LOCUS       MR225327                8250 bp    DNA     circular  SYN 
DEFINITION  Tek (Myc-DDK-tagged) - Mouse endothelial-specific receptor tyrosine kinase (Tek)
ACCESSION   NM_013690
VERSION     NM_013690.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4407..4498 
                     /label="Myc-DDK"  
     misc_feature    4528..5113 
                     /label="PolyA signal"  
     misc_feature    4585..4604 
                     /label="Restriction Enzyme"  
     misc_feature    5389..6061 
                     /label="ColE1"  
     misc_feature    6491..7285 
                     /label="Kan/Neo"  
     misc_feature    7320..7622 
                     /label="SV40 ori"  
     misc_feature    7708..8185 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4389..4395 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4389 
                     /label="Tek"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACTCTTTA GCCGGCTTAG TTCTCTGTGG AGTCAGCTTG CTCCTTTATG 
     1081 GAGTAGTAGA AGGCGCCATG GACCTGATCT TGATCAATTC CCTACCTCTT GTGTCTGATG 
     1141 CCGAAACATC CCTCACCTGC ATTGCCTCTG GGTGGCACCC CCATGAGCCC ATCACCATAG 
     1201 GAAGGGACTT TGAAGCCTTA ATGAACCAGC ACCAAGATCC ACTGGAGGTT ACTCAAGATG 
     1261 TGACCAGAGA ATGGGCGAAA AAAGTTGTTT GGAAGAGAGA AAAGGCCAGT AAGATTAATG 
     1321 GTGCTTATTT CTGTGAAGGT CGAGTTCGAG GACAGGCTAT AAGGATACGG ACCATGAAGA 
     1381 TGCGTCAACA AGCATCCTTC CTACCTGCTA CTTTAACTAT GACCGTGGAC AGGGGAGATA 
     1441 ATGTGAACAT ATCTTTCAAA AAGGTGTTAA TTAAAGAAGA AGATGCAGTG ATTTACAAAA 
     1501 ATGGCTCCTT CATCCACTCA GTGCCCCGGC ATGAAGTACC TGATATTTTA GAAGTTCACT 
     1561 TGCCGCATGC TCAGCCCCAG GATGCTGGTG TGTACTCGGC CAGGTACATA GGAGGAAACC 
     1621 TGTTCACCTC AGCCTTCACC AGGCTGATTG TTCGGAGATG TGAAGCTCAG AAGTGGGGGC 
     1681 CCGACTGTAG CCGTCCTTGT ACTACTTGCA AGAACAATGG AGTCTGCCAT GAAGATACCG 
     1741 GGGAATGCAT TTGCCCTCCT GGGTTTATGG GGAGAACATG TGAGAAAGCT TGTGAGCCGC 
     1801 ACACATTTGG CAGGACCTGT AAAGAAAGGT GTAGTGGACC AGAAGGATGC AAGTCTTATG 
     1861 TGTTCTGTCT CCCAGACCCT TACGGGTGTT CCTGTGCCAC AGGCTGGAGG GGGTTGCAGT 
     1921 GCAATGAAGC ATGCCCATCT GGTTACTACG GACCAGACTG TAAGCTCAGG TGCCACTGTA 
     1981 CCAATGAAGA GATATGTGAT CGGTTCCAAG GATGCCTCTG CTCTCAAGGA TGGCAAGGGC 
     2041 TGCAGTGTGA GAAAGAAGGC AGGCCAAGGA TGACTCCACA GATAGAGGAT TTGCCAGATC 
     2101 ACATTGAAGT AAACAGTGGA AAATTTAACC CCATCTGCAA AGCCTCTGGG TGGCCACTAC 
     2161 CTACTAGTGA AGAAATGACC CTAGTGAAGC CAGATGGGAC AGTGCTCCAA CCAAATGACT 
     2221 TCAACTATAC AGATCGTTTC TCAGTGGCCA TATTCACTGT CAACCGAGTC TTACCTCCTG 
     2281 ACTCAGGAGT CTGGGTCTGC AGTGTGAACA CAGTGGCTGG GATGGTGGAA AAGCCTTTCA 
     2341 ACATTTCCGT CAAAGTTCTT CCAGAGCCCC TGCACGCCCC AAATGTGATT GACACTGGAC 
     2401 ATAACTTTGC TATCATCAAT ATCAGCTCTG AGCCTTACTT TGGGGATGGA CCCATCAAAT 
     2461 CCAAGAAGCT TTTCTATAAA CCTGTCAATC AGGCCTGGAA ATACATTGAA GTGACGAATG 
     2521 AGATTTTCAC TCTCAACTAC TTGGAGCCGC GGACTGACTA CGAGCTGTGT GTGCAGCTGG 
     2581 CCCGTCCTGG AGAGGGTGGA GAAGGGCATC CTGGGCCTGT GAGACGATTT ACAACAGCGT 
     2641 CTATCGGACT CCCTCCTCCA AGAGGTCTCA GTCTCCTGCC AAAAAGCCAG ACAGCTCTAA 
     2701 ATTTGACTTG GCAACCGATA TTTACAAACT CAGAAGATGA ATTTTATGTG GAAGTCGAGA 
     2761 GGCGATCCCT GCAAACAACA AGTGATCAGC AGAACATCAA AGTGCCTGGG AACCTGACCT 
     2821 CGGTGCTACT GAGCAACTTA GTCCCCAGGG AGCAGTACAC AGTCCGAGCT AGAGTCAACA 
     2881 CCAAGGCGCA GGGGGAGTGG AGTGAAGAAC TCAGGGCCTG GACCCTTAGT GACATTCTCC 
     2941 CTCCTCAACC AGAAAACATC AAGATCTCCA ACATCACTGA CTCCACAGCT ATGGTTTCTT 
     3001 GGACAATAGT GGATGGCTAT TCGATTTCTT CCATCATCAT CCGGTATAAG GTTCAGGGCA 
     3061 AAAATGAAGA CCAGCACATT GATGTGAAGA TCAAGAATGC TACCGTTACT CAGTACCAGC 
     3121 TCAAGGGCCT AGAGCCAGAG ACTACATACC ATGTGGATAT TTTTGCTGAG AACAACATAG 
     3181 GATCAAGCAA CCCAGCCTTT TCTCATGAAC TGAGGACGCT TCCACATTCC CCAGCCTCTG 
     3241 CAGACCTCGG AGGGGGAAAG ATGCTACTCA TAGCCATCCT TGGGTCGGCT GGAATGACTT 
     3301 GCATCACCGT GCTGTTGGCG TTTCTGATTA TGTTGCAACT GAAGAGAGCA AATGTCCAAA 
     3361 GGAGAATGGC TCAGGCATTC CAGAACGTGA GAGAAGAACC AGCTGTGCAG TTTAACTCAG 
     3421 GAACTCTGGC CCTTAACAGG AAGGCCAAAA ACAATCCGGA TCCCACAATT TATCCTGTGC 
     3481 TTGACTGGAA TGACATCAAG TTTCAAGACG TGATCGGAGA GGGCAACTTT GGCCAGGTTC 
     3541 TGAAGGCACG CATCAAGAAG GATGGGTTAC GGATGGATGC CGCCATCAAG AGGATGAAAG 
     3601 AGTATGCCTC CAAAGATGAT CACAGGGACT TCGCAGGAGA ACTGGAGGTT CTTTGTAAAC 
     3661 TTGGACACCA TCCAAACATC ATTAATCTCT TGGGAGCATG TGAACACCGA GGCTATTTGT 
     3721 ACCTAGCTAT TGAGTATGCC CCGCATGGAA ACCTCCTGGA CTTCCTGCGT AAGAGCAGAG 
     3781 TGCTAGAGAC AGACCCTGCT TTTGCCATCG CCAACAGTAC AGCTTCCACA CTGTCCTCCC 
     3841 AACAGCTTCT TCATTTTGCT GCAGATGTGG CCCGGGGGAT GGACTACTTG AGCCAGAAAC 
     3901 AGTTTATCCA CAGGGACCTG GCTGCCAGAA ACATTTTAGT TGGTGAAAAC TACATAGCCA 
     3961 AAATAGCAGA TTTTGGATTG TCACGAGGTC AAGAAGTGTA TGTGAAAAAG ACAATGGGAA 
     4021 GGCTCCCAGT GCGTTGGATG GCAATCGAAT CACTGAACTA TAGTGTCTAT ACAACCAACA 
     4081 GTGATGTCTG GTCCTATGGT GTATTGCTCT GGGAGATTGT TAGCTTAGGA GGCACCCCCT 
     4141 ACTGCGGCAT GACGTGCGCG GAGCTCTATG AGAAGCTACC CCAGGGCTAC AGGCTGGAGA 
     4201 AGCCCCTGAA CTGTGATGAT GAGGTGTATG ATCTAATGAG ACAGTGCTGG AGGGAGAAGC 
     4261 CTTATGAGAG ACCATCATTT GCCCAGATAT TGGTGTCCTT AAACAGGATG CTGGAAGAAC 
     4321 GGAAGACATA CGTGAACACC ACACTGTATG AGAAGTTTAC CTATGCAGGA ATTGACTGCT 
     4381 CTGCGGAAGA AGCAGCCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4441 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4501 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4561 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4621 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4681 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4741 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4801 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4861 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4921 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4981 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5041 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5101 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5161 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5221 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5281 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5341 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5401 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5461 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5521 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5581 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5641 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5701 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5761 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5821 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5881 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5941 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6001 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6061 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6121 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6181 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6241 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6301 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6361 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6421 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6481 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6541 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6601 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6661 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6721 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6781 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6841 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6901 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6961 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7021 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7081 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7141 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7201 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7261 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7321 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7381 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7441 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7501 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7561 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7621 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7681 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7741 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7801 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7861 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7921 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7981 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8041 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8101 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8161 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8221 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//