGAGTATAG ACCCTGCAGA ACATCCTTTT TATTCAGTAA 
     5041 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     5101 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5161 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5221 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5281 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5341 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5401 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5461 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5521 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5581 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5641 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5701 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5761 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5821 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5881 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5941 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6001 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6061 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6121 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6181 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6241 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6301 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6361 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6421 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6481 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6541 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6601 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6661 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6721 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6781 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6841 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6901 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6961 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     7021 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     7081 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7141 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7201 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7261 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7321 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7381 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7441 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7501 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7561 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7621 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7681 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7741 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7801 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7861 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7921 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7981 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     8041 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     8101 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8161 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8221 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8281 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8341 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8401 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8461 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8521 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8581 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8641 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8701 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8761 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8821 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8881 AACGCGAATT TT
//